Abstract_Japan-Thai Confernces 2.pdf

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Preface
6th
Japan-Thailand International High School Project Conference (2023)
The primary objective of the International High School Project Conference (2023), held
on October 28, 2023, is to promote a scientific exchange between students from Thailand and
Japan and bring together academics and young scientists in the area of science and technology.
The 6th
International High School Project Conference has attracted more than 200 participants
from four Japanese high schools and three Thai high schools. There are 87 oral and poster
presentations in areas of biology and environment, chemistry, mathematics and statistics,
physics and astronomy, technology and computer, and social science.
I would like to thank all the conference participants from both Japan and Thailand,
especially Tokai University Takanawadai Senior High School, for the warm welcome.
I would like to express my deep appreciation and thank all the members of Tokai
University Takanawadai Senior High School for their tremendous efforts. I hope that all of the
students will enjoy this conference. I look forward to seeing all of you next year at the
conference.
Associate Professor Preeyanan Sanpote
Director of Demonstration School,
University of Phayao
P.
Sampote

Preface
6th
Japan-Thailand International High School Project Conference (2023)
I am glad that we hold the 6th
Japan-Thailand High School Academic Conference, 2023.
Tokai University Takanawadai Senior High School has been designated Super Science High
School by MEXT (The Ministry of Education, Culture, Sports, Science and Technology) since
2004.
Originally this conference was held as the event in which our school reports the
achievement of each goal we made of SSH activities. Later several Japanese schools which
our school has close ties with came to participate. Year by year the number of participating
schools are increasing and since 2016 Demonstration School of University of Phayao has
participated, in addition, in 2018 Mahasarakham University Demonstration School joined. In
2023, from Thailand these two high schools and Demonstration School of Thaksin University,
3 Japanese high schools, [ Niigata Prefectural Shibata High School, Yamagata Prefectural
Touohgakkan Senior High School, Hyogo Prefectural Touooka High School gather and make
presentations about their own projects.
I hope that all the participants will get a lot of meaningful things through this
conference. Moreover, I would like the students to talk to one another and to make good
friendship.
Lastly, I hope that the 6th Japan-Thailand High School Academic Conference will
succeed and this event will continue.
Katagiri Tomomichi
Principal

TABLE OF CONTENT
Page
BIOLOGY AND ENVIRONMENT
BE-01 Evaluating the Polyphenol Content of Coffee Extracted
using Hot Water Versus Ethanol 1
BE-02 Comparison of Polyphenol Content of Green Tea Extracted
by Hot Water and Ethanol 2
BE-03 Creating Phyto Stem Cell Growth Through Tissue Culture
of Carnivorous Plants for Future Advancement 3
BE-04 Seaching for the Strategies to Enhance Flavonoid Contents
Houttuynia Cordata (Plukaow) 4
BE-05 Seed Germination Patterns and Micropropagation of Prosthechea
Fragrans in Vitro 5
BE-06 The Effect of Media on Growth of Dendrobium Friedericksianum Rchb.F. 6
BE-07 Anatomical Analysis of the Vegetative Parts of Gardenia
Sootepensis Hutch. (Rubiaceae) 7
BE-08 Effects of Artificial Habitats On Density and Species of Aquatic Insects 8
BE-09 Effect of The Fermeted Almond Leaves Solution On the Color
Of Golden Fish 9
BE-10 Bioinformatics in Plants Medicine for Anti-Cancer, Anti-Virus and Anti-Allergy 10
BE-11 Molecular Characterization of Novel Transforming Growth
Factor Beta 2 (Tgf-Β2) Gene in Glyphoglossus Molossus 11
BE-12 Alteration of The Bacterial Community in Infected Fighting Fish
(Betta Splendens) 12
BE-13 Situational Anxiety of Successful Futsal Athletes
in the 45th
Demonstration Games 13
BE-14 Anticancer Effects of Harrisonia Perforata (Blanco) Merr., Streblus Asper Lour.
and Areca Catechu L. Ethanolic Extracts on Human Oral Cancer Cell Line Hsc-7 14
BE-15 Preparation of Kombucha Synbiotic Gummy from Green Tea
Infused Shatavari Tea Plus Inulin from Yam Bean 15

TABLE OF CONTENT
Page
BIOLOGY AND ENVIRONMENT
BE-16 Method Validation for Microplastic Extraction from Northeastern
Thailand Fermented Fish Sauce by Hydrogen Peroxide and Temperature 16
BE-17 Screening of Extracellular L-Asparagenase Producing Bacteria
from Rhizospheric Soil and Roots of Euphorbia Hirta L. 17
BE-18 Using Herbal Detergents for Dust Mite Elimination 18
BE-19 Film Forming Spray of Cissampelos Pareira Extract and Piper Sarmentosum
Extract Against Bacteria 19
BE-20 A Study on the Response of Tribolium Castaneum to Ultrasonic Waves 20
BE-21 Development of Semi-Cross Flow Biofilter System for Water Filtration
in Ornamental Fish Aquarium 21
BE-22 Four Types of Amino Acids: Comparison of Bacterial Growth 22
BE-23 Investigating the Relationship between Killifish Growth and Protein Solutions 23
BE-24 Population Changes of Euglena in Different Environments 24
BE-25 Growth Rates of Medaka 25
BE-26 Relationship between the Presence of Wood Chips and Soil Biota 26
BE-27 Comparison of Colony Growth with Different Kinds of Drinks 27
BE-28 Southern Pond Shrimp and Food Types 28
BE-29 The Relationship between Light Color and Growth of Shiitake Mushrooms 29
BE-30 Finding Substances that Inhibit Yeast Growth 30
BE-31 Water and Microorganisms 31
BE-32 Regeneration Rate of Planarian Flatworms Due to Changes in Water Quality 32
BE-33 Growth of Killifish in Different Environments 33
BE-34 The Effects of Weather and Soil Quality on Bamboo Growth 34
BE-35 The Effect of Soil on Growing Plants 35
BE-36 The Effects of Insecticidal Substances on Ants 36
BE-37 Body Color Change and Genetic Regionality of Japanese Red-Bellied Newt
(Cynops Pyrrhogaster) 37
BE-38 Producing Mineral Origin Pigments from Local Minerals 38

TABLE OF CONTENT
Page
CHEMISTRY
CR-01 Study on the Adsorption of Activated carbon from Pluang (Dipterocarpus
Tuberculatus Roxb.) by Chemical Activation with Phosphoric Acid (H3po4) 39
CR-02 Development On Ring-Opening Reactions of Aziridines in Green Solvents. 40
CR-03 Microwave-Assisted Rapid Synthesis of Tio2-Ag for Highly Photocatalytic Activity 41
CR-04 Product Development of Low Sodium Pickle Fish for Health 42
CR-05 Three-Dimensional Space Analysis of Cazymes Containing 5-Membered
Ring Sugar at Cleavage Site 43
CR-06 Structure and Biological Effects of Triglycerides Isolated
From Local Plant Extracts 44
CR-07 utility of organic acid on ring-opening of aziridine 45
CR-08 Preparation of Activated Carbon from Dipterocarpus Tuberculatus Roxb.
by Chemical Activation with Koh 46
CR-09 Silver Nanoparticles Synthesis Mediated by Plant Extracts 47
CR-10 Study of Solketal Ester Synthesis from Lauric Acid Catalyzed
by Potassium Hydroxide 48
CR-11 A Survey and Statistical Study of Cazymes that Contains Pyranose Sugar at
the Enzyme Active Site 49
CR-12 Virtual Screening and Molecular Docking of Natural Products
for Hepatic Anticancer Activity 50
CR-13 Antioxidant Activity of Crude Proteins and Protein Hydrolysate
from Lentinus Polychrous Lév. 51
CR-14 Antioxidant and Anti-Acetylcholinesterase Activities of Crude and Fractionated
Extracts from Curcuma Longa L. 52
CR-15 Four Types of Amino Acids: Comparison of Bacterial Growth 53
CR-16 creating colored dyes using purple cabbage and different PH solutions 54
CR-17 Study on the Production of Yogurt with Banana Pulp 55
CR-18 Making Soap from Horse Sweat 56

TABLE OF CONTENT
Page
CHEMISTRY
CR-19 Making Lip Balm using Flower Petals 57
CR-20 Finding the Facewash with The Most Moisture Content 58
CR-21 Comparison of Uv Protection Effects between Sunscreens and Makeup Bases
with Sunscreen 59
CR-22 Researching Different Properties of Plastics 60
PHYSICS AND ASTRONOMY
PA-01 Fabrication of Fluidic Chip Using a Laser Cutting Machine
for Spectrophotometry Analysis Application 61
PA-02 Vri Photometry and Light Curve Analysis of the W Uma-Type Binary
System Gv Leo 62
PA-03 Development and Optimization of an in-House Electrospinning Machine
for Polyvinyl Chloride Nanofibers Fabrication 63
PA-04 Synthesis of Composite Materials for a Radiative Cooling System 64
PA-05 The Relationship between Strength of Lead and Grade 65
PA-06 The Relationship between Temperature and Elastic Force 66
PA-07 Impact Changes Depending on the Type of Cushioning Material 67
PA-08 The Difference in Water Temperature at Thermal Equilibrium between
Different Types of Metals 68
PA-09 The Effect of Water Spraying on Asphalt Surface Temperatures 69
PA-10 How Elastic Couplers Affect the Acceleration of Objects Linked in a Chain 70
PA-11 Differences in Light Transmission by Color 71
PA-12 Checking the Strength of Hinoki (Cypress) Squared Lumber 72
PA-13 Heron’s Fountain 73
PA-14 Observing Paper Confetti Distribution Patterns 74
PA-15 The Relationship between Belt Friction and Weight 75
PA-16 The Relationship between Contact Surface Area and Frictional Force 76

TABLE OF CONTENT
Page
COMPUTER AND TECHNOLOGY
CT-01 Smart Air Purifier 77
CT-02 Mm Wave Radar Sensor for Healthcare RoboT 78
CT-03 The Aircraft Escort the Forest 79
CT-04 Development of Low-Cost Electric Bicycle with Gps Tracking
and Mobile Application Integrated 80
CT-05 Machine Learning for Image Based Species Identification of Culicoides
Biting Midges 81
CT-06 Developing a Head-Mounted Mixed Reality Device 82
CT-07 Improvement of Accuracy of Motion Tracking on Vr Using Ai Learning 83
MATHEMATICS AND STATISTICS
MS-01 A New Algorithm Used in Machine Learning to Screen Osteoporosis 84
MS-02 Characteristics and Volume of the Mathematical Bee’s Abdomen 85
MS-03 The Shortest Route without Motorways 86
MS-04 Finding the Most Effective Route Through Disneysea 87
MS-05 Differences in Convenience Store Products by Location 88
MS-06 Permutations of Vertices of Rotating Polygons 89
SOCIAL SCIENCE
SS-01 Medicine Label Dictionary 90

1
6th Japan-Thailand International High School Project Conference (2023) |
BIOLOGY AND ENVIRONMENT (BE)
EVALUATING THE POLYPHENOL CONTENT OF COFFEE
EXTRACTED USING HOT WATER VERSUS ETHANOL
Natthida Sunanta1
, Tanaporn Amjai1
, Wipasiri Soonthornchai1,2
, Payungsak Tantipaiboonwong3
,
Komsak Pintha3*
Abstract
The total extracted phenolic content in the coffee with hot water at 90 °C and with 95% ethanol at
room temperature for 12 hours were investigated by Folin-Ciocalteu assay. The results of this study have
shown that the aqueous extract with hot water [1 to 10 minutes] had higher total phenolic content than the
ethanolic extract. In addition, the coffee extracted with hot water for 3 and 4 minutes had higher total
phenolic content than other hot aqueous extracts. Determination of the total flavonoid content and
antioxidant activity test on the extract will be conducted in the next study.
Keywords: Total phenolic content, Folin-Ciocalteu assay, Coffee, Hot water extract
1Demonstration School University of Phayao, Phayao 56000, Thailand
2
School of Science, University of Phayao, Phayao 56000, Thailand
3School of Medicine Sciences, University of Phayao, Phayao 56000, Thailand
*Email: komsakjo@gmail.com
Natthida Sunanata andTanaporn Amjaiequally to this work

2
COMPARISON OF POLYPHENOL CONTENT OF GREEN TEA
EXTRACTED BY HOT WATER AND ETHANOL
Benyada Sudsai1
, Sirirat Moonkate1
, Wipasiri Soonthorncha1,2
, Komsak Pintha3
,
Payungsak Tantipaiboonwong3*
Abstract
Green tea is a popular drink enjoyed for its taste and the effects it provides. It helps in weight
reduction, lowers blood sugar levels, reduces cholesterol, and possesses anticancer and antioxidant
properties that combat free radicals. This research aims to compare the total phenolic content of green tea
extracted with hot water at 90 °C for 1, 2, 3, 4, 5, and 10 minutes and with 95% ethanol using the Folin-
Ciocalteu method. The study found that green tea extracted with hot water had a higher total phenolic
content than extraction with 95% ethanol, with the highest content observed in green tea extracted for 5
minutes. Further studies should be performed to determine the total flavonoid content and antioxidant
activity of extracts.
Keywords: Greentea, Phenolic, Extraction, Antioxidants, Free radicals
1Demonstration School University of Phayao, Phayao 56000, Thailand
2
School of Science, University of Phayao, Phayao, 56000 Thailand
3
School of Medicine Sciences, University of Phayao, Phayao 56000, Thailand
*E-mail: payungsak.t@gmail.com
Benyada Sudsai and Sirirat Moonkate equally to this work

3
CREATING PHYTO STEM CELL GROWTH THROUGH TISSUE CULTURE
OF CARNIVOROUS PLANTS FOR FUTURE ADVANCEMENT
Jidapha Sae-lee1
, Sasiporn Chobjit1
, Sarunporn Sutthaluang1
, Hathairat Laksuk1,2
,
Chatchawal Wongchai1,2*
Abstract
Carnivorous plants play a role in plant - insect interaction and show high potential for medical use
as folk medicine and high value substances. Our study has included the propagating to produce a large
quantity of plants, utilizing protoplasts, and extracting secondary metabolites. Researchers are interested in
creating callus cultures from three carnivorous plants:Venus flytrap (Dionaea muscipula), sundew (Drosera
communis), and butterworth (Pinguicula sp.).This process begins by preparing a nutrient medium with a 1/2 MS
and 1.0 mg/L of BA growth regulator. The culture media was prepared with 100 plates and used to culture
plant organs with 300 pieces in Dionaea muscipula, Pinguicula sp. and 400 pieces for Drosera communis.
The results of growth and development are recorded continuously. The result shows that in weeks 10 and 12,
callus production was found. The result shows the survival rate of 92% and a callus occurrence rate of 92%
in Drosera communis, with an average callus diameter of 0.58 cm.In contrast, Dionaea muscipula exhibited
a survival rate of 50.56% and a callus occurrence rate of 51.51%, with an average callus diameter of 1.12 cm.
As for Pinguicula sp., it had a survival rate of 3.67%and did not undergo callus formation, but the calluses could
not be maintained due to subsequent growth, preventing callus preservation from this experiment.Moreover,
the calluses obtained from this experiment can be further utilized in various scientific endeavors in the future,
including research in other fields.This opens exciting possibilities for scientific exploration and innovation.
Keywords: Callus, Venus flytrap, Sundew, Butterworth, Nutrient medium
1Demonstration School University of Phayao, University of Phayao, Phayao 56000, Thailand
2
Department of Biology, School of Science, University of Phayao, Phayao 56000, Thailand
*E-mail: wongchaimee2110@gmail.com

4
SEACHING FOR THE STRATEGIES TO ENHANCE FLAVONOID
CONTENTS HOUTTUYNIA CORDATA (PLUKAOW)
Jinnapat Yawila1
, Natthawat Puinok1
, Chotika Thongphan1
, Siriwat Boonchaisri2*
Abstract
Houttuynia cordata (Plu Kaow) is a medicinal plant commonly found in the northern region of
Thailand. The leaves of H.cordata have been traditionally used as medicinal foods. Flavonoids were the
most abundant ingredients which form a major component display of a wide range in pharmacological
activities. This study aimed to rapid shoot multiplication and investigated the effect of concentrations of
sucrose used with culture medium on growth and the production of flavonoids in H. cordata. The highest
shoots regeneration (96%) and shoot number per explant (1.82 ± 0.56) were obtained on MS medium
containing 1.0 mg/L 6-Benzyladenine (BAP) and 0.5 mg/L 1-Naphthylacetic acid (NAA) after 8 weeks of
culture. Explants of in vitro-grown shoots were subcultured on MS medium containing sucrose different
concentrations 0, 5, 10, 20, 30, 40 and 50 g/L for 4 weeks. The results showed MS medium containing only
30 g/L sucrose was the most effective medium for inducing explants regenerate shoots (5.83±1.108 shoots
per explant)and total chlorophyll content (0.029 mg/g).Total phenolic content (TPC)was measured by Folin–
Ciocalteu reagent method. The total phenolic content in the leaves of H. cordata were cultured on MS
medium containing 20 - 50 g/L sucrose significantly better than that 0-10 g/L sucrose. Therefore, this study
provides a scientific basis for quality control that can be obtained in food development, pharmaceutical
applications, and agricultural.
Keyword: Houttuynia cordata, Phenolic content, Plant tissue culture, Sucrose
1
Demonstration School University of Phayao, Phayao 56000, Thailand
2
*E-mail:siriwat.bo@up.ac.th

5
SEED GERMINATION PATTERNS AND MICROPROPAGATION
OF PROSTHECHEA FRAGRANS IN VITRO
Kunthira Ainkham1
, Kessara Khamduangdee1
, Kedsinee Khamnonngiw1
,
Thanyaporn Tangjaroenchai1,2*
Abstract
In nature, orchids are plants that are difficult to propagate because their seeds are powdery in nature.
This is constrained by the nutrients needed for the growth of the seedlings, as orchid seeds don’t have stored
food within them. As a result, the seedlings cannot germinate on their own. This research aims to study the
nutritional formula that affects the growth of the Prosthechea fragrans orchid and to propagate it to increase
its quantity. This will be done by studying the germination of the seeds and using a synthetic nutrient
formula combined with growth regulators NAA and 2,4-D at concentrations of 0.5,1.0,1.5,2.0,2.5,5.0 mg/L
and then adjusting the pH to be in the range of 5.2-5.25, which is the pH at which enzymes function well.
The growth will be documented by measuring the height of the stem, the length of the leaves, and counting
the number of leaves. The expected benefits from this research are to know which nutritional formula the
Prosthechea fragrans orchid thrives best in and to gain basic information about this orchid species.
Keyword: Prosthechea fragrans, Micropropagation and Seed germination
1
2
*E-mail: thanyaporn.bo@up.ac.th

6
THE EFFECT OF MEDIA ON GROWTH OF DENDROBIUM
FRIEDERICKSIANUM RCHB.F.
Teeratum Kamma1
, Nateetip Kharnjai1
and Thanyaporn Tangjaroenchai1,2*
Abstract
The purpose of this research is to study the growth of Dendrobium friedericksianum Rchb.f. From
the seed germination period until it develops into protocorm by tissue culture technique. In this study, a
total of 9 treatments were studied VW, VW+Kinetin 0.5 ppm.,VW+Kinetin 1.0 ppm.,VW+Kinetin 1.5 ppm.,
VW+Kinetin 2.0 ppm., VW+TDZ 0.5 ppm., VW+TDZ 1.0 ppm., VW+TDZ 1.5 ppm. and VW+TDZ 2.0 ppm.
Then measured growth and seed germination stage, it was found that there were a total of 6 germination
stages for 42 weeks. There are Stage 0 : Mature seed, Stage 1 : No germination, swollen embryo, Stage 2 :
Continued embryo enlargement, Stage 3 : Appearance of promeristem showing a pointed vegetative, Stage
4 : Root and leaves begin to grow and Stage 5 : The leaves and root are clearly visible and are ready to
transfer to different media.And measured seedling height and number of leaves.To provide information for
future studies of orchids.
Keywords: Dendrobium friedericksianum Rchb.f., Tissue culture, Media
1
Demonstration School, University of Phayao, Phayao 56000, Thailand
2
Division of Biology School of Science, University of Phayao, Phayao 56000, Thailand
*E-mail: thanyaporn.bo@up.ac.th
Teeratum Kamma and Nateetip Kharnjai equally to this work

7
ANATOMICAL ANALYSIS OF THE VEGETATIVE PARTS OF
GARDENIA SOOTEPENSIS HUTCH. (RUBIACEAE)
Punyapat Phansuwan1
, Techakon Palidwanon1
, Kongsakdi Promtep1
,
Chayanan Jitmanee1
, Boonchuang Boonsuk2*
Abstract
This research investigates the anatomical characteristics of the stem branches, petioles, and lamina
of Kham Mok Luang (Gardenia sootepensis Hutch.) using the paraffin method. To make permanent slides,
samples were cut into 16-18 micrometer-thick slices with a rotary microtome, stained with Safranin O and
Fast Green FCF, and sealed with DPeX mounting medium. The plant tissues were then examined under a
light microscope on permanent slides, and the plant samples were photographed with a digital photography
kit. The results showed that the stem branches had circular boundaries. The cortex and pith are mostly
parenchyma tissue with scattered idioblasts, and fibers were discovered to be sparsely distributed in the cortex.
The vascular bundle of stem branch is circular, with the phloem surrounding the xylem.The petiole's cross-section
resembles a semicircle. The epidermis is composed of trichomes that are uniseriate. The epidermis is
composed of trichomes that are uniseriate.The epidermis is composed of trichomes that are uniseriate.The cortical
layer is made up of parenchyma, aerenchyma, and idioblasts. The primary vascular bundle of the petiole
resembles the branch of the stem.Small vascular bundles can also be found in rows within the cortex to the left
and right of the main vascular bundle, as well as on the pith.The midrib is broad and convex on the underside.
Its main vascular bundle resembles that of the petiole. The leaf surface is covered with uniseriate hairs on
both sides, particularly on the dense underside. Stomata are located on the lower surface, and the palisade
mesophyll is composed of two to three layers. The leaf margins are downwardly curved. The results of this
study provide those who are interested with basic information regarding the anatomy of Kham Mok Luang.
Keywords: Anatomy, paraffin method, Kham Mok Luang, plant tissue, University of Phayao
1
2
Program in Biology, School of Science, University of Phayao, Phayao 56000, Thailand
*E-mail: boonchuang.bo@up.ac.th

8
EFFECTS OF ARTIFICIAL HABITATS ON DENSITY AND
SPECIES OF AQUATIC INSECTS
Panisara Wisai 1
, Ploychompoo Laohapongsiri 1
, Manassanan Jongtama 1
and
Chayanan Jitmanee1,2*
Abstract
The water sources in Phayao Province found many species of aquatic insects.According to a literature review
on aquatic insects, each species can live in a variety of water sources and have a different habitat. Therefore,
aquatic insects can be used to evaluate water quality. This study aims to find types of aquatic insects that live in different
artificial habitats, characterize artificial habitats affect aquatic insects and assess the water quality through
aquatic insects. The samples were collected between August 2023 to January 2024. The study area is Maeyien
waterfall which is located near Doi Luang National Park.The head of this watercourse is an abundance of nature
and few disturbances which suitable for being as habitat of aquatic insects. The five sampling sites were chosen, and
3 replicates of sampler were collected from each site. Artificial habitats were made, three types are natural such
as rocks, branches and wood-leaves and two non-natural types are Hester-Dendytiles and bricks. The results were divided
into 2 parts, that are the water quality and aquatic insects. The result from August to September, aquatic insects
were analyzed with BMWPThai Score and ASPT and the water quality was assessed by measuring the following
physical and chemical parameters. Now, the effect of artificial habitat results was loss, because of natural disasters.
The aquatic insect diversity in natural habitat which collects to compare with artificial habitat was found 50 families
from 7 orders.The most abundant order is Ephemeroptera, subordinate with Diptera and Trichoptera.The number
in family of baetidae (Ephemeroptera) were found in enormous followed by heptageniidae (Ephemeroptera), caenidae
(Ephemeroptera) and Chironomidae (Diptera), respectively. The ASTP values from all study sites are relatively similar.
The water quality by using aquatic insects indicated that fair to good which was similar to water quality by measurement.
Keywords: aquatic insect, diversity of aquatic insects, artificial habitat, water quality, Maeyien waterfall
1
²Division of Energy and Environmental management, School of Energy and Environment, University of
Phayao, Phayao 56000, Thailand.
*E-mail: chayanan.ji@up.ac.th

9
EFFECT OF THE FERMETED ALMOND LEAVES SOLUTION ON
THE COLOR OF GOLDEN FISH
Poramuth Leesan1
, Pitchapa Khamsutta1
, Chanyanut Komput1
, Kriengkrai Seetapan2
,
Wipasiri Soonthornchai1,1,3*
Abstract
Nowadays, almond leaves (Terminalia catappa), containing tannins, are used in the cultivation of
ornamental fish to help adjust water quality, prevent disease in fish, and accelerate the color of Siamese
fighting fish. Therefore, this study aims to evaluate the fermented almond leaf solution to enhance the color
of goldfish. Initially, 10 g of almond leaves were fermented in 1.5 L of water; therefore, the concentration
of the fermented solution was 66.67 mg/L. The study was divided into 2 concentrations, including 10 and
20 % of the fermented solution, with concentrations of 6.67 mg/L and 13.3 mg/L, respectively. Six goldfish
were reared for 4 weeks by measuring the color of the fish every week. The color of the body and fin of the
fish was measured using a color chart.The color chart will represent the density as a level of 1-5, then record
the results to compare the color change.The results indicated that almond leaves could not help accelerating
color in goldfish after collecting the experimental results for 4 weeks.This experiment used a small number
of fish, which may cause the results to have a high standard deviation.Therefore, the result of the experiment
was not clearly different in the color of the fish. For further experiments, we suggest that the number of fish
used in the experiment be increased to obtain clearer results.
Key words: almond leaves, Terminalia catappa, color, goldfish
1
Demonstration school, University of Phayao 56000, Thailand
2
School of Agriculture and Natural Resources, University of Phayao 56000, Thailand
3
School of Science, University of Phayao 56000, Thailand
*E-mail: wipasiri.so@up.ac.th

10
BIOINFORMATICS IN PLANTS MEDICINE FOR ANTI-CANCER,
ANTI-VIRUS AND ANTI-ALLERGY
Phattaneekorn Rattanasaeng1
Khemjira Thianwan1
Pheechakanid Saraban1
Chatchawal Wongchai2*
Abstract
Gene study using bioinformatics in plant medicine.The aim is to discover the genetic code of medicinal
plants that produce biological compounds that fight cancer, allergies and viral diseases.In this study, we will use
information from the NCBI website. We will look for 5 substances that can fight against these diseases from
plants and search for plants that these substances exist, then find the accession number (accession number) and
the name of the gene present in plants from the UniProt website and then find the sequence result of each gene
that we found on the Nucleotide website.The results of the study were that cancer is Vinblastine found in 3 types
of plants, a total of 39 genes, Curcumin was found in 6 types of plants, a total of 23 genes, Podophyllotoxin was
found in 5 types of plants, a total of 44 genes, Cannabinoids were found in 1 type of plant, a total of 28 genes,
Taxane was found in 8 types of plants, a total of 17 genes. Viral diseases include Oleanolic acid was found in
5 types of plants with a total of 15 genes, Rosmarinic acid was found in 4 types of plants with a total of 9 genes,
Andrographolide was found in 1 type of plant with a total of 5 genes, Ursolic acid was found in 5 types of plants
with a total of 14 genes, Quercetin was found in 3 types of plants with a total of 14 genes.12 genes and allergies
include Tannin found in 2 types of plants, a total of 5 genes, Kaempferol found in 2 types of plants, a total of 7
genes, Quercetin found in 2 types of plants, a total of 9 genes, Bromelain found in 1 type of plants, a total of 1
gene, Lycopene found in plants. 2 types, a total of 6 genes. This study can further expand medicine in medicine
from medicinal plants in the future.This information can also be used to study the possibility of other plants that
may haveanti-cancer, anti-viral and anti-allergic properties.
Keywords: bioinformatics, accession number, genetic code
1Demonstration School, University of Phayao, Phayao 56000, Thailand
2
Division of Biology, School of Science, University of Phayao, Phayao 56000, Thailand
*E-mail: Chatchawal.wo@up.ac.th

11
MOLECULAR CHARACTERIZATION OF NOVEL TRANSFORMING
GROWTH FACTOR BETA 2 (TGF-β2) GENE IN
GLYPHOGLOSSUS MOLOSSUS
Jetnipit Wongwut1
, Nayardar Tirapun1
, Wipasiri Soonthornchai1,2*
Abstract
G. molossus, an amphibian, is currently hunted and highly consumed; therefore, the number of
them is declining rapidly. However, little information on the genetics of G. molossus is explored. For this
reason, we characterized the novel transforming growth factor beta 2 (TGF-β2) gene. The sequence was
obtained from RNA sequencing and analyzed. Our result showed that the cDNA of TGF-β2 has an ORF of
1248 bp encoding 415 amino acids. This gene represents a signal peptide and the TGFB domain. The
predicted amino-acid sequence has 91%, 82%, and 81% identity with amphibians (Engystomops pustulosus
and Eleutherodactylus coqui), chicken, and human, respectively.In addition, it has 95%similarity with both
amphibians.Furthermore, a phylogenetic tree demonstrated that TGF-β2 protein was clustered into a branch
classifying as amphibian, avian, and mammal. Our results suggest that G. molossus has an identity and
similarity with Eleutherodactylus coqui, Engystomops pustulosus, Quasipaa boulengeri, Rana temporaria,
and Hyla sarda, respectively. Our finding provides the basic information about TGF-β2 gene for further
examining its functions.
Keywords: G. molossus, transforming growth factor beta 2 (TGF-β2), amphibian
1
2School of Science, University of Phayao 56000, Thailand
*Email: wipasiri.so@up.ac.th

12
ALTERATION OF THE BACTERIAL COMMUNITY IN INFECTED
FIGHTING FISH (BETTA SPLENDENS)
Kritphat Burtyothee1
, Pisitpunya Pato1
, Siriphat Kaewpeng1
, Kriengkrai Seetapan3
,
Wipasiri Soonthornchai1,3*
Abstract
Fighting fish (Betta splendens) hold a prominent position in Thailand's thriving aquaculture
industry, given their economic significance and aesthetic appeal. However, fighting fish farming However,
in Thailand, fighting fish farms still encounter serious problems with the spread of disease caused by viral
and bacterial infections, especially in the digestive system. Therefore, this study focuses on the dysbiosis of
the bacterial community in the digestive system of Betta splendens.We compared the bacterial communities
between healthy and infected fish using Illumina MiSeq sequencing of the 16S rRNA gene. To confirm the
infected fish, we extract the DNA from the digestive system to detect the Mycobacterium and
Megalocytivirus infection. Our results demonstrated that there was a significant difference between these
bacteria and viruses in healthy and infected fish. We also found that the bacterial community in the infected
fish was significantly lower than that in the healthy fish. These findings hold significant implications, as
fish can be leveraged to enhance fish feed formulations, thereby promoting the growth of beneficial
microbiota, and effectively preventing infections and diseases in fighting fish.
Key words: Betta splendens, bacterial community, infection, Mycobacterium, Megalocytivirus
1
Demonstration school, University of Phayao 56000, Thailand
2
School of Agriculture and Natural Resources, University of Phayao 56000, Thailand
3
School of Science, University of Phayao 56000, Thailand
*E-mail: wipasiri.so@up.ac.th

13
SITUATIONAL ANXIETY OF SUCCESSFUL FUTSAL ATHLETES IN
THE 45TH DEMONSTRATION GAMES
Puttinon kaenmaihom1
,Fhawongglod suthammeng1
,Jinnapat suttaloung1, Suriyon luangtrongkit2*
Abstract
The purpose of this research was to study and compare the level of anxiety according to the situation
between the Competition round, qualifying round, quarter-final round semi-finals, and the finals of Futsal athletes
which was successful in the 45th Samakkhi Demonstration Competition.Sample group used in research He is a
male Futsal athlete who There were 14 people successful in the 45th Samakkhi Demonstration Competition.
The research instrument was a questionnaire. Situational Anxiety, Revised Edition. (Revised Competitive State
Anxiety Inventory -2 (CSAI2R)translated and edited by Phichit Muangnapho, consisting of 3 aspects.1)Physical
anxiety It has a precision of 0.75 and a reliability of 0.69.2)Mental anxiety has a precision of 0.85 and a reliability
of 0.67. 3) Self-confidence has a precision of 0.83 and a reliability of 0.76. Data were analyzed by finding the
percentage, mean (M), and standard deviation (S.D.). The results found that 1. Situational anxiety regarding
physical anxiety (Somatic Anxiety)of Futsal athletes who were successful in the unity demonstration competition
in the qualifying round Quarterfinals It is at a low level. The semi-finals are at a moderate level. And the finals
were at a high level.2.Situational anxiety Cognitive Anxiety of Futsal Athletes who were successful in the unity
demonstration competition in the qualifying round Quarterfinals The semi-finals and finals were moderate.
3. Situational anxiety Regarding anxiety and self-confidence (Self - Confidence) of Futsal athletes who were
successful in the unity demonstration competition in the qualifying round. The quarterfinals, semifinals,
championship and final rounds were moderate.
Keywords: anxiety, futsal athletes, anxiety levels, competition rounds
1
2
School of Health and Physical Education, University of Phayao, Phayao 56000, Thailand
*E-mail: Suriyon.koe@gmail.com

14
ANTICANCER EFFECTS OF HARRISONIA PERFORATA (BLANCO) MERR.,
STREBLUS ASPER LOUR. AND ARECA CATECHU L. ETHANOLIC
EXTRACTS ON HUMAN ORAL CANCER CELL LINE HSC-7
Natchapol Polket1
, Torpong Boonyawiwat1
, Irawadee Jandarat1
, Thipphiya Karirat2
, Vijitra Luang-in2*
Abstract
Oral cancer is the sixth most common cancer in Thailand and more prevalent in smokers than non-smokers.
From the past to the present, three herbal plants, namely gotha wood (Harrisonia perforata(Blanco)Merr.), khoi twig
(Streblus asper Lour.) and betel nut (Areca catechu L.) have been commonly utilized for consumption and usage in
certain localities in Thailand.However, a clear comparison of the anti-oral cancer activity of these three plant extracts
is yet to be reported.Therefore, the aims of this work were to study the anticancer activity and antioxidant activity of
three herbal plant ethanolic extracts against human oral cancer cell line, HSC-7.The results showed that the Ferric ion
Reducing Antioxidant Power (FRAP) antioxidant activity was highest in gotha wood (3.56±0.05 mg Fe(II)/g DW),
whilst the least was in betel nut (0.63±0.10 mg Fe(II)/g DW). As for the anti-oral cancer activity using 3-[4,5-
dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide(MTT)method at 24 h, the IC50 values of gotha wood, khoi twig
and betel nut were in the following order:40.21±0.30, 47.60±0.45 and 258.37±5.56 µg/mL, respectively.The highest %
cytotoxicity of three herbal extracts at 800 µg/mL was 89%, 84% and 63%, respectively. Colony formation assay
indicating long-term antiproliferative activity showed that the gotha wood extract had the best effect (IC50 =29.11±0.86
µg/ml). Likewise, the gotha wood extract showed the most obvious apoptotic induction of cancer cells which started
to appear at the lowest concentration.Wound healing assay also showed that the gotha wood extract exhibited the most
effective anti-migratory effect against HSC-7 cells. These three herbal plant extracts displayed strong-to-very weak
anticancer activities against HSC-7 cells in the following order:gotha wood, khoi twig and betel nut, respectively.The
gotha wood and khoi twig extracts can be applied for oral care product development with anti-oral cancer benefits.
Keywords: Cytotoxicity, Areca catechu, Harrisonia perforata, Streblus asper, Oral cancer cell HSC-7
1
Mahasarakham University Demonstration School (Secondary), Maha Sarakham, 44150, Thailand.
2
Natural Antioxidant Innovation Research Unit, Department of Biotechnology, Faculty of Technology,
Mahasarakham University,Maha Sarakham, 44150, Thailand.
*E-mail: vijitra.l@msu.ac.th

15
PREPARATION OF KOMBUCHA SYNBIOTIC GUMMY FROM GREEN
TEA INFUSED SHATAVARI TEA PLUS INULIN FROM YAM BEAN
Kanyakorn Tonongwa1
, Thunrada Chantanam1
, Pattarapapa Tayommai1
, Woranan Nakbanpote2*
Abstract
Kombucha is a beverage by the fermentation process of symbiotic culture of bacteria and yeast
(SCOBY).The combination of probiotics and prebiotics is known as synbiotics.Green tea is a popular tea among
young people. Shatavari (Asparagus racemosus Willd.) is a medicinal plant that supports health and boosts
immunity.Yam bean (Pachyrhizus erosus)is a good source of inulin and fiber, which are prebiotic.Shatavari and
Yam bean are native plants to Maha Sarakham Province. The aim of this research is to make synbiotic using
kombucha that has been fermented from sweet green tea infused Shatavari tea, and inulin extracted from yam
beans is used as a prebiotic.The fermentation results of sweet green tea and sweet green tea plus Shatavari were
compared.The monitoring for 25 days of fermentation indicated that SCOBY in the system with Shatavari was
thicker.However, the pH values of both fermentations gradually decreased from 3.2 to 2.7 during 25 days. The
total phenolic content (TPC) and total flavonoid content (TFC) values did not significantly differ between the
systems with and without Shatavari.TPC and TFC in the green-tea kombucha were 332.64 ± 11.81 mg GAE/L
and 65.86±6.18 mg EPE/L, respectively. Whereas, TPC and TFC in the kombucha obtained from green-tea
infused Shatavari were 317.63±34.09 mg GAE/L and 76.65 ±16.30 mg EPE/L, respectively. The DPPH (2,2-
diphenyl-1-picryl-hydrazyl-hydrate) scavenging activities indicated that IC50 values of 0.16 mM DPPH for the
green tea kombucha, the green tea-infused Shatavari and ascorbic acid were 2.1 g/L, 2.0 g/L and 0.05 mg/L,
respectively. In addition, the taste of kombucha complements the inulin extracted from yam bean. Therefore,
synbiotic gummy bears from kombucha that contain antioxidants and probiotics, and a prebiotic supplement
from inulin make them suitable as a functional food.
Keywords: Antioxidant; inulin; kombucha; Shatavari; symbiotic
1
Mahasarakham University Demonstration School (Secondary), Maha Sarakham, 44150, Thailand
2
Department of Biology, Faculty of Science, Mahasarakham University, Maha Sarakham, 44150, Thailand
*E-mail: corresponding_woranan.n@msu.ac.th

16
METHOD VALIDATION FOR MICROPLASTIC EXTRACTION FROM
NORTHEASTERN THAILAND FERMENTED FISH SAUCE BY
HYDROGEN PEROXIDE AND TEMPERATURE
Chonthicha Samohaeng1
, Yanida Dermtumrum1*
, Onjira Petchwichit1, Akeapot Srifa2
Abstract
Although the harmful effect of consumption and accumulation of microplastics is still unclear in
human and animal health, microplastics have been detected everywhere, even in aquatic organisms and in
food. Recently, microplastics were detected in the digestive tract of northeastern Thai local fish used to
make fermented fish sauce (FFS). The focus of this study was on the validation of methods used to extract
microplastics from local commercial FFS in northeastern Thailand, where conventional methods used to
extract microplastics from animal digestive tissues might not be effective. A commercial brand of FFS was
used to extract microplastic at two temperature (25°C and 65°C)and four extracting agent conditions (10:10,
10:15, 10:20 and 10:25 ml/ml of FFS: 30% H2O2). Clarity of the extract was observed as a measure for the
effectiveness of the extraction process. It was found that the extraction rate at 65°C was faster than that at
25°C, and that the extraction rate was directly related to the amount of hydrogen peroxide used in the
reaction. The best extraction rate was at 10:25>10:20>10:15>10:10 ml/ml of FFS: 30% H2O2, respectively.
After the reaction, the extracts were filtered by a nylon mesh to observe microplastics under stereo
microspoce, where some shapes and colors of microplastics were found. It is noteworthy that the extracts
were often viscous, probably due to organic matters, and mesh size of nylon played a major role in the rate
of filtration. Small mesh would take a very long time to filter but retain most extracted microplastics while
using large mesh allows quick filtration but most microplastic may be lost. This might further affect the
quantitative analysis of microplastic abundance and prevalence in samples.
Keywords: Fermented fish sauce, microplastics, hydrogen peroxide, temperature, extraction
1
2
Mahasarakham University, Department of Biology, Faculty of science, Mahasarakham, 44150, Thailand
*E-mail: 6540003@msu.ac.th

17
SCREENING OF EXTRACELLULAR L-ASPARAGENASE PRODUCING
BACTERIA FROM RHIZOSPHERIC SOIL AND ROOTS
OF EUPHORBIA HIRTA L.
Chonruedee Yotasri1
, Sasinan Saenwang1
, Sutammanit Sompak1
, Rungruedee Thiwthong2*
Abstract
This study aimed at isolating and screening of extracellular L-asparaginase producing bacteria from
rhizospheric soil, rhizosplane and roots of Euphorbia hirta L. Modified M9 medium containing 0.5 g/L. L-
asparagine with phenol red as indicator was used for bacterial isolation. The total of 10 bacterial isolates
obtained from rhizospheric soil (1 isolate), rhizosplane (2 isolates) and roots (7 isolates) were subjected to
investigating growth and extracellular L-asparagenase production under shaking condition.Among them, 2
isolates (RPEh01 and RPEh02)from rhizoplane were found to produce high L-asparaginase with the specific
activity of 0.822 and 0.408 Unit/mg Protein respectively. The results of the 16s rRNA gene sequencing
revealed that RPEh01 and RPEh02 were closely to Bacillus megateriumand Pseudomonas plecoglossicida
(RPEh02), respectively.
Keywords: extracellular L-asparaginase, rhizoplane bacteria, 16s rRNA gene
1
Mahasarakham University Demonstration School (Secondary), Maha Sarakham 44150 Thailand
2
Department of Biology, Faculty of Science, Mahasarakham University, Maha Sarakham 44150 Thailand
*E-mail: rungruedee.t@msu.ac.th

18
USING HERBAL DETERGENTS FOR DUST MITE ELIMINATION
Chaninart Chinabutr1
, Thanyaphon Laoluecha1
, Mingkamol Kaeworasan1
, Nakorn Pradit2*
Abstract
Dust mites are the major cause of allergiesin humans.Two species of dust mite, Dermatophagoides
pteronyssinusand Dermatophagoides farinae are commonly found in the dust from the mattresses, carpets,
or fabric objects. Regularly washing is the simplest method to control dust mites. However, using chemical
detergents may irritate the skin cause allergic reactions to the detergents. In this study, four herbs; Makhamdi
Kwai(Sapindus rarak), Mee(Litsea glutinosa (Lour.)C.B.Robinson.), Hnam Thaeng(Catunaregam tomentosa
(Blume ex DC.)Triveng.), and Kheru Khao Kham (Cuscuta reflexa Roxb)will be examined on the possibility
to control the dust mites as herbal detergents. First, the dust mites were explored for mass rearing. Of
11 room samples, only 3 room samples have dust mites. The room with the highest dust mite population has high
humidity, high temperature, and less cleaning habits. The dust mites from the exploration were grown in
the plastic cup and fed with finely ground yeast. These plastic cups were placed in a box filled with
concentrated NaCl to prevent the growth of yeast hyphae.Each herb will be extracted according to the local
wisdom by crumpling specific part of each plant in the water. For testing the effectiveness of herbal detergents.
The survival of dust mites will be recorded. We expected that the herbal detergents from this study will be
a new option for people who are allergic to chemical detergents for controlling the dust mite in their houses.
Keywords: Dust mite, Allergy, Detergents, Herbal
1
Mahasarakham University Demonstration School(Secondary), Maha Sarakham, 44150, Thailand.
2
Walairukkhavei Botanical Research Institute, Mahasarakham University, Maha Sarakham, 44150, Thailand.
*E-mail: Nakorn.p@msu.ac.th

19
FILM FORMING SPRAY OF CISSAMPELOS PAREIRA EXTRACT AND
PIPER SARMENTOSUM EXTRACT AGAINST BACTERIA
Kullapas Dolrung1
, Chayanit Kaewsuto1
, Matina Saengsawat1
, Catheleeya Mekjaraskul2*
Abstract
Infection on the skin surface often involves bacteria, with Staphylococcus bacteria being a prominent
causative agent. The current treatment approach employing antibacterial faces challenges due to increasing
antibacterial resistance.As a result, researchers have sought new alternatives and discovered that several Thai herbs
possess antibacterial properties, especially the leave of Cissampelos pareira and Piper sarmentosum, both of with
are indigenous plants commonly found in Thailand.These herbs exhibit significant antibacterial effects against the
Staphylococcus group.Therefore, the objective of this study was to investigate the antibacterial properties and to
develop a film forming spray formulation using C.pareira and P.samentosumextracts.The leaves of C.pareira were
boiled in water for extraction, while theP.samentosumleaves were extracted using 95%ethanol.Subsequently, these
extracts were tested for their antibacterial properties against S.aureus and S.epidermidis using the micro broth
dilution.The film forming sprays were then developed by using various amounts of cosolvents and film forming
agents. The result revealed that the combination of P.samentosum and C.pareira leaf extracts exhibited better
antibacterial properties against S.aureus and S.epidermidis compared to the extract from each C.pareira. and
P.samentosum. Therefore, the combination of P.samentosum and C.pareira leaf extracts was selected for the
development of film forming spray.The optimized spray formulation utilized various solvents, with a mixture of
PEG 400 (0.2%), water (15%) and ethanol (adjust to 100%) as the sovent system. The addition of HPMC (3%) and
biosaccharide gum-1 (1%), film forming agents, yielded the most favorable formulation, resulting in a green solution
with a pH of 7.19 ± 0.05 and a film thickness of 0.01 mm.The developed film demonstrated flexibility, cohesion
and adhesion, making it suitable for various applications.
Keywords: Film-forming spray, Film-forming agent, Antibacterial activity,Cissampelospareira,Pipersamentosum
1
Mahasarakham University Demonstration School (Secondary), Maha Sarakham, 44150, Thailand.
2Faculty of Pharmacy, Mahasarakham University, Maha Sarakham, 44150, Thailand
*E-mail: catheleeya.m@msu.ac.th

20
A STUDY ON THE RESPONSE OF TRIBOLIUM CASTANEUM
TO ULTRASONIC WAVES
Chayamat Changthongkam¹, Preeyaphorn Kamlangyong¹, Atikun Meechantee¹, Nattawoot Suwannata²*
Abstract
This study investigates the impact of ultrasonic waves on the mobility of the Red flour beetle
(Tribolium Castaneum) within Thailand's jasmine rice (Khao Dok Mali 105 variety). The research involves
the observation of Red flour beetles in response to both audible and ultrasonic frequencies spanning from
5 kHz to 80 kHz. To conduct this experiment, a power amplifier is utilized to magnify the small sine wave
signal generated by a function generator. The amplified signal is subsequently applied to an ultrasonic
transducer within the voltage range of 5 to 45 Vp-p. The experimental findings reveal that some of the Red
flour beetles exhibit displacement from the transducer at specific frequency intervals. These movement
patterns are particularly prominent within the audible frequency range of 5 kHz to 20 kHz when the
transducer voltage operates within the range of 30 Vp-p to 35 Vp-p. Additionally, movement responses are
observed in the ultrasonic frequency range of 35 kHz to 55 kHz when the transducer operates at a voltage
of 20 Vp-p.
Keyword : Insect, Red flour beetle, Tribolium Castaneum, Ultrasonic waves
¹Mahasarakham University Demonstation School (Secondary), Maha Sarakham, 44150, Thailand
²Faculty of Engineering, Mahasarakham University, Maha Sarakham, 44150, Thailand
*E-mail: 6210094@msu.ac.th

21
DEVELOPMENT OF SEMI-CROSS FLOW BIOFILTER SYSTEM FOR
WATER FILTRATION IN ORNAMENTAL FISH AQUARIUM
Warakorn Chankue1
, Korawit Sengsod2
, Suphada Kiriratnikom2*
Abstract
Currently, the breeding of ornamental fish in Thailand has provide income opportunities for fish farmers.
However, many species of ornamental fish are sensitive to water conditions. Therefore, the filtration system is
crucial in controlling various factors within the water, the conventional aquarium system which act as a dead-
end filtrating operation need to clean filter media regularly To reduce clogs of impurities on the filter, which
wastes time and cumbersome for fish farmers while cross flowfiltration is more efficient in filtering and reducing
clogs on the filter media .So the experiment was undertaken by compared removal efficiency of nitrogen water
turbidity and pH between the semi cross flow filtration (SCF) prototype and convention aquarium filtration
system (dead-end filtrating operation, DE). Five replications of each experimental aquarium filtration system
were equipped in 36-liter glass aquarium which raising 6 goldfish in each aquarium. Fish in each experiment
system was fed with ornamental fish feed twice time daily. Water sample were collected for analysis of water
quality every 5 days for 2 months period.The results showed SCF has advantages more efficient for ammonia
removal than DE, on day-45, 55 and 60 it was found that ammonia concentration was 1.11,1.52 and 1.95 mg/l
respectively in SCF which significantly lower (p<0.05) than 0.37,0.52 and 0.16 mg/L respectively in DE.
Furthermore, SCF also reduces the turbidity on day-30 0.53 NTU compared to 0.98 NTU for the DE.Moreover,
it can control the pH value to stay within the standard range is more effective than 6.5-9.0.All of this demonstrates
that SCF has the capability of efficient filter cleaning.The IoT segment controlling aquarium SCF system with
a real-time remote-control application will be developed for further study.
Key words : Semi cross flow filtration (SCF) , Dead-end filtration (DE) , Ornamental fish , Self-cleaning
1
Paphayompittayakom School (SCiUS TSU), Paphayom District, Phatthalung 93210, Thailand
2
Department of biological science and environment, Faculty of science and digital innovation, Thaksin
University, Phatthalung campus 93210, Thailand
*E-mail: ksuphada@gmail.com

22
FOUR TYPES OF AMINO ACIDS: COMPARISON
OF BACTERIAL GROWTH
Daiki Iwasaki1
Abstract
Amino acids are often used in bait because of their ability to attract fish, but they can also cause
the proliferation of bacteria in the environment where fish are kept and thus cause the water quality in the
aquarium to decay. The four main amino acids that fish are attracted to are arginine, glycine, alanine, and
glutamine. In this experiment I investigated the effect of different amino acids on the growth of bacteria.
Five petri dishes with the 4 different amino acid solutions and one with no added amino acids were prepared
and left to incubate for 2 days. I then recorded the number of colonies that had grown. This process was
repeated 3 times and the average values used for comparison.Out of the four tested amino acids, glutamine
had the highest rate of bacterial colony growth, more than 2 times that of the petri dish with no amino acid.
Conversely, arginine had an inhibitory effect on the growth of bacteria, thought to be due to the alkalinity
of the arginine solution.
1
Tokai University Takanawadai Senior High School, Tokyo, Japan

23
INVESTIGATING THE RELATIONSHIP BETWEEN KILLIFISH
GROWTH AND PROTEIN SOLUTIONS
Hirotaka Ishitawa1
Abstract
My experiment was to give four kinds of amino acids to medaka to investigate changes in growth.
Knowing how different amino acids affect the growth of fish could help the aquaculture industry to produce
medaka more efficiently. The experimental tools were aquariums, medaka, medaka food, four amino acids,
(arginine, alanine, glutamine, and glycine), a ruler, a beaker, and an electronic balance. I prepared 5
aquariums, one for each of the amino acids and a control with no added amino acid solution.The aquariums
were prepared by dissolving 1g of the amino acids in each tank.Three medaka were placed in each aquarium
and fed the same amount and at the same time. The length and weight of the medaka were measured once
a week for four weeks. Glycine showed the greatest increase in medaka growth rate, most likely due to the
positive effect that glycine has on the quality and amount of sleep of the medaka.
1

24
POPULATION CHANGES OF EUGLENA IN DIFFERENT
ENVIRONMENTS
Komei Nishizaki1
Abstract
Recently, biomass fuels have been produced using euglena, a single celled micro-algae with
features of both animal and plant lifeforms. A large amount of Euglena is required for biomass fuel
production. The purpose of this experiment was to examine how euglena growth is affected by the type of
colored light. The experimental method was to prepare 10 ㎖ of Euglena culture solution, add a growth
hormone, and leave it for one week exposed to either red, green, blue or white light. After a week Euglena
growth was examined and compared between the groups.White light was found to have significantly higher
Euglena growth compared to the other types of light. Green light resulted in the lowest growth rate, most
likely due to the relative inefficiency of that wavelength in photosynthesis.
References
Euglena Propagation and Photosynthesis - Efficient Propagation Method, Photosynthesis and pH
Kanazawa Izumigaoka High School
Lee Unno, Yuka Nakayama, Shugo Kawakami, Seiya Sunayama, Yuta Wakabayashi
Culture experiment of Paramecium vol.2
1
Tokai University Takanawadai senior High School

25
GROWTH RATES OF MEDAKA
Ryota Nagashima1
Abstract
The killifish is our easiest fish to keep and our most familiar creature. I became curious and
wondered to what extent killifish, which live in polluted ponds and rivers, could withstand changes in their
environment. My hypothesis was that the growth of killifish would vary depending on the aqueous solution
in which they live, so I conducted an experiment to see how different pollutants would affect the growth of
killifish. In this experiment I investigated glycine, India ink, salt, and glucose. These pollutants were
dissolved into the killifish’s tanks up to a concentration of 2%.The weight of the killifish was then measured
after one month. I found that the killifish in the saltwater solution had the highest weight after one month.
Interestingly, the more difficult the environment in which the killifish lived, the higher their growth rate
was. This may be because killifish respond to their environment by enlarging the organs that circulate
respiration and other organs.
1

26
RELATIONSHIP BETWEEN THE PRESENCE OF
WOOD CHIPS AND SOIL BIOTA
Saito Ozawa1
, Hayate Sato1
Abstract
We are interested in finding soil that is good for growing plants. Organisms that live in the soil are
necessary to improve the soil environment. In this experiment we studied the effect of covering soil with
woodchips on the number of organisms in the soil. In our experiment, we used a Tullgren funnel to collect
soil organisms. We then compared the soil organisms in the soil under woodchips and in soil without any
covering. We poured 500 ml of soil into the Tullgren funnel for 4 hours. During November, December, and
April, we examined the type and number of soil organisms and summarized them on a graph. The soil
moisture content (%) was then measured by first drying the soil at 120°C for 2 days and then calculating the
weight change before and after drying. In November, December, and April, the number of collembolan and
ticks was high under woodchips. This was likely due to moisture content and temperature being maintained
under woodchips.Under woodchips the number of soil organisms remains high even in cold weather, which
may promote decomposition of organic matter and maintain good soil quality.
References
1. https://www.i-field.jp/tullgren/
2. https://ikimono-fun.com/post-274/
3. http://www.edu.pref.kagoshima.jp/curriculum/rika/chuu/sentakurika/seibutu/kensaku.pdf
1

27
COMPARISON OF COLONY GROWTH WITH DIFFERENT KINDS
OF DRINKS
Takeshi Tanaka1
, Kenshi Moriuchi1
Abstract
Beverages play a crucial role in our daily lives, but they can also become a breeding ground for
microorganisms. The objective of this experiment is to uncover the influence of different beverages on the
growth rate of microorganisms, providing information to make healthier and more hygienic beverage
choices. To assess the bacterial growth in different beverages, a small amount of each of the drinks was
placed in sterile petri dishes. Each set of petri dishes were divided into 3 temperature groups of 3°C, 27°C,
and 37°C. Progress was observed once a week and the number of colonies was counted. In this experiment,
the higher the temperature, the greater the number of colonies, and the degree of increase was completely
different depending on the beverage. Milk and orange juice had the highest rate of bacterial growth at all
temperatures while water had the lowest.In conclusion this experiment showed that water is the safest drink
out of those tested.
1Tokai University Takanawadai Senior High School, Tokyo, Japan

28
SOUTHERN POND SHRIMP AND FOOD TYPES
Yutaro Uchiki1
Abstract
My experiment is to see if the growth rate and number of eggs of southern pond shrimp changes
depending on the type of food we feed them. This experiment will make it easier to keep and breed the
shrimp, which are in need as pets and fishing bait. This experiment studied 30 southern shrimp divided into
three groups who were fed frozen spinach, redworms, and commercial shrimp food respectively. Shrimp
were carefully dried and weighed once a week for 7 weeks and the average weight for each group of shrimps
recorded. Shrimp that died and those that had spawned were removed from the experiment. Frozen spinach
was found to be the most beneficial food source with shrimp in that group having the lowest decrease in
weight across the 7 weeks and resulting in the greatest number of spawnings. Conversely, commercial
shrimp food was the least beneficial with no spawnings during the 7 weeks.
1

29
THE RELATIONSHIP BETWEEN LIGHT COLOR AND GROWTH OF
SHIITAKE MUSHROOMS
Haruto Izumi1
, Naonori Kirishiki1
, Haruto Samizo1
Abstract
We studied the effect of light colour on the growth of shiitake mushrooms. A Japanese research
institute studied the relationship between shiitake and light and found that blue light was the best. The
purpose of our research is to investigate how shiitake growth changes when grown in different colour lights.
In this study, the colours of light compared were blue, red, green, and no light. The shiitake were grown
over one week, at 18o
C, and 60% or higher humidity. The number of shiitake grown, and their length and
width were measured. The results showed that blue light produced the highest number of shiitake, red light
produced the longest shiitake, and blue and green lights produced the widest shiitake. The no light shiitake
were the worst in all categories. In the future, we would like to understand and study the effects of light
intensity and light colours.

30
FINDING SUBSTANCES THAT INHIBIT YEAST GROWTH
Hikaru Umegai1
Abstract
Many countries in Asia have a very hot climate, such as, Thailand, Vietnam and Japan. Food in
these countries can spoil and be dangerous due to an increase in bacteria. Bacteria can also be found on a
variety of surfaces. I wanted to find a substance that inhibits the growth of fungi. I wanted to learn how to
kill the bacteria around me. In particular, I wanted to find spices that have antibacterial effects. At the very
least, I hoped that I wouldn’t get sick when eating food and be less likely to catch a cold. I tested various
substances in an agar medium, such as capsaicin; citric acid; vitamin C and catechins. There was a wide
range in the number of bacteria colonies, from none to more than 1,000.The citric acid and catechin samples
showed almost no evidence of yeast infection.The citric acid was sterilized by oxidative action, and catechin
destroyed cell membranes, which probably led to sterilization.Catechins destroyed cell membranes, leading
to sterilization. Therefore, both are considered to have a bactericidal effect. Of the four substances used in
my experiment, catechin and citric acid had the most antibacterial properties.Finding the appropriate degree
of dilution was difficult since it was very difficult to count the number of colonies. Also, it was also
challenging to find the concentration of each substance at which the growth of yeast fungi is suppressed.
For this experiment, I found that it is possible to sterilize with acidic substances. I will now look to see if
alkaline substances are also effective in sterilization.I will also conduct a comparison experiment next time
to see whether acidic or alkaline substances are more effective in sterilization. I will also conduct an
experiment to compare which is more effective in sterilizing catechins and citric acid.
1

31
WATER AND MICROORGANISMS
Kanta Hirabayashi1
Abstract
I did an experiment on the relationship between water and microorganisms because I was interested
in the conditions under which microorganisms in the water increase and decrease. Three aquariums, A, B,
and C, were set up with air pumps. Two killifish and gravel were placed in tank A, two killifish and three
water plants in tank B, and two killifish by themselves in tank C for one week. Second, an absorption
photometer was used to measure the number of microorganisms. In addition, agar medium and pack tests
were conducted. The results showed that the microorganisms in A and B increased but decreased in C.
Nutrient agar C had the most bacteria, but A’s bacteria did not increase. The pack test showed that B had
the highest oxygen concentration. It was thought that the microorganisms escaped predation by killifish by
living in obstacles such as gravel and aquatic plants.
References
光合成解析センター生物試料の分光測定
生物試料の分光測定 (photosynthesis.jp)
株式会社島津製作所
UV TALK LETTER vol.2 分光光度計の構造 : 株式会社島津製作所 (shimadzu.co.jp)
1

32
REGENERATION RATE OF PLANARIAN FLATWORMS DUE TO
CHANGES IN WATER QUALITY
Kaon Ishii1
Abstract
I conducted this experiment because I was interested in the fast regenerative abilities of planarians.
In this experiment, I investigated the effects of acidic, alkaline, and neutral water on the regeneration rate
of planarian flatworms. Vinegar was added to water to produce acidic water and bleach was added to water
to produce alkaline water. The pH of the waters was measured using pH test strips. After separating the
planaria into three containers each with a different pH of water, the planaria were all cut in half, to
investigate how fast they regenerated over a three-week period.Planarians are covered in mucous that makes
them highly alkaline.Therefore, it was assumed that their regenerative ability would be fastest in an alkaline
environment. The results of the experiment after the three-week period showed that the planarians
regenerated the most in alkaline water. Neutral tap water showed the second highest regeneration rate and
acidic water showed little regeneration. The average regenerations were 0.35mm, 0.32mm and 0.28mm
respectively.
References
1. https://www.ritsumei.ac.jp/tanq
2. https://saiseiiryo.jp/skip_archive/knowledge/basic/planaria
3. http://www.cdb.riken.jp/jp/millennium/2_1.html
1

33
GROWTH OF KILLIFISH IN DIFFERENT ENVIRONMENTS
Kodai Watanabe1
Abstract
River water, pond water, and rice field water are common types of freshwaters in Japan.These three
environments are clearly different.For this research, I wanted to find how these environments affect killifish
growth so we can raise killifish easily. To find the best environments, I measured the height and weight of
the killifish, the amount of biomass in their environments and the water quality. Killifish were raised in
three different aquarium environments using river, pond, and rice field water. A killifish was put into each
environment and fed for two weeks. Then, four kinds of pack tests were used to test water quality, PO4
3-
,
NH4
+
, NO2
-
, COD.Also, the killifish length and weight were measured through observation and using digital
scales once a week. The results showed there were no changes in weight or length of the killifish. The
amount of biomass was not different between the three environments, but there were differences in the pack
test. Especially, pond water had the highest NH4
+
and PO4
3-
and rice field water had the highest NO2
-
.
In conclusion, pond water is the best for killifish.From the NH₄ results, pond water had the most excrement
in the water. This is because the killifish ate a lot of food, so they produced more excrement. The results of
the PO₄ and NO₂ tests showed that there was a lot of live biomasses in the pond and there was less dead
biomass in the pond water. Therefore, pond water is the best for raising killifish.
References
1. Piyush Yadav. November 11, 2023.
2. Jun Mori, Masakazu Mizutani, and Junzi Takahashi. April 10, 2008.
1

34
THE EFFECTS OF WEATHER AND SOIL QUALITY
ON BAMBOO GROWTH
Kokoa Oku1
, Masahiro Tsunemi1
Abstract
The landscape and climatein Japan are ideal for the growth of many types of bamboo, making it possible
to find bamboo scattered throughout every region.It is a highly renewable and sustainable resource.The diversity
of bamboo species varies depending on different factors such as soil quality. This means that bamboo can be
used as indicators of different forest conditions. We wanted to investigate the relationship between soil quality
and bamboo growth. Our objective was to study the effects of weather and soil quality on bamboo growth. We
studied the weather conditions, soil quality, and the bamboo growth rate at various sites. We collected
temperature and precipitation data from July to September 2022 at Sagamiko Lake. For bamboo growth, we
measured the circumference, height, and the surface temperature of the bamboo.For soil quality, we measured
organic matters, moisture, surface temperature, the temperature inside the soil, pH and elements. We found that
there was more growth in July than in August.Our results showed that Site 8 had the best soil quality, Site 9 had
average soil quality and Site 10 had the worst soil quality. We also found that Site 10 had the highest average
temperature, Site 9 had median average temperatures and Site 8 had the lowest average temperatures.We found
that the ideal conditions for growing bamboo are low temperatures with low precipitation.As for soil, we found
that higher soil temperature, lower soil quality, and neutral pH are the best for growing bamboo. In the future,
we would like to measure the bamboo from shoots, so that we can obtain more accurate results. We also plan to
measure the circumference at predetermined heights to obtain more accurate results. Finally, we would like to
investigate how sunlight affects bamboo growth.
References
1. Japan Meteorological Agency Meteorological Data of Lake Sagami
2. Masahiro Ikeda "Writing Materials and Climate Change" Kyoto Sangyo University Journal of
Humanities Volume 50 p. 207-230 Kyoto Sangyo University
1

35
THE EFFECT OF SOIL ON GROWING PLANTS
Ryutaro Fujii1
Abstract
Akadama soil is said to be the best for plants, but I did not know whether this was true. I wanted to
find out so that I would be able to grow plants more effectively. This experiment tested what soil has the
best effect on daikon and arugula. A mixture of six kinds of basic soil (akadama, kanuma, kawasuna,
yamasuna, tatsuchi, and kurotsuchi) and culture soil, and culture soil by itself were tested. Three arugula
seeds were grown in plastic cups of each type of soil for 12 days, and this was repeated for daikon. The
stems were measured and recorded each day, and on the last day, the daikon were weighed. Akadama soil
produced the longest arugula, but culture soil produced the shortest and the heaviest daikon. So, the most
effective soil for each plant was different.
References
1. Tetsuro Kato. Basis of soil and manure and how to make and use them by illustration. Natsume
company, 2017.
2. https://www.atariya.net/
3. http://www.yuri-h.akita-pref.ed.jp/yuri-h-cms/assets/uploads/2022/03/2906-tuti.pdf
4. https://www.sc-engei.co.jp/gardeningbeginner/basics.

36
THE EFFECTS OF INSECTICIDAL SUBSTANCES ON ANTS
Shuntaro Tani1
Abstract
Environmental issues and the use of chemicals have become a hot topic in recent years. I have had
an interest in ants since elementary school. Ants are omnivorous and interact with many organisms, placing
them at the top of the ecosystem.Therefore, there is concern that a decrease in the number of ants will affect
the lower levels of the ecosystem. So, I wanted to create an environmentally friendly insect repellent
specifically for ants. These experiments tested soil, sulfur, naphthalene and methanol as ant insecticides by
placing them in a box with the ants and timing how long it took the ants to arrive at the bait with the
insecticidal substances. The results of the experiment showed that the ants only arrived at the bait for soil
and sulfur. However, the ants preferred the soil the best.The sulfur was not very effective in killing the ants.
Naphthalene and methanol were found to be highly toxic to ants and therefore were determined to be
effective insect repellents.
1

37
BODY COLOR CHANGE AND GENETIC REGIONALITY
OF JAPANESE RED-BELLIED NEWT (CYNOPS PYRRHOGASTER)
Fujimoto Hana1
, Nishimura Marika1
Abstract
Japanese red –bellied newts (Cynops pyrrhogaster) are capable of changing their body color,
however the specific conditions required for this change to occur remain unclear. In this study, we sought
to explore the mechanisms underlying the regional variation in the ease of body color change not related to
sexual dimorphism. Newts were exposed to various conditions for 48 hours and their back color was
measured using a color reader. DNA analysis was also done on newts from three different locations using
their tail tips. From the DNA analysis results obtained so far, it was not possible to conclude whether there
was a regional characteristic responsible for the color change.
1
Hyogo Prefectural TOYOOKA Senior High School, Hyogo Pre., Japan

38
PRODUCING MINERAL ORIGIN PIGMENTS
FROM LOCAL MINERALS
Tsuchida Mio1
, Nawa Yuka1
Abstract
We went down to the riverside of the Sagae River and collected some minerals; mudstones, tuff and
green tuff, and tried making pigments. By pulverizing the minerals until they became a fine powder, and
mixing the powder with gelatinous adhesive with water, we succeeded in making 4 colors of pigments, but
the colors were not vibrant. In the following experiment, we tried to stain agate taken from Mt. Gassan in
order to produce a blue pigment, by using cobalt chloride and ammonia. Due to agate’s cryptocrystalline
structure, we were able to stain some parts of agates, but thicker solutions and thinner slices of agate seems
necessary.
1
Yamagata Prefectural Touohgakkan Jr. & Sr. Highschool, Yamagata Pre., Japan

39
CHEMISTRY (CR)
STUDY ON THE ADSORPTION OF ACTIVATED CARBON
FROM PLUANG (DIPTEROCARPUS TUBERCULATUS
ROXB.) BY CHEMICAL ACTIVATION
WITH PHOSPHORIC ACID (H3PO4)
Chanidapa Naksawad1
, Kanasiri Kaenjhan1
, Chanisa Luecha1
, Supachai Wanprakhon1,2*
Abstract
Activated carbon is an excellent adsorbent that can adsorb a wide range of organic and inorganic
substances due to its highly porous structure.It can be produced from all carbon-containing materials, which
are produced from raw materials that contain carbon as the main constituent. In this research, we decided to
use Pluang (Dipterovarpus tuberculatus Roxb.) as biomass materials, which are readily available locally.
Making activated carbon requires a four-step process, of which the initial step is burning to obtain carbon
or charcoal.The second step is calculating carbon in the charcoal state.The third step is activating the carbon
surface to form a porous or hollow structure with phosphoric acid (H3PO4), and the last step is characterized
using the iodine number test method.
Keywords: activated carbon, phosphoric acid, Pluang, charcoal
1
2 School of Science, University of Phayao, Phayao 56000, Thailand
*E-mail: supachaixxxwanprakhon@gmail.com

40
CHEMISTRY (CR)
DEVELOPMENT ON RING-OPENING REACTIONS OF AZIRIDINES
IN GREEN SOLVENTS.
Natchayapat Dararattikan1
, Thipthanya Kasorn2
, Lalita Radtanajiravong1,2
*
Abstract
The aim of this project green solvents; Deep Eutectic Solvent (DES), is to find a suitable for the
ring opening of aziridines to produce chloro-compound as a final product.DES is lower in evaporation rate,
less inflammation and toxic more temperature resistance than the typical organic solvents; therefore, it is a
potential and environmentally friendly solvent.The various DES solvents were prepared by mixing the two
or three components at 80°C for 2 hours. Two DES solvents which are choline chloride:aspirin and choline
chloride:levulinic acid were prepared in this project. Then the obtained DES was used in the reaction with
the chosen aziridine.After the work-up process, TLC was used to detect the formation of products in crude
mixture. The mixture was isolated by column chromatography in the well-developed eluent system. 77% of
the desired ring-opening product was obtained from the column. Then NMR analysis of the product
substances was conducted to analyze the chemical structure of the synthesised compounds. As a result of
analysis, choline chloride:levulinic acid DES might be a potential solvent for the ring opening of aziridine,
however, further Optimisation studies are needed in the future.
Keywords: opening aziridines, Deep eutectic solvent (DES), purity, choline chloride
1
Demonstration School, University of Phayao, Phayao, Thailand 56000
2
Department of Chemistry, Faculty of Science, University of Phayao, Phayao, Thailand 56000
*E-mail: lalita.ra@up.ac.th

41
CHEMISTRY (CR)
MICROWAVE-ASSISTED RAPID SYNTHESIS OF TIO2-AG FOR
HIGHLY PHOTOCATALYTIC ACTIVITY
Phirapat Wongma1a
, Natthatida Sila1a, Sutthasinee Katunyoo1,2*
Abstract
Titanium dioxide (TiO2)is a compound that naturally occurred when titanium reacts with the oxygen
in the air. Due to its properties, TiO2 has been found for many different environmentally applications. As a
photocatalyst, TiO2 can be added to paints, cements, windows, and tiles to decompose environmental
pollutants. Silver nanoparticles (AgNPs) are one of the most vital and fascinating nanoparticles among
several metallic nanoparticles. AgNPs include catalytic activity, optical, and antibacterial properties. In the
current work, we discuss the synthesis of TiO2-Ag compound using microwave-assisted method in 100 W
for 30, 45, and 75 cycles in order, in 300 W for 30, 45, and 75 cycles in order.We also discuss the properties
of TiO2-Ag compound for their characterization.Moreover, we extensively discuss the applications of TiO2-
Ag compound for photocatalytic activity.
Keyword: Microwave-assisted, Titanium dioxide, Silver, Photocatalytic activity.
1
Demonstration school of University of Phayao, University of Phayao, Phayao, 56000, Thailand
2
Department of Chemistry, School of Science, University of Phayao, Phayao, 56000, Thailand
a
Authors contributed equally to the work.
*E-mail: Suttasinee.ka@up.ac.th

42
CHEMISTRY (CR)
PRODUCT DEVELOPMENT OF LOW SODIUM
PICKLE FISH FOR HEALTH
Kunakorn Katsri1*
, Phachara Suthammeng2
, Siwakorn Ketanawa2
,
Yutthasat Punkeaw2
and Pisarn Khuaelit2
Abstract
Pla-som is a traditional fermented fish or pickle fish from Thailand. It is commonly used in the
preparation of Sodium chloride, a traditional source of salted taste. This study aimed to: (1) investigate the
amount ofSodium chloride and Potassium chloride ratio (100:0, 75:25, 50:50, 25:75 and 0:100)to the suitable
formula of Pla-som during the fermentation for 3 days and (2) analyze the physical and chemical properties
such as color (L*, a*, b*), pH, texture profile and sensory acceptance of pickle fish prepared from Sodium
chloride and Potassium chloride for different level. The color (L*, a*, b*) of 5 treatments were not different
during the change of the ratio of Sodium chloride and Potassium chloride. The lightness (L*) of Pla-som
treatment 3 (75.34) was brighter than other treatments. While the pH found that, according to its pH (4.22–
4.41), Pla-som is considered as safety food product following Thai Industrial Standards Institute
recommended during 4.00-6.00. Texture profile took place during fermentation after 3 days, as shown by
the increase amount of Potassium chloride. Such processes contributed to the hardness development in Pla-
som. Not only the springiness and cohesiveness of Pla-som was not different during the fermentation, but
also resilience too. In addition, Gumminess and chewiness was higher dependent on Potassium chloride
from 990.31 to 1819.44 (gumminess) and 897.68 to 1552.46 (chewiness). However, the sensory testing
revealed consumer preference for Pla-som was evaluated. The appearance, color, odor, taste, and texture of
5 treatments were not different. Treatment 2 was higher score (7.19) in overall liking than other treatment.
Keywords: Traditional Food, Pickle Fish, Low Sodium, Sodium Choline, Potassium Chloride
1
School of Agriculture and Natural Resources, University of Phayao, Phayao, 56000, Thailand
2
Demonstration School, University of Phayao, Phayao, 56000, Thailand
*
E-mail: k.katsri@gmail.com

43
CHEMISTRY (CR)
THREE-DIMENSIONAL SPACE ANALYSIS OF CAZYMES
CONTAINING 5-MEMBERED RING SUGAR
AT CLEAVAGE SITE
Benyapa Kansutham1
, Pranpreeya Uparakul1
, Wijitra Meelua1,2
, Jitrayut Jitonnom2*
Abstract
CAZymes are the carbohydrate- active enzymes and associated carbohydrate- binding modules
involved in the synthesis and degradation of complex carbohydrates. The purpose of this research was to
explore and study the data on furan sugars in the CAZyme catalytic region and to analyze the statistical
values of the data obtained from the CAZy database.The data that was analyzed consisted of types of furan
sugar molecules, proportions, and types of amino acids that act as catalysts (acid/base and nucleophile), the
orientation of the sugar structure relative to the two catalysts, as well as the sugar conformation at the
catalytic site. This data will be helpful for a better understanding of the mechanism of action of enzymes
that can be applied in further studies.
Keywords: CAZymes, Glycoside Hydrolase, Protein-carbohydate interaction, Data analytics
1
2
*
E-mail: jitrayut.018@gmail.com

44
CHEMISTRY (CR)
STRUCTURE AND BIOLOGICAL EFFECTS OF TRIGLYCERIDES
ISOLATED FROM LOCAL PLANT EXTRACTS
Runthidapa Singchai1
, Pitak Nasomjai2
Abstract
Triglycerides, also known as triacylglycerols, are lipids composed of three unstable molecules of
fatty acids along with one molecule of glycerol, linked by ester bonds. When all three fatty acid molecules
(R1, R2, R3) in a triglyceride molecule are the same, it is referred to as a "simple triglyceride."If they differ,
it is called a " mixed triglyceride." Triglycerides have various applications, including their use in the
production of biodiesel through synthesis with other substances. This project aims to isolate triglycerides
from local plants of the Santalales order.It is based on the understanding that triglycerides can be found in
these plant species.The process involves solvent extraction, TLC (Thin-Layer Chromatography)analysis to
identify the desired compounds, column chromatography using silica gel as the stationary phase and a
mixture of hexane and ethyl acetate as the mobile phase, and further purification to obtain pure compounds.
TLC is employed again to confirm the purity of the final compounds. The structure and the type of fatty
acids are determined using analytical techniques such as GC-MS, NMR, and IR .
Key words: Unsaturated Triglycerides, Column chromatography, Non-polar solvent, Santalales
1
2
*
E-mail: pitak.na@up.ac.th

45
CHEMISTRY (CR)
UTILITY OF ORGANIC ACID ON RING-OPENING OF AZIRIDINE
Thanapat Ainmueangkaew1
, Thanison Maichom1
and Lalita Rattanajirawong1,2
Abstract
Deep Eutectic Solvents (DESs) are solutions made from mixing organic base and organic brønsted
acid, resulting in a eutectic solution with the properties of a green solvent that can adjust its physical and
chemical properties.It has the potential to act as an effective solution for solubilizing and extracting a wide
range of substances, and for transformation in organic reactions such as ring-opening reactions of aziridines.
To produce products used in the chemical industry. This DES method is environmentally friendly and low
in toxicity. In this research, the aim is to study organic acids for use as a solvent in the aziridines ring
opening reaction to study the suitable components in DES that have the greatest effect on the synthesis,
Moreover, DES was used to extract and study the composition of important substances in plants such as
Andrographis paniculata. DES was synthesized and then utilized in the extraction, The reaction process
obtained from the ring opening reaction was and plant extraction then tested using Thin Layer
Chromatography (TLC) and Nuclear magnetic resonance (NMR) methods. In the ring-opening reaction it
was found that the result of DES containing 1 : 2 choline chloride and lactic acid and product yield is 27%
by ‘H NMR. DES choline chloride and Levulinic acid 1 : 2 can be extracted and Andrographolide can be
detected by TLC.
Keywords: Deep eutectic solvents, Andrographolide, Aziridine, Ring-opening, Organic acid
1
2School of Science, University of Phayao, Phayao 56000, Thailand
*
E-mail: lalita.ra@up.ac.th

46
CHEMISTRY (CR)
PREPARATION OF ACTIVATED CARBON FROM DIPTEROCARPUS
TUBERCULATUS ROXB. BY CHEMICAL
ACTIVATION WITH KOH
Thanchanok Maharattanaviroj 1
, Jirawat Pinrat 1
, and Supachai Wanprakhon 1,2
Abstract
Activated carbon is carbon that has a high adsorption ability due to its extensive surface area and
numerous pores. These are keys in absorbing various molecules. Activated carbon can be produced from
carbon-rich material. in this research, we are interested in producing activated carbon by using Pluang
(Dipterocarpus Tuberculatus Roxb.) seeds. Since the Pluang tree is commonly found in Thailand, the seeds
of the Pluang tree have no other practical use apart from being used for planting. Therefore, this research
aimed to study the preparation of activated carbon from Pluang (Dipterocarpus Tuberculatus Roxb.) by
chemical activation with KOH. The experimental procedure involves subjecting Pluang to a carbonization
process at a temperature of 500 degrees Celsius for 1 hour to increase carbon content. Activate the carbon
with KOH in the concentrate of 85% for 2 hours to increase the pore volume and surface area and then heat
it to the desired temperatures for study: 500, 600, 700, and 800 degrees Celsius. Determine the iodine
number to examine the efficiency of adsorption.
Keywords: Activated carbon, Potassium hydroxide, Chemical activation, Dipterocarpus Tuberculatus
Roxb.
1
Demonstration School University of Phayao, Phayao Thailand 56000
2 School of Science, University of Phayao, Phayao, Thailand 56000
*Email:supachaixxxwanprakhon@gmail.com

47
CHEMISTRY (CR)
SILVER NANOPARTICLES SYNTHESIS MEDIATED BY
PLANT EXTRACTS
Warunya Marasean1a
, Warisa Manitham1a
and Pitak Nasomjai1,2*
Abstract
The synthesis of silver nanoparticles can be done in a variety of ways, such as microwave
production, and laser ablation process. Those processes are not environmentally friendly methods.
Therefore, the aim of this research is to synthesize silver nanoparticles from plant extracts of the genus
Sterculia monosperma (SM) using biosynthetic techniques, in different concentrations, to compare
nanosilver particle sizes. Characterization of the synthesized silver nanoparticles was done by using UV-
vis spectroscopy, FT-IR spectroscopy, and Transmission Electron Microscopy (TEM). The optimum
condition for synthesizing silver nanoparticles was as follows, plant extract concentration was 0.2 %wt/v,
silver nitrate concentration was 4.6x10-4
M, kept the mixture in the dark overnight. The result showed
maximum absorbance at 434 nm which corresponded to the formation of silver nanoparticles.
Keyword: Silver nanoparticles, Synthesis, Plant extracts
1
Demonstration school of University of Phayao, University of Phayao, Phayao, 56000, Thailand
2Department of Chemistry, School of Science, University of Phayao, Phayao, 56000, Thailand
a
Authors contributed equally to the work.
*
E-mail address: pitak.na@up.ac.th

48
CHEMISTRY (CR)
STUDY OF SOLKETAL ESTER SYNTHESIS FROM LAURIC ACID
CATALYZED BY POTASSIUM HYDROXIDE
Putthawong T.1
, Phetburi D.1
, Nasomjai P.2
Abstract
This research aimed to increase the versatile use of glycerol, a byproduct from biodiesel synthesis.
Glycerol is typically used in the energy and cosmetics industries, among others. Therefore, the research
team seeks to explore ways to diversify.This research focuses on its use in a medical way. This study has 2
parts; the first part is converting glycerol into solketal and the second part is converting a solketal via
esterification reactions with lauric acid known for its antibacterial and antiviral properties. The goal is to
obtain a solketal ester with pharmacological properties. The products obtained can be analyzed using thin-
layer chromatography (TLC) and Fourier-transform infrared spectroscopy (FT-IR). In this study, Catalyzed
by Potassium Hydroxide using a solketal to lauric acid mole ratio of 1:1; the temperature at 70°C; reaction
time of 60 minutes.
Keywords : Solketal Ester, Fatty acids, Biodiesel, Esterification Reaction, Glycerol
1Demonstration School, University of Phayao, Phayao 56000, Thailand
2School of Science, University of Phayao, Phayao 56000, Thailand
*E-mail: 65341787@up.ac.th

49
CHEMISTRY (CR)
A SURVEY AND STATISTICAL STUDY OF CAZYMES THAT
CONTAINS PYRANOSE SUGAR AT
THE ENZYME ACTIVE SITE
Pawanpat Vijitprapasin1
, Pranida Chucham1
, Jitrayut Jitonnom2
, Wijitra Meelua1,2*
Abstract
The CAZy database now has a vast amount of data on the 3D structure of carbohydrate-active
enzymes (CAZymes), but using and visualizing them is a challenging undertaking, making it impossible for
any users or scientists to grasp the structure and function of the CAZymes. We conducted a statistical
analysis as well as a data survey of different facts on the pyranose ring and enzyme at the active site in this
work. The CAZy database to be analyzed contains about thousand of 3D structure of protein-carbohydrate
complex from 183 GHs family. Several metrics were retrieved and analyzed, including Cremer-Pople
analysis on sugar conformation at the scissile location, important amino acid types ( acid/ base and
nucleophile at the active site), and peptide sequence analysis. Overall, we present helpful information that
advances our current knowledge of CAZyme activity.
Keywords: CAZymes, Cremer-Pople, Glycoside hydrolases, Protein carbohydrate interaction, Data
analytics
1
2
*
E-mail: wijitra.me@gmail.com

50
CHEMISTRY (CR)
VIRTUAL SCREENING AND MOLECULAR DOCKING OF NATURAL
PRODUCTS FOR HEPATIC ANTICANCER ACTIVITY
Jiratchaya thanarungrueangloed1
, Thanatpan Pharanat1
, Sornsawan Merkkhuang1
, Nadtanet Nunthaboot2*
Abstract
Hepatocellular carcinoma (HCC) is the fourth leading cause of death globally, and the survival rate
for patients is declining. The treatment landscape for this type of cancer remains challenging for scientists.
In this research endeavor, the focus was on studying compounds within the group of natural products found
in Thai herbs. The objective was to screen for natural product compounds that hold potential for inhibiting
key proteins associated with liver cancer, including Unc-51 Like Autophagy Activating Kinase 1 (ULK1),
mammalian Target of Rapamycin (mTOR), and Histone deacetylase (HDAC). Various computational
approaches including drug likeness screening, ligand based virtual screening and molecular docking were
performed.The results obtained from this computational chemistry method could serve as foundational data
for the development of highly effective agents in the treatment of hepatocellular carcinoma.
Keywords: Hepatocellular carcinoma, Unc-51 Like Autophagy Activating Kinase 1 (ULK1), mammalian
Target of Rapamycin (mTOR), Histone deacetylase (HDAC), Virtual screening, Molecular docking
1
Mahasakham University Demonstration School(Secondary),Mahasarakham,44150,Thailand.
2
Multidisplinary Research Unit of Pure and Applied Chemistry and Supramolecular Chemistry Research Unit,
Department of Chemistry and Center of Excellence for Innovation in Chemistry PERCH-CIC, Faculty of
Science, Mahasarakham University, Maha Sarakham, 44150, Thailand
*
E-mail: nadtanet.n@msu.ac.th

51
CHEMISTRY (CR)
ANTIOXIDANT ACTIVITY OF CRUDE PROTEINS AND PROTEIN
HYDROLYSATE FROM LENTINUS POLYCHROUS LÉV.
Canaan Maksawat1
, Nuntawadee Sangsikaew1
, Phattarathida Wongsatep1
, Rakrudee Sarnthima2
Abstract
This research aims to study the antioxidant properties of protein extract and protein hydrolysate
from the Lentinus polychrous Lév, by extracting fresh mushroom using distilled water. The protein content
was measured using the Bradford method, revealing a protein content of 0.893 milligrams per milliliter in
the extract. After freeze-drying, the extract content was found to be 15.04 milligrams per milliliter. The
extract was then tested for its ability to eliminate free radicals using the ABTS and DPPH assays, resulting
in 93.68% ABTS radical inhibition (IC50 value of 0.038 milligrams per milliliter) and 42.21% DPPH radical
inhibition (IC50 value of 0.051 milligrams per milliliter). The protein extract was further enzymatically
hydrolyzed using pepsin for 15, 30, and 60 minutes, followed by testing its radical inhibitory effects,
showing ABTS inhibition of 5.82%, 3.50%, and -0.54%, and DPPH inhibition of 34.73%, 35.64%, and 34.89%
at 15, 30, and 60 minutes respectively.This study demonstrates that the antioxidant properties of the protein
extract and protein hydrolysate from the Lentinus polychrous Lév. are more effective at eliminating free
radicals than the protein hydrolysate prepared with pepsin.
Keywords : ABTS, Antioxidant, DPPH, Lentinus polychrous Lév, Protein hydrolysates
1
2
Mahasarakham University, Department of Chemistry, Faculty of science, Mahasarakham, 44150, Thailand
Email: ungingnuntawadee@gmail.com

52
CHEMISTRY (CR)
ANTIOXIDANT AND ANTI-ACETYLCHOLINESTERASE ACTIVITIES OF
CRUDE AND FRACTIONATED EXTRACTS FROM CURCUMA LONGA L.
Tussavee Jongnui1
, Than Bunmanee1
, Panita Kongsun2
and Netnapa Chana2
*
Abstract
The population of people aged 60 years old and above is expected to increase. Alzheimer’s disease (AD) is
the most common in the aged population. AD is caused by several factors such as reduced neurotransmitter
acetylcholine and oxidative stress. The currently approved drug, an Acetylcholinesterase (AChE) inhibitor, enhances
the patient’s memory and cognition. However, it is accompanied by severe side effects. Hence, natural products are
being explored as an alternative to AD treatment because they have a variety of properties and biological activities. In
the present study, the crude ethanolic extraction, and isolation of crude alkaloid and non-alkaloid component were
obtained from turmeric (rhizome of Curcuma longa L.). The phytochemical evaluation of the turmeric extract was
performed using thin-layer chromatography (TLC), revealing bands of curcuminoids in the crude ethanolic extract.
The UV-visible absorption spectrumindicated a maximum absorption peak at a wavelength of 425 nm, attributed
to curcumin. All extracts were assessed for their anti-AChE and antioxidant activities. The results demonstrated that
the non-alkaloid fraction exhibitedthe highest AChE inhibition, with an IC50 value of 18.45±1.36 µg/mL.Conversely,
the ethanolic and alkaloid fractions showed IC50 values of 36.35±1.65 µg/mL and 57.96±2.31 µg/mL, respectively.
Notably, the non-alkaloid fraction demonstrated AChE inhibitory activity close to Galantamine (IC50 value was
13.62±9.22µg/mL).TheassessmentofDPPHradicalscavengingrevealedthatallturmericextractalsohasgoodantioxidant
activity with an IC50 value ranging from 8.83-62.46 µg/mL. Specifically, the non-alkaloid extract exhibited a DPPH
radical scavenging activity of 45.61 ± 2.61 µg/ml, while the alkaloid and ethanolic extract showed values of 8.83 ± 2.60
µg/mL and 62.46 ± 2.39 µg/mL,respectively. In conclusion, this study highlights the therapeutic potential ofthe non-
alkaloid fractionfromturmericasanaturalagentfortreatingADandotherneurologicaldisorderstriggeredbyfreeradicals.
Keyword: Alzheimer's disease, turmeric extract, non- alkaloid fraction, alkaloid fraction, antioxidant,
antiacetylcholinesterase
1
Paphayompittayakom school, Thaksin university
2
Department of Chemistry, Faculty of Science, Thaksin University, Phatthalung, Thailand
*Email: : netnapa@tsu.ac.th

Abstract_Japan-Thai Confernces 2.pdf

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