Understanding Molecular Biology With Techniques and Applications | The Lifesc...
Sandhya Gangaraju
1. Sandhya Gangaraju
Phoenix, AZ • 480 696 0417 • gsraju25@gmail.com
Sandhya Gangaraju Page 1
CAREER PROFILE
Principal research specialist in biochemistry and cell biology, compliance officer, and laboratory manager.
Research specialist responsibilities include experimental design, personally implementing and performing
research techniques, leading data interpretation, analysis, and presentation of experimental findings and
supervising student and staff researchers as they conduct experiments. Compliance officer responsibilities
include monitoring and maintaining the staff’s adherence to lab safety standards. This involves remaining
updatedon allintricaciesof laboratory safetythrough regular trainingseminars and consistentlyexecutingthese
safety standards both through instruction and example. Lab manager duties include receiving, training and
supervising incoming students and staff members.
SKILLS
● Cell culture
● Metabolic analysis of single cells
● Development of new methodologies
● Clinical Research
● Enzyme immunoassays
● Western Blotting
● Proper handling of clinical specimens
● Exposure to Mass spectrometry
● SDS PAGE
● Fluorescence Microscopy
● Immunostaining
● Cell Viability Assays
● RNA Extraction
● DNA Purification
● Polymerase chain reaction
● Immunohistochemistry
● Flow Cytometry
● Adenovirus purification
● Trans well Assays
● Lab Institutional Biosafety Committee
● Lab compliance
EDUCATION
Master of Science in Biochemistry/Cell Biology 2003
University of Ottawa, Canada, Department of Biochemistry, Microbiology and Immunology
Thesis: Role of mitofusin 2 in the regulation of Mitochondrial Dynamics
Master in Science in Biochemistry 1997
Kasturba Medical College (MAHE), India
2. Sandhya Gangaraju
Phoenix, AZ • 480 696 0417 • gsraju25@gmail.com
Sandhya Gangaraju Page 2
Bachelor in Science in Chemistry and Biology 1994
Sri Venkateswara University, India
AWARDS
Ontario graduate scholarship in Science and Technology September 2000- August 2002
Admission Scholarship – University of Ottawa September 2000-August 2002
Serving University Needs Award for positive attitude and continuous efforts at improving processes at
CBDA, Arizona State University Jan 2015
PROFESSIONAL EXPERIENCE
The Biodesign Institute at Arizona State University March 2014- present
Center for Biosignatures Discovery Automation
Principal Research Specialist and Compliance Officer
Manage cell culture facility by providing technical cell culture support, supervision and training of
incoming cell culture technicians, oversee adherence to biosafety and standard operating procedures
within the facility.
Specific bench work involvespropagationof cells, establishmentofprimary culturesfrom human tissue
samples, maintenance of 3D organoids from mice small intestine, cell clusters, and understanding of
metabolic properties of single cells.
Lead experimental design for students and junior staff members, perform and manage existing
experiments, make major contributions to the interpretation, reporting and presentation of project
results.
Train and supervise graduate students and junior staff in both the technicalities of conducting research
and the standards necessary to maintain a safe laboratory environment.
3. Sandhya Gangaraju
Phoenix, AZ • 480 696 0417 • gsraju25@gmail.com
Sandhya Gangaraju Page 3
Optimization of working protocols for novel microfluidic devices in effort to adapt these protocols to
serve the purpose of studying three-dimensional tissue microenvironments established by Barrett’s cell
lines.
Managed a clinical research project by collaborating with Mayo clinic which involved proper handling
of clinical specimens, preparing primary cultures from tissue samples, documentation of results and
reporting progress on a regular basis and ensuring the adherence to policies of Institutional Review
Board.
National Research Council, Ottawa, Canada Sep 00 – Mar 13
University of Ottawa Heart Institute, Ottawa, Canada
Technical Officer - Experimental Stroke Group (Apr 09 – Mar 13)
Management of viral facility (BSL 3)
● Maintained BSL 3 viral facility, including general maintenance and operating procedures, supervised
and trained lab personnel in proper techniques for virus production, purification and concentration.
Differential expression of Robo1 (Roundabout axon guidance receptor 1) following ischemia and
long-term recovery
● Aided in examining a possible protective role of Robo1 in reducing vascular permeability following
cerebral ischemia, which involved culturing Human Umbilical Vein Endothelial Cells (HUVECs) as the
in vitro model system
● Utilized western blotting to detect the levels of Robo1 in mouse brain tissues; immunohistochemistry
and fluorescence microscopy to understand localization of Robo1 in mouse brain tissue and HUVECs.
● Developedtranswellassaysto study the role of Robo1 in neutrophil transmigration through endothelial
cells.
Investigating the role of ceacam6 in pancreatic cancer using Ceacam6 single domain antibodies
● Measured binding efficiency of GFP (Green Fluorescent protein) virus to Ceacam6 in human
embryonic kidney cells through flow cytometry followed by statistical analysis by Graph Pad Prism
Identification of lipid raft-mediated signaling pathways following stroke in a mouse model
● Assisted with membrane fractionation, western blotting, immunostaining and microscopy to elucidate
the membrane dependent and independent signaling pathways in mouse model of cerebral ischemia
4. Sandhya Gangaraju
Phoenix, AZ • 480 696 0417 • gsraju25@gmail.com
Sandhya Gangaraju Page 4
Technical Officer - Neurogenesis and Brain Repair Group Apr 06 – Mar 09
Development and implementation of lentiviral technology to deliver neurotropic factors to neural
cells
● Assisted with astrocyte mediated glial derived neurotrophic factor (GDNF) delivery in dopaminergic
neuronal protection
● Maintained, propagated and differentiated Human NT2 (teratocarcinoma cell line) and rodent
primary cultures
● Generated undifferentiated NT2 cells stably expressing GDNF using lentiviral technology
● Conducted quantitative analysis of GDNF using ELISA to detect presence of recombinant GDNF
● Assessed cell survival and proliferation on neuroimplants using immunostaining and TUNEL assays
● Examined motor cortex of injured mouse before and after receiving neuroimplants
Role of Sox-2 in the proliferation and differentiation of human amniotic fluid cells
● Accountable for maintenance, propagation and generation of human amniotic cells transduced with
Sox-2.
Role of BMP7 in neuronal survival and differentiation
● Determined level of BMP7 in neural progenitors, neurons and astrocytes by ELISA
● Investigated differentiation of neural progenitors in the absence or presence of BMP7 utilizing
immunocytochemistry
Role of bFGF 2 in neuroprotection
● Studied the role of bFGF2 on NMDA-induced toxicity in cultured cortical neurons using cell viability
assays (MTT- (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide)
● Responsible for delivering data on-time; presented seminars and lab meetings to provide progress
updates of projects.
Neuroprotective effects of novel water-soluble formulations of antioxidants in MPTP model
● Performed morphometric analysis of Tyrosine hydroxylase neurons in substantia niagra region of the
mouse brain via immunohistochemical staining
5. Sandhya Gangaraju
Phoenix, AZ • 480 696 0417 • gsraju25@gmail.com
Sandhya Gangaraju Page 5
Technical Officer - Neurogenesis and Brain Repair Group Mar 04 – Mar 06
Investigated role of preconditioning in mitochondrial biogenesis
● Standardized and implemented methods for labeling mitochondrial DNA; assessed and standardized
subtoxic doses of different methods of neuronal preconditioning in order to analyze changes in
mitochondrial biogenesis.
University of Ottawa Heart Institute, Ottawa, ON Canada Sep 00 – May 03
Graduate Student
Project focusing on Mitochondrial Dynamics
● Investigated molecular mechanisms by which Mitofusin regulates mitochondrial fusion in mammalian
cells, includingmicroscopicanalysisofdynamic natureof mitochondria and regulationof mitochondrial
fusion by Mitofusin.
● Adopted stepwise process to accomplish objectives, resulting in publication featured in Journal of
Biological Chemistry.
PUBLICATIONS
1. Yaron J, Rao M, Gangaraju S, Zhang L, Kong X, Su F, Tian Y, Glenn H, Meldrum D.
The oxindole Syk inhibitor OXSI-2 blocks nigericin-induced inflammasome signaling and pyroptosis
independent of potassium efflux. Biochem Biophys Res Commun. 2016 Apr 8; 472(3):545-50.
doi:10.1016/j.bbrc.2016.03.021. Epub 2016 Mar 9.
2. Yaron J, Gangaraju S, Rao M, Kong X, Zhang L, Su F, Tian Y, Glenn H, Meldrum D. K(+) regulates
Ca(2+) to drive inflammasome signaling: dynamic visualization of ion flux in live cells.
Cell Death Dis. 2015 Oct 29; 6:e1954. doi: 10.1038/cddis.2015.277.
3. GangarajuS, SultanK, WhiteheadSN, Nilchi L, Slinn J, Li X, Hou ST. Cerebral endothelialexpression
of Robo1 affects brain infiltration of polymorphonuclear neutrophils during mouse stroke recovery.
Neurobiol Dis. 2013 Mar 5.
6. Sandhya Gangaraju
Phoenix, AZ • 480 696 0417 • gsraju25@gmail.com
Sandhya Gangaraju Page 6
4. Jagdeep K. Sandhu, Patricia Lanthier, Melissa Beyers, Harvey Miller, Caroline Sodja, Bogdan
Zurakowski, Sandhya Gangaraju, Marianna Sikorska. Therapeutic effects of Ubisol-QE in the 1-
methyl-4-phenyl-1, 2, 3, tetrahydropyridine (MPTP) mouse model of Parkinson’s disease. Submitted
Neurobiology of Aging. 2013.
5. Whitehead SN, Gangaraju S, Aylsworth A, Hou ST. Membrane raft disruption results in neuritic
retraction prior to neuronal death in cortical neurons. Biosci Trends, 6(4):183-91. 2012 Aug.
6. Whitehead SN, Chan KH, Gangaraju S, Slinn J, Li J, Hou ST. Imaging Mass Spectrometry Detection
of Gangliosides species in the Mouse Brain following Transient Focal Cerebral Ischemia and Long-
Term Recovery. PLoS One 6(6):e20808. Epub. 2011 Jun 8.
7. Shawn N Whitehead, Sandhya Gangaraju, Jacqueline Slinn, Sheng T Hou. Transient and bilateral
increase in Neuropilin-1, Fer kinase and collapsin response mediator proteins within membrane rafts
following unilateral occlusion of the middle cerebral artery in mouse. Brain Research, 1344:209-16.
2010.
8. Jagdeep K. Sandhu; Mossa Gardaneh, PhD; Rafal Iwasiow, PhD; Patricia Lanthier, BSc; Sandhya
Gangaraju, MSc; Maria Ribecco-Lutkiewicz, PhD; Roger Tremblay, BSc; Kazutoshi Kiuchi, PhD;
Marianna Sikorska, PhD. Astrocyte-secreted GDNF and Glutathione antioxidant system protects
neurons against 6OHDAcytotoxicity. Neurobiology of Disease, vol 32. 2008.
9. Mahmud Bani-Yaghoub, Roger G. Tremblay, Abdellah Ajji, Munyao Nzau, Sandhya Gangaraju,
David Chitty, Bogdan Zurakowski, Marianna Sikorska. Neuroregenerative strategies in the brain:
emerging significance of bone morphogenetic protein 7 (BMP7). Biochem and Cell Biol, vol. 86,
number 5, pages 361-369. October 2008.
10. Margaret Neuspiel, Rodolfo Zunino, Sandhya Gangaraju, Peter Rippstein, Heidi McBride. Activated
Mitofusin 2 Signals Mitochondrial Fusion, Interferes with Bax Activation, and Reduces Susceptibility
to Radical Induced Depolarization. J Biol Chem, vol. 280, issue 16, 25060-25070.2005 July 1.
COMPUTER SKILLS
● Adobe Photoshop
● CorelDraw
● GraphPad prism
● NIS-Elements Confocal
● StepOne™
● Microsoft Excel, Word & PowerPoint