Seminarios biología molecular- Manuela Rios Ramirez

1. Manuela Ríos Ramírez
III Semestre- UPB
Biología Molecular

2. Table of Contents
Methods Results
Introduction General Objetive
04
02
03
01
05 Discussion 06 Conclusion

3. Introduction
01

4. Introduction
Osteoblast
Mitochondria
● Cells in charge of
synthesizing and secreting
the organic part of the bone
matrix, during bone
formation and regeneration.
● Essential organelles for
eukaryotic cells that are in
charge of energy production,
storage of calcium ions, and
regulation of cell death

5. Introduction
PINK 1
● PTEN-induced kinase 1 is a
mitochondrial protein of the
serine/threonine kinase type-
Osteoporosis
● Weakening of the bones,
making them brittle.

6. General
Objective
02

7. General Objective
“In the current study, we investigated bone mass and
mitochondria in osteogenic cells of ovariectomized mice
with genetic deletion of PINK1.”

8. Methods
03

9. Methods
PCR
● Reacción enzimática in vitro que
amplifica millones de veces una
secuencia específica de ADN durante
varios ciclos repetidos en los que la
secuencia blanco es copiada fielmente.
● Técnica analítica usada para
identificar proteínas específicas en
una mezcla compleja de proteínas, tal
como la que se presenta en extractos
celulares o de tejidos.
Western Blot

10. Methods
RT- PCR
● Su utilidad será para detectar y
cuantificar las secuencias específicas
de ácidos nucleicos mediante el uso de
reporteros fluorescentes en la
reacción.
● Es una técnica de inmunomarcación
que hace uso de anticuerpos unidos
químicamente a una sustancia
fluorescente para demostrar la
presencia de una determinada
molécula.
Fluorescencia

11. Results
04

12. Results
FIGURA 2 FIGURA 3

13. Results
FIGURA 6

14. Discussion
05

15. Discussion
Author Proposal Opinion
Almeida M, Han L, Ambrogini E, Bartell SM,
Manolagas SC.
Estrogens and androgens are known to
serve as protectors of osteoblasts by
reducing oxidative stress in the
extracellular-signal-regulated kinase
(ERK)-dependen
Ge P, Dawson VL, Dawson TM. In addition, PINK1 stimulates the
generation of new mitochondria as
replacement
Gehrke S, Bertolin G Furthermore, the roles of PINK1 in the
translation of mitochondrial RNAs and
import of the proteins have also been
proposed in yeast and humans, for driving
the local supply quickly

16. Conclusion
06

17. Conclussions
1. From the study: this study demonstrate that the suppression in the activity of PINK1 during osteoblast
differentiation , it's related to the mitochondrial quality control and the production of ROS.
2. Personal:
● Continuing with the investigation of how PINK1 is crucial in the differentiation of
osteoblasts, could be a therapeutic and preventive focus based on its molecular behavior, in
the future of the treatment of a disease as prevalent as osteoporosis.
● Highlighting the importance of molecular biology techniques in understanding the behavior
of the human body at the most detailed level, gives us as doctors the possibility of
implementing new strategies aimed at treating diseases from their molecular origin. This
allows future expansion of the fields of knowledge of the human body at the research level
and in disease prevention, generating an enormous impact on world health.

18. MAPA CONCEPTUAL