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Wheat
Rust Management
Sana Salih
10 April 2014
Outlines
Rust and Yield
Types of Rust
Methods of Control
Conclusion
• Breeding for Rust Resistance
• Genetic Modification
• Biological Control of Rust
• Agricultural Practices
• Fungicides
Effect of Rust on Yield
 Reduce Photosynthesis
 Use Nutrients
 Increase Respiration
 Growth and Seed Fill
Yield Loss
- LR 50 %
- YR 70 %
- SR 100 %
(Carretero et al., 2011; Kolmer et al., 2009)
Types of Rust
Leaf Rust
Puccinia triticina
Symptoms
• brown to orange color pustules
• leaf sheath, peduncles
• ring or a chlorotic halo around the pustules
• 10 – 30 0C
(Kolmer, 2013)
Types of Rust
www.cropwatch.unl.edu
Symptoms
 normally on mature leaves
 yellow-orange pustules
 between the vines
 later on the stripes turn on black
 2-15 0C
Stripe (Yellow) Rust
Puccinia striiformis f.sp. tritici
Types of Rust
www.cropwatch.unl.edu
Stem Rust
Puccinia graminis f.sp. tritici
Symptoms
 lesions in yellow-orange colour
 leaves, leaf sheath and ears
 15-35 0C
Methods of Control
1. Breeding for Rust Resistance
Common Wheat
(Marquis)
Goatgrass
(Aegilops speltoides Tausch)
(Sr39, Lr35)
(Niu et al.2011; Xu et al., 2009)
RL5711 x Thatcher
X
Chinese Spring x RL6082
ph1b mutant
Methods of Control
2. Genetic Modification
• vst1 and vst2 from grapevine
• Particle bombardment Immature embryo
(Florida , Combi)
• LR 42-72%
(Serazetdinova et al., 2005)
Methods of Control
3. Biological Control of Rust
• Bacteria - Bacillus subtilis E1R-j (CYR32)
• antimicrobial (iturins, fengycins, surfactins)
• Chinese winter wheat, Mingxian 169
• spore : below 46% in treated 76% in non-treated
(Li et al., 2013)
Methods of Control
4. Agricultural Practices
• Cultivar Mixtures
Syria 20-70 % Turkey <20% in 2010 (2011 ICARDA)
• Alternate Hosts
(Huang et al., 2011; Jin et al., 2010)
Methods of Control
5. Fungicides
1- Seed Dressing - fluquinconazole, prochloraz
2- Spraying - pyraclostrobin, epoxiconazole + isopyrazam, metconazole
• Match the disease
• Fungicides Mixture – Different Mode of Action
(HGCA, 2013)
Conclusion
Using MAS breeding program
New Source Of Resistance Gene (Old Varieties, Relative Species)
More Than One Resistance Gene In New Cultivar
Diversify Cropping
References
Carretero, R., Bancal, M. and Miralles, D. (2011)‘Effect of leaf rust (Puccinia triticina) on photosynthesis and related
processes of leaves in wheat crops grown at two contrasting sites and with different nitrogen levels’. European Journal of
Agronomy 35, 237– 246
Feng, P., Baley, G., Clinton, W., Bunkers, G., Alibhai, M., Paulitz, T. and Kidwell, K. (2005) ‘Glyphosate inhibits rust diseases in
glyphosate-resistant wheat and soybean’ Proceedings of the National Academy of Sciences 102, 17290 –17295
German, S. and Kolmer, J. (2014) ‘Leaf rust resistance in selected late maturity, common wheat cultivars from Uruguay’.
Euphytica 195, 57–67
German, S., Barcellos, A., Chaves, M., Kohli, M., Campos, P. and Viedma, L. (2007) ‘The situation of common wheat rusts in the
Southern Cone of America and perspectives for control’. Australian Journal of Agricultural Research 58, 620–630
Gustafson, P., Raskina, O., Ma, X. and Nevo, E. (2009) ‘Wheat Evolution, Domestication and Improvement’. In wheat Science and
Trade. Ed. by Carver, B. Wiley Blackwell, 5-30
Hsieh, F., Lin, T., Meng, M. and Kao, S. (2008) ‘Comparing Methods for Identifying Bacillus Strains Capable of Producing the
Antifungal Lipopeptide Iturin A’. Current microbiology 56, 1–5
Huang, C., Sun, Z., Wang, H., Luo, Y. and Ma, Z.(2012) ‘Spatiotemporal effects of cultivar mixtures on wheat stripe rust
epidemics’. The European Journal of Plant Pathology 131, 483–496
Huang, C., Sun, Z., Wang, H., Luo, Y. and Ma, Z.(2009) ‘Effects of wheat cultivar mixtures on stripe rust: A meta-analysis on field
trials’. Crop Protection 33, 52-58
Jin, Y., Szabo, L. and Carson, M. (2010) ‘Century-old mystery of Puccinia striiformis life history solved with the identification of
Berberis spp. as an alternate host´. Phytopathology 100, 432-435
Khan, M., Bukhari, A., Dar, Z. and Rizvi, S. (2013) ‘Status and strategies in breeding for rust resistance in wheat’. Agricultural
Sciences 4, 292-301
Kolmer, J., Chen, X. and Jin, Y. (2009) ‘Diseases which challenge global wheat production-the wheat rust’. In wheat Science and
Trade. Ed. by Carver, B. Wiley Blackwell, 89-100
Kolmer, J., Ordonez, M. and Groth, J. (2009) ‘The Rust Fungi’ In: Encyclopedia of Life Sciences (ELS). John Wiley & Sons, Ltd:
Chichester
Li, H., Zhao, J., Feng, H., Huang, L. and Kang, Z. (2013) ‘Biological control of wheat stripe rust by an endophyticBacillus
subtilisstrain E1R-j in greenhouse and field trials’. Crop Protection 43, 201-206
Marasas, C., Smale, M. and Singh, R. (2003) ‘The economic impact of productivity maintenance research: breeding for leaf rust
resistance in modern wheat’. Agricultural Economics 29, 253–263
Nayar, K., Bhardwaj, C. and Jain, K. (2002) ‘Fungal Diseases of Wheat and Barley-Rusts ’. in Diseases of Field Crops. ed. by
Gupata, V.K. and Paul, Y.S. Indus publishing, 17-25
Niu, Z., Klindworth, D., Friesen, T., Chao, S., Jin, Y., Cai, X. and Xu, S. (2011) ‘Targeted Introgression of a Wheat Stem Rust
Resistance Gene by DNA Marker-Assisted Chromosome Engineering’. Genetics Society of America 187, 1011–1021
Serazetdinova, L., Oldach, K. and Lorz, H. (2005) ‘Expression of transgenic stilbene synthases in wheat causes the accumulation
of unknown stilbene derivatives with antifungal activity’. Journal of Plant Physiology 162, 985—1002
Singh, R., Foessel, S., Espino, J., Singh, S., Bhavani, S., Lan, C. and Basnet, B. (2014) ‘Progress Towards Genetics and Breeding for
Minor Genes Based Resistance to Ug99 and Other Rusts in CIMMYT High-Yielding Spring Wheat’. Journal of Integrative
Agriculture 13(2), 255-261
Xue, G., Chi, D., Shu-zhen, Z. and Zuo-fu, L. (2012) ‘Wheat Production and Wheat Rust Management in Canada’. Journal of
Northeast Agricultural University (English edition)19 (1), 1–14
Xu, S., Jin, Y., Klindworth, D., Wang, R. and Cai, X. (2009) ‘Evaluation and Characterization of Seedling Resistances to Stem Rust
Ug99 Races in Wheat–Alien Species Derivatives’. Crop Science 49, 2167–2175

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Wheat Rust Management

  • 2. Outlines Rust and Yield Types of Rust Methods of Control Conclusion • Breeding for Rust Resistance • Genetic Modification • Biological Control of Rust • Agricultural Practices • Fungicides
  • 3. Effect of Rust on Yield  Reduce Photosynthesis  Use Nutrients  Increase Respiration  Growth and Seed Fill Yield Loss - LR 50 % - YR 70 % - SR 100 % (Carretero et al., 2011; Kolmer et al., 2009)
  • 4. Types of Rust Leaf Rust Puccinia triticina Symptoms • brown to orange color pustules • leaf sheath, peduncles • ring or a chlorotic halo around the pustules • 10 – 30 0C (Kolmer, 2013)
  • 5. Types of Rust www.cropwatch.unl.edu Symptoms  normally on mature leaves  yellow-orange pustules  between the vines  later on the stripes turn on black  2-15 0C Stripe (Yellow) Rust Puccinia striiformis f.sp. tritici
  • 6. Types of Rust www.cropwatch.unl.edu Stem Rust Puccinia graminis f.sp. tritici Symptoms  lesions in yellow-orange colour  leaves, leaf sheath and ears  15-35 0C
  • 7. Methods of Control 1. Breeding for Rust Resistance Common Wheat (Marquis) Goatgrass (Aegilops speltoides Tausch) (Sr39, Lr35) (Niu et al.2011; Xu et al., 2009) RL5711 x Thatcher X Chinese Spring x RL6082 ph1b mutant
  • 8. Methods of Control 2. Genetic Modification • vst1 and vst2 from grapevine • Particle bombardment Immature embryo (Florida , Combi) • LR 42-72% (Serazetdinova et al., 2005)
  • 9. Methods of Control 3. Biological Control of Rust • Bacteria - Bacillus subtilis E1R-j (CYR32) • antimicrobial (iturins, fengycins, surfactins) • Chinese winter wheat, Mingxian 169 • spore : below 46% in treated 76% in non-treated (Li et al., 2013)
  • 10. Methods of Control 4. Agricultural Practices • Cultivar Mixtures Syria 20-70 % Turkey <20% in 2010 (2011 ICARDA) • Alternate Hosts (Huang et al., 2011; Jin et al., 2010)
  • 11. Methods of Control 5. Fungicides 1- Seed Dressing - fluquinconazole, prochloraz 2- Spraying - pyraclostrobin, epoxiconazole + isopyrazam, metconazole • Match the disease • Fungicides Mixture – Different Mode of Action (HGCA, 2013)
  • 12. Conclusion Using MAS breeding program New Source Of Resistance Gene (Old Varieties, Relative Species) More Than One Resistance Gene In New Cultivar Diversify Cropping
  • 13.
  • 14. References Carretero, R., Bancal, M. and Miralles, D. (2011)‘Effect of leaf rust (Puccinia triticina) on photosynthesis and related processes of leaves in wheat crops grown at two contrasting sites and with different nitrogen levels’. European Journal of Agronomy 35, 237– 246 Feng, P., Baley, G., Clinton, W., Bunkers, G., Alibhai, M., Paulitz, T. and Kidwell, K. (2005) ‘Glyphosate inhibits rust diseases in glyphosate-resistant wheat and soybean’ Proceedings of the National Academy of Sciences 102, 17290 –17295 German, S. and Kolmer, J. (2014) ‘Leaf rust resistance in selected late maturity, common wheat cultivars from Uruguay’. Euphytica 195, 57–67 German, S., Barcellos, A., Chaves, M., Kohli, M., Campos, P. and Viedma, L. (2007) ‘The situation of common wheat rusts in the Southern Cone of America and perspectives for control’. Australian Journal of Agricultural Research 58, 620–630 Gustafson, P., Raskina, O., Ma, X. and Nevo, E. (2009) ‘Wheat Evolution, Domestication and Improvement’. In wheat Science and Trade. Ed. by Carver, B. Wiley Blackwell, 5-30 Hsieh, F., Lin, T., Meng, M. and Kao, S. (2008) ‘Comparing Methods for Identifying Bacillus Strains Capable of Producing the Antifungal Lipopeptide Iturin A’. Current microbiology 56, 1–5 Huang, C., Sun, Z., Wang, H., Luo, Y. and Ma, Z.(2012) ‘Spatiotemporal effects of cultivar mixtures on wheat stripe rust epidemics’. The European Journal of Plant Pathology 131, 483–496 Huang, C., Sun, Z., Wang, H., Luo, Y. and Ma, Z.(2009) ‘Effects of wheat cultivar mixtures on stripe rust: A meta-analysis on field trials’. Crop Protection 33, 52-58 Jin, Y., Szabo, L. and Carson, M. (2010) ‘Century-old mystery of Puccinia striiformis life history solved with the identification of Berberis spp. as an alternate host´. Phytopathology 100, 432-435 Khan, M., Bukhari, A., Dar, Z. and Rizvi, S. (2013) ‘Status and strategies in breeding for rust resistance in wheat’. Agricultural Sciences 4, 292-301
  • 15. Kolmer, J., Chen, X. and Jin, Y. (2009) ‘Diseases which challenge global wheat production-the wheat rust’. In wheat Science and Trade. Ed. by Carver, B. Wiley Blackwell, 89-100 Kolmer, J., Ordonez, M. and Groth, J. (2009) ‘The Rust Fungi’ In: Encyclopedia of Life Sciences (ELS). John Wiley & Sons, Ltd: Chichester Li, H., Zhao, J., Feng, H., Huang, L. and Kang, Z. (2013) ‘Biological control of wheat stripe rust by an endophyticBacillus subtilisstrain E1R-j in greenhouse and field trials’. Crop Protection 43, 201-206 Marasas, C., Smale, M. and Singh, R. (2003) ‘The economic impact of productivity maintenance research: breeding for leaf rust resistance in modern wheat’. Agricultural Economics 29, 253–263 Nayar, K., Bhardwaj, C. and Jain, K. (2002) ‘Fungal Diseases of Wheat and Barley-Rusts ’. in Diseases of Field Crops. ed. by Gupata, V.K. and Paul, Y.S. Indus publishing, 17-25 Niu, Z., Klindworth, D., Friesen, T., Chao, S., Jin, Y., Cai, X. and Xu, S. (2011) ‘Targeted Introgression of a Wheat Stem Rust Resistance Gene by DNA Marker-Assisted Chromosome Engineering’. Genetics Society of America 187, 1011–1021 Serazetdinova, L., Oldach, K. and Lorz, H. (2005) ‘Expression of transgenic stilbene synthases in wheat causes the accumulation of unknown stilbene derivatives with antifungal activity’. Journal of Plant Physiology 162, 985—1002 Singh, R., Foessel, S., Espino, J., Singh, S., Bhavani, S., Lan, C. and Basnet, B. (2014) ‘Progress Towards Genetics and Breeding for Minor Genes Based Resistance to Ug99 and Other Rusts in CIMMYT High-Yielding Spring Wheat’. Journal of Integrative Agriculture 13(2), 255-261 Xue, G., Chi, D., Shu-zhen, Z. and Zuo-fu, L. (2012) ‘Wheat Production and Wheat Rust Management in Canada’. Journal of Northeast Agricultural University (English edition)19 (1), 1–14 Xu, S., Jin, Y., Klindworth, D., Wang, R. and Cai, X. (2009) ‘Evaluation and Characterization of Seedling Resistances to Stem Rust Ug99 Races in Wheat–Alien Species Derivatives’. Crop Science 49, 2167–2175