1. ACADEMIC REPORTING FORM
September 2011 – September 2012
1. Name of Participation : Romi Novriadi
2. N I P : 19811111 200502 1 002
3. Name of Institution : Batam Mariculture Development Centre
Balai Budidaya Laut Batam
Directorate General of aquaculture
Ministry of Fisheries and Marine Affairs
Republic of Indonesia
4. Program of Study : Master degree
5. Degree objectives : Mater of Science in Aquaculture
6. Name of University : Universiteit Gent
7. Country : Belgium
8. Number of Credit Hours : 60 ECTS (European Credit Transfer System)
9. Requirements completed : 120 ECTS (European Credit Transfer System )
10. Academic advisors name :
1. Prof. Dr. Patrick Sorgeloos
Professor, Program Director
Address : Faculty of Bioscience Engineering
Department of Animal Production
Laboratory of Aquaculture and Artemia Reference Center (ARC) - Ghent University
Rozier 44, B-9000 Gent, Belgium
Tel : +32-9-2643754; Fax : +32-9-2644193
E-mail : patrick.sorgeloos@ugent.be
2. Prof. Dr. ir. Peter Bossier
Professor, Project Manager
Address : Faculty of Bioscience Engineering
Department of Animal Production
Laboratory of Aquaculture and Artemia Reference Center (ARC) - Ghent University
Rozier 44, B-9000 Gent, Belgium
Tel : +32-9-2643759; Fax: +32-9-2644193
E-mail : peter.bossier@ugent.be
11. Course completed in previous year
First Semester
Course Lecturer A B C D E
Aquatic Ecology Prof. Colin Janssen 30.0 30.0 0 135 5
Biology of Aquatic Organisms Prof. Dominique Adriaens 30.0 15.0 0 120 4
Freshwater Fish Culture
Dr. Nancy Nevejan 45.0 30.0 0 180 6
Techniques
General Aspects of
Prof. Patrick Sorgeloos 15.0 30.0 0 120 3
Aquaculture
Microbial Ecology and
Prof. Korneel Rabaey 30.0 15.0 0 120 4
Environmental Sanitation
Technology of Fishery
Prof. Frank Devlieghere 15.0 15.0 0 75 3
Products
Applied Statistics Prof. Olivier Thas 15.0 45.0 0 125 5
875 30

2. Second semester
Course Lecturer A B C D E
Physiology of Aquatic
Prof. Gudrun De Boeck 15.0 15.0 0 75 3
Organisms
Algae Culture Prof. Peter Bossier 15.0 15.0 0 75 3
Aquatic Farm Management
Prof. Peter Bossier 15.0 45.0 0 125 5
Training
Aquaculture Nutrition Dr. Gilbert Van Stappen 3
Mollusc and Crustacean
Dr. Nancy Nevejan 45.0 45.0 0 202 5
Culture
Aquaculture and the
Prof. Peter Bossier 45.0 15.0 0 150 5
Environment
Marine Fish Larviculture Dr. Gilbert Van Stappen 45.0 30.0 0 180 6
807 30
12. Course to be taken in upcoming semester
Course Lecturer A B C D E
Management in the
Prof. Peter Bossier 45.0 15.0 0 150 5
Aquaculture Industry
Aquaculture Genetics Prof. Peter Bossier 45.0 15.0 0 150 5
Diseases in Aquaculture Prof. Peter Bossier 45.0 15.0 0 150 5
Master Dissertation NN 0 0 300 900 30
13. Acedemic advisor’s comments on Participants Progress
..............................................................................................................................................
..............................................................................................................................................
.............................................................................................................................................
.............................................................................................................................................
14. Internship Work
Tittle : Shrimp Post Larvae Sampling Campaign in Commercial Hatcheries
Date : 9 of July – 20 of August 2012
Location : INVE Shrimp Culture research and Development
Chonburi – Thailand
Report : Attached
Brief summary :
Post larva sampling campaign in the commercial hatcheries gives a significant information
about the best management practices in shrimp hatchery to produce the best quality of
post larvae. Generally shrimp hatcheries in Thailand are a medium-scale backyard hatchery
and honesty is the main key to success in this business. Positive respond shown by the
shrimp farmer when we visit their hatchery and we discuss about the tricks that can be
applied to solve the problem, especially the zoea syndrome in shrimp larvae production.
The main obstacle in the discussion is the language, but this problem can be solved
properly by the INVE SCRD staff. During internship, I also learned about the culture in
Thailand, and how the communities in Thailand build a harmony among themselves.

3. Together with SCRD staff we also visit Kasetsart University Sriracha fisheries research
station and discuss about the current technology in Aquaculture development.
15. Thesis Work
Tittle : Immune parameters (Toolbox for Artemia)
The innate system of invertebrates recognizes the conserved pathogen-associated
molecular patterns (PAMP’s), as for example lipopolysaccharides from bacteria and β-1,3-
glucans from fungi, through a series of pattern recognition receptors (PRRs),
(Jiravanichpaisal et al., 2006). The innate immune system can be divided into humoral and
cellular defense responses; humoral defenses consists out of production of antimicrobial
peptides (AMPs), reactive intermediates of oxygen or nitrogen and complex enzymatic
cascades that regulate clotting or melanization of the hemolymph (Beutler 2004). Cellular
defense mechanisms are constituted out of several steps: first there is chemotaxis, or
active migration of hemocytes; followed by opsonization, or secretion of soluble factors
(AMPs) which act directly on the microbial membrane against a broad range of
microorganisms (Bachère 2003; Beutler 2004; Donaghy et al., 2009), followed by
phagocytosis (the internalization and degradation of foreign material) and ‘oxidative or
respiratory burst’; during respiratory burst a rapid release of oxygen reactives occurs
(superoxide radical and hydrogen peroxide) and immune cells use NADPH oxidase to
reduce O2 to oxygen free radicals and then H2O2. When overstimulation of the immune
system occurs, there is a reduction in circulating hemocyte numbers leaving the host’s
ability to deal with infection diminished (Bachère et al., 1995).
Phenoloxidase (PO) is an oxidoreductase able to mount an early rapid response to a
pathogen. The immune system is following up with slower processes such as cellular
defense and synthesis of AMPs (Söderhäll & Cerenius, 1998). The prophenoloxidase
activating cascade (proPO-system) also called the melanization cascade is situated in the
hemocytes and is an important immune mechanism in crustaceans (Bachère et al., 1995;
Cerenius et al., 2008). The conversion of prophenoloxidase to phenoloxidase is triggered by
external cues such as immunostimulants leading to the assembly of cytotoxic products (i.e.
quinine substances), melanin production and encapsulation of pathogens (Söderhall and
Cerenius, 1998). Different quinone intermediates have different toxicity (Cerenius et al.,
2008) and excessive production of quinone compounds is likely to result in toxicity and
deleterious effects on the host tissue; therefore there is an elaborate system of activating
factors and proteinase inhibitors to control proPO activation spatially and temporally
(Cerenius et al., 2008).
The immune system of crustaceans has been intensively studied and there is no reason to
suspect that the basic functioning of the immune system in Artemia should be different.
However at the level of gene expression not much is known yet for Artemia. There are
40.000 sequences available at public databases (i.e. the website of the European
Bioinformatics Institute), however these sequences are frequently too short to annotate
the gene function (they are generated on the basis of EST libraries) and hence are less
successful in the development of a RT-PCR platform. Thanks to our collaboration with the
Beijing Genomics Institute, a major sequencing effort will be launched for Artemia
resulting in a larger coverage of the genome and hopefully even the complete sequenced
genome of Artemia will be to our disposal for further research on homologue gene
sequences. Based on the information available on genes in the innate immune system in
other vertebrates and invertebrates, Artemia homologues can be identified and real time-
PCR protocols can be developed. Although these protocols are necessary for testing on the
mRNA level and to develop a platform together with producing new markers, verification
of immune activity at the protein activation level is also needed. This can be done using a
toolbox that is already commercially available. Enzyme activity can be tested via a DOPA
test for phenoloxidase (PO) activity, nitric oxide synthase (NOS) or superoxide dismutase
(SOD) activity can be detected using commercially existing kits.
Gent, 12 September 2012
Signature of Academic Advisor

4. (....................................................)