Hands on Training, 2016, college of fisheries kawardha,chhattisgarh

1. HANDS ON TRAINING
ON
SEED PRODUCTION IN
FISHERIES
PREPAERD BY:
Ritesh Chandravanshi
Rohit kumar Painkra
Shubham Sahu
Sunil Kumar Sahu

2. FACULTY MEMBERS
CEO of programme - Dr. H.K.Vardia (Dean COF, Kawardha)
Programme manager - Dr. Honnananda B.R. (Asst. Professor)
Advisory Members - Dr. Niranjan Sarang (Asst. Prof.)
Sh. Pabitra Barik (Asst. Prof.)
Sh. Dushyant Kumar Damle (Asst. Prof.)
Dr. B.Nightingale Devi (Asst. Prof.)
Sh. Kamalesh Panda (Asst. Prof.)
Mr. Anirudh Kumar (Course Teacher)

3. S. No. Objective Site of Work
1.
Carp Breeding and Seed
Production
Carp Seed Production and
Rearing Center, Bodla and
Carp Seed Rearing Center,
Khairbanakala, Kawardha
2. Nursery Rearing of Carp Seeds
Fish Seed Rearing Center,
Khairbanakala, Kawardha
3. Magur Breeding
Magur Breeding and Seed
Production Center, Gaurmati
4.
Ornamental Fish Breeding and
Culture
Live Fish Laboratory, College
of Fisheries, Kawardha
LEARNING OBJECTIVE UNDER HANDS ON TRAINING

4. CARP BREEDING AND SEED PRODUCTION

5. ✓ Carps are the main stay of aquaculture in India & as a matter of fact,
India is called as the “Carp Country”.
✓ Previously fishes are bred naturally, then pituitary gland extract was
used but now a days synthetic hormone ( i.e. Ovaprim, Ovatide
(Haemopharma), Ovapel and WOVA-FH ) was used for breeding.
✓ Brazil was the first country to develop hypophysation technique in
1934.
✓ In India, Prof. Hiralal Chaudhary suceeded in induced breeding in
1955.
INTRODUCTION

6. SITE OF WORK
1. Carp Seed Production &
Rearing Center, Bodla,
Kawardha
2. Carp Seed Rearing Center,
Khairbana kala, Kawardha.

7. CARP SEED PRODUCTION & REARING CENTER, BODLA
R
O
A
D
C
O
M
P
L
E
X
NURSERY POND REARING POND BROODER POND
Total water area - 12541.4 square meter
Total land area - 7458.6 square meter
POND
9
POND
4
POND
1
POND
2
POND
3
POND
6
POND
7
PON
D 8
POND
5
HATCHER
Y

8. 6.06
m
5.64m 3.2m
1.46m
0.42m
Outlet
7.88m
1.3m
8.8
m
3.51m
2.01m
1.5
m
1.3m
90cm
4.3
m
Over head tank
0.47m
Spawning tank
INCUBATION UNIT
1.56m
2.2m
1.9 feet
3.3 inch
SPAWN COLLECTION TANK

9. S.no Component Outer
diamete
r
(m)
Inner
diameter
(m)
Height
(m)
Wall
thickness
(m)
Slope
(m)
No. of
inlet
(Duck
mouth)
Distanc
e
betwee
n inlets
(m)
Height
of
outlet
(m)
1. Spawning
pool 6.72 5.95 1.2 0.39 0.23 18 1 0.86
2. Incubation
pool
4.2
3.6
2.27
1.78
1 .04
0.95
0.3
0.06
0.04
14 0.77 0.15
Spawning pool Incubation pool

10. RECTANGULAR UNIT
Components Length (m) Width (m) Height (m) Area(m2) Volume (m3)
Egg collection
tank
2.5 1.4 0.9 3.5 3150
Spawn collection
tank
2.0 1.19 0.86 2.38 2040
Over head tank 8.43 5.10 1.45 42.99 62330
Spawn Collection Tank Over Head Tank

11. MORPHOMETRY OF POND
POND 1 POND 2 POND 3 POND 4 POND 5 POND 6 POND 7 POND 8
POND
9
Length
(m)
25.6 33 39.6 43 108 54 53 36 120
Width
(m)
20.5 12.7 12.6 15.6 40 52 52 15 120
Slope 1:2 1:2 1:1 1:1 1:3 1:3 1:3 1:1 1:2
Free
board
(m)
1.0 1.0 1.5 1.5 2.0 3.0 3.0 1.3 3.0
Depth
(m)
1.7 1.5 2.0 2.0 3.5 4.0 4.0 2.0 5.0
Water
level
(m)
0.7 0.55 0.5 0.5 1.5 1.0 1.0 0.7 2.0
Area
(m2 )
525 419 499 671 4320 2808 2756 540 14400
Volume
(m3)
367 231 249 335 6480 2808 2765 378 28800

12. PICTORIAL VIEW OF MEASUREMENT OF HATCHERY COMPONENT & POND
Measurement of
Spawning Pool
Measurement of Pond
Depth
Measurement of Free Board of
Pond
Measurement of Pond Length
Measurement of
Incubation Tank

13. BREEDING SET
INDIAN MAJOR CARP SEED PRODUCTION,
FISH SEED PRODUCTION & REARING CENTER,
Bodla, Kawardha
S.no. Date
Weight of
Brooder(kg)
Total no. of
Eggs(lakh)
No. of Fertilized
eggs
No. of opaque
Eggs
Fertilization
rate
Male Female
1 05/08/16 33.5 27.5 36.7 515 41 92.62%
2 07/08/16 41.5 52 76.6 525 52 90.98%
INDIAN MAJOR CARP SEED PRODUCTION,
FISH SEED PRODUCTION & REARING CENTER,
khairbana, Kawardha
S.no. Date
Weight of
Brooder(kg)
Total no. of
Eggs(lakh)
No. of Fertilized
eggs
No. of
opaque Eggs
Fertilization
rate
Male Female
1 26/07/16 22 40 60 545 21 96.28%

14. INDUCED BREEDING & SEED PRODUCTION OF CARPS
Steps Followed are
1. Collection of brooders.
2. Transfer of brooders to ante tank.
3. Segregation of male & female broods in ante
tank.
4. Preparation of Hormonal Injection.
5. Administration of Hormone.
6. Transfer of Injected Brooders into Breeding Pool
7. Stripping
8. Transfer of eggs in incubation pool.
9. Collection of spawn.
10. Packaging of spawn & Transportation.

15. PICTORIAL REPRESENTATION OF STEPS FOLLOWED FOR INDUCED
BREEDING AND SEED PRODUCTION OF CARPS
1. Collection of
Brooders
2. Transfer of Brooders to Ante
Tank
3. Segregation of Male &
Female

16. 4. Preparation of Hormonal Injection
5. Administration of Hormone6.Transfer of Injected Brooders into
Breeding Pool
Dose of synthetic hormone used is 0.2 & 0.3 mg/kg body wt. Of male & female
respectively.

17. c. Proper mixing of ova &
milt
b. Wet stripping
Fertilized eggs
a. Brood fish
7. Stripping
CONT…..

18. Estimation of Fertilization Fercentage:-
8.Transfer of eggs in incubation pool
Fertilized egg Unfertilized egg
Average fertilization
percentage is 93.22 %.

19. S.No. PICTURE TIME CHARACTER S.No. PICTURE TIME CHARACTER
1.
Few second
Fertilization
Swollen
fertilized egg
5.
5:52 hr
after
fertilization
Gastrula stage
2.
50-55 min
after
fertilization
cleavage 6.
11:30 hr
after
fertilization
‘C’ Shaped
embryo
3.
2:40 hr
after
fertilization
morula stage 7.
14:00 hr
after
fertilization
Egg ready for
hatching
(twitching
movement)
4.
4:38 hr
after
fertilization
blastula stage 8.
16:00 hr
after
fertilization
Hatching
EMBRYONIC DEVELOPMENTAL STAGES

20. 9.Collection of Spawn

21. 10. Packaging of Spawn & Transportation
2/3 oxygen
1/3 water
Preparation of Seed
Packet Water Filled
bag
Oxygen Filling & tyingTransportation

22. NURSERY REARING OF CARPS

23. INTRODUCTION
❖The nursery rearing of carp seed was done for culture of spawn up to fry
stage.
❖Rearing of 3 to 4 days old spawn of size 5-6 mm of carp seed was done in
nursery ponds for 15 to 20 days.
❖For rearing of spawn earthen ponds was used and the allotted pond
measurement is listed below in table:-
Pond measurements
Sl. No. Length
(m)
Width
(m)
Water
level
(m)
Slope
(m)
Free board
(m)
Area
(m2)
2. 62.0 26.1 0.9 1:2 0.9 1618.2
3. 62.0 26.1 0.9 1:2 0.9 1618.2
4. 62.0 26.1 1.0 1:2 1.0 1618.2

24. FISH SEED REARING
CENTER
KHAIRBANAKALA, KAWARDHA
SITE OF WORK

25. PN7
PN8
PN9
PN10
PN11PN12PN13PN14
PN15
PN16
PN5
PN19
PN20
SARODHARESORVIORROAD
PN18
PN1
PN6
PN2
HATCHERY COMPLEX
Store Room
PN17
PN21
LAYOUT OF FISH SEED REARING CENTER, KHAIRBANAKALA
Brooder
pond
Rearing
pond
Nursery pond
PN= Pond
PN3 PN4
PRIMARY AREA=27,212.35 m2 SECONDARY AREA=13,449.88 m2

26. CIRCULAR HATCHERY UNIT
Breeding pool Incubation pool
S. No. Properties Measurement S. No. Properties Measurement
1. Diameter (m) OUTER CHAMBER
Inner 7.87 1 Diameter(m) Inner 2.1
Outer 8.26 Outer 4.3
2. Height (m)
1.09
2 Height(m) 1.07
3 No. of duck Mouth 16
4 Volume(m3) 60.38
3. Slope 1:3.9 Inner chamber
4. Wall thickness (m)
0.39
1 Diameter(m) Inner
1.4
5. No. of inlet 16 Outer 3.55
6. Volume (m3) 24.21 2 Volume(m3) 37.59
3 Height(m) 0.95
Unit/ Measurement
Length
(m)
Width
(m)
Height
(m)
Inlet diameter(inch)
Height of
inlet(m)
1. Egg collection tank 2.6 1.82 1 2.5 0.5
1. Spawn collection tank 3.93 2. 41 1.25 3.5 0.75
1. Over head tank 5.5 2.78 1.45 15.29 21.37
RECTANGULAR UNIT

27. MORPHOMETRY OF POND
S.
No.
Ponds 1 2 3 4 5 6 7 8 9 10
1. Length (m) 57 62 62 62 32 36 47.5 34 41 52
2. Width (m) 27 26.1 26.1 26.1 27 31 33 15 40 18
3. Slope (m) 1:2 1:2 1:2 1:2 1:3 1:1.5 1:3.4 1:2.5 1:3 1:2.5
4. Free board (m)
_ 0.9 0.9 1.0 1.2 0.8 2 1.2 1.2 1.5
5. Water level(m) Dry 0.9 0.9 1.0 1 1 1.2 1.5 1.2 0.5
6. Area (m2) 1539 1618.2 1618.2 1618.2 864 1116 1567.5 510 1640 936
7. Volume (m3) _ 2912.76 2912.76 3236.4 1900.8 2008.8 5016 1377 3936 1872
S.
No.
Ponds 11 12 13 14 15 16 17 18 19
20 21
1. Length (m) 29 27 29 37 78 60 35 59.5 22.5 47 35
2. Width (m) 26 24 28 35 34 31 28.5 29 21.5 35.5 24
3. Slope (m) 1:3 1:3 1:2.5 1:2.5 1:3.5 1:3.5 1:2.7 1:1 1:3.5 1:2.5 1:2.5
4.
Free board (m)
2.4 2.5 1.3 1.5 2.5 2 0.9 1.5 1.5 1.1 1.3
5.
Water level (m)
0.6 0.5 0.8 1 1 1 1.6 1.5 1 1 1
6. Area (m2) 754 648 812 1295 2652 1860 997.5 1725.5 483.75 1668.5 840
7.
Volume (m3)
2262 1944 1867.6 3237.5 9282 5580 2493.75 5176.5 1209.3 3503.8 1932

28. CARP SEED REARING
The carp seed rearing can be broadly
divided into:-
1. Pre – Stocking Management
1. Stocking Management
1. Post – Stocking Management

29. PRE- STOCKING MANAGEMENT
Removal of Aquatic Weed
Eradication of Aquatic Insects & Predatory Fish

30. Aquatic Weeds
Ceratophyllum demersum Marselia quadrifolia Lemna minor
Hydrilla verticillata Valisneria sp. Nymphaea alba

31. Aquatic Insects
Nepa sp.
(Water scorpion)
Geries
(Water spider)
Ranatra sp.
(Water stick)
Dragonfly
nymph
Cybister
(Diving beetle)
Belostoma
(Giant bug)

32. Weed Fishes in Pond
Glass fish (Chanda sp.) Gobi sp.
Rasbora sp.Puntius sp.

33. 2. STOCKING MANAGEMENT
We were allotted with three ponds for each group randomly.
Spawn of carp were stocked in nursery pond is 7 Lakh. Where 2 lakh
Spawn stocked in alloted pond no. 2, having an area of about
0.1618 ha.(1618.2 m2 )@123.59 no./m2, 3 lakh spawn stocked in
pond no.3 having an area of about 0.1618ha.(1618 m2) @185.39
no./m2 and 2 lakh Spawn stocked in alloted pond no. 4, having an
area of about 0.1618 ha.(1618.2 m2 ) @123.59 no./m2,

34. POST - STOCKING MANAGEMENT
Supplementary Feeding

35. Water & Soil Quality Parameter Analysis
Sampling of Plankton
Titration Method
Collection of Soil Sample Preperation of Soil Sample for Analysis

36. Parameters/week
1 2 3 4 Mean
Physical
parameters
Temperature (oC) 29 32 28 29 29.5
Chemical
parameters
pH 7.91 8.2 7.5 7.36 7.74
D.O (ppm) 6.28 6.30 5.16 6.13 5.96
Total Alkalinity (ppm) 160.23 166.33 150.0 158.61 158.79
Free C02 (ppm) 2.20 2.13 2.63 2.26 2.30
Total Hardness (ppm) 87.66 88.05 87.09 87.49 87.57
Biology
parameters
Quantitative plankton
estimation (ml/50 L
water)
4.2 4.4 4.0 4.09 4.17
WATER QUALITY PARAMETER ANALYSIS OF IMC POND

37. Tepraturerange
WEEK
Teperature( ̊C)
pHRANGE
WEEK
pH
Dorange
WEEK
Dissolve Oxygen (mg/l)
Alkanityrange
WEEK
Alkalinity(ppm)

38. Freeco2range
WEEK
Free C02 (ppm)
Hardnessrange
WEEK
Hardness(ppm)

39. PARAMETER /WEEK Week 1 Week 2 Week 3 Week 4 MEAN
PHYSICAL PARAMETER
1. SOIL TEXTURE SAND (%) 34.28 42.85 43.10 43.5 41.18
SILT(%) 28.75 30.00 29.50 28.00 28.78
CLAY(%) 28.57 35.71 28.00 29.00 30.32
CHEMICAL PARAMETER
1. ORGANIC CARBON(%) 1.52 1.48 1.43 1.45 1.47
2. pH 8.3 7.6 7.9 7.6 7.8
SOIL QUALITY PARAMETER

40. SAND (%)
SILT(%)
CLAY(%)
Organiccarbon(%)
Week
Organic carbon (%)
pHrange
week
pH

41. LENGTH & WEIGHT MEASUREMENT OF CARP SEED
Measurement of weight
Measurement of length
Sampling of fish

42. LENGTH AND WEIGHT MEASUREMET
Indian Major Carp Seeds
Length and Weight Measurement of Fish Sample
Sl. No. Date Avg. Length (cm) Avg. Weight (gm.)
1. Week 1 1.32 0.143
2. Week 2 2.58 0.29
3. Week 3 3.36 0.44
4. Week 4 3.73 0.75
lengthandweightrange
week
average length
average weight

43. Carp Seeds Packaging and Transportation
Conditioning Packaging
Closed Transportation

44. MAGUR BREEDING AND SEED PRODICTION AT
FISH SEED REARING CENTER-GOURMATI

45. INTRODUCTION
✓ There are three cultivable species of Clarias such as Clarias
batrachus, C.gariepinus, C.macrocephalus.
✓ C. batrachus, commonly known in Asian Mangur and C.gariepinus,
commonly called African Mangur.
✓ The three species are easily distinguishable observing the shape of
occipital process of skull.
✓ They are having accessory respiratory organs by means of which
they can also breath air.

46. LAYOUT OF HATCHERY UNITS
Drainage
Larval Rearing Tank Brood Stock Tank Conditioning
Tank
Platform for flow
through system
Total Water area 7226.2 square meter
Total land area 2777.3 square meter

47. MEASUREMENT OF HATCHERY UNIT
Property Measurement
(m)
Length 1.45
Width 1.42
Height 0.55
width of wall 0.22
Width of drainage 0.025
Height of drainage 0.175
Property Measurement (m)
Outer diameter 1.97
Inner diameter 1.5
Thickness of wall 0.24
Height 0.52
Diameter of outlet 0.025
Diameter of shower 0.0875
Distance between tank 2.55
Circumference of tank 6.2
Property Measurement (m)
Outer diameter 1.15
Inner diameter 0.9
Thickness of wall 0.125
Height 0.425
Diameter of
outlet
0.025
✓ Capacity of Over Head Tank is
10,000 liter.
✓ Volume of Incubation Tub is 0.0006
(m3)

48. COLLECTION OF BROODERS FROM BROODER POND
Collection of Brooders with
Drag Net
Identification of Healthy Brooder

49. SEGREGATION OF MALE AND FEMALE
MALE FEMALE

50. HORMONAL ADMINISTRATION
Dose
Female- 0.15 ml/100g

51. Dissecting the Male Brooder
PREPARATION OF MILT SUSPENSION
Removal of Testis
Collected Testis

52. Grind the Testis with the help of
Pestle and Mortar
Preparation of Milt Suspension
with 0.9 % Saline Solution

53. Stitching of Dissected
Male Fish
After Stitching Treated with KMnO4

54. STRIPPING THE FEMALE BROODER
Gently Press the Belly and Egg Oozes Out Firmly mix with Sperm Suspension
Flow Through System Siphoning of Unfertilized Eggs

55. ORNAMENTAL FISH BREEDING AND CULTURE

56. s
]
WORKING PLACE
Glass Tanks
FRP Tanks
18
inch
24
inch
12
inch
Glass tank
1 m
1m
Circular FRP tank
12.7 m
6.4 m
Live Fish Laboratory
0.75
m.
0.5 m.
1 m.
Rectangular FRP tank

57. Measurement Of Wet Lab
S.No. PROPERTIES MEASUREMENT
1 Total number of aquarium tank 24
2 Total area of aquarium tank 4.32m2
3 Total volume of aquarium tank 1.96m3
Single aquarium tank
1 Length 0.6m
2 Width 0.3m
3 Depth 0.46m
4 Area 0.18m2
5 Volume 0.082m3
Glass Tanks
Sl. No. Properties Measurement
1. Total length of live fish laboratory 12.90m
2. Total width of live fish laboratory 6.36m
3. Total area of live fish laboratory 82.04m2
4. Primary area 67.08 m2
5. Secondary area 14.96 m2

58. S.no. PROPERTIES MEASUREMENT
1 Total number
of FRP tank
12 No.
2 Total area of
FRP tank
9.0m2
3 Total volume of
FRP tank
4.5m3
Single FRP tank
4 Area 0.75m2
5 Volume 0.375m3
S.no. PROPERTIES MEASUREMENT
1 Total number of
FRP tank
12
2 Total area of
FRP tank
9.36m2
3 Total volume of
FRP tank
9.36m3
Single FRP tank
4 Area 0.78 m2
5 Volume 0.78 m3
Circular FRP Tank:- Rectangular FRP Tank:-

59. Cleaning Of Glass tank Disinfection using KMnO4
Siphoning
Cleaning of FRP tank Water filling
Routine Maintenance

60. Feeding
Earthworm
Artificial feed
Earthworm Feeding
Infusoria Feeding

61. BREEDING OF LIVE BEARERS
Breeding of Black Molly
Brooders
Young once
Brooders
Young once
Breeding of Sword tail Breeding of Neon Platy
Brooders
Young once

62. BREEDING OF SHUBUNKIN
BREEDING OF EGG LAYERS
Young onesBreeding set
Sl. No. Ornamental Fish No. of Sets Young ones
1. Black molly 2 38
2. Sword tail 4 70
3. Mango platy 1 14
4. Neon platy 1 10
5. Shubunkin Gold fish 1 68
List of young ones produced from breeding of ornamental fishes

63. INFUSORIA CULTURE
Covering Tank with
net
Filling Clean water in
Tank
Inoculation of Banana
Peelings
Observation of Infusoria

64. Construction of Earthworm Earthworm Collection from wild Earthworm Culture Tank
Culture pits
Using Organic waste in Earthworm culture pits Earthworm
EARTHWORM CULTURE

65. PREPARATION AND MARKETING OF VALUE ADDED FISH
PRODUCT (FISH CUTLET)

66. INTRODUCTION
• VALUE ADDITION AND ITS IMPORTANCE
• Value addition is defined as “Any additional activity that in
one way or the other change the nature of product thus
adding to its value at the time of sale”. Value addition is
gaining more importance in our present days of change life
styles eating habits.
• Value addition and introduction of new types of products
from low cost fishes is the only solution to the problem.
Present market trends reflect a rapidly growing demand for
ready –to-cook and ready –to-serve convenience products.
Poor fisherman can earn more benefit from careful utilization
of the rich resources by value addition.

67. INGREDIENTS USED FOR PREPARATION OF FISH CUTLET
S.N. INGREDIENT QUANTITY
1 Fish 15 kg
2 Potato 7kg
3 Onion 4kg
4 Green chilly 500gm
5 Ginger 500gm
6 Curry leafs 150gm
7 Coriander powder 50gm
8 Chili powder 50gm
9 Turmeric powder 50gm
10 Masala 100gm
11 Eggs 24 no.
12 Wheat flour 500gm
13 Bread crumbs 1 packet
14 Salt 1kg
15 Vegetable oil 2lit.
16 Sauces 1packet

68. FLOW CHART OF FISH CUTLET
Take Raw Fish & proper washing
Filleting & washing
Washed fish get mixed with turmeric powder & salt
Boil the fish in hot water till it become colorless
After boiling cool it normal temperature
Remove bones & skin from fish meat
Mince the fish meat in fish mincer

69. Make a paste of all ingredients
Boil the potato & again get mixed together
Mince meat get mixed with all ingredient
Make small size round & flat shape
Dip in egg custard
Roll over the bread crumbs
Fry it into hot vegetable oil
Cool it & pack it
Cont…

70. PREPARATION OF FISH CUTLET
Dressing of Fish Filleting of Fish
Preparation of Ingredient Deboning of Fish
Frying of Ingredient
Making of Paste

71. Mixing of Minced Meat with Ingredients
Shaping of Cutlet
Shaping of Cutlet

72. Cutlet Ready for Fry Frying of Cutlet
Fish Cutlet ready to Eat Selling of Fish Cutlet

73. ECONOMICS OF FISH CUTLET
S.N. INGREDIENT QUANTITY PRICE (Rs.)
1 Fish 15 kg 2250
2 Potato 7kg 130
3 Onion 4kg 80
4 Green chilly 500gm 40
5 Ginger 500gm 50
6 Curry leafs 150gm 20
7 Coriander powder 50gm 50
8 Chilly powder 50gm 80
9 Turmeric powder 50gm 60
10 Masala 100gm 70
11 Eggs 24 no. 120
12 Wheat flour 500gm 50
13 Bread crumbs 1 packet 110
14 Salt 1kg 30
15 Vegetable oil 2lit. 240
16 Sauces 1packet 150
TOTAL 3580.00
MISCELLANEOUS
1. Paper plate 240
2. Gas 350
TOTAL (INPUT) 4170.00
SELLING OF CUTLET
TOTAL( OUTPUT) 5,980.00
PROFIT Output-Input
5980-4170
1810.00