1. BT 631: Protein Structure and Function (3-0-0-6)
Introduction to protein structure and function; amino acids: building blocks of proteins, peptide bonds,
polypeptides and its conformation; structural elements of protein structure: primary, secondary, tertiary
and quaternary structures; bonds stabilizing protein structure; structural motifs; methods of determining
three-dimensional structures of protein: X-ray crystallography, nuclear magnetic resonance (NMR), cryo-
electron microscopy; basic concepts in analyzing protein structures; protein structure database; structure
and function of fibrous proteins: (kerartins and collagens) and membrane proteins (ATPase family);
structures and mechanisms of dehydrogenases and proteases; chemical modification of enzymes using
affinity reagents and side-chain specific reagents.
Text Books/References
• Principles of Protein Structure, G.E. Schulz and R.H. Schirmer, Springer (1979).
• Proteins: Structure and Molecular Properties, Thomas E. Creighton, W.H. Freeman (1992).
• Introduction to Protein Structure, Carl Branden and John Tooze, Garland Science (1999).
• Proteins and Enzymes, JE Bell and ET Bell, Prentice Hall College Div (1988).
• Proteins: Structure and Function, David Whitford, John Wiley (2005).
• Reviews and Articles.
Course Syllabus

2. S. No. Date/Day Topic to be covered
1. 08.08.2013 Introduction to the course
2. 12.08.2013 Introduction to protein structure and function
3. 13.08.2013 Amino acids
4. 19.08.2013 Peptide bonds
5. 22.08.2013 Polypeptide and its conformations
6. 26.08.2013 Structural elements of protein structure
7. 27.08.2013 ,,
8. 29.08.2013 Bonds stabilizing protein structure
9. 02.09.2013 ,,
10. 03.09.2013 Structural motifs
11. 05.09.2013 ,,
12. 09.09.2013 X-ray crystallography
13. 10.09.2013 ,,
14. 12.09.2013 ,,
15. 16.09.2013 ,,
16. 17.09.2013 NMR
Tentative Class Distribution

3. S. No. Date/Day Topic to be covered
17. 19.09.2013 ,,
18. 23.09.2013 Mid Sem Exam
19. 24.09.2013 ,,
20. 25.09.2013 ,,
21. 26.09.2013 ,,
22. 27.09.2013 ,,
23. 28.09.2013 ,,
24. 30.09.2013 Cryo EM
25. 01.10.2013 ,,
26. 03.10.2013 Basic concepts in analyzing protein structures
27. 07.10.2013 ,,
28. 08.10.2013 Protein structure database
29. 10.10.2013 Structure and function of fibrous proteins
30. 17.10.2013 Keratins
31. 21.10.2013 ,,
32. 22.10.2013 Collagens
33. 24.10.2013 ,,

4. S. No. Date/Day Topic to be covered
34. 28.10.2013 Membrane proteins
35. 29.10.2013 ,,
36. 31.10.2013 ATPase family
37. 04.11.2013 ,,
38. 05.11.2013 Structures and mechanisms of dehydrogenases
39. 07.11.2013 ,,
40. 11.11.2013 Structures and mechanisms of proteases
41. 12.11.2013 ,,
42. 18.11.2013 Chemical modifications
43. 19.11.2013 Using affinity reagents
44. 21.11.2013 Side chain specific reagents
45. 25.11.2013 End Sem Exam
46. 26.11.2013 ,,
47. 27.11.2013 ,,
48. 28.11.2013 ,,
49. 29.11.2013 ,,
50. 30.11.2013 ,,

5. Total No. of Classes available: 38 + 2 (Quizzes)
Quiz No. 1 (10 Marks): on 04.09.2013 (Wednesday Time=4-5PM
Mid Sem Examination: 40 Marks
Quiz No. 2 (10 Marks): on 26.10.2013 (Saturday) Time=10-11AM
End Sem Examination: 40 Marks
Total Marks: 100
Marks Distribution