1. RESEARCH PROFESSIONAL
Seeking a position in an organization that provides me ample opportunity to explore & excel while
carving out the niche for personal, professional as well as organizational goals
PROFILE
 Result oriented and competent professional with 7.4 years of experience in the field of
Research and Development; spearheading efforts as Senior Research Fellow with
Anthropological Survey of India.
 Well versed withjFully Auto-analyzer EM360 (TRANSASIA) for Bio-chemical test of Blood (Serum
/ Plasma) and Urine, which provide unique platform to make appropriate application of the
instruments for Molecular genetics research.
 Adept at Genomic DNA extraction / isolation, quantification (Human); PCR based mutation
detection especially of disease genes, Haemoglobin (lysate) electrophoresis (cellulose acetate
membrane), Blood grouping, Complete blood count (CBC); High performance liquid
chromatography (HPLC, Bio-Rad).
 Proficient in techniques pertaining to instruments like - Electronic digital balance, Digital pH
meter, Shaker water bath, Digital autoclave, Incubator, UV Spectrophotometer, Ultra speed
centrifuge, Electrophoresis, Electronic Cell counter, HPLC, PCR- RFLP & Sequencing .
 A systematic, organized, hardworking & dedicated team player with an analytical bent of mind
& quick problem solving ability; excellent communication and interpersonal skills.
WORK HISTORY
ANTHROPOLOGICAL SURVEY OF INDIA, since Feb ‘12
Biological Anthropology (DNA Laboratory)
Senior Research Fellow
 Spearheading efforts as Senior Research Fellow; completed the DNA sequencing, processing,
analysis and mutation detection of around 800 DNA samples for 5 genes using the DNA
sequencing instrument 3730 DNA analyzer.
 Research work on Phylogeny study of Indian populations (around 36 communities) with the
technology of whole genome sequencing of mtDNA and DNA fingerprinting of Y-Chromosome
as a team member of the project ‘DNA polymorphism of the Contemporary Indian
Populations’.
 Responsible for conducting research activities of the project, initialization of research
proposals, finalization of research proposals.
 Prepare Specifications, Technical summary reports, validation summary reports, analytic data
review and Technology transfer protocols & printing of extension manuals and reports.
 Performed studies as related to research project & perform & conduct various field
experiment/management with different treatments as required by the project.
 Providing insight on these findings and ensures key findings are shared with appropriate
internal
 Identifying issues and forming hypotheses, formulating and implementing
recommendations/solutions.
Junior Research Fellow, Aug ‘08-Feb ‘12
 Responsible for carrying out research and data collection; conducting analysis to determine
project’s technical and strategic needs.
 Analyzed, summarized and presented results for all research projects. Regularly reviewed
ongoing research for trends.
 Coordinated research activities between various faculties, performed and conducted various
field experiments/management with different treatments as required by the projects.
PULAKES PURKAIT
Anthropological Survey of India, Western Regional Centre, Pratap Nagar, Udaipur-313001-Rajasthan
Contact: +919929515472, Email: pulakes.purkait28@gmail.com ; pp.diabetes@gmail.com
SKILLS
Molecular Techniques
Molecular Anthropology
A Genetic Study OF
Diabetic Nephropathy
among the Bengali
Population, India with
special reference to
Renin-Angiotensin-
Aldosterone System
(RAAS) Gene
Polymorphism
Research
Methodology Course
in Anthropological
and Allied Sciences
(2
nd
– 11
th
December
2013).
Audio and Digital
Video Editing and
mixing and
Processing, Software
like: Pinnacle studio-
9; Windows Movie
Maker, etc
UTA Phase II STAGE
–II Training of Peer
Educations &
Community Leader
organized by NSS
Cell, Vidyasagar
University.

2. DNA polymorphism of the Contemporary Indian Populations
The Anthropological Survey of India Study of Bio-Cultural Risk Factor Assessment for Type 2
Diabetes: Consortium for the Family Studies of Genetics of Type 2 Diabetes in Indian
Populations
12
th
Five Years Plan Project “Bio-cultural Diversity, Environment and Sustainable Development
A Genetic Study of Diabetic Nephropathy among the Indian Populations with special reference
to Renin-Angiotensin - Aldosterone System (RAAS) Gene Polymorphism
Seminars/Conferences Attended
Presented a paper on Metabolic syndrome and age in the National seminar on Status of Senior
Citizens Issues and Challenges, Udaipur 2013
Presented a paper on Null association of aldosterone Synthase (CYP11B2) gene – 344 C > T promoter
polymorphism intype2 diabetic patients among Indian populationin 3
rd
WorldCongress on Diabetes &
Metabolism 2012
Participated and presented a poster on Association of Angiotensin Converting Enzyme (ACE)
gene Insertion / Deletion polymorphism and Type 2 Diabetic Nephropathy in Eastern Indian
Population in the International Conference on 4th
Diabetes India International Conference
2011, Delhi
Participated and presented a paper on A Study on Anthropometric Characteristics and
Nutritional Status of Adult Dhimals: An Isolated Indigenous group of West Bengal in the “UGC
SAP National Seminar on Tribal Development in India: Challenges and Interventions”
Presented a poster on ACE gene polymorphism in Eastern Indian Diabetic Nephropathy
Patients" in the International Conference on Multidisciplinary Approaches to Diabetes
Research & Health (ICMADRH – 2010) Jaipur
Participated in the III rd National Conference on New Frontiers in Haematology & Oncology
Participated in the “ISHG 2009 – International Symposium on ‘Ethics, Culture and Population
Genomics’ & 34th
Annual Conference of the Indian Society of Human Genetics from March 17
to 20, 2009 at New Delhi and delivered an invited Poster titled “Undernutrition and Association
of ABO Blood Groups with Haemoglobin Level among the Adult Dhimals of Naxalbari, West Bengal,
India –A Review.”Poster No. 10.
Participated in the National Conference on Community Genetics Approaches in Prevention of
Beta Thalassemia from 7
th
to 9
th
March 2008 at Kolkata
Achievement:
Identified 5 new mutations for type 2 diabetes and type 2 diabetic nephropathy patients of Bengali
population, all 5 mutations were accepted by NCBI gene bank
NCBI_ss#RENIN_ns01_Promoter_Pulakes 825678878
NCBI_ss# RENIN_ns02_Promoter_Pulakes 825678996
NCBI_ss# RENIN_ns03_Promoter_Pulakes 825678997
NCBI_ss#RENIN_rs04_Promoter_Pulakes 784304486
NCBI_ss#RENIN_ns05_Promoter_Pulakes 825678872
GSTM1 Null Genotype Associated With Type 2 Diabetic Nephropathy Patients among Indian
population. WJPR 3(3):4452-4463.
Association of Lifestyle variables with the Novel Mutation of BRCA1 gene in Breast Cancer: A
Case control Study among the Hindu females of West Bengal, WJPPS 3(6): 1213-1226.
Analysis of Aldosterone Synthase Gene Promoter (- 344 C >T) Polymorphism in Indian Diabetic
Nephropathy Patients. J Diabetes Metab 4: 271.
Renin- Angiotensin- Aldosterone System gene polymorphisms in Type 2 Diabetic patients
among the Mewari population of Rajasthan. International Journal of Biological & Medical
Research, 4(2): 3128-3134.
ACE Gene Insertion / Deletion Polymorphism and Type-2 Diabetic Nephropathy in Eastern
Indian Population. Human Biology Review (ISSN 2277 4424) 2 (1) pp 66 -76.
Gender based association between ACE gene (I/D) polymorphism and Type 2 Diabetic Mewari
patients of Udaipur city, Rajasthan. Jr. Anth. Survey of India, 62(2):169-177.
Technical Skills
Bioinformatics: SeqScap,
Bioedit, Net primer, NCBI
blast, PLINK and
HAPLOVIEW software for
Genetic analysis
Statistics: SPSS 16,
MINITAB 11, gplink
Software for clinical &
genetic data analysis
Internet Applications
EDUCATIONAL
CREDENTIALS
Ph. D
M. Sc (Anthropology)
2006

3. Metabolic Syndrome and age: a study among the Saharia tribal community of Rajasthan in
proceeding of National Seminar on Status of Senior Citizens Issues and Challenges, December
22-23, Book Pp: 29-32
Prameha and its Ancient Ayurvedic medicine in India. Jr. Anth. Survey of India, (2012-2013) 61 (
2):659-669
Sexual Dimorphism in Health and Nutritional Status of Three Communities of Darjeeling
District in West Bengal and Comparison with Some Other Populations of Eastern India – An
Anthropometric Appraisal. Journal of LifeSciences1 (1): 27-34.
Age-Sex Variation and Association of OAB Blood Group with Haemoglobin Level among the
Adult Dhimals at Naxalbari in West Bengal. Anthropologischer Anzeiger . Jg. 66 Stuttgart,
December 2008; Volume: 66, Issue: 4, Pages: 379-384.
Under nutrition among adult Dhimal of Naxalbari, WB: Comparison with other tribes of Eastern
India. Food and Nutrition Bulletin, Vol. 28 (3) 348-352 the United Nation University.
Gender based Association of CYP11B2 (-344 C/T) Polymorphism in Indian Type 2 Diabetic
Patients. WJPR. (Accepted)
Association between the Polymorphism of The Angiotensin- Converting Enzyme Gene and
Breast Cancer Risk among The Bengali Hindu Females of West Bengal, Anthropologischer
Anzeiger (Accepted)
The Vedic Culture: Welfare and Women Jr. Anth. Survey of India (Accepted)
A comparative study of Angiotensin Converting Enzyme (ACE) Gene Polymorphism of Bengali
and Mewari Type 2 Diabetes Patients in India. Journal of Harmonized Research in Pharmacy
(Accepted)
 Null association of aldosterone Synthase (CYP11B2) gene – 344 C > T promoter polymorphism in type2
diabetic patients amongIndian population.JDiabetes Metab, 2012,3:8 pp74
Association of Angiotensin Converting Enzyme (ACE) gene Insertion / Deletion polymorphism
and Type 2 Diabetic Nephropathy in Eastern Indian Population. 4
th
Diabetes India International
Conference 2011 Abstracts. Poster No. 1, Book.Pp 45.
Undernutrition and Association of ABO Blood Groups with Haemoglobin Level Among The Adult
Dhimals Of Naxalbari, West Bengal, India – A Review. ISHG 2009 Programme and Abstract Book.
Poster No. 10. pp 90.
Visited the Village “SULTANA” of Jaisalmer district in Rajasthan for “Border area study of Desert
village” on Socio economic status, Sustainable development and Health.
Attended the Health Awareness and Screening Camp among the Garasia Tribe of Rajasthan
along with team Under the project ‘Community Genetics & Health: Bio-cultural Adaptation’
Visited the Village “Ranawatan-ki-Sadri” Chittorgarh district in Rajasthan for restudy of the
village after 50 years on Socio economic status, Sustainable development and Health supported
by Anthropological Survey of India, Govt. Of India.
Successfully taken a Health Awareness Camp and collected the blood samples among the
SAHARIA primitive tribal group in Baran district of Rajasthan.
Carried out a Case control study in Udaipur city of Rajasthan under the project titled “ A Genetic
Study of Diabetic Nephropathy among the Indian Populations: With Special Reference to Renin-
Angiotensin – Aldosterone System (RAAS) Gene Polymorphism”
Carried out a cross sectional as well as Case Control study in Kolkata city for the project titled
“A Genetic Study of Diabetic Nephropathy among the Indian Populations: With Special
Reference to Renin- Angiotensin – Aldosterone System (RAAS) Gene Polymorphism”.
Worked among Mathur Community in Jodhpur City and Udaipur city of Rajasthan for study of “
The Anthropological Survey of India Study of Bio-Cultural Risk Factor Assessment for Type 2
Diabetes : Consortium for the Family Studies of Genetics of Type 2 Diabetes in Indian
Populations” under Anthropological Survey of India.
Worked among the Dhimals of Naxalbari, Darjeeling, West Bengal while working on “A Study
on Anthropometric Characteristics and Nutritional Status of Adult DHIMALS of Naxalbari
Worked among the Sabars of Keonjhar district, Orissa and prepared a field report for M.Sc.
Part-I Examination.
Study on socio economic status on a Multi-ethnic village in Purbi Singhbhum district of
Jharkhand and prepared a field report for B.Sc. (Hons.) Examination.
Date of Birth: 28
th
November 1983
Languages Known: English, Hindi and Bengali
Permanent Address: Vill+Post: Durganagar, Via+PS: Dholahat, Dist.: South 24 Parganas, WB
References: Available on Request