1. LORRAINE SUHADOLNIK
(215) 885-3947 (Home)
3127 Jenkintown Road (267) 546-6724 (Cell)
North Hills, PA 19038 lorrainesuhadolnik@yahoo.com
SUMMARY
Research Scientist with extensive drug discovery experience in the pharmaceutical industry. Diverse skill set that allows
flexibility and adaptability to excel in all therapeutic research areas. Strong technology background in molecular biology,
protein biochemistry, assay development, screening and tissue culture. Excellent communicator and accomplished team
member in a matrix environment.
TECHNICAL TRAINING
Assay Development: GPCR Assays, HitHunter Assays, Enzymatic Reactions, Transfections, Cell Based Assays, SPA
Assays, FRET Assays, Optimize assays for high-medium throughput, Troubleshoot assays
Screening: Low to medium throughput screening, Compound ordering, Chemistry liaison, Data analysis, Database
Recording, Teecan, Transacta Robotic Diluter
Molecular Biology: Transcriptional Assays, Signal Transduction Assays, Transcription-Translation Assays, Yeast and
Mammalian Two-Hybrid Systems, PCR/ RT-PCR/Taqman, Micro array gene expression modeling, Restriction digest/mapping,
Cloning techniques, DNA/RNA techniques
Protein: Protein production and purification, ImmunoPrecipitations, Antibody Purification, Westerns, ELISA Assays, Gel Shifts
Tissue Culture: Mammalian and Insect Cell Culture including routine maintenance, Infections-Baculovirus/Adenovirus,
Transfections, Cytotoxicity Assays, Electroporation, Primary Cultures In Vivo
SOP: Accountable for recording progress in notebooks, presenting data at meetings, coordinating lab responsibilities,
literature searching for new assays and information to aid in the advancement of projects.
Communication Attributes: Productive Interaction with other groups, PowerPoint Presentations at inter and intra-
departmental meetings, Poster Presentations, Manage laboratory organization and activities, Facilitate activities for company
events, Facilitate activities for department events
Computer Skills: Microsoft Office: Excel, PowerPoint, Word, Statistics Data Bases, GraphPad Prism, Sequence Applications
(Alignments; Blast; Translational)
PROFESSIONAL EXPERIENCE
Teacher’s Assistant/Volunteer/Tutor 2009-Present
Utilize science and math background to tutor and mentor sixth through twelfth grade students. Provide personalized instruction
for ACT, PSAT and SAT preparation. Prepare and edit resumes for summer interns, college graduates and professionals.
WYETH RESEARCH, Collegeville, PA 1995- 2008
Research Scientist II, Women's Health and Musculoskeletal Biology (2000-2008)
Developed and executed in vitro functional assays for fast track projects under exacting and critical timelines. Emphasis on
advancing projects, either independently or as part of a team. Developed and validated new assays related to receptor
function and the effects of hormone treatments. Introduced and validated yeast-based assays, mammalian 2- hybrid systems
as well as the gene expression by baculovirus and adenovirus systems.
•Developed primary transfection screens for the chemical screening group, followed up with secondary
screens to analyze the compounds obtained from a high throughput screen.
• Focused on steroid receptor function, hormone replacement therapy, contraception and reproductive endocrinology.
Developed biochemical conversion assays to monitor neuronal transmission in the brain specifically as related to
enzymatic reactions effecting female sexual dysfunction. Generated, analyzed and communicated results to team
members. Project status was advanced on development track months before the scheduled completion date.
• Developed dual-transfection cell based assays to directly target the interaction of androgen receptor (AR) and nuclear
receptor co-repressor (NCoR) to develop non-steroidal selective AR modulators for the treatment of prostate cancer and
male fertility. The AR/NCoR project was advanced to the next stage of development several months before the targeted
completion date.
• Supervised junior staff members on various molecular biology techniques, assay development, efficient execution of
experiments and analytical review of data. Directed and coached junior scientist on the intricacies of assay stability,
method development, trouble-shooting and validation. Instructed on usage of analytical programs for data interpretation.

2. PAGE 2 LORRAINE SUHADOLNIK
(215) 885-3947
Research Scientist I, Women’s Heath Research Institute (1997-2000)
Conducted research on nuclear receptors supporting the evaluation of potential targets by analyzing molecular mechanisms
involved in tissue-specific regulation. Characterization of targets and compounds obtained from HTS were determined by
ELISA, gel shift, Western and cell-based transfection assays.
• Developed primary assay for meiotic gene expression as related to male fertility. Focus on development of a non-
hormonal drug that would disrupt the meiotic DNA break repair of the MSH5 gene for the purpose of male contraception.
Assisted in the protein sequence design for antibody production. Isolated, purified and tested the MSH antibodies for
reactivity.
• Performed qPCR analysis of gene regulation in phases of meiosis. Used tissue specific mRNA obtained from mice at
different stages of development. Analyzed data representing the progression of genes as they were up-regulated and
down-regulated in different meiotic phases.
• Provided support for yeast, insect and mammalian cell-based assays including subsequent analysis of data and
submission of data for company database protocols.
Senior Molecular Biologist, Women’s Health Research Institute (1995-1997)
Supported the research of three senior scientists: maintained and supplied necessary tissue culture, performed transient
transfections and treated appropriately, generated baculovirus as needed, and analyzed and presented data with future
experimental focus.
•Characterized cell lines with regard to estrogen receptor in order to identify and profile candidate compounds.
• Analyzed the estrogenic response of candidate “hot flush” genes by developing second-generation gene transcription
assays to profile candidate compounds. Genes were analyzed using molecular biology techniques and results presented
to the group along with follow-up experimental plans.
EDUCATION
BA, Chemistry, Temple University, School of Arts and Sciences, Philadelphia, PA
Profile Website
http:/www.linkedIn.com/in/lorrainesuhadolnik
TECHNICAL ADDENDUM
PUBLICATIONS

3. PAGE 3 LORRAINE SUHADOLNIK
(215) 885-3947
Panayiotis Stevis, Darlene C. Deecher, Lorraine Suhadolnik. Larry M. Mallis and Donald Frail, "Differential Effects of
Estradiol and Estradiol-BSA Conjugates'" Endocrinology 140. 5455-5458 (1999)
K. Kariko, R. W .Sobol, Jr., L. Suhadolnik. N.L.Reichenbach, R.J. Suhadolnik, I and W. Pfleiderer, "Phosphorothioate
Analogues of 2', 5'-Oligoadenylate. Enzymattically Synthesized 2',5'-Phosphorothioate Dimer and Trimer: Unequivocal
Structural Assignment and Activation of RNase L", Biochemistrv 26 7127-7135 (1987).
K. Karilo, S.W. Li, R.W. Sobol, Jr., L. Suhadolnik. N.L.Reichenbach, R.J. Suhadolnik, R. Charubala and W. Pfleiderer,
"Phosphorothioate Analogues of 2',5'A: Elucidation of the Stereochemical Course of the Enzymes of the 2-5A
Synthetase/RNAse L System", Nucleosides & Nucleotides 6, 173-184(1987).
ABSTRACTS
Alfinito P.D., Suhadolnik L., Cosmi S., Chen X., Stephens C., Pawlyk A., Winneker R., Deecher D., Neuronal Nitric Oxide
Synthase is a Novel Target for the Development of Nonhormonal Treatments for Vasomotor Symptoms Associated with
Menopause [Abstract] WHMSB Retreat 2007.
Chengalvala M, Suhadolnik L., Shanno L., Kopf G. Interaction of the Androgen Receptor and Nuclear Receptor Corepressor
as a Target for Developing Selective Androgen Receptor Modulators. [Abstract] Wyeth Discovery Retreat 2006.
Shen E, Bapat A, Chen G, Chengalvala M, Chennathukuzhi V, Hoffman W, Kopf GS, Shanno L, Stevis P, Suhadolnik L,
Oocyte-specific BMP Family Members, GDF9 and BMP15: Potential Targets for Female Contraception. [Abstract] Discovery
Retreat 2005.
Samuel M, Lane M, Suhadolnik L, Merchenthaler I, Kopf G, Stevis P. Calbindin-d9k expression is regulated by estrogen in
the hypothalamus of the rat: close association with estrogen receptor-alpha (ERalpha). In vivo confirmation of microarray data
utilizing an immortalized cell line (D12). [Abstract] Endocrine Society Annual Meeting, Boston, MA, Jun 24, 2006.
Suhadolnik L, Stevis PE. Activation of multiple signaling pathways by estrogen receptor alpha and beta in the presence of
antagonist. [Poster Session] 86th Annual Meeting of the Endocrine Society, New Orleans, LA, Jun 12-18, 2004. UI: 7749.
Zhang Z, Lundeen SG, Zhu V, Carver JM, Suhadolnik L. Stevis P.,Winneker RC,"ln-Vitro Characterization of trimegestone: A
New Potent and Selective Progestin"Progesterone, Progestines And Antiprogestines, Jerusalem (1999).
H. R. Hubbell, K, Kariko, R.W. Sobol, Jr., S. Pombanlualap, S.W. Li, L. Suhadolnik. N.L. Reichenbach D.H. Gillespie, W.A.
Carter, I. Brodsky, p. Sheets and R.J. Suhadolnik, "Human Heterogeneous Nuclear RNA-Activated 2-5A Synthetase
Produces Biologically Active 2-5A", Fed. Proc. 46. 2014 (abstract 515) (1987).
K. Kariko, R.W.Sobol, Jr., L. Suhadolnik. N.L.Reichenbach, R.J. Suhadolnik, R. Charubala and W. Pfleiderer,
"Phosphorothioate Analogs of 2-5A: Activation of RNase L by Trimer Cores and 5'-Monophosphates'" Fed. Proc. 46. 2083
(abstract 922) (1987).