here you will get about antigene and antisense gene therapy

1. ANTIGENE AND ANTISENSE GENE
THERAPY
PRESENTED BY
MISS. P.G. PURANIK
M.SC. (ZOOLOGY) B.ED, PH.D PURSUING
SHRI SHIVAJI SCIENCE COLLEGE AMRAVATI

2. Contents
• Introduction
• Methods
• Advantages and limitation
• References

3. Antigene and Antisense Therapy
• Blocking transcription using a single stranded nucleotide sequence (
antigene oligonucleotide) that hybridizes with specific gene, this is
called as Antigene therapy.
• Antisense Therapy refers to the Inhibition of translation by using a
single stranded nucleotide ( Antisense oligonucleotide)
• It also possible to inhibit transcription and translation by blocking
with oligonucleaotide the transcription factor responsible for the
specific gene expression.

4. • Nucleic acid therapy refers to use of DNA or RNA molecules for
therapeutic purpose,

8. Antisense therapy for Cancer
• Oncogene :
• Antisense oligonucleotide are use for the treatment of myeloid
leukemia in 1991.
• This approach is effective only if the antisense oligonucleotide
specifically bind to target mRNA and block protein biosynthesis.
• This can achieved in two ways illustrated in Next slide.
•

10. Anti sense therapy for HIV
The target cell for HIV infection are genetically
engineered to contain a gene that can express a
complementary copy of HIV genome.
The gene produces antisense RNA
Cell containing antisense RNA infected by HIV
Antisense RNA bind to viral genome (RNA)
For RNA-RNA hybrid molecules
Double stranded molecules cannot be used by
enzymes Reverse transcriptase

13. Advantages of Gene therapy
• It is permanent solution for genetic diseases.
• It is possible to cure Cancer, HIV
• It also helps to eradicated genetically related disease

14. limitations
• Due to some limitation gene therapy has not progressed the way it
should ,despite intensive research.
• Break though may come anytime and of course this is only possible
with the persistent research.
• It might take some year.

15. References
1. https://www.google.com/search?q=anti+gene+therapy
2. https://www.slideshare.net/pooranachithraflowry/antisense-therapy-48602109
3. Satyanarayana U. [2005 ] Biotechnology, BOOKS AND ALLIAED (P) lTD . Kolkata: pp 169-172, ISBN no:8687634-90-9.