This document provides a simple roadmap to crack the microbiology and biotechnology section of the GPAT exam in 5 phases. Phase 1 focuses on vaccines and immunology. Phase 2 covers sterilization techniques and staining methods. Phase 3 discusses hybridoma technology, blotting techniques, and diagnostic tests. Phase 4 involves recombinant DNA technology including vectors and PCR. The roadmap is designed to efficiently cover the essential topics in a step-by-step manner to help students pass the exam.