Agrobacterium mediated gene transfer is a method of genetically transforming plants. Agrobacterium tumefaciens causes crown gall disease in plants by transferring oncogenic T-DNA from its Ti plasmid into the plant genome. The process begins when wounded plant cells release phenolic compounds that are recognized by A. tumefaciens. This triggers the cleavage and stabilization of T-DNA by virulence genes, followed by its transfer into the plant nucleus through a type IV secretion system encoded by other virulence genes. Once integrated, the T-DNA causes tumors to form and benefits A. tumefaciens by altering plant cell physiology.

1. AGROBACTERIUM
MEDIATED GENE
TRANSFER !!

2. METHODS OF GENE TRANSFER IN PLANTS

3. Agrobacterium mediated gene transfer!!
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4. Crown gal disease Hairy root disease

5. A. Tumeifaciens

6. Mechanism of gene transfer

7. How Agrobacterium induces Tumor??
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8. Why do A.Tumeifaciens infects plants ??
What benefit it will get from plant??
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9. Functions of

10. “Sites in virulence region” of Ti-plasmid

11. How A.Tumeifaciens transfers
its Genetic material to plant??

12. 1.Wounded plant will release the
Phenolic compound

13. 2.Recognizion by A.Tumeifaciens
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V-region

14. 3.Cleaving of T-DNA in Ti plasmid
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virD1
virD2
ssDNA

15. 4.Cleaved TDNA stabilizing
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virD2
Cleaved
TDNA
virE

16. 5.Transfer of TDNA into plant nuclear
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Vir B & virD4

17. 6. Induction of tumor
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18. Functions of “Virulence-genes”!!

20. How A.Tumeifaciens will carry our gene of
interest ??
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