Rosehip extracts were tested on A-172 human glioblastoma (GBM) cell lines to determine their effects on cell death. The cell lines were treated with 250 μg/ml of rosehip extracts for 48 hours. As a positive control, some cells were also treated with staurosporine. The amount of cell death was measured using a live/dead colorimetric assay. The results showed that exposure to rosehip extracts did not result in cell death of the GBM cell lines.
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Fi g. 6. R osehip extracts do not promo te cell d eath .
Fig. 6 . Ex posure to Ros ehip extr acts does not resul t in cell death . A-17 2 huma n GBM c el l lines w ere tr eate d
with rosehip extr acts for 4 8 h at 250? /ml. In additi on to r ose hip ex trac ts, st au rosporine (1? ) was also used.
The a mount of cell death was dete rm ined us ing a Live/Dead c olori metirc assay.
BF
Viabl e
cells
No n-Viable
cells
+ S erum + R H P 250 µg/ m l + S ta u rospo rin e 1µ M
(a) (d) (g)
BF
(b) (e) (h)
Viable
cells
(c) (f) (i)
Non-Viable
cells
+ Serum + RHP 250 μg/mL + Staurosporine 1 μM