Here you will get to know about Drug discovery process and Target identification and validation.

1. Target Discovery
And Validation
Presented by:-
Shaikh Nisar Ali (M.Pharm)
HKE’S MTRIPS.
Department of Pharmacology
Under the Guidance of:-
Mrs.Arati Malpani(Ph.D)
8 October 2021

2. ROLE OF :-
• Genomics
• Nucleic Acid Microarray
• Proteomics
• Protein Microarray
• Bioinformatics
• Antisense Technologies
• Antisense Oligonucliotides
• Zinc Finger Protien
• Role of transgenic animals in target Validation

18. SiRNA/Gene Silencing RNA/Ninja RNA
• siRNA (short [or small] interfering RNA) is a class of small double-stranded
RNA molecules that silence gene expression by inducing the RISC (RNA-
induced silencing complex) to cleave mRNA.
• Introduced by 2 ways:-
1. By Researchers
2. By certain Virus that produce Antisense RNA.

28. Antisense Technology
• It is possible to synthesise a strand of nucleic acid that binds with
mRNA and inhibits the production of protein.
• Used to treat disease , when genetic sequence of particular gene is
known to be causative for particular disease.

29. Rnase H

33. Zinc Finger Protein.
• Zinc finger protein are among the most abundant protein in a
eukaryotic genomes.
• They present in tandem repeat.
• Zinc ion holding the DNA folds.
• Largest known DNA-binding family in multicellular organism.
• composed of two beta layer and one Alpha helix.
• 2 Cysteine and 2 Histidine Residue located in certain position binding
zinc to stabilize the structure.

35. Zinc finger Nucleases.
• Zinc finger nucleases (ZFNs) are a class of engineered DNA-
binding proteins that facilitate targeted editing of the genome by
creating double-strand breaks in DNA at user-specified
locations.
• Help in Genome Editing.

45. Thank You
8 October 2021