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DENTIN
Introduction
• Dentin is the mineralised tissue
that forms the bulk of the tooth
• It is present in the crown and in
the root portion of the tooth.
• It is covered by enamel in the
crown portion and cementum in
the root portion.
• It consists of mineralized
collagen matrix consisting of
closely packed dentinal tubules
which extends the entire
thickness of the dentin.
Physical
properties of
dentin
• Dentin is pale yellow contributes the color of
the tooth
• Dentin is harder than the bone and cementum
but softer than the enamel.
• Even though the dentin is rigid ,it is an elastic in
nature ,which permits slight deformation.
• The tubular structure of dentin makes it
permeable ;this permeability , however
decreases with increasing age.
Chemical
properties of
dentin
• 70% of inorganic material and 20% of
organic material and 10% water
• Inorganic portion –calcium hydroxy apetite
crystal
• Organic portion – collagen fibres mainly type
I collagen.
• Ground substance- non collagenous matrix
protein.
Histology of dentin
1. DENTINAL TUBULES
Odontoblast forms the dentin
matrix and move towards the
pulp.
shape of dentinal
tubules
• The thickness of dentin ranges from 3 to 10 mm.
• Buccal and lingual surfaces are bigger than the mesial and
distal surfaces.
• The tubules are closely packed towards pulp and widely
packed dentinoenamel junction.
• Dentinal tubules contains terminal branches and lateral
branches
Lateral and
terminal
branches of
dentinal
tubules
Predentin
• Predentin is the newly laid ,yet
to be mineralized dentin matrix.
• It is the innermost layer of the
dentin clos to the pulp.
• Width is 6 to 10micrometer.
• It becomes a part of mineralized
dentin when it starts undergoing
mineralization.
Peritubular dentin
• The dentin that present immediately
next to the dentinal tubules is the
peritubular dentin.
• The minerals are deposited in the
inner wall of tubule for the formation
of peritubular dentin so it is called as
INTRATUBULAR DENTIN.
• The thickness of peritubular dentin is
0.75micrometer in outer dentin and
0.4micrometer.
• The thick organic matter rich in
glycosaminoglycans called lamina
lamitants is seen in peri tubular dentin
Intertubular
dentin
• The dentin situated between the dentinal tubules is the intertubular
dentin which forms the major bulk of the dentin
• It is made up of type I collagen fibres.
Odontoblastic
process
• Odontoblastic process are the
cytoplasmic extensions of the
odontoblast which extend into
the dentinal tubules
• The process has a diameter of 3-
4 micrometer when it enters the
dentinal tubules and tapers to
1micrometer as it extends
further into the tubule.
Odontoblastic process
Primary dentin
• MANTLE DENTIN
Mantle dentin is a first formed dentin in
the crown.
It is less mineralised
It is composed of larger collagen fibre
It shows branching of dentinal tubules.
Circumpulpal
dentin
• Circumpulpal dentin constitutes
the remaining primary dentin
• When compared to mantle
dentin , circumpulpal dentin is
more mineralised and has
collagen fibres
Interglobular dentin
• During the mineralisation of dentin matrix the
minerals are deposited as globules
• In most areas these globules fuse to form a
uniformly calcified tissue .
• When some of the globules fails to fuse into a
homogenous mass hypomineralised area will form,
called as interglobular dentin.
Age and functional changes
• Secondary dentin
• Transluscent dentin
• Tertiary dentin
• Sclerotic dentin
• Dead tracts
Secondary Dentin
• Dentin formed after root completion
• Borders the pulp
• Formed by continuous , slow deposition of
dentin by odontoblasts
• Fewer dentinal tubules
• Function : Continuous deposition leads to
smaller pulp chamber and narrow root
canals in old patients
• Protects the pulp from exposure
Translucent dentin
• As a result of physiologic ageing, dentinal
tubules become completely occluded with
apatite crystals
• Common in the root
• Same refractive index as intertubular dentin
• Occluded dentinal tubules appear translucent
in ground section
• Formation starts apically, proceeds cervically
with age.
• Function: Used in Forensic dentistry for age
estimation
Tertiary dentin
• Dentin formed in response to various stimuli like attrition, caries or
restorative dental procedure
• Types
• Reactionary dentin
• Reparative dentin/Osteodentin
Reactionary dentin
• Formed by existing odontoblasts
which have survived severe
damage cause to them by
stimulus.
• Irregular appearance with fewer
tubules
Reparative dentin
• Formed by newly differentiated
odontoblast-like cells that replace
the original odontoblasts that
have been destroyed by stimulus
• Newly differentiated odontoblast
like cells arise from deeper regions
of the pulp
• Irregular with fewer , more
twisted tubules
• Newly differentiated odontoblast
like cells get entrapped in the
matrix they form to produce
osteodentin.
Possible origin of newly differentiated
odontoblast like cells
• Dormant odontoblast cells formed as a result of epithelial-
mesenchymal interaction during tooth development
• Odontoblast like cells that arise from stem cell population,without
epithelial-mesenchymal interaction, under the influence of cytokines
and growth factors
Sclerotic dentin
• Filling up of dentinal tubules with calcified
material in response to caries, attrition, erosion
• Defensive reaction
• Keeps the pulp vital for a longer time
• Fine meshwork of apatite crystals
• Transparent
• Appears light in transmitted light and dark in
reflected light.
• Mineral concentration is higher, crystal size is
smaller
• Harder
Dead tracts
• Empty dentinal tubules due to
retraction of odontoblastic
process or death of odontoblast.
• Occurs as a result of caries,
attrition, erosion
• In ground sections, appear dark
in transmitted light and white in
reflected light
• Commonly seen in narrow pulp
horns
• Common in older teeth
DENTIN SENSITIVITY
• DIRECT NEURAL STIMULATION THEORY
• FLUID OR HYDRODYNAMIC THEORY
• TRANSDUCTION THEORY
DENTINOGENESIS
• Dentin formation begins when the tooth germs has reached the
bell stage of development.

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Dentin: Structure, Composition and Functions

  • 2. Introduction • Dentin is the mineralised tissue that forms the bulk of the tooth • It is present in the crown and in the root portion of the tooth. • It is covered by enamel in the crown portion and cementum in the root portion. • It consists of mineralized collagen matrix consisting of closely packed dentinal tubules which extends the entire thickness of the dentin.
  • 3. Physical properties of dentin • Dentin is pale yellow contributes the color of the tooth • Dentin is harder than the bone and cementum but softer than the enamel. • Even though the dentin is rigid ,it is an elastic in nature ,which permits slight deformation. • The tubular structure of dentin makes it permeable ;this permeability , however decreases with increasing age.
  • 4. Chemical properties of dentin • 70% of inorganic material and 20% of organic material and 10% water • Inorganic portion –calcium hydroxy apetite crystal • Organic portion – collagen fibres mainly type I collagen. • Ground substance- non collagenous matrix protein.
  • 5. Histology of dentin 1. DENTINAL TUBULES Odontoblast forms the dentin matrix and move towards the pulp. shape of dentinal tubules
  • 6. • The thickness of dentin ranges from 3 to 10 mm. • Buccal and lingual surfaces are bigger than the mesial and distal surfaces. • The tubules are closely packed towards pulp and widely packed dentinoenamel junction. • Dentinal tubules contains terminal branches and lateral branches
  • 8. Predentin • Predentin is the newly laid ,yet to be mineralized dentin matrix. • It is the innermost layer of the dentin clos to the pulp. • Width is 6 to 10micrometer. • It becomes a part of mineralized dentin when it starts undergoing mineralization.
  • 9. Peritubular dentin • The dentin that present immediately next to the dentinal tubules is the peritubular dentin. • The minerals are deposited in the inner wall of tubule for the formation of peritubular dentin so it is called as INTRATUBULAR DENTIN. • The thickness of peritubular dentin is 0.75micrometer in outer dentin and 0.4micrometer. • The thick organic matter rich in glycosaminoglycans called lamina lamitants is seen in peri tubular dentin
  • 10. Intertubular dentin • The dentin situated between the dentinal tubules is the intertubular dentin which forms the major bulk of the dentin • It is made up of type I collagen fibres.
  • 11. Odontoblastic process • Odontoblastic process are the cytoplasmic extensions of the odontoblast which extend into the dentinal tubules • The process has a diameter of 3- 4 micrometer when it enters the dentinal tubules and tapers to 1micrometer as it extends further into the tubule.
  • 13. Primary dentin • MANTLE DENTIN Mantle dentin is a first formed dentin in the crown. It is less mineralised It is composed of larger collagen fibre It shows branching of dentinal tubules.
  • 14. Circumpulpal dentin • Circumpulpal dentin constitutes the remaining primary dentin • When compared to mantle dentin , circumpulpal dentin is more mineralised and has collagen fibres
  • 15. Interglobular dentin • During the mineralisation of dentin matrix the minerals are deposited as globules • In most areas these globules fuse to form a uniformly calcified tissue . • When some of the globules fails to fuse into a homogenous mass hypomineralised area will form, called as interglobular dentin.
  • 16. Age and functional changes • Secondary dentin • Transluscent dentin • Tertiary dentin • Sclerotic dentin • Dead tracts
  • 17. Secondary Dentin • Dentin formed after root completion • Borders the pulp • Formed by continuous , slow deposition of dentin by odontoblasts • Fewer dentinal tubules • Function : Continuous deposition leads to smaller pulp chamber and narrow root canals in old patients • Protects the pulp from exposure
  • 18. Translucent dentin • As a result of physiologic ageing, dentinal tubules become completely occluded with apatite crystals • Common in the root • Same refractive index as intertubular dentin • Occluded dentinal tubules appear translucent in ground section • Formation starts apically, proceeds cervically with age. • Function: Used in Forensic dentistry for age estimation
  • 19. Tertiary dentin • Dentin formed in response to various stimuli like attrition, caries or restorative dental procedure • Types • Reactionary dentin • Reparative dentin/Osteodentin
  • 20. Reactionary dentin • Formed by existing odontoblasts which have survived severe damage cause to them by stimulus. • Irregular appearance with fewer tubules
  • 21. Reparative dentin • Formed by newly differentiated odontoblast-like cells that replace the original odontoblasts that have been destroyed by stimulus • Newly differentiated odontoblast like cells arise from deeper regions of the pulp • Irregular with fewer , more twisted tubules • Newly differentiated odontoblast like cells get entrapped in the matrix they form to produce osteodentin.
  • 22. Possible origin of newly differentiated odontoblast like cells • Dormant odontoblast cells formed as a result of epithelial- mesenchymal interaction during tooth development • Odontoblast like cells that arise from stem cell population,without epithelial-mesenchymal interaction, under the influence of cytokines and growth factors
  • 23. Sclerotic dentin • Filling up of dentinal tubules with calcified material in response to caries, attrition, erosion • Defensive reaction • Keeps the pulp vital for a longer time • Fine meshwork of apatite crystals • Transparent • Appears light in transmitted light and dark in reflected light. • Mineral concentration is higher, crystal size is smaller • Harder
  • 24. Dead tracts • Empty dentinal tubules due to retraction of odontoblastic process or death of odontoblast. • Occurs as a result of caries, attrition, erosion • In ground sections, appear dark in transmitted light and white in reflected light • Commonly seen in narrow pulp horns • Common in older teeth
  • 25. DENTIN SENSITIVITY • DIRECT NEURAL STIMULATION THEORY • FLUID OR HYDRODYNAMIC THEORY • TRANSDUCTION THEORY
  • 26. DENTINOGENESIS • Dentin formation begins when the tooth germs has reached the bell stage of development.