Interstitium:A NEW ORGAN FOUND IN HUMAN BODY.

1. PRESENTED BY-
MURSILA ZEHRA
B.Tech (Biotechnology)
8th Semester/4th Year
1400110035
Department of Bioengineering
Integral University
Lucknow

2. The top layer of the skin
J Greogory (2016)

3. WHAT IS INTERSTITIUM?
This Organ is bodywide network of Fluid-filled
compartments, supported by a lattice of collagen fibers,
that runs throughout the human body, both within other
organs and between them.
These fluid-filled spaces were discovered in connective
tissues all over the body, including below the skin's
surface; lining the digestive tract, lungs and urinary
systems; and surrounding muscles.

4. Composition of interstitium
Collagen Types I, III, And V.
Elastin
Glycosaminoglycans, such as Hyaluronate and
Proteoglycans

5. INTERSTITIUM, AN ACCIDENTAL DISCOVERY
• Researchers already knew that there is fluid between individual cells (Interstitial fluid), but the
idea of a larger, connected interstitium in which there are fluid-filled spaces within and between
tissues was novel.
• The discovery revealed that what was previously thought to be simply dense connective tissue
sitting below the skin's surface, and surrounding our organs, is actually a complex series of
interconnected, fluid-filled compartments.
• Interstitium was Discovered through a new laser microscopic technology (pCLE)
• Probe Based Confocal laser endomicroscopy (pCLE) provides real-time histologic imaging of
human tissues at a depth of 60–70 μm during endoscopy generally after intravenous injection of
fluorescein.

6. WHEN WAS INTERSTITIUM DISCOVERED?
Interstitium as a “New” Organ in Human Body, was published on
March 27 2018 in the journal Scientific Reports.
Scientist using a special live in vivo imaging technique called a
Probe-based Confocal Laser Endomicroscopy (pCLE) discovered
the interstitium in various parts of the body including the lungs
and digestive tract.

7. HISTORY BEHIND INTERSTITIUM DISCOVERY
In 2015, Endoscopists David carr-locke and petros benias Peered
inside the body of a patient using a new technology (pCLE) that
adds a camera, laser and a tiny microscope to light up living
tissues inside a patient's bile duct found something strange they
never seen before.
It was not seen before because the fluid-filled pockets would leak
and collapse when tissues were removed and preserved in old
Microscopic techniques.
Using new technique when researchers removed and flash froze
the tissue from the bile duct of 12 more Patients, they were able to
preserve the anatomy of newly discovered structure.
Image of fresh-frozen human bile duct
(left) shows collagen bundles in blue; the
asterisks mark fluid-filled spaces of the
interstitium. Images of a fixed bile duct
from the same patient (upper and lower
right) show the collapse of spaces and
collagen bundles stickingtogether.
BENIAS ET AL., SCIENTIFIC
REPORTS, 2018.

8. Figure 1. (A,B) pCLE of bile duct after fluorescein injection shows a reticular pattern at a depth of 60–70 μm.
1.(C–E) Bile duct tissue removed at the time of Whipple surgery was frozen and ex vivo pCLE performed,
demonstrating persistence of the reticular pattern.

9. Figure A. (1) Skin stained with H&E ( 10×) (2. 40×) shows the same structures as identified in the extrahepatic bile duct.
(3). Immunostain for CD34(Brown DAB ,light blue hematozylin counterstain) highlights that the lining cells are intermittent
and often on one side of collagen bundles, but not the other.
Figure (B). Schematic showing location of identical histologic structures seen in fibroconnective tissues throughout the
body.
1 2
3

10. ADVANTAGES OF INTERSTITIUM DISCOVERY
1. Interstitium serve as shock absorber for tissues and organs.
2. this discovery could help to know the spread of cancer within the body.
3. The dermal interstitium and the fascial interstitium may be mechanistically important
in explaining edema.
4. The presence of a network of submucosal channels in the digestive and urinary tracts
could explain the greatly increased likelihood of metastasis by luminal invasive tumors
once they reach the submucosa.
5.interstitial lining cells might function as first responders in peri-tumoral sclerosis of the
pancreatico-biliary tree, tubular digestive tract, bronchial tree, urinary bladder and skin.

11. THANK YOU