Introduction, Properties, Aim & Types.

1. presentedby
mukundadnyandevkolte
msc.2nd year

3.  The stationary phase may be solid/liquid.
 In gas chromatography it is in liquid state.
 Stationary phase provide support for migration of
compound.
 It is should be rigid .
 Stationary phase can not interact with separating
compound.
 Stationary phase vary from chromatography to
chromatography.

4. Type of chromatography Material
Paper chromatography
(KK = kertas kromatografi)
Filter paper, cellulose
Thin Layer Chromatography
(KLN = Kromatografi lapisan
nipis)
Silica gel, alumina,
polyamide
Gas chromatography
(GC)
Squalene, apezion, carbowax
M
High Performance Liquid
Chromatography
(KCPT = kromatografi cecair
prestasi tinggi)
C-8, C-18, Licosorb, Silicone

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6.  The retention factor, or Rf, is defined as the distance traveled by the
compound divided by the distance traveled by the solvent
 For example, if a compound travels 2.1 cm and the solvent front
travels 2.8 cm, the Rf is 0.75:

8.  Definition :-Column chromatography is
defined as a separation process involving
uniform percolation of a liquid through a
column packed with finely divided material.
 According to stationary phase column
chromatography are different types
1. Gel permeation.
2. Ion exchange.
3. Affinity chromatography.

9.  It is utilised for determination of bio molecule and
on there molecular weight .
 It is an type of column chromatography were
stationary phase is utilised as sepharose , dextron
and sephadex etc.
 Beads have different pore size utilized as stationary
phase.
 The pore size is designed to allow the large solute
particles to pass through uninhibited.

11. •Stationary phases for ion-exchange separations are
characterized by the nature and strength of the acidic
or basic functions on their surfaces and the types of
ions that they attract and retain.
•Cation exchange is used to retain and separate
positively charged ions on a negative surface.
•Conversely, anion exchange is used to retain and
separate negatively charged ions on a positive surface.
•With each type of ion exchange, there are at least two
general approaches for separation and elution.

13. Affinity
chromatography is based
on the principle of
specific interaction
between the protein or
antigen and antibody for
separation of bio
molecules.

15. 1) . David L. Nelson , Michael M. Cox
(2005),Ch.3, amino acids and proteins
Lehninger principal of biochemistry. W. H.
Freeman and Company.

16. THANK YOU