A methyl group from S-adenosyl-methionine (SAM) is transferred to the C5 position of the pyrimidine ring of cytosine residues by DNMTs in genomic CpG dinucleotides. https://www.creative-biogene.com/Services/Drug-Discovery-Services/DNA-Methyltransferase-Screening-and-Profiling.html

2. DNA Methyltransferase Screening
Creative Biogene offers DNMTs assays, including DNMT1, DNMT3A and DNMT3B, which
will be performed by Chemiluminescent or radiolabeled method.
A methyl group from S-adenosyl-methionine (SAM) is transferred to the C5 position of
the pyrimidine ring of cytosine residues by DNMTs in genomic CpG dinucleotides. CpG
dinucleotides are preferentially located in particular regions in the genome, including
clusters in gene promoters called CpG islands. CpG methylation plays a critical role in
several aspects, inlcuding silencing retrotransposon element sequences (i.e., LINE-1 or
Alu repeats), imprinting, X chromosome inactivation, regulating gene expression, and
cellular transformation (Jason, et al. 2013).

3. Process of DNA methylation
A family of enzymes, DNA methyltransferases (DNMTs) carry out the
addition of methyl group. Five DNMT isoforms, DNMT1, DNMT2,
DNMT3a, DNMT3b, and DNMT3L are in genomic, however only three
DNMTs (DNMT1, DNMT3A, and DNMT3B) are involved in the
establishment and maintenance of DNA methylation patterns.

5. Advantages of DNMTs screening
. Inhibitors/activators screening with more than 3 assays
. IC50 determination and single point concentration assays
. Fast turnaround time
. High quality data results

6. Contact informationContact information
Provider: Creative BiogeneCreative Biogene
URL: https://www.creative-biogene.com/Services/Drug-Discovery-https://www.creative-biogene.com/Services/Drug-Discovery-
Services/DNA-Methyltransferase-Screening-and-Profiling.htmlServices/DNA-Methyltransferase-Screening-and-Profiling.html
Address: 45-1 Ramsey Road, Shirley, NY 11967, USA
Phone No. 1-631-619-7922

7. Contact informationContact information
Provider: Creative BiogeneCreative Biogene
URL: https://www.creative-biogene.com/Services/Drug-Discovery-https://www.creative-biogene.com/Services/Drug-Discovery-
Services/DNA-Methyltransferase-Screening-and-Profiling.htmlServices/DNA-Methyltransferase-Screening-and-Profiling.html
Address: 45-1 Ramsey Road, Shirley, NY 11967, USA
Phone No. 1-631-619-7922