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Mariah Hoffman Goodall, Ph.D.
8314 Beaver Court, Chestertown, MD 21620 | 540.460.3180 | mariah.goodall@gmail.com
https://www.linkedin.com/in/mariahgoodall
EDUCATION
University of Maryland School of Medicine, Baltimore, MD
Ph.D. in Molecular Medicine-3.97 GPA Aug. 2005 - Dec. 2010
Molecular Physiology and Cell Biology Track,Advisor: Dr. Terry Rogers
Area of Concentration: Molecular Medicine - Cardiovascular Physiology
Virginia Military Institute, Lexington, VA
B.S. Distinguished Graduate in Biology- 3.82 GPA Aug. 2000 - May 2004
Areas of Concentration: Cell and Molecular Core,Organismal Biology Core
Minor: Chemistry
RESEARCH EXPERIENCE
Washington College, Department of Biology, Chestertown, MD
Independent research projects with undergraduate students in my biology labs Jan. 2014 – May 2015
Structural and functional characterization of striated muscles within and across species
at the molecular, cellular, tissue, and whole organism level.
University of Maryland School of Medicine, Department of Orthopaedics, Baltimore, MD
Postdoctoral Fellow Jan. 2011 – Sept. 2012
Structural and functional evaluation of branched myofibers in old mice lacking
keratin19 and/or desmin intermediate filaments.
University of Maryland School of Medicine, Baltimore, MD
Graduate Research Assistant Sept. 2005 – Dec.2010
Thesis Research: “Novel Function of Cardiac Protein Kinase D as a Dynamic
Regulator of Ca2+
Sensitivity of Contraction”
Virginia Military Institute, Lexington, VA
Undergraduate Research Assistant, Independent Study June 2001 – May 2004
Live cell imaging of Ca2+
during the chemoattractant off-response in Paramecium
tetraurelia.
HONORS AND AWARDS
University of Maryland School of Medicine
 NIH T32 Interdisciplinary Training Program in Muscle Biology postdoctoral trainee Aug. 2011 – Aug. 2012
 31st
International Society for Heart Research conference poster competition winner May 2009
 NIH T32 Interdisciplinary Training Program in Muscle Biology predoctoral trainee June 2008 – June 2009
 NIH T32 Integrative Membrane Biology predoctoral trainee May 2006 – May 2008
 Molecular Medicine Program Outstanding Student Award August 2007
Virginia Military Institute
 Virginia Military Institute Academic Scholar, VMI August 2000 – May 2004
 Alfred H. Knowles Award for significant accomplishments in biology, VMI May 2004
 Beta Beta Beta Biological Honor Society, VMI April 2004
 Iota Sigma Pi National Honor Society for Women in Chemistry, VMI November 2002
 Paul R. Meyer ’24 Award for significant accomplishments in biology, VMI May 2001
 Phi Eta Sigma National Freshman Honor Society, VMI April 2001
Mariah H. Goodall, Ph.D. Page 2
PUBLICATIONS
 Goodall, M.H., Ward, C.W., Pratt, S.J., Bloch, R.J., and Lovering, R.M. Structural and functional
evaluation of branched myofibers lacking intermediate filaments. Am J Physiol Cell Physiol. 2012;
303 (2): C224-32.
 Lovering, R.M., Roche, J.A., Goodall, M.H., Clark, B.B., McMillan, A. An in vivo Rodent Model of
Contraction-induced Injury and Non-invasive Monitoring of Recovery. J. Vis. Exp. 2011; (51).
 Goodall, M.H., Wardlow, R.D., Goldblum, R.R., Ziman, A., Lederer, W.J., Randall, W., and Rogers,
T.B. Novel function of cardiac protein kinase D1 as a dynamic regulator of Ca2 +
sensitivity of
contraction. J. Biol. Chem. 2010; 285 (53): 41686-41700.
 Zhou, X.W., Mudannayak, M., Gre e n, M., Gigena, M.S., Wang, G., Shen, R.F., and Rogers, T.B.
Proteomic studies of PP2A-B56gamma1 phosphatase complexes reveal phosphorylation-regulated
partners in cardiac local signaling. J. Proteome Res. 2007; 6 (9): 3433-3442.
TEACHING EXPERIENCE
Washington College, Chestertown,MD
Lecturer in Biology Adjunct Professor
 Muscle physiology course development, lecture and lab instruction Jan. 2014 – May 2015
Taught a 300-level muscle biology course, including lecture and lab, to sophomore, junior
and senior science majors. Responsible for entire course and lab development, equipment
purchasing for new course, coordinating equipment use within biology department, all lab
setup/takedown, course assignments, grading, course setup on Canvas.
 Exercise physiology course development, lecture and lab instruction Aug. 2012 - May 2015
Taught a 100-level current topics exercise physiology course, including lecture and lab, to
non-majors that need a science distribution credit. Responsible for entire course and lab
development, equipment purchasing for new course,coordinating equipment use with
multiple departments on campus, all lab setup/takedown, course assignments, grading,
course setup on Canvas. Supervised severalteaching assistants to help with lab instruction.
 General biology lab instructor Aug. 2011 - Dec.2014
Taught 100-level spring and fall sections of generalbiology lab to science majors.
Responsible for brief prelab on specific lab concepts/objectives and instruction/tips on
smooth running of lab, basics on data analysis and how best to graphically present class data
using excel and/or LoggerPro computer software,emphasis on scientific writing basics that
results in a well-written lab report of a study. Supervised severalteaching assistants to help
with lab instruction, lab prep, setup and takedown.
 General biology lab prep, setup, and takedown Aug. 2011 - May 2015
Responsible for general biology lab prep, setup and takedown for approximately 150 – 200
students per semester (10+
lab sections) to optimize flow, transition, and collection of data.
Organized a list of materials required for each lab, as well as created a visual diagram of the
lab setup for each lab run throughout the semester for different adjunct lab instructors to
reference during lab instruction.
University of Maryland School of Medicine, Baltimore, MD
Student/Lab Technician Mentoring
 Stephen Pratt,Lab Technician working in postdoctoral lab Jan. 2011 - Aug. 2012
Mariah H. Goodall, Ph.D. Page 3
Taught general laboratory maintenance, isolation of skeletal muscles and individual muscle
fibers, how to make solutions, cell culture techniques, calcium loading, imaging and
manganese quench technique, morphology assessment,paraformaldehyde fixation, confocal
microscopy training, general feedback on literature searches,data analysis, poster
presentations, and manuscript writing.
 Robert Wardlow, Undergraduate Student working in predoctoral lab 2009 - 2010
Taught general laboratory maintenance, isolation, culturing and adenoviral infection of
cardiomyocytes, cell harvesting and Western blotting, field stimulation of cardiomyocytes
and simultaneous functional/physiological measurements of sarcomere length (contractility)
and [Ca2+
]i transients, data analysis and presentation of data, and critiqued poster and
platform presentation skills.
 Rebecca Goldblum, High School Senior working in predoctoral lab 2007 - 2008
Taught general laboratory maintenance, field stimulation of cardiomyocytes and
simultaneous functional/physiological measurements of sarcomere length (contractility) and
[Ca2+
]i transients, data analysis and presentation of data, and critiqued poster presentation
skills.
John Randolph Tucker High School, Richmond, VA
Henrico County Science Teacher – Honors & College Prep Chemistry; Biology Aug. 2004 - June 2005
 Developed syllabus and overall course structure, including lab practicum, according to
Virginia Standards of Learning, and administered all grades.
 Assistant Coach – cross-country
 Assistant Coach – indoor track
 Assistant Coach – outdoor track and field
Virginia Military Institute, Lexington, VA
Cell Biology Lab Assistant Aug. 2002 - Dec.2002
 Assisted in the teaching of a cell biology lab section; general laboratory procedures
and maintenance.
ABSTRACT PUBLICATIONS
 Goodall, M.H., Goldblum, R.R., Ziman, A., Lederer, W.J., Randall, W., and Rogers, T.B. Novel
Functions of Protein Kinase D in Cardiac Excitation-Contraction Coupling. J. Mol. Cell Cardiol.
2009; 46 (5): S29.
 Goodall, M.H., Teos, L.Y., Goldblum, R.R., Ziman, A., duBell W.H., Lederer, W.J., Randall, W., and
Rogers, T.B. Novel Functions of Protein Kinase D in Cardiac Excitation-Contraction Coupling.
Biophysical Journal. 2009; 96 (3): 622a-623a.
 M.H. Green, H.J. Brown and W.E. Bell. The ryanodine receptor antagonist dantrolene alters
swimming behavior and causes mortality in Paramecium tetraurelia. Chemical Senses. 2005; 30 (3):
268.
 W.E. Bell, M.H. Green and J. Van Houten. Live cell imaging of Ca2 +
during the chemoattractant off-
response in Paramecium. Chemical Senses. 2003; 28 (6): 555.
 W.E. Bell and M.H. Green. Live cell imaging of Mg2 +
influx during the biotin off-response in
Paramecium. Chemical Senses. 2002; 27 (7): 662.
Mariah H. Goodall, Ph.D. Page 4
PRESENTATION EXPERIENCE
 Structural and functional evaluation of branched myofibers lacking intermediate filaments. Muscle
Biology Training Program Mini-Retreat, Poster Presentation, University of Maryland, April 2012.
Mariah H. Goodall, Christopher W. Ward, Stephen J.P. Pratt,Robert J. Bloch, and Richard M. Lovering.
 Structural and functional evaluation of branched myofibers in old mice lacking keratin 19 and/or desmin
intermediate filaments. Muscle Biology Training Program Mini-Retreat, Poster Presentation,University
of Maryland, April 15, 2011. Mariah H. Goodall, Brett B. Clark, Stephen J.P. Pratt,Andrea O'Neill,
Christopher W. Ward, and Richard M. Lovering.
 Novel Functions of Protein Kinase D in Cardiac Excitation-Contraction Coupling. ISHR North American
Section Meeting, Baltimore, MD, May 28, 2009. Mariah H. Goodall, Rebecca R. Goldblum, Andrew
Ziman, W. Jonathan Lederer,William Randall and Terry B. Rogers.
 Novel Functions of Protein Kinase D in Cardiac Excitation-Contraction Coupling. 11th
AnnualProgram
in Membrane Biology Retreat,University of Maryland, May 21, 2009. Mariah H. Goodall, Rebecca R.
Goldblum, Andrew Ziman, W. Jonathan Lederer,William Randall and Terry B. Rogers.
 Novel Functions of Protein Kinase D in Cardiac Excitation-Contraction Coupling. Muscle Biology
Training Program Mini-Retreat, Poster Presentation,University of Maryland, April 17, 2009. Mariah H.
Goodall, Rebecca R. Goldblum, Andrew Ziman, W. Jonathan Lederer, William Randall and Terry B.
Rogers.
 Novel Functions of Protein Kinase D in Cardiac Excitation-Contraction Coupling. 53rd
Annual
Biophysical Society Meeting, Poster Presentation,Boston, MA, March 4, 2009. Mariah H. Goodall,
Leyla Y. Teos, Rebecca R. Goldblum, Andrew Ziman, William H. duBell, W. Jonathan Lederer,William
Randall and Terry B. Rogers.
 Protein Kinase D Activates L-Type Ca2+
Channel Current in Ventricular Myocytes. 3rd
Annual National
Graduate Student Research Festival, Poster Presentation, National Institutes of Health, Bethesda, MD,
September 11, 2008. Mariah H. Green,Leyla Teos, W. Jonathan Lederer,William Randall and Terry B.
Rogers
 Protein Kinase D Activates L-Type Ca2+
Channel Current in Ventricular Myocytes. Muscle Biology
Training Program Mini-Retreat, Poster Presentation,University of Maryland, May 2, 2008. Mariah H.
Green,Leyla Teos,W. Jonathan Lederer,William Randall and Terry B. Rogers.
 The Ryanodine Receptor Antagonist Dantrolene Alters Swimming Behavior and Causes Mortality in
Parameciumtetraurelia. Association of Chemoreception Sciences 26th
Annual Meeting, Sarasota,FL,
2004. M.H. Green,H.J. Brown and W.E. Bell.
 The Ryanodine Receptor Antagonist Dantrolene Alters Swimming Behavior and Causes Mortality in
Parameciumtetraurelia. American Society for Microbiology, Virginia Branch Meeting, 2003. M.H.
Green,H.J. Brown and W.E. Bell.
 Live Cell Imaging of Ca2+
During the Chemoattractant Off-Response in Paramecium. Association of
Chemoreception Sciences 25th
Annual Meeting, Sarasota,FL, 2003. W.E. Bell, M.H. Green and J. Van
Houten.
 Live Cell Imaging of Mg2+
During the Biotin Off-Response in Paramecium. Association of
Chemoreception Sciences 24th
Annual Meeting, Sarasota,FL, 2002. W.E. Bell and M.H. Green.
 Live Cell Imaging of CalciumDuring the Chemoattractant Off-Response in Paramecium. American
Society for Microbiology, Virginia Branch Meeting, 2002. W.E. Bell, M.H. Green and J. Van Houten.
Mariah H. Goodall, Ph.D. Page 5
 Live Cell Imaging of CalciumDuring the Chemoattractant Off-Response in Paramecium. Mid-Atlantic
Regional Conference of Undergraduate Scholarship, 2002. W.E. Bell, M.H. Green and J. Van Houten.
 Mortality of Parameciumduring Epi-fluorescent Microscopy: Effects of Different Excitation Wavelengths
and Membrane Permeable Dyes. American Society for Microbiology, Virginia Branch Meeting, 2001.
M.H. Green and W.E. Bell.
AFFILIATIONS AND PROFESSIONAL MEMBERSHIPS
Dragonfly Heart Camp Board of Directors May 2014 - Present
USA Triathlon Feb. 2012 – Feb. 2014
American College of Sports Medicine 2012
Association for Women in Science 2012
Cardiac Muscle Society 2009-2010
Biophysical Society 2009-2010
Association of Chemoreception Sciences 2003 - 2004
American Society of Microbiology, Virginia Branch 2001 – 2003
LABORATORY TECHNIQUES AND INTERESTS
Applied Physiology & Exercise Physiology Techniques:
 Determination of maximum heart rate, calculation of heart rate training zones, and heart rate training using Ekho
and Polar heart rate monitors
 Musculoskeletal fitness assessments of muscular strength using 1-repetition max bench- and leg-press and handgrip
strength using Smedley and Vernier hand dynamometers as well as upper body and trunk muscular endurance
 Blood pressure assessment sitting at rest,standing, laying down, during isometric muscle contractions, and blood
pressure recovery following low, moderate, and high intensity exercise
 Pulmonary function testing using Vernier spirometers to measure tidal volume, forced vital capacity (FVC),forced
expiratory volume (FEV1.0),peak expiratory flow (PEF) and calculate the FEV1.0/FVC ratio
 Anaerobic fitness field test assessments of horizontal power, vertical power of countermovement and static vertical
jumps using a Vertec and a switch mat, and the Margaria Kalamen power stair climb test
 Aerobic fitness field test assessments to estimate VO2max using the Queens College Step test in combination with the
Cooper 1.5 mile run/walk test or Rockport Fitness walking test
 Body composition testing to calculate BMI, waist to hip ratio, and estimate body fat percentage using skinfold
calipers to measure subcutaneous fat thickness at seven anatomical sites vs. body fat percentage obtained from
bioelectrical impedance
Cell isolation and cell culturing:
 CITI certifications in working with mice and rats in research settings
 Isolation of adult rat ventricular myocytes (ARVMs) after Langendorff perfusion and standard enzymatic digestion
 ARVM cell culturing for 24 - 48 hr
 Isolation of adult mouse flexor digitorum brevis (FDB) skeletal muscles
Molecular Techniques:
 Adenoviral infection of ARVMs to overexpress a dominant negative or constitutively active form of protein kinase
D to mimic conditions where this enzyme is underactive or overactive (i.e. hypertrophy/heart failure)
Physiological & Molecular Physiology Techniques:
 Field stimulation of cultured, intact ARVMs and skeletal muscle flexor digitorum brevis fibers
 Simultaneous functional measurements of sarcomere length (contractility) and [Ca2+
]i transients using IonOptix
video based myocyte sarcomere spacing, SarcLen,and fluorescence acquisition system
Mariah H. Goodall, Ph.D. Page 6
 Real-time imaging of Ca2+
sparks and analysis in ARVMs
 Morphological comparison and identification of malformed myofibers in isolated FDB muscles from adult mouse
 Examination of excitation-contraction function in FDBs via assessment of global, electrically evoked Ca2+
signals
using the ratiometric Ca2+
indicator Indo1PE-AM
 Manganese quench of Indo-1 PE Ca2+
-indicator dye to assay the sarcolemmal permeability of single, enzymatically-
isolated FDB adult skeletal myofibers to monovalent cations (i.e. Ca2+
)
Immunofluorescence and Microscopy:
 Light microscopy and epifluorescent/laser confocal microscopy
 Immunocytochemistry, double-labeling and confocal microscopy of fixed ARVMs
 Double-label confocal fluorescent microscopy of fixed ARVMs after field stimulation
 Real-time confocal imagining of Plum-tagged constructs in live, cultured ARVMs
 Immunocytochemistry, double/triple labeling, and confocal microscopy of fixed FDB muscles
 Ca2+
imaging with several Ca2+
-indicator dyes, Ca2+
loading techniques in various cell lines/cultures, fluorescent
Ca2+
imaging using various cell lines/cultures, and novel manganese quench technique to assess sarcolemmal
permeability to monovalent cations
Biochemical Techniques:
 Cell harvesting of cultured whole-cell ARVM extracts and BioRad protein assay using spectrophotometry
 Western blotting, stripping and re-probing
 Optimization of Western blot protocol for various antibodies
 Cell harvesting after field stimulation
Computer Skills and Data Analysis:
 Imaging and scanning by using Photoshop and NIH ImageJ software
 Origin and SigmaPlot graphing programs
 Corel Draw graphics program
 Microsoft Word, Microsoft Excel, and Power Point, Microsoft Office
 SigmaStat and GB Stat for statistical comparisons
 Densitometric analysis of Westerns using NIH ImageJ software
 Creating phase-plane loop plots of contractile shortening as a function of the Ca2+
transient to demonstrate dynamic
Ca2+
sensitivity
 Two-dimensional analysis of confocal images using NIH ImageJ software
 Creating plot profiles of relative pixel intensity using NIH ImageJ software
 3-D reconstruction analysis of confocal images using Velocity software
 Vernier sensors/ products (dissolved oxygen sensor, oxygen sensor, handgrip dynamometer, spectrophotometer,
etc.) and LoggerPro software for data collection/analysis
 UV transilluminator with Vernier software

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Mariah H Goodall CV

  • 1. Mariah Hoffman Goodall, Ph.D. 8314 Beaver Court, Chestertown, MD 21620 | 540.460.3180 | mariah.goodall@gmail.com https://www.linkedin.com/in/mariahgoodall EDUCATION University of Maryland School of Medicine, Baltimore, MD Ph.D. in Molecular Medicine-3.97 GPA Aug. 2005 - Dec. 2010 Molecular Physiology and Cell Biology Track,Advisor: Dr. Terry Rogers Area of Concentration: Molecular Medicine - Cardiovascular Physiology Virginia Military Institute, Lexington, VA B.S. Distinguished Graduate in Biology- 3.82 GPA Aug. 2000 - May 2004 Areas of Concentration: Cell and Molecular Core,Organismal Biology Core Minor: Chemistry RESEARCH EXPERIENCE Washington College, Department of Biology, Chestertown, MD Independent research projects with undergraduate students in my biology labs Jan. 2014 – May 2015 Structural and functional characterization of striated muscles within and across species at the molecular, cellular, tissue, and whole organism level. University of Maryland School of Medicine, Department of Orthopaedics, Baltimore, MD Postdoctoral Fellow Jan. 2011 – Sept. 2012 Structural and functional evaluation of branched myofibers in old mice lacking keratin19 and/or desmin intermediate filaments. University of Maryland School of Medicine, Baltimore, MD Graduate Research Assistant Sept. 2005 – Dec.2010 Thesis Research: “Novel Function of Cardiac Protein Kinase D as a Dynamic Regulator of Ca2+ Sensitivity of Contraction” Virginia Military Institute, Lexington, VA Undergraduate Research Assistant, Independent Study June 2001 – May 2004 Live cell imaging of Ca2+ during the chemoattractant off-response in Paramecium tetraurelia. HONORS AND AWARDS University of Maryland School of Medicine  NIH T32 Interdisciplinary Training Program in Muscle Biology postdoctoral trainee Aug. 2011 – Aug. 2012  31st International Society for Heart Research conference poster competition winner May 2009  NIH T32 Interdisciplinary Training Program in Muscle Biology predoctoral trainee June 2008 – June 2009  NIH T32 Integrative Membrane Biology predoctoral trainee May 2006 – May 2008  Molecular Medicine Program Outstanding Student Award August 2007 Virginia Military Institute  Virginia Military Institute Academic Scholar, VMI August 2000 – May 2004  Alfred H. Knowles Award for significant accomplishments in biology, VMI May 2004  Beta Beta Beta Biological Honor Society, VMI April 2004  Iota Sigma Pi National Honor Society for Women in Chemistry, VMI November 2002  Paul R. Meyer ’24 Award for significant accomplishments in biology, VMI May 2001  Phi Eta Sigma National Freshman Honor Society, VMI April 2001
  • 2. Mariah H. Goodall, Ph.D. Page 2 PUBLICATIONS  Goodall, M.H., Ward, C.W., Pratt, S.J., Bloch, R.J., and Lovering, R.M. Structural and functional evaluation of branched myofibers lacking intermediate filaments. Am J Physiol Cell Physiol. 2012; 303 (2): C224-32.  Lovering, R.M., Roche, J.A., Goodall, M.H., Clark, B.B., McMillan, A. An in vivo Rodent Model of Contraction-induced Injury and Non-invasive Monitoring of Recovery. J. Vis. Exp. 2011; (51).  Goodall, M.H., Wardlow, R.D., Goldblum, R.R., Ziman, A., Lederer, W.J., Randall, W., and Rogers, T.B. Novel function of cardiac protein kinase D1 as a dynamic regulator of Ca2 + sensitivity of contraction. J. Biol. Chem. 2010; 285 (53): 41686-41700.  Zhou, X.W., Mudannayak, M., Gre e n, M., Gigena, M.S., Wang, G., Shen, R.F., and Rogers, T.B. Proteomic studies of PP2A-B56gamma1 phosphatase complexes reveal phosphorylation-regulated partners in cardiac local signaling. J. Proteome Res. 2007; 6 (9): 3433-3442. TEACHING EXPERIENCE Washington College, Chestertown,MD Lecturer in Biology Adjunct Professor  Muscle physiology course development, lecture and lab instruction Jan. 2014 – May 2015 Taught a 300-level muscle biology course, including lecture and lab, to sophomore, junior and senior science majors. Responsible for entire course and lab development, equipment purchasing for new course, coordinating equipment use within biology department, all lab setup/takedown, course assignments, grading, course setup on Canvas.  Exercise physiology course development, lecture and lab instruction Aug. 2012 - May 2015 Taught a 100-level current topics exercise physiology course, including lecture and lab, to non-majors that need a science distribution credit. Responsible for entire course and lab development, equipment purchasing for new course,coordinating equipment use with multiple departments on campus, all lab setup/takedown, course assignments, grading, course setup on Canvas. Supervised severalteaching assistants to help with lab instruction.  General biology lab instructor Aug. 2011 - Dec.2014 Taught 100-level spring and fall sections of generalbiology lab to science majors. Responsible for brief prelab on specific lab concepts/objectives and instruction/tips on smooth running of lab, basics on data analysis and how best to graphically present class data using excel and/or LoggerPro computer software,emphasis on scientific writing basics that results in a well-written lab report of a study. Supervised severalteaching assistants to help with lab instruction, lab prep, setup and takedown.  General biology lab prep, setup, and takedown Aug. 2011 - May 2015 Responsible for general biology lab prep, setup and takedown for approximately 150 – 200 students per semester (10+ lab sections) to optimize flow, transition, and collection of data. Organized a list of materials required for each lab, as well as created a visual diagram of the lab setup for each lab run throughout the semester for different adjunct lab instructors to reference during lab instruction. University of Maryland School of Medicine, Baltimore, MD Student/Lab Technician Mentoring  Stephen Pratt,Lab Technician working in postdoctoral lab Jan. 2011 - Aug. 2012
  • 3. Mariah H. Goodall, Ph.D. Page 3 Taught general laboratory maintenance, isolation of skeletal muscles and individual muscle fibers, how to make solutions, cell culture techniques, calcium loading, imaging and manganese quench technique, morphology assessment,paraformaldehyde fixation, confocal microscopy training, general feedback on literature searches,data analysis, poster presentations, and manuscript writing.  Robert Wardlow, Undergraduate Student working in predoctoral lab 2009 - 2010 Taught general laboratory maintenance, isolation, culturing and adenoviral infection of cardiomyocytes, cell harvesting and Western blotting, field stimulation of cardiomyocytes and simultaneous functional/physiological measurements of sarcomere length (contractility) and [Ca2+ ]i transients, data analysis and presentation of data, and critiqued poster and platform presentation skills.  Rebecca Goldblum, High School Senior working in predoctoral lab 2007 - 2008 Taught general laboratory maintenance, field stimulation of cardiomyocytes and simultaneous functional/physiological measurements of sarcomere length (contractility) and [Ca2+ ]i transients, data analysis and presentation of data, and critiqued poster presentation skills. John Randolph Tucker High School, Richmond, VA Henrico County Science Teacher – Honors & College Prep Chemistry; Biology Aug. 2004 - June 2005  Developed syllabus and overall course structure, including lab practicum, according to Virginia Standards of Learning, and administered all grades.  Assistant Coach – cross-country  Assistant Coach – indoor track  Assistant Coach – outdoor track and field Virginia Military Institute, Lexington, VA Cell Biology Lab Assistant Aug. 2002 - Dec.2002  Assisted in the teaching of a cell biology lab section; general laboratory procedures and maintenance. ABSTRACT PUBLICATIONS  Goodall, M.H., Goldblum, R.R., Ziman, A., Lederer, W.J., Randall, W., and Rogers, T.B. Novel Functions of Protein Kinase D in Cardiac Excitation-Contraction Coupling. J. Mol. Cell Cardiol. 2009; 46 (5): S29.  Goodall, M.H., Teos, L.Y., Goldblum, R.R., Ziman, A., duBell W.H., Lederer, W.J., Randall, W., and Rogers, T.B. Novel Functions of Protein Kinase D in Cardiac Excitation-Contraction Coupling. Biophysical Journal. 2009; 96 (3): 622a-623a.  M.H. Green, H.J. Brown and W.E. Bell. The ryanodine receptor antagonist dantrolene alters swimming behavior and causes mortality in Paramecium tetraurelia. Chemical Senses. 2005; 30 (3): 268.  W.E. Bell, M.H. Green and J. Van Houten. Live cell imaging of Ca2 + during the chemoattractant off- response in Paramecium. Chemical Senses. 2003; 28 (6): 555.  W.E. Bell and M.H. Green. Live cell imaging of Mg2 + influx during the biotin off-response in Paramecium. Chemical Senses. 2002; 27 (7): 662.
  • 4. Mariah H. Goodall, Ph.D. Page 4 PRESENTATION EXPERIENCE  Structural and functional evaluation of branched myofibers lacking intermediate filaments. Muscle Biology Training Program Mini-Retreat, Poster Presentation, University of Maryland, April 2012. Mariah H. Goodall, Christopher W. Ward, Stephen J.P. Pratt,Robert J. Bloch, and Richard M. Lovering.  Structural and functional evaluation of branched myofibers in old mice lacking keratin 19 and/or desmin intermediate filaments. Muscle Biology Training Program Mini-Retreat, Poster Presentation,University of Maryland, April 15, 2011. Mariah H. Goodall, Brett B. Clark, Stephen J.P. Pratt,Andrea O'Neill, Christopher W. Ward, and Richard M. Lovering.  Novel Functions of Protein Kinase D in Cardiac Excitation-Contraction Coupling. ISHR North American Section Meeting, Baltimore, MD, May 28, 2009. Mariah H. Goodall, Rebecca R. Goldblum, Andrew Ziman, W. Jonathan Lederer,William Randall and Terry B. Rogers.  Novel Functions of Protein Kinase D in Cardiac Excitation-Contraction Coupling. 11th AnnualProgram in Membrane Biology Retreat,University of Maryland, May 21, 2009. Mariah H. Goodall, Rebecca R. Goldblum, Andrew Ziman, W. Jonathan Lederer,William Randall and Terry B. Rogers.  Novel Functions of Protein Kinase D in Cardiac Excitation-Contraction Coupling. Muscle Biology Training Program Mini-Retreat, Poster Presentation,University of Maryland, April 17, 2009. Mariah H. Goodall, Rebecca R. Goldblum, Andrew Ziman, W. Jonathan Lederer, William Randall and Terry B. Rogers.  Novel Functions of Protein Kinase D in Cardiac Excitation-Contraction Coupling. 53rd Annual Biophysical Society Meeting, Poster Presentation,Boston, MA, March 4, 2009. Mariah H. Goodall, Leyla Y. Teos, Rebecca R. Goldblum, Andrew Ziman, William H. duBell, W. Jonathan Lederer,William Randall and Terry B. Rogers.  Protein Kinase D Activates L-Type Ca2+ Channel Current in Ventricular Myocytes. 3rd Annual National Graduate Student Research Festival, Poster Presentation, National Institutes of Health, Bethesda, MD, September 11, 2008. Mariah H. Green,Leyla Teos, W. Jonathan Lederer,William Randall and Terry B. Rogers  Protein Kinase D Activates L-Type Ca2+ Channel Current in Ventricular Myocytes. Muscle Biology Training Program Mini-Retreat, Poster Presentation,University of Maryland, May 2, 2008. Mariah H. Green,Leyla Teos,W. Jonathan Lederer,William Randall and Terry B. Rogers.  The Ryanodine Receptor Antagonist Dantrolene Alters Swimming Behavior and Causes Mortality in Parameciumtetraurelia. Association of Chemoreception Sciences 26th Annual Meeting, Sarasota,FL, 2004. M.H. Green,H.J. Brown and W.E. Bell.  The Ryanodine Receptor Antagonist Dantrolene Alters Swimming Behavior and Causes Mortality in Parameciumtetraurelia. American Society for Microbiology, Virginia Branch Meeting, 2003. M.H. Green,H.J. Brown and W.E. Bell.  Live Cell Imaging of Ca2+ During the Chemoattractant Off-Response in Paramecium. Association of Chemoreception Sciences 25th Annual Meeting, Sarasota,FL, 2003. W.E. Bell, M.H. Green and J. Van Houten.  Live Cell Imaging of Mg2+ During the Biotin Off-Response in Paramecium. Association of Chemoreception Sciences 24th Annual Meeting, Sarasota,FL, 2002. W.E. Bell and M.H. Green.  Live Cell Imaging of CalciumDuring the Chemoattractant Off-Response in Paramecium. American Society for Microbiology, Virginia Branch Meeting, 2002. W.E. Bell, M.H. Green and J. Van Houten.
  • 5. Mariah H. Goodall, Ph.D. Page 5  Live Cell Imaging of CalciumDuring the Chemoattractant Off-Response in Paramecium. Mid-Atlantic Regional Conference of Undergraduate Scholarship, 2002. W.E. Bell, M.H. Green and J. Van Houten.  Mortality of Parameciumduring Epi-fluorescent Microscopy: Effects of Different Excitation Wavelengths and Membrane Permeable Dyes. American Society for Microbiology, Virginia Branch Meeting, 2001. M.H. Green and W.E. Bell. AFFILIATIONS AND PROFESSIONAL MEMBERSHIPS Dragonfly Heart Camp Board of Directors May 2014 - Present USA Triathlon Feb. 2012 – Feb. 2014 American College of Sports Medicine 2012 Association for Women in Science 2012 Cardiac Muscle Society 2009-2010 Biophysical Society 2009-2010 Association of Chemoreception Sciences 2003 - 2004 American Society of Microbiology, Virginia Branch 2001 – 2003 LABORATORY TECHNIQUES AND INTERESTS Applied Physiology & Exercise Physiology Techniques:  Determination of maximum heart rate, calculation of heart rate training zones, and heart rate training using Ekho and Polar heart rate monitors  Musculoskeletal fitness assessments of muscular strength using 1-repetition max bench- and leg-press and handgrip strength using Smedley and Vernier hand dynamometers as well as upper body and trunk muscular endurance  Blood pressure assessment sitting at rest,standing, laying down, during isometric muscle contractions, and blood pressure recovery following low, moderate, and high intensity exercise  Pulmonary function testing using Vernier spirometers to measure tidal volume, forced vital capacity (FVC),forced expiratory volume (FEV1.0),peak expiratory flow (PEF) and calculate the FEV1.0/FVC ratio  Anaerobic fitness field test assessments of horizontal power, vertical power of countermovement and static vertical jumps using a Vertec and a switch mat, and the Margaria Kalamen power stair climb test  Aerobic fitness field test assessments to estimate VO2max using the Queens College Step test in combination with the Cooper 1.5 mile run/walk test or Rockport Fitness walking test  Body composition testing to calculate BMI, waist to hip ratio, and estimate body fat percentage using skinfold calipers to measure subcutaneous fat thickness at seven anatomical sites vs. body fat percentage obtained from bioelectrical impedance Cell isolation and cell culturing:  CITI certifications in working with mice and rats in research settings  Isolation of adult rat ventricular myocytes (ARVMs) after Langendorff perfusion and standard enzymatic digestion  ARVM cell culturing for 24 - 48 hr  Isolation of adult mouse flexor digitorum brevis (FDB) skeletal muscles Molecular Techniques:  Adenoviral infection of ARVMs to overexpress a dominant negative or constitutively active form of protein kinase D to mimic conditions where this enzyme is underactive or overactive (i.e. hypertrophy/heart failure) Physiological & Molecular Physiology Techniques:  Field stimulation of cultured, intact ARVMs and skeletal muscle flexor digitorum brevis fibers  Simultaneous functional measurements of sarcomere length (contractility) and [Ca2+ ]i transients using IonOptix video based myocyte sarcomere spacing, SarcLen,and fluorescence acquisition system
  • 6. Mariah H. Goodall, Ph.D. Page 6  Real-time imaging of Ca2+ sparks and analysis in ARVMs  Morphological comparison and identification of malformed myofibers in isolated FDB muscles from adult mouse  Examination of excitation-contraction function in FDBs via assessment of global, electrically evoked Ca2+ signals using the ratiometric Ca2+ indicator Indo1PE-AM  Manganese quench of Indo-1 PE Ca2+ -indicator dye to assay the sarcolemmal permeability of single, enzymatically- isolated FDB adult skeletal myofibers to monovalent cations (i.e. Ca2+ ) Immunofluorescence and Microscopy:  Light microscopy and epifluorescent/laser confocal microscopy  Immunocytochemistry, double-labeling and confocal microscopy of fixed ARVMs  Double-label confocal fluorescent microscopy of fixed ARVMs after field stimulation  Real-time confocal imagining of Plum-tagged constructs in live, cultured ARVMs  Immunocytochemistry, double/triple labeling, and confocal microscopy of fixed FDB muscles  Ca2+ imaging with several Ca2+ -indicator dyes, Ca2+ loading techniques in various cell lines/cultures, fluorescent Ca2+ imaging using various cell lines/cultures, and novel manganese quench technique to assess sarcolemmal permeability to monovalent cations Biochemical Techniques:  Cell harvesting of cultured whole-cell ARVM extracts and BioRad protein assay using spectrophotometry  Western blotting, stripping and re-probing  Optimization of Western blot protocol for various antibodies  Cell harvesting after field stimulation Computer Skills and Data Analysis:  Imaging and scanning by using Photoshop and NIH ImageJ software  Origin and SigmaPlot graphing programs  Corel Draw graphics program  Microsoft Word, Microsoft Excel, and Power Point, Microsoft Office  SigmaStat and GB Stat for statistical comparisons  Densitometric analysis of Westerns using NIH ImageJ software  Creating phase-plane loop plots of contractile shortening as a function of the Ca2+ transient to demonstrate dynamic Ca2+ sensitivity  Two-dimensional analysis of confocal images using NIH ImageJ software  Creating plot profiles of relative pixel intensity using NIH ImageJ software  3-D reconstruction analysis of confocal images using Velocity software  Vernier sensors/ products (dissolved oxygen sensor, oxygen sensor, handgrip dynamometer, spectrophotometer, etc.) and LoggerPro software for data collection/analysis  UV transilluminator with Vernier software