2. TISSUE CULTURE IN INDIA
• First commercial tissue culture in India in 1987.
– A.V.Thomas & Company –Kerala (Cochin).
• In 1988 – Indo American Hybrid Seeds at Banglore.
– (10 million plants/annum)
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14. TYPES OF SUSPENSION CULTURE
1.BATCH SUSPENSION CULTURE
2.SEMI-CONTINOUS CULTURE
3.CONTINOUS CULTURE
• Open
• Closed
26. HARDENING
• Healthy plantlets are exposed to sunlight in a stepwise
manner.
• It is a gradual acclimatization of in vitro growth to in vivo
condition.
• The plantlets ae transferred to the pots/polythene bags .
• Plants are kept in Hardening room where controlled
conditions of light ,humidity & temperature are
maintained.
27. ADVANTAGES
• Production of improved crop varieties
• Production of disease free plants
• Genetic transformation
• Production of secondary metabolites.
• Production of varieties tolerant to
– Salinity
– Drought
– Heat stress
28. TISSUE CULTURE FIRMS IN T.N
• Grow More Biotech
• Shankar Biotech
• Greenwell Biotech
• KF Bio Plants
• Kala Biotech
29. The farmers get a net income of more than 70,000 per acre
annually.
THE HINDU