published

1. Evaluation of Anti-Anxiety activity of Rhizome of
Cyperus rotundas-*
PRESENTED BY PRIYA PATEL
UID - 20MPH2019
SUPERVISOR-Asst.Professor RITIKA SHARMA

2. CONTENT
• INTRODUCTION
• TYPES OF ANXIETY
• PATHOPHYSIOLOGY
• INDUCED MODEL
• AIM AND OBJECTIVE
• PLAN AND WORK
• REFERENCE

3. INTRODUCTION
• It is an emotional state, unpleasant in nature, associated with uneasiness,
discomfort and concert of fear about some defined or undefined feature
threat. Some degree of anxiety is a part of normal life.Treatment is needed
when it is proportionate to the situation and excessive. Some psychotics
and depressed patients also exhibit pathological anxiety.

4. TYPES OF ANXIETY
• OBSESSIVE –COMPULSIVE DISORDER
• GENERALIZED ANXIETY DISORDER
• PANIC DISORDER
• POST –TRAUMATIC STRESS DISORDER
• SOCIAL PHOBIA( OR SOCIAL ANXIETY DISORDER

5. • OBSESSIVE –COMPULSIVE DISORDER (OCD)
obsessive compulsive disorder OCD is one of the 10 leading causes of disability.
Patient with OCD experience significant impairment in their quality of life (QOL) with A reduction in social
family and occupational functioning.
• GENERALIZED ANXIETY DISORDER
Generalized anxiety disorder , GAD , is an anxiety disorder characterized by chronic anxiety, exaggerated worry
and tension ,even when there is little or nothing provoke it.
• PANIC DISORDER
Panic disorder is an anxiety disorder and is characterized by unexpected and repeated episodes of intense fear
accompanied by physical symptoms
• POST –TRAUMATIC STRESS DISORDER
post – traumatic stress disorder ptsd is an anxiety disorder that can develop after exposure to a terrifying event
or ordinal in which grade physical harm occurred or was threatened.
• SOCIAL PHOBIA
Social anxiety disorder , is an anxiety disorder characterized by overwhelming anxiety and excessive self
consciousness in everyday social situation
CONTINUE…

6. PATHOPHYSIOLOGY
• The significant mediators of anxiety in the central nervous system are thought to be
norepinephrine, serotonin, dopamine, and gamma-aminobutyric acid (GABA). The
autonomic nervous system, especially the sympathetic nervous system, mediates most of the
symptoms.
• The amygdala plays an important role in tempering fear and anxiety. Patients with anxiety
disorders have been found to show heightened amygdala response to anxiety cues. The
amygdala and limbic system structures are connected to prefrontal cortex regions, and
prefrontal-limbic activation abnormalities may be reversed with psychological or
pharmacologic interventions.

7. ANXIETY-INDUCED MODEL
• Elevated Plus Maze (EPM) test
• The apparatus comprise two open arms (16 cm × 5 cm) and two closed arms (16 cm × 5 cm × 10 cm) that
extended from a common central platform (5 cm × 5 cm). The entire maze was elevated to a height of 50 cm
above floor level.
• Group I (negative control) was treated with vehicle.
• Groups II to (test groups) were treated with 4 doses of the plants extracts.
• Group III treated with diazepam, 3 mg/kg ip was used as positive control.
• One hour after treatment, the mice were individually placed on the EPM centre platform facing an open arm
and observed for 5 min. The number of entries by each animal into open or closed arms and the time spent by
each animal in either open or closed arms (conventional parameters) were recorded with stopwatches. The
inner faces of closed arms and the platform were cleaned with alcohol after each mouse recording.

8. AIMTO EVALUATE ANTI-ANXIETY ACTIVITY
OF CYPERUS ROTUNDUS
• Scientific name: Cyperus rotundus
• Family : Cyperaceae
• Higher classification: Nutsedges
• Cyperus rotundus l.: Cyperus rotundus , l.
• Kingdom: Plantae
• Conservation status : least concern ( population stable)
• Rank : Species

9. Extraction and preparation of test sample
• Freshly collected tubers of C. rotundus Rhizome, were washed, shade-dried, powdered, and
soaked in chloroform for 48 h. The resulting extract was filtered, distilled, and extracted with
chloroform. The resulting marc was extracted with alcohol exhaustively and the EECR was
prepared as a suspension in 1% sodium carboxy methyl cellulose (SCMC). It was analyzed by
HPLC and found to have 13 peaks

10. PLAN OFWORK
STEPTAKEN DURATION
LITERATURE SURVEY OCTOBER MONTH
PLANT COLLECTION & AUTHEICATION NOVEMBER-DECEMBER
EXPERIMENT PREPARATION JANUARY-FEBRUARY
ACTIVITY MARCH
THESISWRITINGAND PUBLICATION APRIL

11. REFERENCE
Tripathi, K.D. 2018. Essential of medical pharmacology.8the ed. New Delhi: ,
India: jaypee Brothers medical. Copy citation
Khare, C.P. ( 2007) Indian Medical plants , First Indian Reprint , Springer (india)
pvt.ltd; New Delhi
Soudi, S., Ayissi,E.R, Lakoulo,N.H. Maidawa,F. 2011. Anxiolytic activity
evolution of four medicin plant from Cameroon. Published online 2011 Jul 3.
doi: 10.4314/ajtcam.v8i5S.19