Amino acid sequencing is the process of identifying the arrangement of amino acids in proteins and peptides. Numerous distinct amino acids have been discovered in nature but all proteins in the human body are comprised of just twenty different types.

1. Amino acid
sequencing.

2. WHAT IS AMINO ACID SEQUENCING?
● The arrangement of amino acid in a protein.
● Proteins can be made up of 20 different kinds of amino acids.
● Can be used to compare similarities between species.
● Two species and their common ancestor have similar DNA is
strong evidence supporting evolution.

3. ● All living cell has same basic DNA structure and genetic code.
● Organisms which are fairly similar show more genes in
common .
● Protein also help study the similarities between species.

4. RESEARCH REFERENCE:
Evolution and taxonomy of positive strand RNA virus: implications
of comparative analysis of amino acid.
https://scholar.google.co.in/scholar?q=amino+acid+sequencing+in+taxonomy+research+paper&hl
=en&as_sdt=0&as_vis=1&oi=scholart#d=gs_qabs&u=%23p%3DquyHLiczYjMJ

5. .
The G+C content% in DNA ,
reflects the base sequence
The DNA of both
eukaryotes and
prokaryotic microorganism
varies greatly in G+C
content.
Prokaryotic G+C content is
most variable ranging from
around 25 to 80%
If two organisms differ in
their G+C content by more
than 10% ,their genomes
have quite different base
sequence.
The G+C content of many
microorganism has been
determined

6. Importance:
Confirms taxonomic scheme using other
data.
Characterizing prokaryotic genera .

7. There are several ways to compare proteins.
Indirect methods have been frequently used traditionally
1. ELECTROPHORETIC MOBILITY-
Used to study relationship at species and subspecies level
Migration of charged colloidal particles or molecules under
influence of electric field

8. 2. WESTERN BLOTTING
EMPLOYS ANTIBODIES TO DETECT
PROTEINS IMMOBILIZED ON A
BLOTTING MEMBRANE

9. INDIRECT METHODS FOR COMPARISON OF PROTEINS
1. MASS SPECTROMETRY
RAPID AMINO ACID SEQUENCING
MASS/CHARGE RATIO
PHENYLISOTHIOCYANATE / pITC - MOLECULAR SCISSORS
MAJOR PROBLEM
MASS SPECTRO ANALYSIS

10. EDMAN DEGRADATION:

11. REFERENCES-
1. www.sfu.ca
www.jacksoniammino.com
http:/youtu.be/2F57xjy19fk
1. https://www.clinicalmicrobiologyandinfection.com/article/S1198-
743X(14)63195-5/fulltext
https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.056
994-0
1. https://bio.libretexts.org/Bookshelves/Biochemistry/Book%3A_Biochemistry_O
nline_(Jakubowski)/02%3A_PROTEIN_STRUCTURE/2B%3A_Composition_Seq
uence_and_Conformational_Analysis_of_Proteins/B1._Amino_Acid_Analysis_a
nd_Chemical_Sequencing
4 .www.cancer.gov
5. www.bioinfo.org.cn