Hecho por: Valeria Correa e Isabella Correa

1. Isabella Correa Gil
Valeria Correa Valencia
Universidad Pontificia Bolivariana
III semestre

2. DNA DAMAGE

3. APOPTOSIS AND
CELL CICLE

4. Assess the Induction of DNA damage,
apoptosis and cell cycle perturbation mediate
cytotoxic activity of new 5-aminosalicylate–4-
thiazolinone hybrid derivatives

5. WESTERN BLOT
• reacción entre antígeno y
anticuerpo.
• Separación de proteínas de muestra
y luego se identifican con anticuerpo
específico
• Se hizo para valorar el daño del
material genético generada por la
apoptosis durante el ciclo celular.
Para poder determinar ese daño en
el proceso

6. Apoptosis assay
FACS
clasificación de células individuales activadas
por fluorescencia, que es una técnica de
citometría de flujo

7. Constant-field
gel
electrophoresis

8. Ensayo del ciclo celular
Ioduro de
propidio

9. Induction of DNA damage by HH3 and HH13
compounds.

10. Induction of DNA damage by HH3 and HH13
compounds.

11. Activation of ATM/ATR pathway by HH3 and
HH13 compounds.

12. Induction of apoptosis by
HH3 and HH13.

14. CONCLUTION
We conclude that:
1. From the different laboratory tests at the molecular level, it is possible to identify new
alternatives for treatments in which efficacy is increased and adverse effects are
reduced.
2. With the techniques used in this research, it could be determined which substances are
more specific to attack the different cells of each cancer variant and thus make the
treatments more specific.
3. The study not only of the normal functioning of cells, but also of their variants in the
different pathologies at the molecular level, lay the foundations for an adequate
treatment

15. Isabella´s

16. Valeria´s