During normal development and homeostasis, exosomes play an important role in intracellular communication, enabling bioactive components to be transferred between cells. Thus exosomes are being studied as the markers of disease and as a way to understand disease progression. https://www.creative-biolabs.com/exosome/therapeutic-application-of-exosome.htm

1. Exosomes
Biogenesis
& Therapeutics

2. 01
Evs Types and
Characteristics
Exosomes
Biogenesis
Roles in Normal
Physiology and
Disease
Therapeutics &
Drug Delivery
Contents
02 03 04

3. Evs Types and Characteristics
Extracellular vesicles: biology and emerging therapeutic opportunities[J].
Vesicle
typs
Characteristics
Origin Size Marks Contents
Exosomes
Endolysosomal pathway; intraluminal
budding of multivesicular bodies and
fusion of multivesicular body with cell
membrane
40-120 nm
Tetraspanins (such as
TSPAN29 and TSPAN30),
ESCRT components,
PDCDGIP, TSG101, flotillin,
MFGE8
mRNA, microRNA (miRNA) and other non-
coding RNAs; cytoplasmic and membrane
proteins including receptors and major
histocompatibility complex (MHC)
molecules
Microvesicles
Cell surface; outward budding of cell
membrane
50-1,000 nm
Integrins, selectins, CD40
ligand
mRNA, miRNA, non-coding RNAs,
cytoplasmic proteins and membrane
proteins, including receptors
Apoptotic
bodies
Cell surface; outward blebbing of
apoptotic cell membrane
500-2,000 nm
Extensive amounts of
phosphatidylserine
Nuclear fractions, cell organelles

4. ILV
Fuse with lysosome, which
degrade multivesicular
endosomes (MVE) contents.
①
②
Fuse with plasma membrane
where ILVs liberated into the
extracellular space are
released as exosomes.
①
②
Exosomes
Biogenesis

5. ① ②
③
④
⑤
Extracellular vesicles: biology and emerging therapeutic opportunities[J].
ESCRT-dependent
ESCRT-independent (Ceramide-dependent)
Small GTPases-dependent
SNAREs-dependent
Microvesicles formation
①
②
③
④
⑤
Exosomes Biogenesis

6. Roles in Normal
Physiology and Disease
Extracellular vesicles: biology and emerging therapeutic opportunities[J].

7. Immunotherapeutic Potential of Extracellular Vesicles [J].
Pro-inflamatory cytokine secretior
(facilitate embryo implantation)
Normal tissues Exosomes Intestine or milk
Prostate or
placenta
Trophoblast
Attenuate inflammatory response
Inhibition of cytotoxicity
(facilitate fertilization and
pregnancy)
Roles in Normal
Physiology and Disease

8. Roles in Normal Physiology and Disease
B cell Exosomes with
MHC class ll-peptide complexes
Primed CD4+ T cell
Infected macrophage Exosomes DC
B cell
exosomes
Macrophage
exosomes
01
02

9. Mature /Immature /
modified /pulsed DC
Exosomes with
MHC class l or ll-peptide
complexes
Primed T cell
DC
Naive CD8+ or CD4+ T cell
Activation
Antigen
transfer
Immunotherapeutic Potential of Extracellular Vesicles [J].
DC exosomes
03
Roles in Normal Physiology and Disease

10. Tumor cell Exosomes
Exosomes with
tumor antigens
Tumor antigen-
presenting DC
Cytotoxic T
lymphocyte
Cytotoxic CD8+ T
cell activation
Inhibition of cytotoxic activity
T cell apoptosis
Immune suppression
Induce differentiation ofmyeloid-
derived supressor cells (MDSCs)
Inhibition of DC differentiation
Roles in Normal
Physiology and Disease
Immunotherapeuti
c Potential of
Extracellular
Vesicles [J].

11. Exosomes as Therapeutic Targets
①
① Inhibiting extracellular vesicle production
②
② Inhibiting extracellular vesicle release
③
③ Targeting extracellular vesicle components and inhibiting uptake
Extracellular vesicles: biology and emerging
therapeutic opportunities[J].

12. Exosomes as
Therapeutic
Targets
Extracellular vesicles: biology
and emerging therapeutic
opportunities[J].

13. Exosomes for Drug Delivery
Extracellular vesicles: biology and emerging therapeutic opportunities[J].

14. Creative Biolabs Services
EXOSOME SAMPLING
u Exosome Isolation
u Exosome Purification
u Exosome Characterization
u Exosome Quantification
u Exosome Profiling
u Exosome Proteomics Services
u Exosome Lipidomics & Metabolomics Services
EXOSOME ANALYSIS
EXOSOME MANUFACTURING
u Exosome Engineering
u Exosome Manufacturing
EXOSOME-BASED APPLICATIONS
u Exosome Antibody Development
u Exosome Display

15. Web:
www.creative-biolabs.com
Email:
info@creative-biolabs.com
Tel:
1-631-381-2994
Address:
45-1 Ramsey Road, Shirley, NY 11967, USA
Fax:
1-631-207-8356