The students presented on the anti-fibrotic effects of azithromycin (AZT) on lung fibroblasts from patients with idiopathic pulmonary fibrosis (IPF) compared to controls. They evaluated AZT's effects using RT-qPCR, western blot, ELISA, and microarray assays. The results showed that AZT reduces expression of pro-fibrotic genes in IPF fibroblasts more than in control fibroblasts. This suggests AZT has enhanced anti-fibrotic effects on IPF fibroblasts.

1. Presented by the students:
Gabriela Arenas Ramos
Laura Sofía Aristizábal Pérez
III Semester of Medicine
Universidad Pontificia Bolivariana

2. αAzithromycin (AZT)
Macrolide
Anti-inflammatory and
immunomodulatory
properties
Anti-fibrotic
effects
Inhibit bacterial protein synthesis
50S large ribosomal unit
Viral respiratory
infections
STDs

3. Idiopathic pulmonary fibrosis (IPF)
Is a interstitial lung disease cause by abnormal activation of alveolar epithelial cells.
Increase extracellular matrix, because the fibroblast deficiency of autophagy.
Dyspnea, cough and respiratory failure.

4. Objective
Evaluate if αAzithromycin has enhanced
effects on lung fibroblasts from idiopathic
pulmonary fibrosis (IPF) patients
compared to controls-FB.

5. MÉTODO
RT-qPCR
Es usada comúnmente para cuantificar
cambios en la expresión génica ya que
detecta los niveles de ARNm.
Variabilidad de las proteínas.

6. MÉTODO
Western blot
Reacción antígeno - anticuerpo
Electroforesis e identificación por anticuerpos.
Fibronectina, αSMA, LC3 AB (I/II), p62 y
ATP6V1B1.

7. MÉTODO
Elisa
Reacción antígeno-
anticuerpo
Solución coloreada:
+intensidad +cantidad
Procolágeno Iα1

8. MÉTODO
GeneChip microarray assay
Se utiliza para determinar la expresión de muchos genes a la vez.
El apareamiento de las bases complementarias produce una cantidad de luz que se puede
medir.
Determinar genes y vías que están involucradas en los FB de control después del
tratamiento con azitromicina.

9. Resultados

13. Discussion
Tsubouchi K, et al "Our study shows that AZT has anti-
fibrotic and pro- apoptotic effects on
primary fibroblasts and that these effects
are enhanced in IPF fibroblasts
compared to control fibroblasts. We
confirmed that AZT reduces gene
expression levels of pro-fibrotic genes
after stimulation with TGF-β in vitro in
control as well as in IPF fibro- blasts as
recently reported"
Gladue RP, et al “AZT has been described to highly
accumulate in tissues and fibroblasts”
Wuyts WA, et al “AZT has been used for over 30
years as an antibiotic without
significant adverse health effects”

14. Conclusions
1. Report different treatment possibilities.
2. Knowledge of the different cellular
processes that are present in normal
conditions or cause damage.
3. Identify the genes, proteins and molecules
that are present in an exacerbated way in
some pathologies and which are their
normal values.