seminario biologia molecular

1. Clinical cases, drug resistance, and
virulence genes profiling in
Uropathogenic Escherichia coli

2. INTRODUCTION
Like all Gram - bacteria, the E.
coli shell consists of three
elements: the cytoplasmic
membrane, the outer membrane
and, between them, a periplasmic
space consisting of peptide-
glucan.
E. coli is a mesophilic
bacterium, its optimal
development is in the
environment of the body
temperature of warm-blooded
animals (35-43 ° C)
In adhesion is determined by the
presence of fimbriae, which
provide the cells with the ability
to specifically bind to a cellular
receptor.

3. VIRULENCE
RESISTANCE
First mechanism
● Initial adhesion to the
enterocyte.
● Translocation of intracellular
signals.
● Intimate bacterial adhesion.
Second mechanism
the polymerization, long chains of actin
are formed that alter the morphology of
the cytoskeleton, the microvilli are
damaged and they lose their function
● Beta lactams
● Quinolones
● Tetracyclines
● Chloramphenicol
● Trimethoprim-Sulfamethoxazole

4. OBJECTIVE
Detect and report the presence or absence of
virulence factors and specific gene profiles
(CnF1, Upah, hlyA, Ibea, and CdtB) in isolated
pathotypes of patients with urinary tract
infections in Shahr-e-Qods, Iran, for the first
time.

5. MATERIALES Y MÉTODOS
test de susceptibilidad antimicrobiana
Se basa en la susceptibilidad de
bacterias a ciertos antibioticos. Una
vez preparado el cultivo con el
antibiotico, nos da como resultado, un
diámetro de halo de inhibición.
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Los aislamientos se probaron en
agar MuellerHintone (Merck,
Alemania) contra 11 discos
antibióticos (Padtan-Teb, Irán).la
cepa de E. coli de ATCC 25922 se
usó como el caso de control
para ensayo antibiograma

6. PCR PERFOMANCE
FUNDAMENTO
Reacción enzimática in vitro que
amplifica millones de veces una
secuencia específica de DNA.Se usa
ADNc proveniente del ARNm se le
conoce como RTPCR por medio
transcriptasa reversa.
UTILIDAD
Amplificar genes de virulencia cnf1, upaH, hlyA,
ibeA y cdtB por los genes relacionados
imprimaciones (Cinnagen-Irán).

7. results

10. DISCUSION
Jahandeh et al. 2015; Behzadi
and Behzadi 2017; López-
Banda et al. 2014).
The more pathogenic strain, the
more genomic content. This
characteristic of E. coli strain
depicts the plasticity of the
genome which gives it a
diversity of pathogenesis
AUTHOR CONCEPT YES OR NOT
reported a range of 70 –86%
prevalence rate of UpaH
virulence factors among E.
coli strains
Allsopp et al. (2010)
some virulence genes
including hly and cnf1 that
encode toxins may be
recognized on the same
pathogenicity islands (PAIs)
or in the form of plasmid
operons
Jahandeh et al. 2015;
Paniagua-Contreras et al.
2017; Tiba et al. 2008)

11. CONCLUSION
Despite the fact that the
pathotype genes were in the
enterobacteria, there is a
prevalence of infections where
these patotypes are not yet
related
It would be very good for
Colombia to search for
patotypes to improve the
treatment of patients to manage
the specific antibiotic treatment
and reduce bacterial resistance.

12. genes