Seminar ppt on microfluidics

1. MICROFLUIDICS

2. INTRODUCTION
Microfluidics is the technology of fluid manipulation in channels
with dimensions of tens of micrometers; it has emerged in
recent years as a distinct new area of research thanks to its
application in many diverse fields, such as chemistry, biology,
medicine, and physical sciences.

3. 1.WHAT IS A
MICROFLUID ?
 It is the science and technology of
systems that process or manipulate
small amounts of fluids.
 Using channels with dimensions of tens
to hundreds of micrometers.
 The picture shown here is an example
of microfluids in human body, lung
alveoli.

4. 2.USES OF MICROFULIDICS
Microfluidics are generally used in the following sections:
 DNA chips.
 Molecular biology.
 Optics.

5. 2.1 DNA CHIPS (microarrays)
 Is a miniature array where a multitude of different capture
agents, most frequently monoclonal antibodies, are
deposited on a chip surface.
 They are used to determine the presence or the amount of
proteins in biological samples.
 Example ; Amount of proteins in blood.

6. 2.2 MOLECULAR BIOLOGY
 In addition to microarrays, biochips have been designed for
two-dimensional electrophoresis, transcriptome analysis,
and PCR (Polymerase chain reaction)amplification.
 Also used in cell separation , protein analysis and
microorganism capturing.

7. 2.3 OPTICS
 The merging of microfluidics and optics is typical known as
opto-fluidics.
 Example of opto-fluidic devices is a microscope.
 Microfluidic flow enables fast sample throughput, automated
imaging of large sample populations, as well as 3D
capabilities

8. 3.BRANCHES OF MICROFLUIDICS.
1.Continuos flow microfluidics.
2.Digital microfluidics.
3.Acoustofluidics.

9. 3.1 CONTINUOUS FLOW
MICROFLUIDICS
 Continuous flow microfluidics enable to manipulate
continuous flow of liquid through micro-channels.
 Using devices such as a external pressure pumps or integrated
mechanical micro-pumps.
 CFM is used in a wide range of applications like in chemical
,energy and enviornmental fields.

10. 3.2 DIGITAL MICROFLUIDICS
 It is also called droplet microfluidics or emulsion science.
 It enables to manipulate autonomous droplets on a substrate
using electro-wetting.
 Droplets generation can be used in a largescale of applications
like in : Synthesis of nanoparticles Single cell analysis.

11. 3.3 ACOUSTOFLUIDICS
 It refers to the study of acoustic waves on microscale to
nanoscale fluidics environment.
 Acoustofluidics mainly deals with the use of acoustic fields.
 Mainly in ultrasonics int fluids within microfluidic channels
allowing to manipulate cells and particles.

12. 4 LOW-COST MICROFLUIDICS
1.Paper-based microfluidics.
2.Plastic-based microfluidics.
3.Textile-basedmicrofluidics.

13. 4.1 PAPER-BASED MICROFUIDICS
 Easily available and cheap.
 Low fabrication cost.
 Thin and lightweight.
 Disposable and biodegradable.

15. 4.2PLASTIC-BASED MICROFLUIDICS
 Use of materials such as polydymethilsiloxane
(PDMS),acrylic(PMMA),Polystyrene.
 Used in variety of applications that cannot be achieved through
paper-based.
 Can be used repetitively.
 It is affordable and user friendly.
 It is sensitive.

16. 4.3 TEXTILE-BASED MICROFLUIDICS
 Applied in sports performance measurement such as realtime
sweat pH monitoring.
 Can be connected via Bluetooth for real-time analysis on
smartphones.
 Used in shirts and ESP for security forces.

17. TEXTILE-BASED MICROFLUIDICS USES:

18. CONCLUSION
This presentation focuses on applications where microfluidics
can enhance experimental sensitivity and throughput,
particularly in recent development in single-cell analyses and
analyses on multi-cellular or complex biological entities. We
conclude that microfluidics is not necessarily always useful for
systems biology, but when used appropriately can greatly
enhance experimentalists’ ability to measure and control, and
thereby enhance the understanding of and expand the utility
of biological systems.

19. REFERENCES
 Microfluidics in Systems Biology – Hype or Truly Useful? - PMC (nih.gov)
 Microfluidics - an overview | ScienceDirect Topics
 Microfluidics applications: A short review – Elveflow
 Frontiers | Microfluidics for Biotechnology: Bridging Gaps to Foster
Microfluidic Applications (frontiersin.org)

20. THANK YOU