5. Complex vessel system model
(microvasculature)
Simple vessel system model
(straight channel)
Microfluidic Systems Simulate Architecture of
Drug Delivery Path
5
6. Mimicking In Vivo Conditions through Microfluidics
channel coating
crystal
3µm
6
0.02 µm
9. 9
Amount of Folic Acid (mg)
Amount of Folic Acid Remaining
0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9
Quantitative Data Shows Shear Forces in All Devices
Complex A
Complex B
Straight A
Straight B
Straight C
Stock
11. Acknowledgements
■ Izzy Jarvis and Dr. Samir Mitragotri
■ Supermentors: Dr. Stephanie Mendes
and Dean Morales
■ SIMS Coordinator: Mary Alice Callaghan
■ Resident Advisors: Rachel Alvelais,
Stephen Chih, Marine Minasyan, and
Joseph Sanz
11
Editor's Notes
Megan:
-current cancer therapies work by introducing toxins to cells
-healthy cells are harmed in the process
-targeted therapy is seeking out only cancerous cells
-explain analogy
Ekta:
-introduce 3 parts of drug
**crystal itself is made of the drug, current methods are mostly attaching the drug to another compound/particle
-camptothecin is a drug that prevents breast cancer cells from reproducing
-polymer serves 2 purposes: 1) allows crystal to travel through the body w/out being detected by immune system 2) links folic acid to carrier
-Breast cancer cells, cell takes in drug preventing replication have folic acid receptors, folic acid takes carrier to those cells
-scaffold can be modified
Celeste
Youssef Sibih:
-flowing carriers through microfluidic devices -> describe different devices
-have found that shearing forces rip polymers off of carrier -> problematic bc polymers are linkers and carriers need them to travel
-hypothesis: architecture does NOT effect amount of polymer that falls off, only due to shearing force
-goal: send carriers through both, quantitatively compare amount of polymer that comes off
Celeste: **ask what absorbance/fluorescence device is called
Attached tubes to flow carriers through
Coated tubes to mimic the extracellular matrix inside veins
Incubated devices to match vein temperature
Used standard curve/absorbance/fluorescence to determine drug concentration because we wanted to dilute to 100 mg/mL
The pump shown pushes the fluid through the devices at 4 mL/min
