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LIST OF GLASS WARE
USED IN BIOCHEMISTRY
LABORATORY
Erlenmeyer
Flask
Griffin Beaker
Approximate Graduations
Erlenmeyer flasks are often used as
reaction vessels, particularly in
titrations. As with beakers, the
volume markings should not be
considered accurate.
Beakers are used as containers. They
are available in a variety of sizes.
Although they often possess volume
markings, these are only rough estimates
of the liquid volume. The markings are
not necessarily accurate.
Used for Routine Volume Measurements of 25 mL or Greater
Graduated Cylinder
Graduated cylinders are
used to transfer liquids
with accuracy.
PIPETTES
Graduated pipette
Volumetric pipettes
Pipette filler
Rubber bulbs
Blow out pipette
Wide mouth pipette
Pasteur pipette Transfer pipette
• Volumetric flasks are used to measure and
store solutions with a high degree of accuracy.
• These flasks generally possess a marking
near the top that indicates the level at which
the volume of the liquid is equal to the volume
written on the outside of the flask.
• These devices are often used when solutions
containing dissolved solids of known
concentration are needed.
Volumetric flask
Discharge all air
A burette is a graduated glass tube with a tap at
one end, for delivering known volumes of a
liquid, especially in titrations. It is a long,
graduated glass tube, with a stopcock at its
lower end
Burette clamp
BURETTE
MEASURING A LIQUID
• Position of eye at all volumetric
vessels must be at the same level
as the meniscus.
• If not, the parallax will cause an
error while reading the position of
the meniscus of a liquid in a
burette.
• It will be a positive mistake if the
eye is lower, and negative if the
eye is higher than the meniscus
plane.
• In a laboratory a condenser is a piece of
laboratory glassware used to cool hot
vapors or liquids.
• A condenser usually consists of a large
glass tube containing a smaller glass
tube running its entire length, within
which the hot fluids pass.
• The outer glass tube usually has two
hose connections, and a coolant (usually
tap water or chilled water/anti-freeze
mixture) is passed through it.
CONDENSOR
• A Separatory funnel is also known
as separation funnel, separating
funnel, or colloquially sep funnel is a
piece of laboratory glassware used
in liquid-liquid extractions to
separate the components of a
mixture into two immiscible solvent
phases of different densities.
SEPARATORY
FUNNEL
Funnel Test tube Test tube
holder
Test tube
stand
Glass rods Spatula
Petri dish Watch glass Mortar and
pestle
Dessicator Crucible Reagent

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Glass ware used in the laboratory.pptx

  • 1. LIST OF GLASS WARE USED IN BIOCHEMISTRY LABORATORY
  • 2. Erlenmeyer Flask Griffin Beaker Approximate Graduations Erlenmeyer flasks are often used as reaction vessels, particularly in titrations. As with beakers, the volume markings should not be considered accurate. Beakers are used as containers. They are available in a variety of sizes. Although they often possess volume markings, these are only rough estimates of the liquid volume. The markings are not necessarily accurate.
  • 3. Used for Routine Volume Measurements of 25 mL or Greater Graduated Cylinder Graduated cylinders are used to transfer liquids with accuracy.
  • 4. PIPETTES Graduated pipette Volumetric pipettes Pipette filler Rubber bulbs Blow out pipette Wide mouth pipette
  • 6.
  • 7. • Volumetric flasks are used to measure and store solutions with a high degree of accuracy. • These flasks generally possess a marking near the top that indicates the level at which the volume of the liquid is equal to the volume written on the outside of the flask. • These devices are often used when solutions containing dissolved solids of known concentration are needed. Volumetric flask
  • 8. Discharge all air A burette is a graduated glass tube with a tap at one end, for delivering known volumes of a liquid, especially in titrations. It is a long, graduated glass tube, with a stopcock at its lower end Burette clamp BURETTE
  • 9. MEASURING A LIQUID • Position of eye at all volumetric vessels must be at the same level as the meniscus. • If not, the parallax will cause an error while reading the position of the meniscus of a liquid in a burette. • It will be a positive mistake if the eye is lower, and negative if the eye is higher than the meniscus plane.
  • 10. • In a laboratory a condenser is a piece of laboratory glassware used to cool hot vapors or liquids. • A condenser usually consists of a large glass tube containing a smaller glass tube running its entire length, within which the hot fluids pass. • The outer glass tube usually has two hose connections, and a coolant (usually tap water or chilled water/anti-freeze mixture) is passed through it. CONDENSOR
  • 11. • A Separatory funnel is also known as separation funnel, separating funnel, or colloquially sep funnel is a piece of laboratory glassware used in liquid-liquid extractions to separate the components of a mixture into two immiscible solvent phases of different densities. SEPARATORY FUNNEL
  • 12. Funnel Test tube Test tube holder Test tube stand Glass rods Spatula
  • 13. Petri dish Watch glass Mortar and pestle Dessicator Crucible Reagent