1. David G. Barton
dgbarton27@gmail.com
902 North Dr.
Blacksburg, Va. 24060
(540) 239-9375
(540) 961-0235
Qualifications
 B.S. Biology, Virginia Tech (2014)
 Minor in Turfgrass Management
 Minor in Entomology
 Associates Degree in Science, New River Community College (2012)
 Excellent mechanical aptitude
 Experience supervising, motivating, and organizing team projects
 Experience with laboratory work, PCR, aseptic technique, inoculation, isolation, and
identification of microorganisms, chromatography, fluorometry, spectrophotometry, and
gravimetric and volumetric analysis
 Experience preparing, maintaining, and propagating plant specimen
 Experience with field work and preparation of reports
 Experience preparing tables and graphs from imported data in Excel, and JMP
 Attention to detail
 Follows instructions according to established procedures, and GLP
Work Experience
• Intrexon Corporation Ultra Vector Division (August 2014-March 2015)
o Lab Associate
o Experimental Design
o DNA Assembly, and Ligation (Gibson Assembly and Restriction Digest)
o DNA Isolation and Preparation
o PCR, TD-PCR, Hot Start PCR, cPCR
o Preparation of Plasmid DNA by Alkaline Lysis with SDS and Ion Exchange
Chromatography (Mini, Midi, Maxi, Mega and Giga)
o Transformation of Competent and Electrocompetent E. Coli
o Electroporation
o Sanger Sequencing (BigDye) and NGS Sequencing (Illumina)
o Microtiter Diagnostic Restriction Digest
o Gel Electrophoresis and Gel Analysis
o Biological Robot Operation (Hamilton, Tecan, QiaCube)
o Gel Purification of Fragments
o Lentiviral Growth and Plasmid Maxi-Prep
o DNA Library Inventory Management (Hamilton BioStore)
o Media Preparation and Autoclave Operation
• Virginia Tech Turfgrass Research Center (Summer 2013)
o Laboratory and Field Technician
o Plot Preparation, Treatment and Maintenance to mimic golf course turf
conditions in order to test commercial pesticide formulations

2. o Plot preparation for NTEP seed variety trials
o Plot treatment of various pesticides
o Equipment maintenance and Spray equipment calibration
o Relative qualitative color grading and Turf disease symptom identification
o Moisture and soil density readings
o Soil sample processing and size separation
o Data Collection
• Valley Landscaping (Summers of 2005-2012)
o Equipment Maintenance
o Landscape Maintenance
o Crew Supervisor
o Supervised, motivated, and organized a team of 4 laborers to complete multiple
daily projects