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http://www.linkedin.com/in/vivianypeng
Vivian Y Peng
Summary
Experienced in scientific project and laboratory management with extensive experience in
experimental design, conducting research, project mentoring, and lab and field research logistics
Skills
• Project Planning and Management, Resource Management, Requirements Building, Project,
Execution, Process Creation and Management, Protocol and User Training, Analysis, Documentation
of Standard Operating Procedures, Schedules, Crisis Management, Conflict Resolution,
• Group Presentations, Client Support, Proficient in Verbal and Written Communication, Field and
Project Logistics
• Proficient in MS Office, MS Access, Adobe Acrobat, PhotoShop, Saas (ie. Altassian, Kanban, Harvest,
Google Apps)
Molecular Techniques:
• DNA sequencing, genomic DNA isolation, purification of plasmid vectors (Qiagen and Solid Phase
Reversible Isolation), cloning and sub-cloning of genomic sequences, Southern Blot, DNA ligation,
DNA extraction, agarose gel electrophoresis, DGGE gel electrophoresis, RNA Isolation, reverse and in
vitro transcription, Ribosome subtraction, small RNA manipulation, expression vector construction,
next gen sequencing.
• Western Blot, protein extraction, isolation, purification, and characterization, and quantitative assays
(i.e. Bradford-Biorad, Lowry-Phenol Method, Gel Filtration, protein kinetics).
• PCR fingerprinting (LH-PCR, ARISA), , PCR cloning, reverse transcription-PCR (RT-PCR), primer
design using Geneworks, DS Gene/MacVector, and Big Dye Terminator sequencing reactions.
• Quantitative PCR (TAQMAN, SYBR Green I), qPCR probe synthesis
• Murine husbandry
• Bacterial: Bacterial transformation, bacterial genetics, and culture media preparation. Fungal: Fungal
transformation, fungal genetics, lipid extraction, and spore crossing and scoring. Cell Staining (DAPI,
Proflavin, FISH), cell enumeration.
• Plant growth and maintenance, sample collection, plant tissue culture, media preparation, and
regeneration of shoots from leaf discs and calluses.
Microarray Techniques:
• Affymetrix Gene Chip System - Proficient in target labeling, chip hybridization, and GeneChip system
operation for both gene expression and exon arrays.
• Spotted Array Platform - Proficient in array printer [DeRisi v 2.0 arrayer] operation, slide blocking,
hybridization, and washing. Proficient in the operation of the GenePix 4000b Scanner (Molecular
Devices) and the Agilent Array Scanner.
• Array Related Software - Proficient in using Stratagene/Iobion GeneTraffic 2.0 database and
Bioconductor software.
Instrumentation:
• Operation of gas chromatography systems (HP 6890) and software (HP Chem Station); high
performance liquid chromatography systems (Waters Breeze HPLC) and software (Waters
Millennium); liquid scintillation counters, automated sequencers (MegaBACE-Molecular Dynamics,
3500 Genetic Analyzer-Life Technologies), liquid handlers (Beckman Coulter Biomek2000,
Multimek96), and DNA microarray spotters.

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http://www.linkedin.com/in/vivianypeng
• Operation and maintenance of Olympus confocal {Fluoview1000] and epifluorescence microscope
system.
• Operation and maintenance of Life Technologies StepOne Plus and 7500 RT-PCR system
• Operation and maintenance of Molecular Devices Gemini EM, Gemini XPS, SpectraMax190 systems
and associated software, SoftMaxPro
• Operation and maintenance multichannel peristaltic pumps, Millipore Tangential Flow Filtration System
• Proficient in CLC Genomics Workbench, Sequencher, DSGene, R, qPCR Primer / probe design
• Microsoft Visual Basic® 6.0, XML, DHTML, HTML, Microsoft Office XP, Microsoft Windows® 2000,
Adobe PhotoShop and PageMaker, Macromedia Freehand.
Professional Experience
Account/Project Manager – PalominoDB (now Blackbird)
Verdi, Ca 2012 – 2013
• Worked with technical staff of two to three to provide and document recommendations via service
delivery plans and managed that delivery
• Coordinated tasks across technical staff located globally.
• Managed four internal projects resulting in improved client service
• Worked with team members to ensure proper communication and process around reports, budgets,
hours and regular communications
• Supported eight clients through service delivery; in both planning & execution
• Onboarded three new clients and performed periodic account reviews with seven existing clients
• Help create three processes for task and project management within the PMO
• Created Change Management process for tracking Change Requests on projects
• Created Project Transition process to track Project Ownership change between groups
• Copy editor for client recommendation documentation
• Worked efficiently and effectively in a 100% remote position
Staff Microbiologist – Desert Research Institute, Reno,NV 2007 – 2013
• Free-drifting icebergs: a proliferating dispersion sites of iron enrichment, organic carbon production
and export in the southern ocean
- Field Scientist: Participant in a twenty seven member research team that is responsible for, sample
collection and experimental design and execution
- Conducted research to describe the marine baterioplankton community structure of the water column
under influence of large tabular icebergs, using molecular tools and culture experiments.
• International Polar Year Project : Bacterioplankton Genomic Adaptation to Antarctic Winter
- Field Scientist: Participant in an eight member research team that is responsible for fields laboratory
operations, sample collection and experimental design and execution
-Conducted research to describe the bacterioplankton community off shore of Palmer Station,
Antarctica in the austral winter.
- Assayed for gene expression of targeted species and functional genes related to nitrogen and
carbon cycles.
• Dry Lakes : Lake Vida Genomics 2005, 2010
• - Field Research Logistics: Responsible for purchasing and shipping of scientific equipment and
supplies into remote field sites (ie: Antarctica).
-Contributed to the project by generating data used in an unpublished manuscript.
- Managed the logistics for the 2010 field trip back to Lake Vida.
• Lab Manager: Supervised and aided three graduate and two undergraduate students in their own
projects and projects in the lab.

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• Managed lab chemical inventory, purchasing, logistics and operations.
Staff Research Associate III- Supervisor – University of California, Berkeley, Berkeley,
CA 1999 - 2007
• Setup and managed the daily operations of the Functional Genomics Laboratory, a microarray core
facility specializing in genomics technologies.
• Optimized user protocols for high throughput PCR and cDNA library preparation, RNA isolation,
sample amplification, and array hybridization conditions.
• Established quality control metrics for array design and data analysis in spotted array and Affymetrix
GeneChip platforms.
• Managed ten production teams that created spotted arrays starting from cDNA libraries (bacterial
glycerol stocks) to finished array product.
• Trained thirty facility users in sample preparation, sample labeling, and robotics usage (liquid handlers
and microarray printer use).
• Performed sub-contract work specializing in RNA isolation, sample labeling, QC analysis of array data,
and first-pass data analysis in array platforms.
• Managed three technicians in tasks associated with the facility and in their own research projects.
• Supervised and aided four graduate students in their own projects and projects in the lab.
• Maintained facility website.
Staff Research Associate I – Joint Genome Institute, LBNL,
Walnut Creek, CA 1998-1999
• Performed high throughput library creation (cloning genomic fragments into a common vector),
plasmid prep, capillary sequencing, and genomic data alignment as part of the Human Genome
Sequencing Project.
Physical Science Aide – United States Department of Agriculture, Albany, CA
1997-1998
• Evaluated the effects of nitrogen and phosphorus concentrations on Neurospora Crassa growth and
fatty acid composition.
Education
University of California, Berkeley, Berkeley, CA
B.A. Biochemistry and Molecular Biology
Areas of Concentration: Biochemistry, Molecular Biology, Genetics
Publications and Web Resources
• Kuhn, E, Ichimura A, Peng V, Fritsen C, Trubl G, Doran P, and Murray, AE, "Brine assemblages of
ultra-small microbial cells within the ice cover of Lake Vida, Antarctica." AEM 2014 Accepted
• Murray, AE, F Kenig, CH Fritsen, CP McKay, KM Cawley, R Edwards, E Kuhn, DM McKnight, NE
Ostrom, V Peng, A Ponce, JC Priscu, V Samarkin, AT Townsend, P Wagh, SA Young, PT Yung, PT
Doran. (2012). Microbial life at -13°C in the brine of an ice-sealed Antarctic Lake. Proc. Natl. Acad.
Sci. USA. 2012; 109:20626-20631. doi:10.1073/pnas.1208607109
• Murray, AE, Peng V, Tyler C, Wagh P. Marine baterioplantkon biomass, activity and community
structure in Antarctic icebergs. Deep Sea Research, II. doi:10.1016/j.dsr2.2010.11.021 , 2011
• The Cancer Genome Atlas Network, Comprehensive genomic characterization defines human
glioblastoma genes and core pathways. Nature 455, 1061-1068 (23 October 2008).
doi:10.1038/nature07385

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• Yang YH, Dudoit S, Luu P, Lin DM, Peng V, Ngai J, Speed TP. Normalization for cDNA microarray
data: a robust composite method addressing single and multiple slide systematic variation Nucleic
Acids Res. 2002 Feb 15;30(4):e15.
• Lin DM, Yang YH, Scolnick JA, Brunet LJ, Marsh H, Peng V, Okazaki Y, Hayashizaki Y, Speed TP,
Ngai J. Spatial patterns of gene expression in the olfactory bulb. Proc Natl Acad Sci U S A. 2004 Aug
24;101(34):12718-23.
• IPY: http://genex2.dri.edu/
• Free drifting Iceberg project: http://iceberg.dri.edu/
• http://microarrays.berkeley.edu