Detection of Gene HLA-B5801 on A Lateral-Flow Membrane
1. Abstract of Applied Sciences and Engineering, 2016, Vol.6
DOI: 10.18488/journal.1001/2016.6/1001.6
6th
International Scientific Conference on Applied
Sciences and Engineering
26-27 January, 2016
Bayview Hotel Singapore
Conference Website: www.scihost.org
8
Paper ID: 26/16/ 6
th
ISCASE
Detection of Gene HLA-B5801 on A Lateral-Flow Membrane
Jui-Chuang Wu1
--- Yue-Je Jung2
1,2
Department of Chemical Engineering, Chung Yuan Christian University, 200 Chung Pei Rd.,
Chung Li, Tao Yuan, Taiwan, R.O.C.
Abstract
In this study, a sequence-specified DNA segment was detected on a porous polymer
surface, incorporated with an immune reaction and a color development by nano-size
gold particles. The Human leukocyte antigen (HLA) B*5801 DNA, gene of Arabidopsis
thaliana, Pda13015, human gene PSMA5, and Avian-Influenza (AI) gene H5 were
individually detected on the membrane-based lateral-flow strips. HLA-B5801 was
determined a detection limit as 1.2 ng, and with a good selectivity over other species
genes.
Keywords: Gene HLA-B5801, Lateral flow, Nitrocellulose membrane, Biosensors