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Abstract of Applied Sciences and Engineering, 2016, Vol.6
DOI: 10.18488/journal.1001/2016.6/1001.6
6th
International Scientific Conference on Applied
Sciences and Engineering
26-27 January, 2016
Bayview Hotel Singapore
Conference Website: www.scihost.org
8
Paper ID: 26/16/ 6
th
ISCASE
Detection of Gene HLA-B5801 on A Lateral-Flow Membrane
Jui-Chuang Wu1
--- Yue-Je Jung2
1,2
Department of Chemical Engineering, Chung Yuan Christian University, 200 Chung Pei Rd.,
Chung Li, Tao Yuan, Taiwan, R.O.C.
Abstract
In this study, a sequence-specified DNA segment was detected on a porous polymer
surface, incorporated with an immune reaction and a color development by nano-size
gold particles. The Human leukocyte antigen (HLA) B*5801 DNA, gene of Arabidopsis
thaliana, Pda13015, human gene PSMA5, and Avian-Influenza (AI) gene H5 were
individually detected on the membrane-based lateral-flow strips. HLA-B5801 was
determined a detection limit as 1.2 ng, and with a good selectivity over other species
genes.
Keywords: Gene HLA-B5801, Lateral flow, Nitrocellulose membrane, Biosensors

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Detection of Gene HLA-B5801 on A Lateral-Flow Membrane

  • 1. Abstract of Applied Sciences and Engineering, 2016, Vol.6 DOI: 10.18488/journal.1001/2016.6/1001.6 6th International Scientific Conference on Applied Sciences and Engineering 26-27 January, 2016 Bayview Hotel Singapore Conference Website: www.scihost.org 8 Paper ID: 26/16/ 6 th ISCASE Detection of Gene HLA-B5801 on A Lateral-Flow Membrane Jui-Chuang Wu1 --- Yue-Je Jung2 1,2 Department of Chemical Engineering, Chung Yuan Christian University, 200 Chung Pei Rd., Chung Li, Tao Yuan, Taiwan, R.O.C. Abstract In this study, a sequence-specified DNA segment was detected on a porous polymer surface, incorporated with an immune reaction and a color development by nano-size gold particles. The Human leukocyte antigen (HLA) B*5801 DNA, gene of Arabidopsis thaliana, Pda13015, human gene PSMA5, and Avian-Influenza (AI) gene H5 were individually detected on the membrane-based lateral-flow strips. HLA-B5801 was determined a detection limit as 1.2 ng, and with a good selectivity over other species genes. Keywords: Gene HLA-B5801, Lateral flow, Nitrocellulose membrane, Biosensors