Creative BioMart has more than 20 years of experience in protein characterization. We have extensive experience in developing and establishing protein identification methods to help our customers generate the data they need to obtain the level of product identification required for regulatory submission. These characterization services are also part of our stability and product launch support services, where we provide critical identity, purity, and performance measurements.

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Protein Characterization
Creative BioMart

2. Introductions
Proteins are complex molecular entities derived from
biological processes. Their size, molecular structure and
physical and chemical properties are different from each
other. These differences allow the analysis and
characterization of proteins through separation and
identification.
Creative BioMart has more than 20 years of experience in
protein characterization. We have extensive experience in
developing and establishing protein identification methods
to help our customers generate the data they need to obtain
the level of product identification required for regulatory
submission. These characterization services are also part of
our stability and product launch support services, where we
provide critical identity, purity, and performance
measurements.
B

3. Protein modification
One of the important fields of protein characterization is to
identify those proteins and their modification sites that are
post-translationally modified, and to characterize the modified
functions. Almost all proteins, whether produced and purified
as recombinant proteins or isolated from natural sources, will
carry modified amino acids to some extent.
At Creative BioMart , we use a mass spectrometer to detect the
smallest and most annoying modifications. Usually, we
combine electrophoresis, HPLC, and peptide mapping methods
for analysis. Generally, mass spectrometry data is qualitative
rather than quantitative. However, we will provide you with
data assessments-that is, real numbers about the degree of
modification that occurs in the protein-so that you can clearly
understand how the protein works.

4. In the production process, they are almost always not
completely purified and homogeneous molecules, but show a
certain degree of structural heterogeneity. This means that the
characterization of proteins always has three aspects:
 • Identification of main protein components
 • Identification of minor ingredients
 • Quantification of minor ingredients
Protein analysis
Creative BioMart focuses on a wide range of
analytical testing technologies to support the
characterization and development of
recombinant protein and peptide therapeutics.
Our team of experts, coupled with advanced
technology, supports customers while
maintaining the highest standards of quality,
technical analysis and reporting.

5. Amino acid analysis
The amino acids can be derivatized with ninhydrin or o
‐phthalaldehyde to make fluorescent derivatives that are easy
to detect.
These are chromatographed by reverse‐phase HPLC (high
pressure liquid chromatography).
 The characteristic retention times are used to identify the a
mino acids.
 The fluorescence level can be quantified to determine the
amount of that amino acid.

6. Determination of Primary Structure
Hydrolyze peptides with hot 6M HCl.
– Usually done by chromatography
Identify the N‐term and C‐term Aas
– C‐term via carboxypeptidase
– N‐term via Sanger’s Reagent (DNFB)
• Fluoro‐2,4‐dinitrobenzene and dansyl c
hloride
• Phenylisothiocyanate (Edman degradati
on)
B

7. Characterization of proteins and peptides involves
three different processes:
Determining the amino acid
composition.
Involves finding out the amin
o acids that make up the pro
tein and their number.
Determining the amino acid sequence
Involves finding out the sequence of amino
acids of the proteins in their order.
Determining the molecular mass
of the protein.
1 2 3

8. Platform and Analytical Techniques

9. 02
01
Creative BioMart performs a number of
routine protein analyses throughout all
project stages to understand the
heterogeneity, impurity profile, and potency
of a recombinant protein destined for
pharmaceutical use. We have developed the
platforms and analytical methods to pursue a
comprehensive chemical, physical, and
conformational characterization.
Creative BioMart provide comprehensive, high-
quality detection methods for the characterization
and analysis of protein drugs. Whether it is
characterizing reference products at different stages,
evaluating different batches of products, or used for
comparative research on biosimilars or modified
biopharmaceuticals, our services can meet scientific
standards and international regulatory requirements.
Our services

10. Great flexibility. We focus on
small projects and provide all
the flexibility you need.
01
Single point of contact. Due to the large
number of services provided internally,
all communications are centralized.
02
03
Standard and customized solutions. If our
standard solutions do not meet your
requirements, our skilled technicians will be
able to develop specialized solutions.
04
Complete service portfolio. Many
other chemical, microbiological and
ancillary services are provided in
GMP certification, WHO
prequalification and FDA approved
laboratories.
Our advantages

11. Contact Us
Address: 45-1 Ramsey Road, Shirley, NY 11967,
USA
Email: contact@creative-biomart.com
creativebiomart.net

12. THANK YOU FOR WATCHING
THANKS
Creative Biomart