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Effect of Cassava starch coating
on quality of foods
Presented by:
Gurudatta khandke
PALB-3308
TYPES
IMPORTANCE
HISTORY
3
PROCESSING AND METHODS
CONCLUSION
INTRODUCTION
REFERENCES
Introduction
 Edible coating is a thin layer of edible material placed on food
or between the food components which can be eaten as a part
of the whole food product.
 It prevents moisture loss, selectively allows gasses like CO2
and O2, which are involved in the respiration.
 Starch is one of the edible material used as film coating
because of its good mechanical properties.
4
• Cassava is a potential starch material for edible
coating, it is isotropic, odourless, tasteless, colourless
and flexible.
• Edible coatings are good as barriers to protect the
food from adverse biological, physical and chemical
changes.
5
History
 Bee Wax was the first edible coating .
 The Chinese used it for oranges and
lemons in 12th and 13th centuries.
 In England fats were used to prolong
shelf life of meat products called
larding.
 In mid 20th century, it is used to
prevent water loss and add glossiness to
fruits and vegetables.
Hyun et al, 1999
6
Important properties of edible coatings
 During storage, it should not
ferment, coagulate, separate, develop off flavour or spoil.
 It should be spread evenly, dry quickly and be easy to remove
from equipment.
 Once applied, it should not
crack, discolour, or peel during handling and storage.
 It should not adhere to packaging, react adversely with the
food.
 It should permit enough gas exchange to prevent from going
anaerobic and restrict to retard ripening.
 Coating should be a barrier to moisture to prevent sogginess.
7
Philips et al, 2004
Benefits of edible coatings
Improves -Appearance
-Structural properties
 Reduces -Water loss
-Gas diffusion
- Mold growth
- Uptake of frying oil
8
9
Components of edible
coatings
Polysaccharide Proteins Lipids Composite
Cellulose
Starches
Pectin
Sea weed extracts
Chitosan
Microbial
polysaccharides
Gelatin
Zein
Wheat gluten
Casein
Whey protein
Albumin
Soya protein
Waxes
•Bees wax
•Carnauba wax
•Paraffin wax
surfuctants
Philips et al, 2004
Blend of
Polysaccharide,
protein
or lipid
Polysaccharides
• Polysaccharides are hydrophilic in nature and intermediate
among coating materials in gas exchange properties but they
are poor barrier to moisture.
• Polysaccharide gums are hydrocolloids of considerable
molecular weight, and are water-soluble.
• Because of the size and configuration of their molecules, these
polysaccharide have the ability to thicken and/or gel aqueous
solutions as a result of both hydrogen bonding between
polymer chains and intermolecular friction when subjected to
shear.
10
Proteins : These are similar to
polysaccharides, also hydrophilic.
Lipids: Lipid and waxes are more permeable
to gasses but better barrier to water vapour.
Composites : Are blends of polysaccharide,
proteins and lipid.
• These are having combine effects on coated
foods.
11
Starches
• It is the most abundant plant product and it constitutes the
polysaccharide .
• It is the mixture of amylose and amylopectin.
• Amylose form films and coatings due its predominant linear
structure.
• Water acts as a plasticizer in the formation of films.
• Water decrease the strength of the film .
12
Philips et al, 2004
13
Gelatinization:
Increase volume,
viscosity, and
translucency of
starch when they are
heated in a liquid
Dextrinization:
If starch is subjected
to dry heat it
produces dextrins.
This break down
process is called
dextrinization.
Retrogradation
This a reaction that
takes place in
gelatinized starch
when the amylose
and amylopectin
bonds realign
themselves, causing
the liquid to form
gel.
Starch Characteristics
Changes in starch structure at different temperatures
Tongdeesoontorn et al., 2009 14
Tongdeesoontorn et al., 2009
15
Tuber Crop starch-properties
Tubers Starch % Viscosity Clarity Stability
Cassava 25-35 High High Medium
Sweet
potato
20-25 Medium-high High Medium
Yams 15-33 Medium-high High High
Aroids 10-20 Low-medium Low High
Canna 15-25 High High High
Arrowroot 16-28 Medium-high Medium Medium
16
Gbadeg et al., 2013
Starches available in market
17
349Rs /500gm
280Rs /Kg
1620Rs/500gm143Rs /500gm
180Rs/450gm
 Cassava is a shrub of the Euphorbiaceae (spurge) family.
 Native- South America.
 It is the third most important source of calories after rice and maize.
 In Africa, it is traditionally grown
as a staple food crop by small- scale
subsistence farmers in marginal areas.
 It is most drought-tolerant crops.
 It is a starch made by leaching and
drying the root of cassava plant. It is also called as
tapioca starch.
18
Cassava
Gbadegesin et al., 2013
Nomenclature
Kingdom: Plantae
Order: Malpighiales.
Family: Euphorbiaceae
Sub family: Crotonoideae
Genus: Manihot
Species : M. esculenta
Binomial name: Manihot esculenta.
19
Africa: (English) – cassava
Africa: (French) – manioc, maniocs
Africa: (Swahili) – mogo, mihogo
Brazil: mandioca, aipim, macaxeira
China: mushu
Ghana: bankye
Haiti: kassar
Holland: cassave
India: sagudana, sabudana, kappa
Different names of cassava
20
Philips et al, 2004
World scenario of cassava production
Global cassava development statergy, 2004
21
22
Processing of starch
Da et al., 2008
Cassava processing machine in a small scale
industry
Da et al., 2008
23
Cylindrical rasper
Da et al., 2008
24
Vertical stirring tank reactor
Cont........
Preparation of cassava starch
solution
• CS suspension were prepared at 70º C with
constant stirring and then cooled to room
temperature.
• Then the food to be coated is dipped in the
solution.
• Then the food is allowed to dry at room
temperature.
• The packaging of coated food is done.
25
Mechanism
• The principle of this technique is based on the creation of a
modified atmosphere around fruit surface, which could
preserve fruit quality characteristics.
• The ability of water soluble polysaccharides to reduce O2 and
increase CO2 levels in internal atmospheres of coated fruits
and vegetables reduces respiration rates
• It extend the shelf-life of fresh produce in a manner similar to
modified/controlled atmosphere storage.
26
Philips et al., 2004
1. Dipping. 2. Dripping.
.
3. Fluidized bed
coating.
.
Methods of coating edible material
27
4. Pannin 5.Spraying
.
28
Philips et al., 2004
Cont.........
Successful coating of edible starch
depends on
Types of coating material
Its specific formulation
The method of application
Surface characteristics of food
29
Cassava
starch
Food industry Textile industry
Adhesive
industry
Packaging
industry
30
Application of cassava starch
Excellent thickening
characteristics
Neutral taste
Desirable textural
characteristics
Cheap source
Easy to extract
Advantages
of cassava
starch
Philips et al., 200431
Selection of an edible starch coating for minimally
processed strawberry
To study the effect of cassava starch edible coatings with or
without potassium sorbate on quality attributes of minimally
processed strawberries.
32
Garcia et al., (2009) Food
Bioprocess Technol
11-10-2014
Objective
Methodology
According to CS concentration on the coating 1%, 2%, 3% the residual
solution was allowed to drip off for 1h, 2h and 3h respectively.
Fruits were packed PP and wrapped in PVC stretch films.
Stored at 5ºC until evaluation, after 24h of treatment.
Fruit coating
Fruits were washed in an 80- ppm of peracetic acid for 3 min.
Fruits were divided into 10 groups .
Nine groups were assigned to one of the nine coating treatment.
Edible coating solution
Prepared using aqueous solutions containing 1%, 2% or 3% of CS and 0%,
0.05% and 0.1% of potassium sorbate.
Solutions were heated to 70º C and cooled to room temperature.
3311-10-2014
34
1- 1% CS
2- 1% CS + 0.05% PS
3- 1% CS +0.1% PS
4- 2% CS
5- 2% CS+0.05% PS
6- 2% CS+0.1% PS
7- 3% CS
8- 3% CS+ 0.05% PS
9- 3% CS+0.1% PS
10- Control
 Surface colour, Sensory acceptance, Respiration rate and
water vapour resistance were measured.
11-10-2014
Table 1: Changes in colour of strawberry after treatment
L*- lightness, CS- Cassava starch, PS- potassium sorbate
Results
11-10-2014 35
Fig 1. Fruits coated with edible coatings
a- control sample. b- 3%CS edible coating, c- 3% CS+ 0.1%PS
11-10-2014 36
Table 2: Scores of sensory evaluation
CS- Cassava starch, PS- potassium sorbate
11-10-2014 37
Fig 2: Respiration rate test
a- control sample, b- sample coated with CS added with diff con of PS11-10-2014 38
Conclusion
39
Fig.3: Water vapour resistance test
Cassava starch edible coatings, with
or without potassium sorbate, did not
cause changes in strawberries’
superficial colour and showed good
sensory acceptance. Moreover, the
coatings were able to decrease the
respiration rate and increase water
vapour resistance of the samples
11-10-2014
Starch edible coating of papaya: effect on
sensory characteristics
To investigate the effects of coating of cassava starch and
carboxymethyl starch on sensory characteristics of papaya
during storage.
Castricini et al., 2012
Journal of food science
and technology
Objective
11-10-2014
40
Methodology
• Cassava formulations (1%, 3% and
5%) were obtained by heating CS in
distilled water at 70ºC with
continuous stirring and allowed for
cooling at room temperature.
CS
formulations
• CMS formulations (1%, 3% and 5%)
were obtained by dispersing
carboxymethyl starch in distilled
water with constant stirring at room
temperature.
CMS
formulations
Preparation of starch formulations
11-10-2014 41
The sensory evaluation of the fruits subjected to
different coatings comparing with reference sample were
done by nine selected and trained panel members.
The coated samples were stored at 20º C for 14 days.
5 fruits for each 6 treatments were coated by dipping.
7th treatment is control (without coating).
11-10-2014 42
Fig 1: Weight loss in fruit after treatment
CS- cassava starch, CS+CL- cassava starch + carboxymethyl.
11-10-2014 43
Results
Fig 2: Juice leakage in fruit after treatment
CS- cassava starch, CS+CL- cassava starch + carboxymethyl.
11-10-2014 44
Fig 3: Vit C content of fruit after treatment
Conclusion
11-10-2014
45
The cassava starch edible coating, with
and without the carboxymethyl, was
able to reduce weight loss and juice
leakage .However, the application of
the coating significantly reduced the
vitamin C content and this reduction
was more significant in sample pre-
treated with calcium.
Conclusion
• Cassava starch application is cheap, safe ,
tasteless, odourless, colourless, biodegradable,
non toxic.
• It increases the shelf life by decreasing
respiration rate.
• Reduces weight loss of foods by decreasing
juice leakage.
• Improves appearance of the food by adding
glossiness to the food.
46
Future front of line
• Edible food wraps that enhance nutrition for
food product could make healthy foods more
attractive.
• Edible coating could provide new flavour
combinations
47
References
• CHIUMARELLIA, M. AND HUBINGERA, D. M., 2011, Selection of
cassava starch – carnauba wax composite edible coating used to preserve
fresh-cut apples. Food colloid. 28 (1): p 59–67.
• DA, G., DUFOUR, D., MAROUZE, C., THANH, L. M. AND
MARCHAL, A. P., 2008, Cassava starch processing at small scale in
• North vietnam. Starch/stärke 60: 358–372
• GARCIA, C. L., PEREIRA, M. L. AND SARANTOPOULOS, L. D.,
2010, Selection of an edible starch coating for minimally processed
Strawberry. Food Bioprocess Technol. (3): 834-842.
48
• GBADEG ESIN, A. M., CHARLES. O., OLAIYA. AND BEECHING, R. J., 2013,
African cassava: biotechnology and molecular breeding to the rescue. British
Biotechnology Journal. 3(3): 305-317.
• LEVIC, B. L., KOPRIVICA, B. G., MISLJENOVIC, M. N., FILIPCEV, V. B. AND
KULJANIN, A. T., 2008, Effect of starch as an edible material on the process of
osmotic dehydration of carrot in saccharose solution and sugar beet molasses. Food
science and technology. 39: 29-36.
• PHILIPS, P. T. AND TAYLOR, S. D. (2004). The global cassava development
strategy. Newyork: Food and agriculture organization of the united nations.
• TONGDEESOONTORN, W., MAUER, J. L., WONGRUONG, S. AND
RACNCHAI, R., 2009, water vapour permiability and sorption isotherms of
cassava starch based films blended with gelatin and carboxymethyl cellulose. Asian
journal of food and agro-industry. (40), 501-514.
49
Contd....
50

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  • 1. 1
  • 2. 2 Effect of Cassava starch coating on quality of foods Presented by: Gurudatta khandke PALB-3308
  • 4. Introduction  Edible coating is a thin layer of edible material placed on food or between the food components which can be eaten as a part of the whole food product.  It prevents moisture loss, selectively allows gasses like CO2 and O2, which are involved in the respiration.  Starch is one of the edible material used as film coating because of its good mechanical properties. 4
  • 5. • Cassava is a potential starch material for edible coating, it is isotropic, odourless, tasteless, colourless and flexible. • Edible coatings are good as barriers to protect the food from adverse biological, physical and chemical changes. 5
  • 6. History  Bee Wax was the first edible coating .  The Chinese used it for oranges and lemons in 12th and 13th centuries.  In England fats were used to prolong shelf life of meat products called larding.  In mid 20th century, it is used to prevent water loss and add glossiness to fruits and vegetables. Hyun et al, 1999 6
  • 7. Important properties of edible coatings  During storage, it should not ferment, coagulate, separate, develop off flavour or spoil.  It should be spread evenly, dry quickly and be easy to remove from equipment.  Once applied, it should not crack, discolour, or peel during handling and storage.  It should not adhere to packaging, react adversely with the food.  It should permit enough gas exchange to prevent from going anaerobic and restrict to retard ripening.  Coating should be a barrier to moisture to prevent sogginess. 7 Philips et al, 2004
  • 8. Benefits of edible coatings Improves -Appearance -Structural properties  Reduces -Water loss -Gas diffusion - Mold growth - Uptake of frying oil 8
  • 9. 9 Components of edible coatings Polysaccharide Proteins Lipids Composite Cellulose Starches Pectin Sea weed extracts Chitosan Microbial polysaccharides Gelatin Zein Wheat gluten Casein Whey protein Albumin Soya protein Waxes •Bees wax •Carnauba wax •Paraffin wax surfuctants Philips et al, 2004 Blend of Polysaccharide, protein or lipid
  • 10. Polysaccharides • Polysaccharides are hydrophilic in nature and intermediate among coating materials in gas exchange properties but they are poor barrier to moisture. • Polysaccharide gums are hydrocolloids of considerable molecular weight, and are water-soluble. • Because of the size and configuration of their molecules, these polysaccharide have the ability to thicken and/or gel aqueous solutions as a result of both hydrogen bonding between polymer chains and intermolecular friction when subjected to shear. 10
  • 11. Proteins : These are similar to polysaccharides, also hydrophilic. Lipids: Lipid and waxes are more permeable to gasses but better barrier to water vapour. Composites : Are blends of polysaccharide, proteins and lipid. • These are having combine effects on coated foods. 11
  • 12. Starches • It is the most abundant plant product and it constitutes the polysaccharide . • It is the mixture of amylose and amylopectin. • Amylose form films and coatings due its predominant linear structure. • Water acts as a plasticizer in the formation of films. • Water decrease the strength of the film . 12 Philips et al, 2004
  • 13. 13 Gelatinization: Increase volume, viscosity, and translucency of starch when they are heated in a liquid Dextrinization: If starch is subjected to dry heat it produces dextrins. This break down process is called dextrinization. Retrogradation This a reaction that takes place in gelatinized starch when the amylose and amylopectin bonds realign themselves, causing the liquid to form gel. Starch Characteristics
  • 14. Changes in starch structure at different temperatures Tongdeesoontorn et al., 2009 14
  • 16. Tuber Crop starch-properties Tubers Starch % Viscosity Clarity Stability Cassava 25-35 High High Medium Sweet potato 20-25 Medium-high High Medium Yams 15-33 Medium-high High High Aroids 10-20 Low-medium Low High Canna 15-25 High High High Arrowroot 16-28 Medium-high Medium Medium 16 Gbadeg et al., 2013
  • 17. Starches available in market 17 349Rs /500gm 280Rs /Kg 1620Rs/500gm143Rs /500gm 180Rs/450gm
  • 18.  Cassava is a shrub of the Euphorbiaceae (spurge) family.  Native- South America.  It is the third most important source of calories after rice and maize.  In Africa, it is traditionally grown as a staple food crop by small- scale subsistence farmers in marginal areas.  It is most drought-tolerant crops.  It is a starch made by leaching and drying the root of cassava plant. It is also called as tapioca starch. 18 Cassava Gbadegesin et al., 2013
  • 19. Nomenclature Kingdom: Plantae Order: Malpighiales. Family: Euphorbiaceae Sub family: Crotonoideae Genus: Manihot Species : M. esculenta Binomial name: Manihot esculenta. 19
  • 20. Africa: (English) – cassava Africa: (French) – manioc, maniocs Africa: (Swahili) – mogo, mihogo Brazil: mandioca, aipim, macaxeira China: mushu Ghana: bankye Haiti: kassar Holland: cassave India: sagudana, sabudana, kappa Different names of cassava 20 Philips et al, 2004
  • 21. World scenario of cassava production Global cassava development statergy, 2004 21
  • 23. Cassava processing machine in a small scale industry Da et al., 2008 23 Cylindrical rasper
  • 24. Da et al., 2008 24 Vertical stirring tank reactor Cont........
  • 25. Preparation of cassava starch solution • CS suspension were prepared at 70º C with constant stirring and then cooled to room temperature. • Then the food to be coated is dipped in the solution. • Then the food is allowed to dry at room temperature. • The packaging of coated food is done. 25
  • 26. Mechanism • The principle of this technique is based on the creation of a modified atmosphere around fruit surface, which could preserve fruit quality characteristics. • The ability of water soluble polysaccharides to reduce O2 and increase CO2 levels in internal atmospheres of coated fruits and vegetables reduces respiration rates • It extend the shelf-life of fresh produce in a manner similar to modified/controlled atmosphere storage. 26 Philips et al., 2004
  • 27. 1. Dipping. 2. Dripping. . 3. Fluidized bed coating. . Methods of coating edible material 27
  • 28. 4. Pannin 5.Spraying . 28 Philips et al., 2004 Cont.........
  • 29. Successful coating of edible starch depends on Types of coating material Its specific formulation The method of application Surface characteristics of food 29
  • 30. Cassava starch Food industry Textile industry Adhesive industry Packaging industry 30 Application of cassava starch
  • 31. Excellent thickening characteristics Neutral taste Desirable textural characteristics Cheap source Easy to extract Advantages of cassava starch Philips et al., 200431
  • 32. Selection of an edible starch coating for minimally processed strawberry To study the effect of cassava starch edible coatings with or without potassium sorbate on quality attributes of minimally processed strawberries. 32 Garcia et al., (2009) Food Bioprocess Technol 11-10-2014 Objective
  • 33. Methodology According to CS concentration on the coating 1%, 2%, 3% the residual solution was allowed to drip off for 1h, 2h and 3h respectively. Fruits were packed PP and wrapped in PVC stretch films. Stored at 5ºC until evaluation, after 24h of treatment. Fruit coating Fruits were washed in an 80- ppm of peracetic acid for 3 min. Fruits were divided into 10 groups . Nine groups were assigned to one of the nine coating treatment. Edible coating solution Prepared using aqueous solutions containing 1%, 2% or 3% of CS and 0%, 0.05% and 0.1% of potassium sorbate. Solutions were heated to 70º C and cooled to room temperature. 3311-10-2014
  • 34. 34 1- 1% CS 2- 1% CS + 0.05% PS 3- 1% CS +0.1% PS 4- 2% CS 5- 2% CS+0.05% PS 6- 2% CS+0.1% PS 7- 3% CS 8- 3% CS+ 0.05% PS 9- 3% CS+0.1% PS 10- Control  Surface colour, Sensory acceptance, Respiration rate and water vapour resistance were measured. 11-10-2014
  • 35. Table 1: Changes in colour of strawberry after treatment L*- lightness, CS- Cassava starch, PS- potassium sorbate Results 11-10-2014 35
  • 36. Fig 1. Fruits coated with edible coatings a- control sample. b- 3%CS edible coating, c- 3% CS+ 0.1%PS 11-10-2014 36
  • 37. Table 2: Scores of sensory evaluation CS- Cassava starch, PS- potassium sorbate 11-10-2014 37
  • 38. Fig 2: Respiration rate test a- control sample, b- sample coated with CS added with diff con of PS11-10-2014 38
  • 39. Conclusion 39 Fig.3: Water vapour resistance test Cassava starch edible coatings, with or without potassium sorbate, did not cause changes in strawberries’ superficial colour and showed good sensory acceptance. Moreover, the coatings were able to decrease the respiration rate and increase water vapour resistance of the samples 11-10-2014
  • 40. Starch edible coating of papaya: effect on sensory characteristics To investigate the effects of coating of cassava starch and carboxymethyl starch on sensory characteristics of papaya during storage. Castricini et al., 2012 Journal of food science and technology Objective 11-10-2014 40
  • 41. Methodology • Cassava formulations (1%, 3% and 5%) were obtained by heating CS in distilled water at 70ºC with continuous stirring and allowed for cooling at room temperature. CS formulations • CMS formulations (1%, 3% and 5%) were obtained by dispersing carboxymethyl starch in distilled water with constant stirring at room temperature. CMS formulations Preparation of starch formulations 11-10-2014 41
  • 42. The sensory evaluation of the fruits subjected to different coatings comparing with reference sample were done by nine selected and trained panel members. The coated samples were stored at 20º C for 14 days. 5 fruits for each 6 treatments were coated by dipping. 7th treatment is control (without coating). 11-10-2014 42
  • 43. Fig 1: Weight loss in fruit after treatment CS- cassava starch, CS+CL- cassava starch + carboxymethyl. 11-10-2014 43 Results
  • 44. Fig 2: Juice leakage in fruit after treatment CS- cassava starch, CS+CL- cassava starch + carboxymethyl. 11-10-2014 44
  • 45. Fig 3: Vit C content of fruit after treatment Conclusion 11-10-2014 45 The cassava starch edible coating, with and without the carboxymethyl, was able to reduce weight loss and juice leakage .However, the application of the coating significantly reduced the vitamin C content and this reduction was more significant in sample pre- treated with calcium.
  • 46. Conclusion • Cassava starch application is cheap, safe , tasteless, odourless, colourless, biodegradable, non toxic. • It increases the shelf life by decreasing respiration rate. • Reduces weight loss of foods by decreasing juice leakage. • Improves appearance of the food by adding glossiness to the food. 46
  • 47. Future front of line • Edible food wraps that enhance nutrition for food product could make healthy foods more attractive. • Edible coating could provide new flavour combinations 47
  • 48. References • CHIUMARELLIA, M. AND HUBINGERA, D. M., 2011, Selection of cassava starch – carnauba wax composite edible coating used to preserve fresh-cut apples. Food colloid. 28 (1): p 59–67. • DA, G., DUFOUR, D., MAROUZE, C., THANH, L. M. AND MARCHAL, A. P., 2008, Cassava starch processing at small scale in • North vietnam. Starch/stärke 60: 358–372 • GARCIA, C. L., PEREIRA, M. L. AND SARANTOPOULOS, L. D., 2010, Selection of an edible starch coating for minimally processed Strawberry. Food Bioprocess Technol. (3): 834-842. 48
  • 49. • GBADEG ESIN, A. M., CHARLES. O., OLAIYA. AND BEECHING, R. J., 2013, African cassava: biotechnology and molecular breeding to the rescue. British Biotechnology Journal. 3(3): 305-317. • LEVIC, B. L., KOPRIVICA, B. G., MISLJENOVIC, M. N., FILIPCEV, V. B. AND KULJANIN, A. T., 2008, Effect of starch as an edible material on the process of osmotic dehydration of carrot in saccharose solution and sugar beet molasses. Food science and technology. 39: 29-36. • PHILIPS, P. T. AND TAYLOR, S. D. (2004). The global cassava development strategy. Newyork: Food and agriculture organization of the united nations. • TONGDEESOONTORN, W., MAUER, J. L., WONGRUONG, S. AND RACNCHAI, R., 2009, water vapour permiability and sorption isotherms of cassava starch based films blended with gelatin and carboxymethyl cellulose. Asian journal of food and agro-industry. (40), 501-514. 49 Contd....
  • 50. 50

Editor's Notes

  1. Animated Venn diagram (Intermediate) To reproduce the SmartArt effects on this slide, do the following: On the Home tab, in the Slides group, click Layout, and then click Blank. On the Insert tab, in the Illustrations group, click SmartArt. In the Choose a SmartArt Graphic dialog box, in the left pane, click Relationship. In the Relationship pane, click Stacked Venn, and then click OK to insert the graphic into the slide. Select the graphic, and then click one of the arrows on the left border. In the Type your text here dialog box, enter text. Select the graphic. On the Design tab, in the Themes group, click Colors, and then under Built-in click Median. (Note: if this action is taken in a PowerPoint presentation containing more than one slide, the theme color will be applied to all of the slides.) Under SmartArt Tools, on the Design tab, in the SmartArt Styles group, do the following: Click Change Colors, and then under Colorful click Colorful – Accent Colors (first option from the left). Click More, and then under 3-D click Polished (first row, first option from the left). On the Home tab, in the Font group, select Franklin Gothic Medium Cond from the Font list, and then select 28 from the Font Size list. To reproduce the animation effects on this slide, do the following: On the slide, select the graphic. On the Animations tab, in the Advanced Animation group, click Add Animation, under Entrance click Fly In. Also on the Animations tab, in the Timing group, in the Duration box, enter 1.00 seconds. Also on the Animations tab, in the Advanced Animation group, click Add Animation, and then under Entrance click Zoom. Also on the Animations tab, in the Timing group, in the Duration box, enter 1.00 seconds. Also on the Animations tab, in the Advanced Animation group, click Add Animation, and then under Emphasis click Grow/Shrink. Also on the Animations tab, in the Timing group, in the Duration box, enter 1.00 seconds. Also on the Animations tab, in the Animation group, click the Show Additional Effect Options dialog box launcher. In the Grow/Shrink dialog box, do the following: On the Effect tab, under Settings, in the Size list, in the Custom box, enter 30%, and then press ENTER. Also in the Size list, click Vertical. Also on the Effect tab, under Settings, select Auto-reverse. On the SmartArt Animation tab, in the Group graphic list, select One by One. On the Animations tab, in the Advanced Animation group, click Animation Pane. In the Animation Pane, click the double arrow under each animation effect to expand the contents of the list of effects. Also in the Animation Pane, select the first animation effect (first fly-in effect). On the Animations tab, in the Timing group, in the Start list, select With Previous. Also on the Animations tab, in the Animation group, click Effect Options, and then under Direction click From Bottom-Left. In the Animation Pane, select the second animation effect (second fly-in effect). On the Animations tab, in the Animation group, click Effect Options, and then under Direction click From Bottom-Right. In the Animation Pane, select the third animation effect (third fly-in effect). On the Animations tab, in the Animation group, click Effect Options, and then under Direction click From Bottom-Left. In the Animation Pane, select the fourth animation effect (fourth fly-in effect). On the Animations tab, in the Animation group, click Effect Options, and then under Direction click From Bottom-Right. In the Animation Pane, press and hold CTRL, select the fifth, sixth, seventh, eighth, ninth, 10th, 11th, and 12th animation effects (all of the faded zoom effects and grow/shrink effects). On the Animations tab, in the Timing group, in the Start list, select With Previous. Also in the Animation Pane, do the following to reorder the list of effects: Drag the fifth animation effect (first faded zoom effect) until it is second in the list of effects. Drag the sixth animation effect (second faded zoom effect) until it is fourth in the list of effects. Drag the seventh animation effect (third faded zoom effect) until it is sixth in the list of effects. Drag the ninth animation effect (first grow/shrink effect) until it is third in the list of effects. Drag the 10th animation effect (second grow/shrink effect) until it is sixth in the list of effects. Drag the 11th animation effect (third grow/shrink effect) until it is ninth in the list of effects. To reproduce the background effects on this slide, do the following: Right-click the slide background area, and then click Format Background. In the Format Background dialog box, click Fill in the left pane, select Gradient fill in the right pane, and then do the following: In the Type list, select Radial. In the Direction, list click From Corner (first row, second option from the left) Under Gradient stops, click Add gradient stop or Remove gradient stop until two stops appear in the slider. Also under Gradient stops, customize the gradient stops as follows: Select the first stop in the slider, and then do the following: In the Position box, enter 0%. Click the button next to Color, and then under Theme Colors click White, Background 1 (first row, first option from the left). Select the last stop in the slider, and then do the following: In the Position box, enter 100%. Click the button next to Color, and then under Theme Colors click White, Background 1, Darker 35% (fifth row, first option from the left).