PROTIEN CRYSTAL bioltechnology london

1. PROTIEN
CRYSTAL
ARSHAD WAHAB
170151601018
IIIrd year B.tech Biotech

2. Proteincrystalsareusefulinthestudy
ofproteinstructuresforuseinmedicine,amongst
otherapplications.
Intheprocessofproteincrystallization,proteinsare
dissolvedinanaqueousenvironmentandsample
solutionuntiltheyreachthesupersaturatedstate.
WHAT IS
PROTIEN
CRYSTAL ?
Protein crystal formations of lysozyme
20/01/2020 Creator:- ARSHAD WAHAB (170151601018) 3RD .YEAR B.TECH BIOTECH. BSARCIST
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3. MACROMOLECULES
IN
CRYSTALLINESTATE
This is usually achieved by carrying out an extensive series, or establishing a
vast matrix, of crystallization trials, evaluating the results and using the
information that is obtained to improve conditions in successive rounds of
trials.
Macromolecular crystallization is generally a matter of searching, as
systematically as possible, the ranges of the individual parameters that influence
1. crystal formation. 2. finding a set .
3. multiple sets of factors that yield
some kind of crystals.
4. optimizing the individual variables
to obtain the best possible crystals.
PARAMETRS.
20/01/2020 Creator:- ARSHAD WAHAB (170151601018) 3RD .YEAR BTSCH BIOTECH. BSARCIST
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4. Crystallizationofamacromoleculeabsolutelyrequiresthecreationofa
supersaturatedstate.Thisisillustratedbythephasediagramforcrystal
growth.CRYSTALLIZATION
OF
MACROMOLECULES
The phase diagram for the crystallization of
macromolecules. The solubility diagram is
divided sharply into a region of
undersaturation and a region of
supersaturation by the line denoting maximum
solubility at specific concentrations of a
precipitant, which may be salt or a polymer.
The region of supersaturation is further
divided into the metastable and labile
regions.
• In the metastable region nuclei will develop into
crystals, but no nucleation will occur.
• In the labile region both might be expected to
occur.
• The final region, at very high supersaturation, is
denoted the precipitation region, where this
result might be most probable.
Crystals can only be grown from a
supersaturated solution, and creating
such a solution supersaturated in the
protein of interest is the immediate
objective in growing protein crystals.
20/01/2020 Creator:- ARSHAD WAHAB (170151601018) 3RD .YEAR BTSCH BIOTECH. BSARCIST
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5. Why to determine the
3D strucures?
Biological structures are too complex
to understand.
Structural understanding is very less.
Xray wouldn’t help as it will scatter in
random directions.
So what’s the solution ?
WHY DOWE
NEEDTO
GROW
CRYSTAL?
So after aligning the structure of protein such as of in crystal.
Then after passing xray through crystal will give us a steady information
to let us justify the 3D dimensional structure of protein very easily .
20/01/2020 Creator:- ARSHAD WAHAB (170151601018) 3RD .YEAR BTECH BIOTECH. BSARCIST
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6. Robotic approaches to deploying multiple
crystallization condition matrices has required the
design and fabrication of new plasticware and plates
Robotics and
automation of
crystallization
Robotic systems are efficient, tireless and accurate, but in
addition, they offer another important feature.They can
carry out experiments using drop samples of very small
volume.
‘High-throughput’ facilities currently use magnification optics and
cameras to robotically and periodically examine individual
samples and present images in some form to reviewers.
20/01/2020 Creator:- ARSHAD WAHAB (170151601018) 3RD .YEAR BTECH BIOTECH. BSARCIST
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7. Diamond Light Source (or Diamond) is the UK's
national synchrotron light source science facility located at
the Harwell Science and Innovation Campus in Oxfordshire.
Its purpose is to produce intense beams of light whose
special characteristics are useful in many areas of scientific
research. In particular it can be used to investigate the
structure and properties of a wide range of materials
from proteins.
Diamond generates synchrotron light at wavelengths
ranging from X-rays to the far infrared.This is also known
as synchrotron radiation and is the electromagnetic
radiation emitted by charged particles travelling near
the speed of light. It is used in a huge variety of experiments
to study the structure and behaviour of many different
types of matter.
DIAMOND stands for :- DIpole And Multipole Output for
the Nation at Daresbury. (location being changed ).
WHERE DOES
PROTIEN CRYSTAL
GET DETERMINED ?
20/01/2020 Creator:- ARSHAD WAHAB (170151601018) 3RD .YEAR BTECH BIOTECH. BSARCIST
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8. ProteinX-raycrystallographyisatechniqueusedtoobtain
thethree-dimensionalstructureofaparticularproteinbyx-
raydiffractionofitscrystallizedform....Makingcrystals
createsalatticeinwhichthistechniquealignsmillions
ofproteinsmoleculestogethertomakethedatacollection
moresensitive.
X-ray crystallography is a tool used for determining the
atomic and molecular structure of a crystal.The underlying
principle is that the crystalline atoms cause a beam of X-
rays to diffract into many specific directions
PROCESS OF
IDENTIFYINGTHE
STRUCTURES OF
PROTIEN CRYSTAL
20/01/2020 Creator:- ARSHAD WAHAB (170151601018) 3RD .YEAR BTECH BIOTECH. BSARCIST
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9. HELP
ANY QUESTIONS REAGARDING ANYTHING PLEASE FEEL FREETO CONTACT
TO CREATOR OF SLIDE.
OUT COMES OF
PROTIEN CRYSTAL
STRUCTURE
DETERMINATION.
WHAT ARETHE APPLICATIONS OF
THIS CRYSTAL STRUCTURE
DETERMINATION?
Biological molecule
of your body interact
with one another.
Design a drug that
will bind more
efficiently.
Design a protein that
nature would never
made it.
(Artificial Digoxigenin
binding protein)
20/01/2020 Creator:- ARSHAD WAHAB (170151601018) 3RD .YEAR BTECH BIOTECH. BSARCIST
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10. 20/01/2020 Creator:- ARSHAD WAHAB (170151601018) 3RD .YEAR B.TECH BIOTECH. BSARCIST
10
SPECIAL
THANKS SLIDE SHAREWAS PREPARED UNDERTHE GUIDANCE OF
SHAZIA MAM
(ASSITANT PROFFESSOR)
SCHOOL OF LIFE SCIENCE.
B.S. ABDUR RAHMAN CRESCENT INSTITUTE OF SCIENCE
ANDTECHNOLOGY

11. CREDITS.
THE ROYAL
INSTITUION.
THE NATIONAL
CENTRE FOR
BIOTECHNOLOGY
INFORMATION.
DIAMOND LIGHT
SOURCE.
Professor Elspeth Garman take us on a
journey into the world of crystallography -
from protein production and purification
to growing the right type of crystals.
Professor Stephen Curry takes us on a journey
into the Diamond Light Source. Professor Curry
explores the inner workings of the Diamond
Light Source to reveal how such facilities are
aiding the field of structural biology and
continuing the work of the early crystallography
pioneers 100 years on.
Introduction to protein crystallization
Alexander McPherson and Jose A. Gavira
Published online 2013 Dec 24.
PMCID: PMC3943105 doi: 10.1107/S2053230X13033141
PMID: 24419610
Diamond Light Source, UK.
20/01/2020 Creator:- ARSHAD WAHAB (170151601018) 3RD .YEAR B.TECH BIOTECH. BSARCIST 11