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Exercise 2
Enzymes
Asuncion, Arnel
Dela Cruz, Ma. Beatriz
Noblado, Jomar
Santos, Christian
Introduction
Enzymes
-are proteins that accelerate a certain chemical
reactions by lowering the activation energy
Introduction
Enzymes
-are proteins that accelerate a certain chemical
reactions by lowering the activation energy
-Factors affecting enzyme activity
1. Temperature
2. pH
3. Enzyme and substrate concentration
4. Inhibitors
Introduction
Hydrolases
1.Amylase
Introduction
Hydrolases
1.Amylase
2.Sucrase
Oxidoreductase
1. Dehydrogenase
Introduction
Oxidoreductase
1. Dehydrogenase
2. Catalase
Introduction
Introduction
Lugol’s test
Benedict’s test
Methodology
A. Test for amylase
B. Test for sucrase
C. Test for dehydrogenase
D. Test for catalase
Results and Discussion
A. Test for amylase
Figure 1. Test for amylase. Left: With corn, Right: Control
B. Test for sucrase
Results and Discussion
Figure 2. Test for sucrase. Left: With corn, Right: Control
C. Test for dehydrogenase
Results and Discussion
Figure 3. Test for dehydrogenase. Left: control, Right: with
germinated mung beans
C. Test for catalase
Results and Discussion
Figure 4. Test for catalase. Left: fresh potato strips, Right:
boiled potato strips
Conclusion
1. Roots of newly germinated seeds of Zea
mays were able to hydrolyze starch and
sucrose.
2. Methylene blue was reduced by germinated
Vigna radiata.
3. An evolution of gas can be observed in a
fresh potato but it cannot be seen in a boiled
potato.

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