1. CCAMBIO and the mARS
project
Anton Van de Putte
CCAMBIO Annual Meeting
12 may 2014
2. Microbial Antarctic
Resources System
An information system dedicated to facilitate the
discovery, access and analysis of geo-
referenced,
molecular microbial diversity (meta)data generated
by Antarctic researchers, in an Open fashion.
3. What’s happened so far
• mARS Workshop hosted at the Belgian Science Policy
Office (BELSPO, Brussels) in May 2012
• mARS Workshop held during the SCAR Open
Science Conference (Portland, OR) in July 2012
• Technical mARS Workshop hosted at the Université
Libre de Bruxelles in December 2013
• Initiate the development of the database and
webplatform
4. Near future planning
• mARS Workshop held during the SCAR Open
Science Conference (Auckland, NZ) on 27 august
2014
• Present a proof of concept of the dataindrastructure to
be used for mARS
11. Getting Data into mARS
• Requires that
• Data is accessible in a public a public repository
(Genbank, IMG-M or other web accessible)
• 2 additional metadata files
• MiMARKS
• Microbial Sequence spreadsheet
12. 0. Before you start
• 1. Clearly Identify your needs
• You have a project that you would like to register
with mARS
• no sequence data or environmental data at this
point: skip Steps 1, 2, 4 and 7
• environmental data, but no publicly available
sequences yet, follow all Steps below, but do not
enter Sequence IDs in the forms.
• environmental data, and publicly available
sequences. Follow all Steps below.
13. 0. Before you start
• Send an email to request a username and password
from the IPT administrator
14.
15. 0. Before you start
• Send an email to request a username and password
from the IPT administrator
• Make a copy of the MiMarks Googlesheet from the
RDP MiMarks Googlesheet (click on “Make copy” from
the “File” menu).
16.
17. 0. Before you start
• Send an email to request a username and password
from the IPT administrator
• Make a copy of the MiMarks Googlesheet from the
RDP MiMarks Googlesheet (click on “Make copy” from
the “File” menu).
• Make a copy of the Microbial Sequence Set from the
mARS Googlesheet (click on “Make copy” from the
“File” menu).
18.
19. 1. Prepare your MiMarks
spreadsheet• In the MiMarks Googlesheet you’ve created in step 0,
fill in your environmental metadata details using the
“Google Documents” interface, following the
instructions available from the MiMarks Googlesheet
documentation at RDP. Example files are available
from the mARS website.
• In the header for each column that will hold your
sequence set data, list the unique identifier of your
sequence set.
• Once you are finished, download your spreadsheet as
a CSV (Comma-separated Values) file on your
computer.
20.
21. 2. Prepare your Microbial
Sequence Set spreadsheet
• In the Microbial Sequence Set Googlesheet you’ve
created in step 0, fill all the fields (replace the
examples available from the Googlesheet)
• Once you are finished, download your spreadsheet as
a CSV (Comma-separated Values) file on your
computer.
22.
23. 3. Describe your data in the
IPT
• Login the IPT using your credentials:
• Use the form at the bottom of the “Manage Resource”
page to create a new resource. Provide a unique
"shortname" for your dataset.
• Click the “Create” button. You will arrive on the
Resource Management page.
• Click on the “Edit” button in the Metadata section on
the left and fill in the details for the different metadata
sections. A detailed instructions are available from IPT
quick reference guide. Hint: mention your grant
number in the “Project Data” section, to allow us to link
your resource to relevant projects in the GCMD/AMD.
24. 4. Upload your MiMarks and
Microbial Sequence Set
• 1. In your IPT session, from your Resource
Management page, click on the “Choose file” button in
the “Source data” section on the left of the page.
• 2. Point to your completed MiMarks CSV, and click on
“Choose”
• 3. Click on the “add” button in the “Source data”
section on the left of the page then click on the “Save”
button on the bottom. Your MiMarks CSV file is now
uploaded on the IPT.
25. 4. Upload your MiMarks and
Microbial Sequence Set
• 5. From your Resource Management page, click on
the “Choose file” button in the “Source data” section on
the left of the page.
• 6. Point to your completed Microbial Sequence Set
CSV, and click on “Choose”
• 7. Click on the “add” button in the Source data section
on the left of the page, then click on the “Save” button.
Your Microbial Sequence Set CSV file is now uploaded
on the IPT.
26. 5. Publish and register your
data
• From your Resource Management page, click on the
“Publish” button in the “Published release” section on
the left of the page. Do not worry when you see a
warning message “Source data or Darwin Core
mappings missing. No data archive generated
• By default, your resource’s visibility is set to “Private”.
To allow your resource to become visible on the IPT
for all users, click on the “Public” button in the
“Visibility” section.
• Request one of the administrators to “Register” your
dataset.
Editor's Notes
This step will capture information about molecular microbial diversity research efforts that are being or have been conducted by the Antarctic research community. The results of step 1 will facilitate communication and collaboration, augment comparative biodiversity studies, and provide a legacy- discoverable resource to advance science, conservation awareness and management. The scope of the information that can be entered in the IPT encompasses present, past, or future studies involving marker gene surveys (e.g.16S or 18S rRNA, functional genes), or meta “omic” projects from natural samples in Antarctic habitats, enrichment or pure culture efforts
Secondly, users will be invited to upload habitat and molecular methods-specific (meta)data pertaining to the samples and the related sequencing data (including accession numbers) using standardized accessible on the mARS website. These templates can readily be shared with your collaborators and it works with GenBank submission tools (Sequin and WebIN).
Used together, and uploaded with the corresponding IPT metadata entry (as described in Step 1), these templates will describe geo-referenced physiochemical information that relates to Antarctic microbial diversity studies as well as the matching sequencing information.
In this step, sequence data files produced by different technologies (e.g. Sanger sequencing, 454, Illumina, Ion Torrent) will be linked back to the relevant entries as described in steps 1 and 2.
mARSwill provide indexed searching capabilities and geo-server links to DNA sequence data from Antarctic studies that have been deposited in public repositories, providing rapid access to this information through the biodiversity.aqdata portal.
There is currently no exhaustive resource that provides this level of information from a geo-referenced perspective. The Antarctic scientific community is actively engaged in molecular icrobial diversity and genomic surveys in both terrestrial and marine realms. mARSprovides a unique resource to harness this information.
As the primary mandate of biodiversity.aqis to provide the scientific community access to Antarctic diversity information, biodiversity.aq staff will process the microbial diversity information referenced in mARS for selected, highly used regions of marker genes (for each domain of life) generated through both Sanger sequencing studies and NGS efforts in order to provide the users with a window into the microbial diversity present in Antarctica.
This SOP details how you can upload (meta)data to mARS. To ensure this procedure only has to been carried out once, the mARS team has devoted special care to following widely-used standards for biodiversity data, as promoted by the Global Biodiversity Information Facility and the Genomics Standards Consortium. In this particular case, this SOP is built around two main types of standards, namely DarwinCore and MiMarks,ensuring maximal interoperability with internationally-recognized data and metadata repositories.
