SlideShare a Scribd company logo

Pre-Final Draft 3 Phosphate Prouct Sheet

1 of 2
Download to read offline
334 Hecla Street, Lake Linden, MI 49945
tech@nitrate.com | 1.906.296.1130
sales@nitrate.com | 1.906.296.1115
www.nitrate.com
Specificity:
Sensitivity:
Analysis Ranges:
Sample Types:
Storage Conditions:
Active Temperature Range:
Buffer Specifications:
Enzymatic Orthophosphate Test
NECi’s Enzymatic Orthophosphate Test is a highly specific, sensitive and
selective assay for the quantitative analysis of soluble reactive phosphate
(SRP) in many types of sample matrices.
Quantitative orthophosphate analysis is useful for evaluating soil health,
environmental health, industry process control, wastewater nutrient
levels, and much more.
This method is compatible with the persulfate digestion for total
phosphorus analysis. Total P kits based on the USGS method will be
available soon.
Utilizes a recombinant enzyme Purine nucleoside phosphorylase
(PNP) to catalyze the reaction of phosphate with substrate
2-amino-6-mercapto-7-methylpurine ribonucleoside (MESG). The
resulting 2-amino-6-mercapto-7-methylpurine (AMMP) absorbs
at 360 nm for accurate photometric analysis of phosphate.
Replaces the ascorbic acid method for more precise analysis.
Results in phosphorus units are obtainable with a simple
conversion factor.
Small reaction volume, minimal sample preparation, &
non-hazardous materials or waste.
Available in test tube, microplate, & on-site formats or
build your own assay with enzyme reagent packs.
Orthophosphate
15 - 40 °C
200 mM HEPES | 20 mM MgCl2
pH 7.6
Water
Soil
Aqueous Extracts
Low: 0.5 - 5.0 ppm
Standard: 5.0 - 20.0 ppm
0.5 ppm MDL
3+ Years @ -80°C
1+ Year @ -20°C
6 Months @ 4°C
334 Hecla Street, Lake Linden, MI 49945
tech@nitrate.com | 1.906.296.1130
sales@nitrate.com | 1.906.296.1115
A Closer Look at the Reaction
Overview of the Method
Example Standard Curve
H2PO4
-
2-amino-6-mercapto-7-methylpurine ribonucleoside
+
2-amino-6-mercapto-7-methylpurine
Purine Nucleoside
Phosphorylase
MESG
Ribose-1-phosphate
+
Wait
10 Min
900 µL
Reaction
Mixture
Combine Reaction Mixture
+ Sample into test tube
100 µL
Sample
Fill to
20.5 mL
with
Buffer
Prepare Stock
Reaction Mixture
1 mL
PNP
1 mL
MESG
By using a set of phosphate standards of known
concentration, a standard curve is used to
calibrate the method and quantify phosphate
content of unknown samples.
When used in conjunction with NECi’s Handheld
Photometer, results can be obtained on any
Android mobile device. The app software has a
pre-programmed standard curve and displays
data in ppm phosphate.
Measure A-360 of each sample
in UV compatible cuvettes
Note: ppm Phosphate x 0.3262 = ppm Phosphorus
Order online at nitrate.com/store/index.php/phosphate

Recommended

BIOshell_Poster-at-HPLC-2014_New-Orleans
BIOshell_Poster-at-HPLC-2014_New-OrleansBIOshell_Poster-at-HPLC-2014_New-Orleans
BIOshell_Poster-at-HPLC-2014_New-OrleansRoy Eksteen
 
New Ultra-High Capacity TOYOPEARL® Protein A Resin
New Ultra-High Capacity TOYOPEARL® Protein A Resin New Ultra-High Capacity TOYOPEARL® Protein A Resin
New Ultra-High Capacity TOYOPEARL® Protein A Resin Tosoh Bioscience GmbH
 
Tosoh Bioscience About Us 11c Linkedin
Tosoh Bioscience About Us 11c LinkedinTosoh Bioscience About Us 11c Linkedin
Tosoh Bioscience About Us 11c LinkedinRomain Dabre
 
Charity drive report
Charity drive reportCharity drive report
Charity drive reportHafiz Yeo
 
ฉันเหมือนใคร
ฉันเหมือนใครฉันเหมือนใคร
ฉันเหมือนใครAuilovely
 

More Related Content

Viewers also liked

Viewers also liked (10)

Authority model for_india
Authority model for_indiaAuthority model for_india
Authority model for_india
 
3 gppevolutionwp
3 gppevolutionwp3 gppevolutionwp
3 gppevolutionwp
 
CURSO DE TICS: COMPETENCIAS TRANSVERSALES EN LA ERA DIGITAL
CURSO DE TICS: COMPETENCIAS TRANSVERSALES EN LA ERA DIGITALCURSO DE TICS: COMPETENCIAS TRANSVERSALES EN LA ERA DIGITAL
CURSO DE TICS: COMPETENCIAS TRANSVERSALES EN LA ERA DIGITAL
 
Kopsavilkums
KopsavilkumsKopsavilkums
Kopsavilkums
 
8.15.16 FINAL Photometer Booklet - Half Letter Size
8.15.16 FINAL Photometer Booklet - Half Letter Size8.15.16 FINAL Photometer Booklet - Half Letter Size
8.15.16 FINAL Photometer Booklet - Half Letter Size
 
Excel vs fraud an analysis saran kumar u
Excel vs fraud an analysis   saran kumar uExcel vs fraud an analysis   saran kumar u
Excel vs fraud an analysis saran kumar u
 
Nuorisoasiainkeskus strategia
Nuorisoasiainkeskus strategiaNuorisoasiainkeskus strategia
Nuorisoasiainkeskus strategia
 
Neumonia pediatria
Neumonia pediatriaNeumonia pediatria
Neumonia pediatria
 
Indirect tax
Indirect taxIndirect tax
Indirect tax
 
Ратови грка
Ратови гркаРатови грка
Ратови грка
 

Similar to Pre-Final Draft 3 Phosphate Prouct Sheet

Fertilizer analysis
Fertilizer analysisFertilizer analysis
Fertilizer analysisAkma Ija
 
Water Analysis Using LAMBDA UV/Visible Spectrophotometers: Ortho-Phosphate De...
Water Analysis Using LAMBDA UV/Visible Spectrophotometers: Ortho-Phosphate De...Water Analysis Using LAMBDA UV/Visible Spectrophotometers: Ortho-Phosphate De...
Water Analysis Using LAMBDA UV/Visible Spectrophotometers: Ortho-Phosphate De...PerkinElmer, Inc.
 
plain 4 SYNTHESIS, PURIFICATION AND STABILITY STUDY OF ISLET
plain 4 SYNTHESIS, PURIFICATION AND STABILITY STUDY OF ISLETplain 4 SYNTHESIS, PURIFICATION AND STABILITY STUDY OF ISLET
plain 4 SYNTHESIS, PURIFICATION AND STABILITY STUDY OF ISLETAndrew Apals
 
P2 o5 in fertilizer
P2 o5 in fertilizerP2 o5 in fertilizer
P2 o5 in fertilizersinglethien
 
Purification and Characterization of Manganese Peroxidase of the White Rot Fu...
Purification and Characterization of Manganese Peroxidase of the White Rot Fu...Purification and Characterization of Manganese Peroxidase of the White Rot Fu...
Purification and Characterization of Manganese Peroxidase of the White Rot Fu...ssuser20fa83
 
Lecture 1 enzyme assays nov02 2007
Lecture 1 enzyme assays nov02 2007Lecture 1 enzyme assays nov02 2007
Lecture 1 enzyme assays nov02 2007mdroslan09
 
Phosphate based fertilizers
Phosphate based fertilizersPhosphate based fertilizers
Phosphate based fertilizersAdeel Hasnain
 
Sayantani Ghosh Dissertation
Sayantani Ghosh DissertationSayantani Ghosh Dissertation
Sayantani Ghosh DissertationSayantani Ghosh
 
Development and validation of stability indicating RP-HPLC method for estimat...
Development and validation of stability indicating RP-HPLC method for estimat...Development and validation of stability indicating RP-HPLC method for estimat...
Development and validation of stability indicating RP-HPLC method for estimat...SriramNagarajan18
 
Last Revision LT BioChem 20
Last Revision LT BioChem 20Last Revision LT BioChem 20
Last Revision LT BioChem 20Tony Rivera
 
Validation of an extraction technique based on tributyl phosphate
Validation of an extraction technique based on tributyl phosphateValidation of an extraction technique based on tributyl phosphate
Validation of an extraction technique based on tributyl phosphateNasir Othman
 
Enzyme Based Analytical Chemistry - Nitrate and the U.S. EPA
Enzyme Based Analytical Chemistry - Nitrate and the U.S. EPAEnzyme Based Analytical Chemistry - Nitrate and the U.S. EPA
Enzyme Based Analytical Chemistry - Nitrate and the U.S. EPAAnna-Marie Davidson
 
Efficient and Re-usable SAPO Catalyst for the Selective Production of Furans ...
Efficient and Re-usable SAPO Catalyst for the Selective Production of Furans ...Efficient and Re-usable SAPO Catalyst for the Selective Production of Furans ...
Efficient and Re-usable SAPO Catalyst for the Selective Production of Furans ...pbpbms6
 
A new RP-HPLC method development and validation of Sofosbuvir in bulk and pha...
A new RP-HPLC method development and validation of Sofosbuvir in bulk and pha...A new RP-HPLC method development and validation of Sofosbuvir in bulk and pha...
A new RP-HPLC method development and validation of Sofosbuvir in bulk and pha...SriramNagarajan17
 
JBEI Highlights - October 2014
JBEI Highlights - October 2014JBEI Highlights - October 2014
JBEI Highlights - October 2014Irina Silva
 
NOVEL SEPARATION AND QUANTITATIVE DETERMINATION OF LEVOFLOXACIN, PRULIFLOXACI...
NOVEL SEPARATION AND QUANTITATIVE DETERMINATION OF LEVOFLOXACIN, PRULIFLOXACI...NOVEL SEPARATION AND QUANTITATIVE DETERMINATION OF LEVOFLOXACIN, PRULIFLOXACI...
NOVEL SEPARATION AND QUANTITATIVE DETERMINATION OF LEVOFLOXACIN, PRULIFLOXACI...Dr. Ravi Sankar
 
Assessing The Impact Of Agricultural Practices On Phosphorous Availability An...
Assessing The Impact Of Agricultural Practices On Phosphorous Availability An...Assessing The Impact Of Agricultural Practices On Phosphorous Availability An...
Assessing The Impact Of Agricultural Practices On Phosphorous Availability An...National Institute of Food and Agriculture
 

Similar to Pre-Final Draft 3 Phosphate Prouct Sheet (20)

Fertilizer analysis
Fertilizer analysisFertilizer analysis
Fertilizer analysis
 
Water Analysis Using LAMBDA UV/Visible Spectrophotometers: Ortho-Phosphate De...
Water Analysis Using LAMBDA UV/Visible Spectrophotometers: Ortho-Phosphate De...Water Analysis Using LAMBDA UV/Visible Spectrophotometers: Ortho-Phosphate De...
Water Analysis Using LAMBDA UV/Visible Spectrophotometers: Ortho-Phosphate De...
 
Chromatography: Trends and Developments in MAb Screening and Characterization
Chromatography: Trends and Developments in MAb Screening and CharacterizationChromatography: Trends and Developments in MAb Screening and Characterization
Chromatography: Trends and Developments in MAb Screening and Characterization
 
plain 4 SYNTHESIS, PURIFICATION AND STABILITY STUDY OF ISLET
plain 4 SYNTHESIS, PURIFICATION AND STABILITY STUDY OF ISLETplain 4 SYNTHESIS, PURIFICATION AND STABILITY STUDY OF ISLET
plain 4 SYNTHESIS, PURIFICATION AND STABILITY STUDY OF ISLET
 
P2 o5 in fertilizer
P2 o5 in fertilizerP2 o5 in fertilizer
P2 o5 in fertilizer
 
Purification and Characterization of Manganese Peroxidase of the White Rot Fu...
Purification and Characterization of Manganese Peroxidase of the White Rot Fu...Purification and Characterization of Manganese Peroxidase of the White Rot Fu...
Purification and Characterization of Manganese Peroxidase of the White Rot Fu...
 
Nitrate
NitrateNitrate
Nitrate
 
Lecture 1 enzyme assays nov02 2007
Lecture 1 enzyme assays nov02 2007Lecture 1 enzyme assays nov02 2007
Lecture 1 enzyme assays nov02 2007
 
Phosphate based fertilizers
Phosphate based fertilizersPhosphate based fertilizers
Phosphate based fertilizers
 
Analytical toxicology
Analytical toxicologyAnalytical toxicology
Analytical toxicology
 
Sayantani Ghosh Dissertation
Sayantani Ghosh DissertationSayantani Ghosh Dissertation
Sayantani Ghosh Dissertation
 
Development and validation of stability indicating RP-HPLC method for estimat...
Development and validation of stability indicating RP-HPLC method for estimat...Development and validation of stability indicating RP-HPLC method for estimat...
Development and validation of stability indicating RP-HPLC method for estimat...
 
Last Revision LT BioChem 20
Last Revision LT BioChem 20Last Revision LT BioChem 20
Last Revision LT BioChem 20
 
Validation of an extraction technique based on tributyl phosphate
Validation of an extraction technique based on tributyl phosphateValidation of an extraction technique based on tributyl phosphate
Validation of an extraction technique based on tributyl phosphate
 
Enzyme Based Analytical Chemistry - Nitrate and the U.S. EPA
Enzyme Based Analytical Chemistry - Nitrate and the U.S. EPAEnzyme Based Analytical Chemistry - Nitrate and the U.S. EPA
Enzyme Based Analytical Chemistry - Nitrate and the U.S. EPA
 
Efficient and Re-usable SAPO Catalyst for the Selective Production of Furans ...
Efficient and Re-usable SAPO Catalyst for the Selective Production of Furans ...Efficient and Re-usable SAPO Catalyst for the Selective Production of Furans ...
Efficient and Re-usable SAPO Catalyst for the Selective Production of Furans ...
 
A new RP-HPLC method development and validation of Sofosbuvir in bulk and pha...
A new RP-HPLC method development and validation of Sofosbuvir in bulk and pha...A new RP-HPLC method development and validation of Sofosbuvir in bulk and pha...
A new RP-HPLC method development and validation of Sofosbuvir in bulk and pha...
 
JBEI Highlights - October 2014
JBEI Highlights - October 2014JBEI Highlights - October 2014
JBEI Highlights - October 2014
 
NOVEL SEPARATION AND QUANTITATIVE DETERMINATION OF LEVOFLOXACIN, PRULIFLOXACI...
NOVEL SEPARATION AND QUANTITATIVE DETERMINATION OF LEVOFLOXACIN, PRULIFLOXACI...NOVEL SEPARATION AND QUANTITATIVE DETERMINATION OF LEVOFLOXACIN, PRULIFLOXACI...
NOVEL SEPARATION AND QUANTITATIVE DETERMINATION OF LEVOFLOXACIN, PRULIFLOXACI...
 
Assessing The Impact Of Agricultural Practices On Phosphorous Availability An...
Assessing The Impact Of Agricultural Practices On Phosphorous Availability An...Assessing The Impact Of Agricultural Practices On Phosphorous Availability An...
Assessing The Impact Of Agricultural Practices On Phosphorous Availability An...
 

Pre-Final Draft 3 Phosphate Prouct Sheet

  • 1. 334 Hecla Street, Lake Linden, MI 49945 tech@nitrate.com | 1.906.296.1130 sales@nitrate.com | 1.906.296.1115 www.nitrate.com Specificity: Sensitivity: Analysis Ranges: Sample Types: Storage Conditions: Active Temperature Range: Buffer Specifications: Enzymatic Orthophosphate Test NECi’s Enzymatic Orthophosphate Test is a highly specific, sensitive and selective assay for the quantitative analysis of soluble reactive phosphate (SRP) in many types of sample matrices. Quantitative orthophosphate analysis is useful for evaluating soil health, environmental health, industry process control, wastewater nutrient levels, and much more. This method is compatible with the persulfate digestion for total phosphorus analysis. Total P kits based on the USGS method will be available soon. Utilizes a recombinant enzyme Purine nucleoside phosphorylase (PNP) to catalyze the reaction of phosphate with substrate 2-amino-6-mercapto-7-methylpurine ribonucleoside (MESG). The resulting 2-amino-6-mercapto-7-methylpurine (AMMP) absorbs at 360 nm for accurate photometric analysis of phosphate. Replaces the ascorbic acid method for more precise analysis. Results in phosphorus units are obtainable with a simple conversion factor. Small reaction volume, minimal sample preparation, & non-hazardous materials or waste. Available in test tube, microplate, & on-site formats or build your own assay with enzyme reagent packs. Orthophosphate 15 - 40 °C 200 mM HEPES | 20 mM MgCl2 pH 7.6 Water Soil Aqueous Extracts Low: 0.5 - 5.0 ppm Standard: 5.0 - 20.0 ppm 0.5 ppm MDL 3+ Years @ -80°C 1+ Year @ -20°C 6 Months @ 4°C
  • 2. 334 Hecla Street, Lake Linden, MI 49945 tech@nitrate.com | 1.906.296.1130 sales@nitrate.com | 1.906.296.1115 A Closer Look at the Reaction Overview of the Method Example Standard Curve H2PO4 - 2-amino-6-mercapto-7-methylpurine ribonucleoside + 2-amino-6-mercapto-7-methylpurine Purine Nucleoside Phosphorylase MESG Ribose-1-phosphate + Wait 10 Min 900 µL Reaction Mixture Combine Reaction Mixture + Sample into test tube 100 µL Sample Fill to 20.5 mL with Buffer Prepare Stock Reaction Mixture 1 mL PNP 1 mL MESG By using a set of phosphate standards of known concentration, a standard curve is used to calibrate the method and quantify phosphate content of unknown samples. When used in conjunction with NECi’s Handheld Photometer, results can be obtained on any Android mobile device. The app software has a pre-programmed standard curve and displays data in ppm phosphate. Measure A-360 of each sample in UV compatible cuvettes Note: ppm Phosphate x 0.3262 = ppm Phosphorus Order online at nitrate.com/store/index.php/phosphate