This document provides information about NECi's Enzymatic Orthophosphate Test for the quantitative analysis of soluble reactive phosphate. The test utilizes a recombinant enzyme to catalyze the reaction of phosphate with a substrate, resulting in a product that absorbs at 360 nm for accurate photometric analysis. It has a high sensitivity down to 0.5 ppm and can analyze various sample types like water, soil, and aqueous extracts. The test involves combining a reaction mixture and sample, incubating for 10 minutes, and measuring absorbance to obtain phosphate concentration using a standard curve.