9. CULTIVO DE PARÁSITOS DE
LEISHMANIA Y CEPAS DE
REFERENCIA
Cepas de referencia de
- L. donovani (DD8)
- L. tropica (K27)
- L. donovani (MHOM/IN/1983/AG83)
28. AUTHOR WHAT THEY SAID OUTCOME
SSCP is one of the basic methods to
identify single base divergence in genes.Gasser
Understanding the molecular
mechanisms of clinical resistance is
crucial for analyse the progress and
extent of drug resistance in the field.
Asutosh
Srivastava
Significant genetic polymorphisms exist
in L. donovani strains in Bihar, India.
You can differenciate resistant and
sensitive SSG Leishmania parasites,
by protein encoding genes, rDNA and
mini-exon.
Downing
29. CLOSURES
• For this pathology, it isn’t enough for the sandflies to transmit the parasites
just by a bite, it is obligatory an intra-macrophage process.
• One can tell apart the resistant Leishmania isolates from the sensitive
Leishmania isolates on the SSCP method.
30. • In order to avoid drug resistance, we need to understand the
polymorphisms regarding each parasite.
• Of the few samples that were positive for the codification of L. tropica,
they could recognize the susceptibility for the SSG. Discovering an
important, but not of huge magnitude diagnosis impression.