14. • SYBR green: dye that fluoresces only when bound to
double stranded DNA (i. e the PCR product)
• Taqman: Gene specific nucleic acid probe (Reporter
and quencher molecules)
quencher absorbs the fluorescence emitted by the reporter
15.
16. Taqman method is that probes with different
coloured reporters can be combined in multiplex
17. Taq DNA polymerase used has a 5′-exonuclease
activity and therefore will begin to chew away at any
sequences in its path (i.e., those probes that have
annealed to the target sequence).