PPT ON Development of a recombinant proteinimmunogen based vaccine against Hepatitis E virus

2. Contents
introduction

3. Immunization,is the process by
which an individual's immune
system becomes fortified against an
agent (known as the immunogen).
Immunization is done through
various techniques, most
commonly vaccination.

4. Vaccinology
The science or methodology of vaccine development is
vaccinology.
A vaccine is a biological preparation that improves
immunity to a particular disease.
A vaccine typically contains an agent that resembles a
diseasecausing microorganism, and is often made from
weakened or killed forms of the microbe.
The agent stimulates the body's immune system to
recognize the agent as foreign, destroys it, and
"remembers" it, so that the immune system can easily
recognize and destroy any of these micro-organisms that it
encounters later.

5. Properties of an ideal vaccine
Provide long lasting immunity.
Should induce both humoral and cellular immunity.
Should not induce autoimmunity or hypersensitivity.
Should be inexpensive to produce, easy to store and
administer.
Vaccines must also be perceived to be safe.
The vaccine vial may contain relevant antigen, adjuvant
(usually alum), preservatives and/or traces of protein
derived from the cells in which the vaccine agent was
cultured e.g. egg protein.

6. Synthetic Peptides
Synthetic peptides are often used as
immunogens because they are quickly and
easily synthesized and are relatively
inexpensive.
The downside is that they have no
secondary, tertiary or quaternary structure,
which can be critical for antibodies used in
flow cytometry (FACS),
immunoprecipitation, immunofluorescence
or blocking antibodies that need to react
with native structures.

7. Types of Vaccines
Killed
Attenuated
Toxoids
Subunit
Recombinant Protien vaccines
Conjugate
DNA
Recombinant Vector Vaccines
Anti-idiotypic Vaccine

8.  Recombinant
protein, a manipulated
protein, which is generated
in various ways to produce
large quantities of protein,
useful commercial
products.
 The formation of
recombinant protein is
carried out in specialized
vehicles known as vectors.
 Recombinant technology is
the process involved in the
formation of recombinant
protein.
 a protein encoded by a
recombinant DNA that has
been cloned in a system
that supports expression of
the gene and translation of
messenger RNA.
 Modification of the gene
by recombinant DNA
technology can lead to
expression of a mutant
protein.
 Proteins coexpressed in
bacteria will not possess
post-translational
modifications, e.g.
phosphorylation or
glycosylation; eukaryotic
expression systems are
needed for this.