The anti-ulcer activity of chloroform and ether extract of Jasminum officinale L. chloroform and ether induced ulcer models in wistar rats. In both models the common parameter determined was ulcer index. Chloroform and ether extract at doses of 200 mg/kg p.o produced significant inhibition of the gastric lesions induced by Pylorus ligation induced ulcer & chloroform and ether induced gastric ulcer.

1. Submitted to - Submitted by -
Dr. Sanchita Kulshrestha Ms. Lokendra Singh
PHD, M.Sc. Biotechnology M.Sc. Biotechnology

2. INTRODUCTION
 JASMINE AS A MEDICINAL PLANT
 CLASSIFICATION:
Kingdom: Plantae
Division: Magnoliophyta
Order: Scrophulariales
Class: Magnoliopsida- Dicotyledons
Family: Oleaceae
Genus: Jasminum
Species: J. officinale
 GENERAL INTRODUCTION
 Botanical Name Jasminum officinale. (Jasminum officinale of India)
 Hindi Name Chameli
 Common Name Common Jasmine, True jasmine, Poet's jasmine
 Part used Leaf’s
Distribution: It is found in Tamilnadu, Andhra Pradesh, Kannad, Chazipur, Sikanderpur and Karnataka, the Jasmine
particularly J.sambac (L.)

3. USES
 Traditional uses:
Leaves were chewed in aphthous, stomatitis, toothache and ulcer in the mouth.
Leaf juice or oil obtained from it was dropped into the ear.
 Medicinal Uses:
 Although rarely used in Western medicine, a jasmine flower syrup for coughs and lungs
was once made. The flowers make a tea that calms the nerves and increases erotic feelings.
 The whole plant is considered effective in expelling worms, in regulating menstrual flow,
and in helping to clear the kidneys of waste.
 In traditional Chinese medicine states that jasmine clears the blood of impurities.
 The oil of the leaf is rubbed on the head to heal the eyes

4. MATERIALS REQURIED
 1. Plant collection
 Jasminum officinale leaves were collected from herbal garden of dravyaguna vigyan, National Institute
of Ayurveda (NIA), Jaipur.
 The plant was identified and authenticated by Botanist of Bilwal Medchem and Research Laboratory Pvt.
Ltd., Siker, Rajasthan, India
 2. Preparation of the extract
 About 60 g of the leaf powder were extracted with 600 ml of chloroform and petrolium ether each by
maceration at room temperature for 7 days.
 The extract dried at 40º C and the yield of the chloroform extract and petrolium ether extract was 26% and
22% respectively.
Ethical Approval: IBIR/IAEC/2017/I/14 from IBIR.

5.  3. Animals
 Albino rats of Wistar strain of either sex weighing between 150 and 200 g were used.
 They were housed in standard cages at room temperature (25±2º C) and provided with food and
water ad libitum.
4. Drugs and chemicals
 Aspirin was obtained from German Remedies Ltd., Mumbai, India and ranitidine from Glen
mark Pharmaceuticals Ltd., Mumbai.
 Chemicals analytical grade.

6. GROUPS
(6 animal per group)
TREATMENT
(For 10 Days)
OTHERE
I Control Received 0.5% w/v CMC solution, p.o. ---
II Aspirin only Received aspirin alone (200 mg/kg body weight,
p.o.)
---
III JOL-CE group Received JOL-CE (200 mg/kg body weight, p.o.) From days 7 to 10, animals of group III, IV, V received
aspirin (200 mg/kg body weight, p.o.), 2 h after the
administration of respective drug treatment (Goel et al.,
1986;Venkataranganna et al., 1998)IV JOL-EE group Received JOL-EE (200 mg/kg body weight, p.o.)
V Ranitidine group Received ranitidine (20 mg/kg body weight, p.o.)

7. METHODS
1. Acute toxicity study:
Six animals for each extract Jasminum officinale leaf chloroform extract (JOL-CE) and ether extract (JOL-
EE)] were used which received a single oral dose (2000 mg/kg, body weight for each extract).
Animals were observed individually at least once during the first 30 min after dosing, periodically during the
first 24 h (with special attention during the first 4 h) and daily thereafter for a period of 14 days.
Observations included changes in skin and fur, eyes and mucous membrane (nasal) and also respiratory rate,
circulatory, and central nervous system (ptosis, drowsiness, gait, tremors and convulsion) changes, Mortality,
if any, was determined over a period of 2 weeks as per the OECD guideline 423, 2002.

8. 2. Aspirin induced ulcer in rats:
Solutions of JOL-CE, JOL-EE, aspirin and standard antiulcer drug, ranitidine (Dharmani et al., 2005; Gupta et
al., 2005) were prepared in 0.5% w/v sodium carboxy methyl cellulose (CMC) suspension as vehicle and
administered orally once daily at a volume of 10 ml/kg body weight.
The animals were divided into five groups, consisting of six rats in each group (having H, B, T, HB, BT, and
HT marking respectively).
Animals in all the groups were fasted for 18 h after the respective assigned treatment (i.e. after 10th day) and
were anaesthetized with an aesthetic ether. The abdomen was opened by a small midline incision below the
xiphoid process and pylorus portion of stomach was lifted out and ligated (Shay et al., 1945).
Four hours after pylorus ligation the rats were sacrificed and the stomach was removed.

9. STATISTICALANALYSIS
 The experimental results of aspirin induced ulcer in rats were expressed as mean ± S.E.M.
Data were analyzed by two way analysis of variance (ANOVA) followed by Tukey's
multiple comparisons test using Graph pad Prism 7 software, with the level of significance
set at P < 0.05 (at 95 % confidence interval).

10. OBSERVATIONS
Tukey's multiple comparisons test Mean Diff. 95.00% CI of diff. Difference is
significant?
Summary P Value
Control vs. Aspirin -33.9 -52.7 to -15.1 Yes *** <.001
Control vs. JOL-CE -9.87 -28.7 to 8.95 No ns .595
Control vs. JOL-EE -6.89 -25.7 to 11.9 No ns .849
Control vs. Ranitidine -2.19 -21 to 16.6 No ns .998
Aspirin vs. JOL-CE 24.1 5.23 to 42.9 Yes ** .005
Aspirin vs. JOL-EE 27 8.21 to 45.9 Yes ** .001
Aspirin vs. Ranitidine 31.7 12.9 to 50.6 Yes *** <.001
JOL-CE vs. JOL-EE 2.98 -15.8 to 21.8 No ns .992
JOL-CE vs. Ranitidine 7.68 -11.1 to 26.5 No ns .790
JOL-EE vs. Ranitidine 4.7 -14.1 to 23.5 No ns .958

11. Fig. 1: Section of ulcerated stomach obtained from rats of control group treated with aspirin-induced ulcer model in rats after 10 days of treatment (n=6 in each
group). HE 100×.
Fig. 2: Section of ulcerated stomach obtained from rats of group treated with ranitidine (20 mg/kg) in aspirin -induced ulcer model in rats after 10 days of treatment (n = 6 in each
group). HE 100×.

12. CONCLUSION
 The presented results offer supporting evidence for effective use of selected plant
extracts for peptic ulcer activity. Jasminum officinale naturally possess variety of
therapeutic agents but properties depend on the component of the plant, the
system used to isolate these agents and method followed to evaluate the particular
character. In the current study ether extract of Jasminum officinale leaf exhibited
comparatively better activities than chloroform extract in the assay seemingly due
to efficient extraction of phytochemicals.

13. THANK YOU