This document provides an overview of Raman spectroscopy, including its principle, instrumentation, and applications. It begins with a brief history noting key milestones like the 1928 discovery by C.V. Raman which earned him the Nobel Prize. The principle section explains that monochromatic laser light interacts with a sample, with most light undergoing elastic Rayleigh scattering while a small amount undergoes inelastic Raman scattering. This provides information about molecular vibrations and structure. The instrumentation section notes the basic components needed to perform Raman spectroscopy. Finally, applications are discussed across various fields like chemistry, materials science, pharmaceuticals, and biology where it is used for qualitative and quantitative analysis.

1. RAMAN
SPECTROSCOPY
Presented by​
Md Ariful Islam
M. Pharm 2nd Semester
Department of Pharmaceutical Sciences
Dibrugarh University
Advanced Spectral Analysis(MPC 201T)
Principle, instrumentation and applications

2. CONTENT
Introduction​
Principle of Raman Spectroscopy
​Instrumentation
Applications
​Summary
References​

3. INTRODUCTION
Milestones of Raman spectroscopy
• 1923 : Inelastic light scattering predicted by A. Smekal
• 1928 : C.V. Raman and K.S. Krishnan see "secondary radiation"
from neat solvents (Nobel Prize 1930)
• 1960 : Invention of laser - Breakthrough for Raman experiments
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5. PRINCIPLE
Raman Spectroscopy

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Interaction of light with a sample
Laser light (monochromatic) interacts with sample
Laser light can be:
• Absorbed
• Transmitted
• Scattered
• →Elastic scattering / Rayleigh (green)
• →Inelastic scattering / Raman (red and blue

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12. INSTRUMENTATION
Raman Spectroscopy

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15. APPLICATION
Raman Spectroscopy

16. APPLICATION
Raman Spectroscopy

17. APPLICATION
Raman Spectroscopy

18. APPLICATION
Raman Spectroscopy

19. APPLICATION
Raman Spectroscopy

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21. APPLICATION
Raman Spectroscopy

22. SUMMARY 22
• Raman spectroscopy analyzes the interaction of light with matter to gather
information about molecular structure and composition.
• It is based on the Raman scattering phenomenon discovered by Sir C.V. Raman.
• Monochromatic light is directed onto a sample, and the scattered light is analyzed.
• The majority of scattered light undergoes elastic scattering (Rayleigh scattering),
while a small fraction undergoes inelastic scattering (Raman effect).
• Inelastic scattering occurs due to interactions with molecular vibrations and
rotations.
• The energy shifts in the scattered light provide insights into the chemical bonds,
molecular structure, and composition of the sample.
• A Raman spectrum is obtained by analyzing the energy shifts in the scattered light.
• Raman spectroscopy is widely used in chemistry, materials science,
pharmaceuticals, forensics, and biology for qualitative and quantitative analysis.

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"The Raman effect has opened up new vistas
in the field of molecular spectroscopy,
providing an invaluable tool for the study of
molecular structure and dynamics."
Quotes

24. REFERENCES
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1. C.V. Raman & K. S. Krishnan, A New Type of Secondary Radiation, Nature 121, 501- 502 (1928)
2. Smith & G. Dent, Modern Raman Spectroscopy: A Practical Approach 1st ed. , Wiley (2005

25. THANK YOU