1. ANIMAL CELL BIOTECHNOLOGY
AND GENETICS
Presented by:
ANURAN MAJUMDER.
Semester: 4th
Roll: 14UBE007
Department: BIOENGINEERING
TOPIC:
PROBE DESIGNING WITH
SIGNAL MOLECULE

2. WHAT IS PROBE ?
 A labelled DNA or RNA molecule.
 They are single stranded.
 Used to search sequence of interest from the mixture of
nucleic acid.
 Important : Probe is a complementary to the sequence of
interest.

3. TYPES OF PROBES :
Enzymatic probe.
These probes detect target proteins using enzymes for detection.
 HOW THIS PROBE WORK?
1. Enzyme are conjugated to another protein (eg : biotin)
2. The protein binds to target molecules.
3. A substrate of the specific enzyme is added.(DAB).
4. A visible reaction is formed.( It gives blue colour)

4. TYPES OF PROBES :
 Enzymatic probe.
 Florescence probe.
 Radioactive probe.
Fluorescent molecules, also called fluorophores
or fluors responds distinctly to light and are
used to detect protein molecules /target
molecules.
A radioisotope, is used in the probe to label the
nucleic acid fragments of our interest.