Survey of fungi from archaeological digs at ayodhya

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A study of the fungi from the soil of 8.5 m deep archaeological digs- differentiated into 18 strata- at Katra in Ayodhya of Faizabad district (U.P.) has been made. The upper stratum was found to harbour a greater no of fungi, which decrease in quality and quantity towards the lower profiles up to 4.5-5.35 m and finally to nil. The mebers of phycomycetes and ascomycetes were confind to the upper few strata while deuteromycetes were diversified in their distribution.

Survey of fungi from archaeological digs at ayodhya

Survey of fungi from archaeological digs at ayodhya

Survey of fungi from archaeological digs at ayodhya

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Morchella, the true morels, belonging to Helvellaceae family of class Ascomycetes, are amongst the most highly priced fungi in the world. Their artificial production is still a challenge, even though patents for their cultivation do exist. The tissue of Morchella sp. was transferred aseptically to Potato Dextrose Agar medium (peeled, sliced and boiled potato, 200 g; dextrose, 20 g; agar, 20 g L-1) to grow hyphae. The mycelium showed fastest growth as compared to other edible mushrooms. It covered entire area of Petri plate (90 mm) within 4-5 days with the growth rate of 18- 22.5mm/day. A unique growth pattern i.e., vertically oriented mycelia were observed. Brown coloured pigmentation in the culture was also observed during the study. The basal media for spawn (wheat grains; Glucose, 1%, CaCO3, 2%; CaSO4, 1.5% and MgSO4, 1%) was aseptically inoculated with the mushroom culture. The spawn substrate was colonized by mushroom mycelium in 7-8 days. The sclerotia were formed in unused (old) spawn. Wheat straw was used as a substrate for mushroom cultivation. It was supplemented with wheat bran, 20%, Glucose (1%) and MgSO4 (1%). It showed prolific growth when it was seeded by mushroom spawn using jar method. Once substrate was fully covered with mushroom mycelium, casing was applied. Sclerotia were successfully obtained after 14 days of incubation in our experiment both in the substrate and casing soil but failed to give rise to fruiting primordial. Further research is going on to domesticate this species in our country.

Sclerotia production a way ahead to morchella Cultivation

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Morchella, the true morels, belonging to Helvellaceae family of class Ascomycetes, are amongst the most highly prized fungi in the world. Their artificial production is still a challenge, even though patents for their cultivation do exist. The tissue of Morchella sp. was transferred aseptically to Potato Dextrose Agar medium (peeled, sliced and boiled potato, 200 g; dextrose, 20 g; agar, 20 g L-1) to grow hyphae. The mycelium showed fastest growth as compared to other edible mushrooms. It covered entire area of Petri plate (90 mm) within 4-5 days with the growth rate of 18-22.5mm/day. A unique growth pattern i.e. vertically oriented mycelia were observed. Brown coloured pigmentation in the culture was also observed during the study. The basal media for spawn (wheat grains; Glucose, 1%, CaCO3, 2%; CaSO4, 1.5% and MgSO4, 1%) was aseptically inoculated with the mushroom culture. The spawn substrate was colonized by mushroom mycelium in 7-8 days. The sclerotia were formed in unused (old) spawn. Wheat straw was used as a substrate for mushroom cultivation. It was supplemented with wheat bran, 20%, Glucose (1%) and MgSO4 (1%). It showed prolific growth when it was seeded by mushroom spawn using jar method. Once substrate was fully covered with mushroom mycelium, casing was applied. Sclerotia were successfully obtained after 14 days of incubation in our experiment both in the substrate and casing soil but failed to give rise to fruiting primordial. Further research is going on to domesticate this species in this part of country.

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Aim: The efficient mushroom production involves many factors of which spawn production, culture methods and substrate selection are of prime importance. The research has, therefore, been carried out to standardize these aspects in present communication, for production of Pleurotus florida Strain P1. Materials and Methods: Various cereals (maize, oat, barley) and millets (Italian millets, Little millet, Pearl millet) were assessed against wheat grain spawn for enhancing yield and biological efficiency of mushroom. Most suitable wheat straw component among fine pieces of leaves and leaf sheath (0.2 cm), coarse pieces of leaves and leaf sheath (0.4 cm), small (1.0 cm) and large pieces of the stem (1.7 cm) were also evaluated for the manifestations above. Various culture methods viz., bag, column, wall and tray culture and few substrate mixtures (corncob + wheat straw, mango sawdust + wheat straw and rice husk + wheat straw) have also been taken to increase yield performance of mushroom. Results: The result showed that barley and oat grains produced good quality spawn in terms of quick mycelial running on the grain surface (14 days each). These spawn produced acceleration of spawn running and increased yield, as compared to other types of spawn. When different components of wheat straw were evaluated for the yield performance of mushroom, the course pieces of the stem (1.0 cm) proved the most appropriate component in relation to very low/zero contamination along highest yield (819 gm) and biological efficiency (163.8%). In contrast, wheat straw and corn cob were recognized as the best substrate combination with highest yield (707 gm) and biological efficiency (141.4%) of mushrooms. Among different culture techniques used, only the column method gave significant yield (930 gm) and biological efficiency (186%) with appropriate mushrooms sizes from packaging point of view. Conclusion: The results obtained during the study revealed that by adopting the composite approach, the growers enhance mushroom production in manyfolds. The mushroom is cultivated by following column method. The wheat straw devoid from pieces of leaf & leaf sheath should be utilized as a substrate with corncob combination. It should be inoculated with oat/barley spawn to higher yield.

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A new filter for safe view of Solar Eclipse

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Bottle culture: A suitable method for oyster mushroom cultivation

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Two wastes from animal origin, viz., human hairs and egg shells were evaluated for different manifestations of white oyster mushroom, Pleurotus florida. The mushroom utilized both the substrates for their growth and sporophore formation. The mycelial growth was significantly (P=0.05) faster on egg shell (18 days) as compared to human hair (23 days). The crop of mushroom was harvested in four flushes where human hairs showed higher yield and biological efficiency of mushroom (165 gm, 33%) than egg shells (155 gm, 31%), respectively. In respect of yield parameters such as yield, biological efficiency, number of mushroom fruit bodies and average weight of sporophores, both the substrates were statistically at par to each other. Utilization of human hairs egg shells by P. florida reveals a new strategy for mycoremediation of these wastes.

Comparison of Growth and Development of Pleurotus florida against Wastes from...

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Eight different kinds of papers, viz., glaze paper, brown paper, news paper, magazine paper, chart paper, kite paper, rough copy paper and A-4 size printing paper, and two types of cardboard viz., corrugated cardboard and card board were evaluated for different manifestations of white oyster mushroom Pleurotus florida Strain-P1. Among them news paper was later treated as a control. The mushroom was utilized all the substrates for their growth and sporophore formation. Majority substrates were taken almost equal time for spawn run, primordial development and fruiting bodies maturation. The measured parameters were net yield (Weight of fresh mushrooms), biological efficiency, number of fruiting bodies produced and average weight of sporophores varied among themselves. The crop of mushroom was harvested in three flushes where yield and biological efficiency ranged 190-495 gm, 38-99% for the substrate used. Magazine paper (450 gm; 90%) and card board (495 gm; 99%) produced significant (P=0.05) yield and biological efficiency over control. They also produced significant number of mushroom fruit bodies (56 and 64, respectively). Corrugated cardboard (10.29 gm) was found significant in terms of average weight per sporocarp. The percentage yield of different substrates was also evaluated. Among the substrates, card board contributed 14% of total mushroom production followed by magazine paper (13%) and news paper (12%).

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Aims: Cultivation of oyster mushrooms has increased vastly in a global scale during last few decades. Contaminants and indigenous microflora present in the substrate may led to the low productivity of mushrooms. Keeping this in mind, the present study was under-taken with slight modification in substrate preparation technique to eliminate dust particles from the substrate and to assure contamination free mushroom production. Study Design: Comparative evaluation between modified approach and control beds. Place and Duration: The study was carried out in Shri Laxman Prasad Pyare Lal Agro Products, Ayodhya during 2017. Methodology: The wheat straw substrate was immersed in the drum containing tap water, mixing properly and allowed to stand for 10-15 min to settle down the dust particle in the bottom of the drum. After that, the floating substrate was transferred to the slant surface so that the extra water was decanted off. This substrate was put in to the steam sterilised gunny bag and steeped in the chemical solution consisting of Formaldehyde (500 ppm) and Bovistin (75 ppm) for 18 h. For the Short Communication Siddhant et al.; AJAHR, 2(2): 1-5, 2018; Article no.AJAHR.44907 2 control, fresh wheat straw was put in to the steam sterilised gunny bag and steeped directly in the chemical solution as suggested above. After removal of excess water, the substrate was used for spawning with inoculums of Pleurotus sajor-caju, Strain- Malaysia. Results: Beds with modified approach showed a complete absence of contaminants during entire crop cycle which showed the effectiveness of modified method while beds treated as control showed little incidence of various contaminants viz., Alternaria alternata, Aspergillus fumigatus, Curvularia sp., Penicillium sp., and Rhizopus stolonifer with 6.67-20.00 percent incidence. Conclusion: The results revealed that the modified method should be considered to contamination free mushroom production.

A Modified Approach in Substrate Preparation Technique for Small Scale Oyster...

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Two wastes from animal origin, viz., human hairs and egg shells were evaluated for different manifestations of white oyster mushroom, Pleurotus florida. The mushroom utilized both the substrates for their growth and sporophore formation. The mycelial growth was significantly (P=0.05) faster on egg shell (18 days) as compared to human hair (23 days). The crop of mushroom was harvested in four flushes where human hairs showed higher yield and biological efficiency of mushroom (165gm, 33%) than egg shells (155gm, 31%), respectively. In respect of yield parameters such as yield, biological efficiency, number of mushroom fruit bodies and average weight of sporophores, both the substrates were statistically at par to each other. Utilization of human hairs and egg shells by P. florida reveals a new strategy for mycoremediation of these wastes.

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A demonstration of mushroom cultivation on bracket fungi

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During an extensive survey of fungi from Faizabad (U.P.), the author came across two important diseases, viz., wet rot of Cereus hexagonus caused by Periconia byssoides Pers ex Me'rat and fruit rot of Syzygium cumini (L.) Skeels caused by Rhizopus nigricans Ehrenb. ex Corda, new to India. The specimen has been deposited in the Department of Botany, K.S.S. P.G. College, Faizabad under Accession No. RSK 415 and 558.

Wet rot disease of Cereus hexagonus and fruit rot of Syzygium cumini, new to ...

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Record of periconia byssoides on four o clock plant from india

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Periconia byssoides is known to parasite a number of plants in India but so far not reported on Capsicum annuum L. The small spherical to irregular spots of light brown colour gradually enlarged and merged subsequently to form continuous dark brown to black patches on stem, leaves and fruits. The whole plant were soon infected and ultimately dried within 20-25 days. The damage was about 70-85 per cent. The fungus was isolated on PDA medium by usual methods. The specimen has been deposited (No. RSK-142) in the herbarium of Botany Department, K.S.S. P.G. College, Faizabad. Cited this as: R.S. Kanaujia and R.S. Verma (1979): Periconia rot of Capsicum annuum in India. Indian Phytopathology. 32(3): 445.

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In December 1978, the author observed the stored fruits of Citrus reticulata Blanco severely infected by a fungus causing soft rot in local fruit market of Faizabad. The entire fruit surface was densely covered by the fungus causing the rot. The fungus was isolated on PDA medium and identified as Trichoderma lignorum (Tode) harz. In India, T. lignorum has been found on various substrates, viz., soil, rhizosphere region of Pennisetum typhoides, paddy straw of mushroom beds, phyllosphere of Impatiens balsamina and Citrus crysocarpa but not on Citrus reticulata and the present association is a new record for India. Cited this as: R.S. Kanaujia (1979): Fruit rot of mandarin orange. Indian Phytopathology. 32(3):450.

Fruit rot of mandarin orange

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Short hole disease of Alocasia indica Schott, caused by Phoma exigua Desm., earlier unknown to India has been reported from Faizabad. The infection was chiefly confined to the margins of lamina. It started as small nonchlorophyllous spots near the fronds. The spots gradually enlarged irregularly upto 2-3 cm sq. area of the leaf, merged and in final stage, necrosis and then short holes appeared within a month. The pathogenicity tests performed by standard methods proved pathogenic nature of the fungus.

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Cotton crops are vulnerable to a variety of sucking pests, which can severely impact plant health, yield, and fiber quality. These pests primarily feed on plant sap, extracting nutrients directly from the plant's vascular system. Here's a breakdown of some of the most significant sucking pests in cotton cultivation: Aphids: Cotton aphids or melon aphids can cause direct damage by sucking sap and indirect damage by secreting honeydew, which encourages sooty mold growth. This can interfere with photosynthesis and weaken the plant. Aphids can also transmit viral diseases. Whiteflies: Two species, the silverleaf whitefly and the bandedwinged whitefly, are particularly troublesome. They not only suck sap from the underside of leaves, causing yellowing and leaf drop, but their honeydew excretion promotes sooty mold and they can transmit several plant viruses. Thrips: While thrips can chew on plants, their primary damage to cotton is through sucking. They attack the cotton plant during its seedling stage, which can stunt growth and reduce vigor. Thrips are also capable of transmitting the Cotton Bud disease. Spider Mites: These are not insects but arachnids. Spider mites, such as the two-spotted spider mite, suck cell contents from the leaves, leading to speckled discoloration and potentially significant leaf loss if infestations are severe. Leafhoppers: Including various species, leafhoppers can cause direct damage through feeding, which results in leaf curling and stunted growth. They can also be vectors for plant diseases. Mealybugs: These pests are less common but can be problematic, especially in clustered planting conditions. They suck sap and secrete honeydew, which leads to sooty mold. Mealybugs can also spread viruses. Stink Bugs: Although primarily known for their chewing mouthparts, certain stink bugs can cause damage similar to sucking pests by injecting saliva into the plant and sucking out nutrients, leading to boll damage and stained lint. Management Strategies: Cultural Controls: This includes practices such as crop rotation, using resistant varieties, and managing planting and harvesting times to avoid peak pest populations. Biological Controls: Beneficial insects like lady beetles, lacewings, and predatory mites can naturally control sucking pest populations. Parasitic wasps also play a role in controlling aphid and whitefly populations. Chemical Controls: Insecticides may be used but should be chosen carefully to minimize resistance development and preserve beneficial insects. Systemic insecticides can be particularly effective against sucking pests. Integrated Pest Management (IPM): Combining multiple control strategies based on monitoring and thresholds to apply the most effective and environmentally sensitive approach. Effective management of sucking pests in cotton requires a thorough understanding of the pest species present, their life cycles, and the ecological balance of the field environment.

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