extraction of keratin from human hair in this ppt you are going to learn about extraction of keratin from the human hair waste with a very simple method and the method is economic

1. EXTRACTION OF KERATIN FROM HUMAN
HAIR WASTE
PREPARED BY :
• DEEPAK KUSHWAHA
Student :
VIVA institute of pharmacy
Virar (east)

2.  INTRODUCTION
 STRUCTURE OF HAIR
 STRUCTURE OF KERATIN
 OBJECTIVE OF WORK
 RESEARCH PROBLEM
 METHODOLOGY
 APPLICATION
CONTENTS

3. INTRODUCTION
 Keratinisatypeofscleroproteinmadeupofaminoacidsthatarelinkedbybothintramolecularand
intermolecularinteractions,includingdisulfidebonds,ionicbonds,hydrogenbonds,andhydrophobic
interaction.
 Human hair is one of the most abundant natural fibers rich in keratin and one of the most
abundant waste materials produced by humans.
 The keratin extracted from humans are found to be more biocompatible, less immune
stimulating when used in transplantation and are readily biodegradable.

4. STRUCTURE OF HUMAN HAIR
 Hair is a filamentous biomaterial consisting
mainly of proteins in particular keratin.
 Externally, hair is thin, flexible tubes of dead, fully
keratinized epithelial cells, whereas inside the
skin, it is a part of individual living hair follicles,
cylindrical epithelial down growths into the
dermis, and subcutaneous fat, which enlarge at
the base into the hair bulb surrounding the
mesenchymal derived dermal papilla.
 The structure of human hair consist of
 Medulla
 Cortex
 Cuticle

5.  Keratin is one of the most important structural proteins in nature and is widely found in the
integument in vertebrate.
 It is classified into two types:
 α -keratin
 β-keratin
Structure of keratin

6. • alpha keratin is high with alanine,
leucine, arginine and cysteine. These
amino acids contribute to form a right-
handed helix structure and a left-handed
helical structure
• alpha keratin occurs in hair, horns, nails
and the epidermal layer of skin
 β-keratin
 α -keratin
• Beta keratin is a structural
protein that occurs mainly in the
epidermis of reptiles.
• it occurs as components in the
epidermal stratum corneum that
is rich in stacked beta pleated
sheets

7. OBJECTIVE
Human hair is considered a waste material in most parts. Disposal of human hair
is a big problem they do not decompose easily it takes 100 years to decompose
and affect the integrity of soil i.e. nitrate leaching into the ground and affect the
environment when buried it produces harmful gasses like ammonia, Sulphur
dioxide, phenol all these waste can be effectively minimizing by using hair for
keratin extraction process involves treating hair with certain chemical results in
production of keratin.

8. Human hair is
non-
biodegradable
and one of the
leading causes
for many
environmental
hazards.
Hair is thrown
into rivers, lakes,
dumping yards
and slowly
decomposes over
several years..
2.444 million
tons of hair is
dumped into
the oceans and
landfills.
Burning human
hair and waste
produces odorous
and toxic gasses
such as NH3,
carbonyl sulfide,
H2S, sulphur
dioxide.
In Barbershop
Human hair is
thrown away, no
matter how
harmful. The reset
process requires an
appropriate mode.
RESEARCH PROBLEM

9. METHODOLOGY
COLLECTION OF HAIR SAMPLE
HAIR DISSOLVED IN NaOH SOLUTION
SAMPLE IS MIXED AT 40 ºC FOR A PERIOD OF 1-2 HOURS
THE MIXTURE WAS FILTERED BY USING WHATMAN FILTER PAPER
CENTRIFUGED AT 5000 RPM FOR A PERIOD OF 10 MINUTES

10. NEUTRALISATION WITH 1N HCL AND PH WAS BOUGHT TO 2-3.5
KERATIN WAS FILTERED FROM THE PRECIPITATE AND DRIED AT 45 ºC, FOR 2 HOURS
KERATIN IS RECOVERED AND PULVERIZED
THE SOLUTION WAS KEPT UNDISTURBED FOR 2 HOURS IN ORDER TO PRECIPITATE OUT THE KERATIN
KERATIN IS RECOVERED AND PULVERIZED
THE KERATIN SAMPLES WERE SUBJECTED TO NINHYDRIN TEST AND FTIR ANALYSIS

11. Methodology
for keratin
extraction

12. CHARACTERIZATION
 PROTEIN CHARACTERIZATION
 FOURIER TRANSFORM INFRA-RED SPECTROSCOPY (FTIR)
 NUCLEAR MAGNETIC RESONANCE (NMR)
 X-RAY DIFFRACTION
 SDS-PAGE ANALYSIS

13. APPLICATIONS
 BIOMEDICAL - Keratin-based materials exhibit great mechanical durability, are extremely
biocompatible and are easily biodegradable. Keratin-based hydrogels have shown
promising results as biomaterials in various biomedical applications.
 FERTILIZER- The keratin material can be used to enrich soil with both organic and inorganic
nutrients. Keratin is an organic nitrogen fertilizer that can be used to feed aquatic plants,
flowers, and vegetables.

14.  COSMETIC - The keratin hydrolysates are used in various cosmetic applications, such as hair
and skin applications. The keratin pep-tides improve hair moisture and provide shine and
softness to the hair.
 BIOSORBENT - Keratin biomaterials aid in eradicating heavy metals from water. These
biomaterials operate through active polar sites on their surface to attract the charged metal
ions via the physical and chemical surficial mechanism

15. 1. J. Wang, H. Gao, C. Qin, Z. Zhao, H. Yuan, J. Wei, and Y. Nie, “Experimental and theoretical study on the extraction of
keratin from human hair using protic ionic liquids,” Journal of Molecular Liquids, vol. 368, p. 120626, 2022.
2. Wang X, Shi Z, Zhao Q, Yun Y. Study on the structure and properties of biofunctional keratin from rabbit hair.
Materials. 2021 Jan 14;14(2):379.
3. A pilot scale study on the extraction & optimization of keratin from human hair – an adapted strategy for the control
of environmental menace. J Environ Treat Tech. 2020 Dec 10;9(1):342–8.
4. B. Buffoli, F. Rinaldi, M. Labanca, E. Sorbellini, A. Trink, E. Guanziroli, R. Rezzani, and L. F. Rodella, “The human hair:
From anatomy to physiology,” International Journal of Dermatology, vol. 53, no. 3, pp. 331–341, 2013.
5. Unnikrishnan, Gayathri, and Vijayaraghavan Ramasamy. “Extraction of Keratin from Human Hair with Production of
Biofertilizer from Waste Liquid of Hair Extraction and Its Efficient Application on Growth Yield of Abelmoschus
Esculentus L.” Asian Journal of Biological and Life Sciences, vol. 9, no. 2, Manuscript Technologies, Sept. 2020, pp.
119–28.
6. Chilakamarry, Chaitanya Reddy, et al. “Extraction and Application of Keratin from Natural Resources: A Review.” 3
Biotech, vol. 11, no. 5, Springer Science and Business Media LLC, Apr. 2021
7. Method “A Pilot Scale Study on the Extraction and Optimization of Keratin from Human Hair – an Adapted Strategy for
the Control of Environmental Menace.” Journal of Environmental Treatment Techniques, vol. 9, no. 1, Dorma Trading
Est, Dec. 2020, pp. 342–48.
REFERENCES

16. THANKYOU!
YOUR JOURNEY OF KERATIN EXTRACTION FROM HAIR ENDS HERE !