Jatropha Hybrid Breeding Platform by Bionic Palm, Ghana
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Jatropha Hybrid Breeding Platform by Bionic Palm, Ghana

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Bionic Palm Ltd., Ghana, is managing the world's most advanced Jatropha breeding program for non-toxic, high yielding hybrid cultivars. With the core research station located in Ghana, Bionic Palm ...

Bionic Palm Ltd., Ghana, is managing the world's most advanced Jatropha breeding program for non-toxic, high yielding hybrid cultivars. With the core research station located in Ghana, Bionic Palm offers localized client breeding services developing on-site Jatropha varieties fully adapted to the clients project conditions and strategy. More about client site breeding services here: http://wp.me/P2MoGN-4b

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Jatropha Hybrid Breeding Platform by Bionic Palm, Ghana Jatropha Hybrid Breeding Platform by Bionic Palm, Ghana Presentation Transcript

  • BIONIC JATROPHA BREEDING PLATFORM All pictures of interspecific F1 hybrid courtesy of Ton Rulkens, Flickr Defining the planting material for Bionic JcL 3.0 © 2011, 2012 Bionic Fuel Knowledge Partners Inc., Oswego, NY, USA 1
  • ABBREVIATIONS USED PoC = proof of concept JcL = Jatropha curcas Linn Jint = Jatropha integerrima Joth = Other Jatropha species NTMA = Non-toxic Mexican accession (of JcL) PE = Phorbol ester (critical toxin in JcL) F1, F2, F3… = generations of hybrids BC1, BC2,… = generations of hybrid back crossing with the original parent population FC1, FC2,… = generations of forward crossing with other than parental accessions/species HX(.X) = hybrid family (exact member number) HCXXXX = hybrid clone of XXXX 2 © 2011, 2012 Bionic Fuel Knowledge Partners Inc., Oswego, NY, USA
  • BACKGROUND – GLOBAL RESEARCHMost important citations from global research literature: “These data suggest that the chances of improvements in commercially desirable traits of J. curcas are higher through interspecific breeding rather than the intraspecific breeding approach, which is limited by the low genetic variability in globally distributed accessions of J. curcas.” (4) “The studies (…) indicate the limitations of using intra-specific breeding for J. curcas improvement; as exemplified by the failure to obtain low PE hybrids in crosses between J. curcas toxic accessions from India and the NTMA” (4) “…analysis of the (JcL x Jint) F1 plants revealed advantages gained through hybridisation (…) including low PE levels. A back cross with J. curcas NTMA further reduced the PE levels comparable to NTMA – a highly desirable target. Our analysis revealed higher genetic distance between J. curcas and J. integerrima than between J. curcas accessions.” (4) “These hybrid (JcL x Jint) clones also expressed superiority in terms of early flowering and fruiting coupled with early yield, which thus lends scope for further promotion and utilization of Jatropha as a successful biofuel crop.” (6) “For J. curcas to be a commercially viable source of biodiesel much research and development is needed and little should be expected from the present widespread planting of wild varieties.” (4) “If the seed cake were rendered non-toxic and could be used as animal feed, the profitability of cultivating Jatropha, which was more expensive than diesel in India in 2005, would be „„dramatically increased.‟‟ (5) 3 © 2011, 2012 Bionic Fuel Knowledge Partners Inc., Oswego, NY, USA
  • BACKGROUND – OWN STUDIES Localization is crucial for commercial planting material as the genotype x ecosystem (GxE) interdependence is high in JcL Epigenetics play a critical role for JcL commercialization being the only available sound explanation for many aspects of the species‟ behavior JcL intraspecific hybridization usually results in very high levels of heterosis if parents exhibit genetic diversity Non-toxic, high yielding hybrids are possible and have a series of ground breaking advantages for the future Non-toxic seed cake is a high value animal feed adds an absolute minimum of US$ 500 per ha to the JcL bottom line Reported barriers to hybridization within the Jatropha genus often prove untrue Proven breeding paths for JcL are available:  Heterosis breeding offers quick path to early sucesses  JcL x Jint has been tried successfully at different instances and shows a high success probability in terms of superior yield and other favorable traits  Adding other Jatropha species increases possibilities even further, but very limited successes have been reported  BPL‟s ongoing proof of concept aims to close knowledge gaps on interspecific breeding routes  Importance of adding true wild accessions from center of origin has been clarified Outlook: There is potential to develop JcL into an annual industrial crop, a route which would resemble the development of Castor decades ago 4 © 2011, 2012 Bionic Fuel Knowledge Partners Inc., Oswego, NY, USA
  • BACKGROUND - CONCLUSIONS JcL is currently an undomesticated wild plant previously used for living hedges Genetic variance within typically (“commercially”) used JcL accessions is too limited (literature)  The germplasm base needs to be significantly expanded for successful breeding results  Introduction of high heterozygosity through interspecific crossing Is BPL’s primary route of choice  J integerima is the species we prioritize, others will follow  Successful interspecific hybrid paths reported in literature  Truly wild accessions from Central America offer an alternative, intraspecific breeding approach (heterosis)  Collected by dedicated expeditions, several have been made available to this program by Geneticlab et. al.  Genetic analysis shows significantly higher heterozygosis compared to the usual homozygosis found in most “commercial” JcL  Many are low/non-toxic  Advantage for time to market due to significant heterosis (hybrid vigor) effects  Induced polyploidy is an advanced breeding tool we are investigating Stable inbred lines of all selected parental material need to be established  (semi) commercial and wild  toxic and non-toxic  selected future hybrid lines Advanced genetic technologies need to be applied  Selection of best suited parental material  Marker assisted breeding  Identification of clear gene-trait relationships is the next step Breeding for horizontal resistance  Significantly reduce the cost of pesticides 5 © 2011, 2012 Bionic Fuel Knowledge Partners Inc., Oswego, NY, USA
  • GENETIC MAPPING SUPPLIES GUIDANCE Central American landraces Commercial varieties (cultivated in Africa and India) Outgroups (Jatropha spp.) 6 © 2011, 2012 Bionic Fuel Knowledge Partners Inc., Oswego, NY, USA
  • APPROACH GREATLY DIFFERS FROM OTHERS Nobody can effectively analyze hundreds or even thousands of accessions phenotypically and genetically (as widely claimed)  Most accessions are genetically identical or very closely clustered  In JcL high phenotypic differences don‟t necessarily correlate with high genetic differences  A wide spread misconception by current JcL breeders  Reasons not clear yet, but epigenetic effects are the most assumed explanation available Effective selectiveness guarantees execution of successful breeding paths  A small core collection of proven genetically divers material has been established at BPL  Collection will be expanded by new hybrids and additional wild accessions over time  Guidance from literature and scientific reports is integrated  Active investigations for sought after properties will be initiated when required  A global network with like minded JcL growers/breeders is maintained Cooperation with scientific researchers  Genetic marker analysis and development  Biochemical analysis  Integration of latest scientific research findings  Program accompanied by long term scientific research 7 © 2011, 2012 Bionic Fuel Knowledge Partners Inc., Oswego, NY, USA
  • PROGRAM OBJECTIVES Stringent commercial approach to scientifically based JcL hybrid breeding A clear proof-of-concept on the most promising approaches by mid 2012  Reduced risk  Reliable planning base A fast track success by mid 2013 (first generation non-toxic hybrids ready for field trials) Marketable success stories by mid 2012 to support critical funding initiative  Funding initiative based on proof-of-concept results  Promising crosses in the early generations have to exhibit genetic improvements for important traits  Only reproducible improvements backed by genetic analysis are relevant  Public news flow to be established before the end of the PoC Mandatory traits  Non-toxic  Further heat treatment for anti nutritional factors will be always required (similar to soya)  High yielding material for West African ecological environments Further preferential traits  Horizontal pest resistance (mealy bug!)  Oil content & composition  Biomass production  Uniformity in size and shape (mechanical harvesting)  Synchronous flowering and fruiting cycles (mechanical harvesting) 8 © 2011, 2012 Bionic Fuel Knowledge Partners Inc., Oswego, NY, USA
  • INTERSPECIFIC JCL HYBRIDS REPORTED J curcas x J integerrima  Many detailed reports  Verified by BPL J gossypifolia x J curcas  Reciprocal is J tanjorensis (sterile)  Patented by Nandan  Verified by BPL  J gossypiifolia is extremely diverse with many taxonomically close relatives J curcas x J scaposa  Under testing (Mozambique) J curcas x J macrocarpa  Under testing (Argentina) 9 © 2011, 2012 Bionic Fuel Knowledge Partners Inc., Oswego, NY, USA
  • Strictly confidentialSYSTEMATIC BREEDING PATH Variations for XXX Female parent Pollen donor Intraspecific: • Genetically remote JcL accessions JcL • Non-toxic JcL accessions XXX starter • Wild accessions from center of origin Interspecific: • J integerrima • Other Jatropha species F1 Hybrid • hybrids • …. BC1 F2 Inbred hybrid lines F2, F3, F4…. BC2 Fx Genetic variance has to be taken into account via permanent selection supported by genetic markers BC3 10 © 2011, 2012 Bionic Fuel Knowledge Partners Inc., Oswego, NY, USA
  • Strictly confidentialUPDATE 1: ACTUAL F1 BREEDING PATH Tested F1 are GHLT x Jint and GHOLD x Jint Female parent Pollen donor Variations for XXX Currently performed: future F1 as female parent XXX • J integerrima • GHLT • Other Jatropha • MXCLO • MXZA • …. • MXHU F2/FCF1/BCF1 • Self cross Hybrid current future F1 as pollen • MXCLO8.5/7.10 • Self cross XXX donor • MXZA • GHLT • MXHU • More NTMA • GUA1A1 • …. F2/FCF1/BCF1 • Other Jatropha Hybrid • J integerrima 11 © 2011, 2012 Bionic Fuel Knowledge Partners Inc., Oswego, NY, USA
  • POSITIVE AND PROVEN Jint JcL Joth Parental genotypes H4=JothxJcL H1=JcLxJint H4=JothxJoth 1st hybrid generations H0=JcLxJcL interspecific intraspecific H6=JothxJint intraspecificBuilding up large hybrid populations to enable full exploitation of genetic variability through selection and cross breeding H1BC1 (GAL, GHLT, CLO7.10) 2nd hybrid generations H1F2 (H1.2xH1.2) H1FC1 (H2, GUA, CLO, HU, ZA, ....) 12 © 2011, 2012 Bionic Fuel Knowledge Partners Inc., Oswego, NY, USA
  • Strictly confidentialUPDATE 2: ACTUAL JOTH RESULTSCurrent results Joth (green) x Joth (red)  intraspecific Joth x JcL (H2)  interspecific  Jgos x GHLT (H2.1)  Jgos x MXHU (H2.2) Joth x Jint  interspecificObservations Very strong maternal effect  Hybrids usually difficult to recognize  Seed texture is a reliable phenotypic indicator in most cases Intraspecific hybrids usually express some hybrid vigor (heterosis)  Leave size, plant height  Seed size, fruit size 13 © 2011, 2012 Bionic Fuel Knowledge Partners Inc., Oswego, NY, USA
  • Strictly confidentialPARENTAL MATERIAL FOR CORE APPROACH JcL Starter:  Selection from best Asian, Indian, African, South American semi commercial cultivars  Selection from non-toxic and wild Central American accessions In-crossed accessions:  Selected non-toxic  Selected wild (natural heterozygosity)  Jatropha integerima (heterozygosity)  Presumed natural hybrid from Cuba (Dehgan)  Proven good results from a chain of well documented Indian breeding experiments  Improved growth>>> We successfully produced 45 different JcLxJint hybrids during the PoC  Jatropha gossypifolia (heterozygosity)  Three distinctively different wild accessions identified in Ghana  Reported success of a fertile JcL hybrid (US patent application)  Enormous vegetative growth rate (banned as invasive in NSW, Australia)  Very strong pest resistance  Favorably shaped for mechanical harvesting 14 © 2011, 2012 Bionic Fuel Knowledge Partners Inc., Oswego, NY, USA
  • Strictly confidentialVARIATIONS OF CORE APPROACH Alternative Starters:  Jatropha other (reported success)  Jatropha integerrima  Non-toxic JcL  Wild JcL Incrossed accessions:  Selected JcL (non-toxic, wild, commercial)  Jatropha integerima (heterozygosity)  Jatropha other (heterozygosity) Induced polyploidy  First line experiments ongoing with several natural polyploid JcL plants available Targets:  New hybrids as parental material with favorable traits  Non-toxic hybrids with high heterozygosity (primarily as further breeding base)  Inter- and intraspecific  Jint/Joth hybrids as new established breeding base (patent applications pending) 15 © 2011, 2012 Bionic Fuel Knowledge Partners Inc., Oswego, NY, USA
  • TRANSGENIC APPROACH Interspecific breeding is a necessity  Limited genetic variance with globally accessible material J integerrima has ideal genetic properties  A natural hybrid from Cuba with high heterozygosity (Dehgan and others)  see variability in leaves  see variable flower colors  100% heterozygosis reported - to be verified within the program  Seeds contain high levels of linoleic acid  Fast growth and permanent flowering (3 months to first flowering reported) J other easy intraspecific crossing but difficult for interspecific hybridization  Enormously vegetative  Very high horizontal resistance  Potential for an annual crop similar to Castor development  Direct crossing with JcL (Dehgan says “impossible”)>>> we produced a limited number of healthy seeds and seedlings in 2011 forall combinations listed above, further PoC trials ongoing in 2012 establishing asound base for future mainstream breeding 16 © 2011, 2012 Bionic Fuel Knowledge Partners Inc., Oswego, NY, USA
  • NON TOXIC Why not?  Toxicity does not increase pest resistance  Some wild non-toxic accessions exhibit superior expressions of important traits A must for acceptable labor conditions  Modern crop commercialization requires strict health protection for workers at all times  Co-carcinogenic effect of PE becomes evident only after years of exposure  Any kind of detoxification process cannot eliminate that issue Fundamental turn around for weak JcL business models  Press cake forms a high quality animal feed without the need of chemical detoxification  Poultry feed  Fish feed  Potentially adds 1000 USD and more to a farms revenue stream per ha 17 © 2011, 2012 Bionic Fuel Knowledge Partners Inc., Oswego, NY, USA
  • TOOLS BEYOND HYBRIDIZATION Artificial/natural polyploidy  Checking farm for more natural occurrences  Improve experimental procedures Use of growth regulators  GA3 – coconut water  BA - Benzyladenine  Number of flowers  female/male ratio of flowers  PBZ  Induce early flowering/fruiting  Reduction of vegetative growth  Synchronous plantation cycle  Other plant enzymes  Vitamin B1- Thiamin In vitro propagation possible for important hybrids 18 © 2011, 2012 Bionic Fuel Knowledge Partners Inc., Oswego, NY, USA
  • TOOLS BEYOND HYBRIDIZATION (2) Dedicated crop cycle management  Water management  Abundant for vegetative phase (drip irrigation from reservoirs)  Mild water stress for flowering/fruiting  Synchronized pruning  PBZ as additional control instrument Soil amendments & nutrient applications  Mycorrhiza and other micro organisms show very positive results if applied correctly  Biochar additions show strong effect on vegetative growth  Enzyme treatment for faster propagation are under investigation Manipulation of day light  Research required  Extend to 16 hours for breeding nursery  Find plausible background first  Verify assumption: Best areas today are 15 - 20 degrees away from the equator  Try domestication for 0-15 degrees  Replication of Mexican daylight times 19 © 2011, 2012 Bionic Fuel Knowledge Partners Inc., Oswego, NY, USA
  • Strictly confidentialTHE BIONIC JCL BREEDING TRIANGLE oil quality > high domestication > limited seeds > medium-large genetic variability > low soil requirement > low uniformity > low drought resistance > high yield > insufficient pest resistance > moderate flowering > asynchronous non-toxic accessions > yes toxicity > medium - low market acceptance > high + _oil quality > very high Jatropha oil quality > mediumseeds > small curcas Linn seeds > very smallsoil requirement > medium soil requirement > lowdrought resistance > medium drought resistance > highpest resistance > low pest resistance > very highnon-toxic accessions > nomarket acceptance > none + + non-toxic accessions > no market acceptance > low Jatropha Otherdomestication > nonegenetic variability > high _ integerrima Jatropha _ domestication > none genetic variability > highuniformity > no uniformity > highyield > high yield > very highflowering > permanent flowering > permanenttoxicity > high toxicity > very high 20 © 2011, 2012 Bionic Fuel Knowledge Partners Inc., Oswego, NY, USA
  • Strictly confidentialTRAITS TARGETED FOR OPTIMIZATION Crop yield  Seed weight  Number of flowers & female/male ratio  Branching  Natural or pruning induced  “Willingness to flower” of new branches  Oil content & quality  Crop cycles per year Quality of crop  Toxicity  Oil composition Agronomic suitability  Uniformity  Canopy shape and size  Synchronous flowering  Drought resistance Pest resistance  Horizontal resistance 21 © 2011, 2012 Bionic Fuel Knowledge Partners Inc., Oswego, NY, USA
  • AN ELITE WILD ACCESSION FROM THE BIONIC COLLECTION A member of a family of wild germplasm collected from the center of origin  Non-toxic  Male sterile  High yielding based on several distinct traits • 12-20 fruits per cluster • 100-seed weight 85+ gram • Strong, permanent flowering • Strong branching  Cuttings develop flowers before leaves  Similarly new branch shoots from pruning often start flowers before leaves 22 © 2011, 2012 Bionic Fuel Knowledge Partners Inc., Oswego, NY, USA
  • OPEN INFORMATION EXCHANGE POLICY Bionic is promoting an open exchange policy in Jatropha plant development  Looking for collaborative partnerships  Mutually beneficial relationships  Balanced give and take  Open source approach where ever possible  Protected intellectual property only where necessary and useful We wish to talk with anyone in the industry  Open dialog without predjudice  Explore common industry  Alignment of objectives Talk to us....  exchange@bionic-palm.com 23 © 2011, 2012 Bionic Fuel Knowledge Partners Inc., Oswego, NY, USA