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PRACTICAL
MICROBIOLOGY
Gram’s stain
Gram’s stain
Gram staining (or Gram's method) is a method of
differentiating bacterial species into two large groups
Gram Positive Gram negative
Gram staining differentiates bacteria by the chemical and
physical properties of their cell walls.
Dyes and chemicals used in staining
What happens in staining process!!!
• 1. smear preparation
Procedure
Staining procedure
Results
Examples
Gram –ve bacilli Gram +ve cocci

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