Microbiology practical

1. Microbiology Practical I
The following slides were contributed
by your fellow BIO 251 students…
Use side “” key to view slides…

2. Lab 1: Eukaryotes and Microscopy
Know the parts
of the
microscope…

3. Lab 1: Eukaryotes and Microscopy
Paramecium caudatum

4. Lab 1: Eukaryotes and Microscopy
Euglena gracilis

5. Lab 1: Eukaryotes and Microscopy
Amoeba proteus

6. Lab 1: Eukaryotes and Microscopy
Fungus – Saccharomyces cerevisiae

7. Lab 1: Eukaryotes and Microscopy
Cyanobacteria ~ Nostoc

8. ~Bacteria slides~
Micrococcus luteus

9. ~Bacteria Slides~
Bacillus subtilis

10. ~Bacteria Slides~
Bacillus anthracis

11. ~Bacteria Slides~
Rhodospirillum rubrum
Sorry not in focus…

12. ~Bacteria Slides~
See other file for Gram Stain, Endospore, Acidfast,
and Capsule Staining…
[ Adobe Acrobat of Gram and Endospore ]
Some Endospore Staining ~

13. Streak Plate:
Know colony characteristics (color, elevation, margin, etc.)

14. Spread Plate vs. Streak Plate:

15. Streak Plate:

16. Spread Plate:
Mixed – Bacillus subtilis & Serratia marcescens

17. Practical I Unknown Tests:
~ Simple Stain
~ Gram Stain
~ Endospore Stain
~ Acid-Fast Stain (Demo)
~ Capsule Stain (Demo)
~ Motility (Hanging Drop, Wet Mount, Soft Agar Deep)
~ Obligate Aerobe, Facultative Anaerobe,
Aerotolerant Anaerobes, Microaerophiles
(Gas Pak, Eugon Agar, Thioglycollate Broth)

18. Gas Pak Anaerobic vs. Aerobic:

19. Dilution Problems:

20. Dilution Problems:

21. Bacterial Growth Curve:
Make sure you know
how to:
Use a
spectrophotometer
to measure
absorbance.
Produce a log graph
of the calculations.
Calculate generation
time from a growth
curve.